Breeding method for resistant super-male polyploidy asparagus based on flow cytometry

A kind of resistance and asparagus technology, applied in the biological field, can solve the problems of not having salt tolerance, drought tolerance, barrenness and disease resistance, etc., and achieve the effect of short breeding cycle, strong variety comprehensiveness and new breeding technology

Inactive Publication Date: 2012-11-21
SHAANXI TIANZHENGDA BIOLOGICAL TECH
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However, the above-mentioned varieties do not have the characteristics of

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  • Breeding method for resistant super-male polyploidy asparagus based on flow cytometry

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Embodiment Construction

[0028] The present invention is described in further detail below in conjunction with accompanying drawing:

[0029] see figure 1 , a kind of asparagus resistance super male polyploidy breeding method based on cell flow technology, according to the following steps:

[0030] (1) Select excellent male plants in the field (high yield, uniform shoot tip size, no pests and diseases), carry out indoor planting, and microscopic examination (collect unopened florets, microscopic examination, select florets with the same shape and in the uninucleate stage);

[0031] (2) Mechanical separation of microspores (pretreatment at 10°C for 7 days + hydrolyzed casein) (mechanical homogenization for 7 seconds at 9600rpm twice, filtration, 200g washing, and centrifugation to collect microspores);

[0032] (3) Perform flow sorting on the mechanically separated microspores; the step (3) is to analyze the recovered microspores under aseptic conditions by flow cytometry, and press the larger E-type ...

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Abstract

The invention discloses a breeding method for resistant super-male polyploidy asparagus based on flow cytometry. The method comprises the following steps: (1) choosing excellent male plants in a field and carrying out indoor planting and microscopic examination; (2) mechanically separating microspores; (3) carrying out flow sorting; (4) carrying out suspension culture; (5) screening resistant mutants; (6) carrying out artificial doubling; (7) carrying out flow analysis; (8) subjecting the microspores obtained in step (7) respectively to field selective matching and cross combination and to in vitro rapid propagation; and (9) carrying out variety comparative tests on plants obtained after field selective matching and cross combination and plants obtained after in vitro rapid propagation, and applying plants obtained in the tests in production in the field. The method provided by the invention has the following beneficial effects: (1) a breeding period is shorter, breeding efficiency is higher, and operation is simpler; (2) an obtained variety has comprehensiveness; and (3) a breeding technology is novel.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for breeding asparagus disease-resistant polyploidy supermale plants, in particular to a suspension induction culture method for microspore-resistant polyploids based on flow separation analysis. Background technique [0002] Free microspore culture refers to the separation of plant pollen grains in a suitable growth period by mechanical means, suspension culture on liquid medium, and then induction of pollen embryos and haploid plants, and finally artificial doubling treatment to obtain genotype high ℃ Process of homozygous double haploid offspring (DH population). Compared with the conventional method of obtaining double haploids through anther culture, free microspore culture has obvious advantages: (1) The influence of genotype is reduced, and the induction frequency is high. Microspores are unicellular and have a large population. The base number of single culture can re...

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Application Information

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IPC IPC(8): A01H4/00A01H1/02
Inventor 郭东伟
Owner SHAANXI TIANZHENGDA BIOLOGICAL TECH
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