Method for authenticating and evaluating salt tolerance of seedling-stage wheat

An evaluation method and technology for salt tolerance, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as poor accuracy, large manual errors, and high resource consumption, and achieve reliable, easy-to-master, comprehensive strong effect

Active Publication Date: 2013-12-11
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

At present, the identification of salt tolerance at the seedling stage of wheat is treated with NaCl, and the leaf growth is graded and analyzed manually. The accuracy is poor, the manual error is large, and the area is large, consuming more resources, and the leaf growth index is relatively simple. , can not comprehensively reflect the salt tolerance of wheat seedling stage, therefore, there is an urgent need for a method with high accuracy and less resource consumption to identify and evaluate the salt tolerance of wheat seedling stage

Method used

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  • Method for authenticating and evaluating salt tolerance of seedling-stage wheat
  • Method for authenticating and evaluating salt tolerance of seedling-stage wheat
  • Method for authenticating and evaluating salt tolerance of seedling-stage wheat

Examples

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Effect test

Embodiment 1

[0045]Select 16 wheat varieties with different salt tolerance and cultivate them according to the above method, and measure the four seedling salt tolerance indexes of plant height growth, root length growth, dry weight of aboveground part and dry weight of underground part, and calculate the According to the formula, the salt damage index at the seedling stage was calculated, and then according to Table 2, the 16 wheat varieties were graded and evaluated for the salt tolerance at the seedling stage. The evaluation results are shown in Table 3. From Table 3, the difference in salt tolerance index between different materials is extremely significant (P<0.01), showing that the salt tolerance of different materials is significantly different. Among them, the three new wheat lines Lian 0756, Lian 0809 and Lian 5152 all had strong salt tolerance at seedling stage, the former was grade 1 high salt tolerance, and the latter two were grade 2 salt tolerance. This may be related to the ...

Embodiment 2

[0049] Select 48 wheat varieties (lines) with good salt tolerance at the germination stage and cultivate them according to the above method, and measure the above identification indexes of salt tolerance at the seedling stage, calculate the relative value of each index, and calculate the salt damage index at the seedling stage according to the formula , and then according to Table 2, 48 wheat varieties (lines) were graded and evaluated for seedling salt tolerance, and the evaluation results are shown in Table 4. From Table 4, it can be found that the seedling salt damage index of the 48 wheat germplasms tested differs significantly (P≤0.0001), and two wheat varieties with salt tolerance at the seedling stage, Huaimai 31 and A. 18 moderately salt-tolerant wheat germplasms at the seedling stage, such as Yanfu 188 and Kefeng 1, and 28 sensitive or highly sensitive wheat germplasms at the seedling stage, which further illustrate the potential of this technology for identifying whea...

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Abstract

The invention discloses a method for authenticating and evaluating salt tolerance of seedling-stage wheat and belongs to the technical field of agricultural production. According to the method, plants are treated with manually prepared seawater, the membership value Xi of the salt damage rate of each of the four salt-tolerance indexes of the seedling-stage plant height increment, the seedling-stage root length increment, the overground part dry weight and the underground part dry weight is determined, the membership values Xi are accumulated and averaged to obtain the seedling-stage salt damage index Y, and the salt tolerance of the seedling-stage wheat is graded according to the seedling-stage salt damage index Y. The method for authenticating and evaluating the salt tolerance of the seedling-stage wheat not only is closer to actual coastal mud flat agricultural production, but also eliminates the influence of genetic backgrounds on the invested character values. The method for authenticating and evaluating the salt tolerance of the seedling-stage wheat is simple in operation, reliable in result, and capable of reflecting the salt tolerance of the seedling-stage wheat comprehensively and being effectively used for discovery of the salt-tolerance wheat variety and breeding of new varieties.

Description

1. Technical field [0001] The invention designs a method for identifying and evaluating the salt tolerance of wheat seedlings, specifically in the technical field of agricultural production. 2. Background technology [0002] According to reports, the total area of ​​salinized land in the world is about 400 million hectares, and in my country alone there are more than 70 million hectares (excluding the area of ​​secondary salinized soil), which are distributed in the main grain production areas such as Northwest China, North China and coastal areas. Agricultural production poses a serious threat. When plants suffer from salt damage, they show yellow wilt, leaves become smaller, and growth is inhibited. When the salt damage is severe, it will cause a large area of ​​crop production reduction, or even no harvest. At present, there are many studies on plant salt tolerance at home and abroad, but most of them are based on single salt (such as NaCl) or double salt (such as NaCl an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01G1/00A01G7/06
Inventor 吴纪中陈亚华刘妍妍蔡士宾张巧凤付必胜吴小有
Owner JIANGSU ACAD OF AGRI SCI
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