2results about How to "Reduced proliferative capacity" patented technology

The invention discloses application of K224 lactic acid modification of HNRNPF as a target spot in preparation of a product for diagnosis and / or treatment of pancreatic cancer, and belongs to the technical field of tumor precision medical products. The embodiment of the invention proves that the lactylation modification level of the K224th site of the HNRNPF protein in pancreatic cancer tissues is obviously higher than that of para-carcinoma tissues, the lactylation modification level of the 224th lysine of the HNRNPF protein is positively related to the progression of pancreatic cancer, and the multiplication capacity of pancreatic cancer cells can be obviously inhibited by constructing the HNRNPF K224R site mutant. On the basis, the reagent for detecting the HNRNPF K224 lactylation level can be used for preparing products for diagnosing and / or treating pancreatic cancer, such as a reagent for detecting or inhibiting the modification and the like. The application provides a novel specific marker and an intervention strategy for early diagnosis and targeted therapy of pancreatic cancer, and has important significance and application value.

This invention relates to the field of plant tissue culture technology, specifically to a culture medium and method for inducing and regenerating tiger nut callus. This invention creatively combines growth regulators (2,4-D, 6-BA, NAA, and ZT) in the culture medium for three different stages of tiger nut callus induction, regeneration, and differentiation, and optimizes the selection of the best explants. This allows each component to fully synergistically enhance its effect at each stage, resulting in high callus induction rate, good growth status, low browning degree, good proliferation effect, and high differentiation rate and differentiation coefficient. Furthermore, no growth regulators are required during rooting induction, resulting in lower costs. This invention lays the foundation for tiger nut gene function research, variety genetic improvement, and germplasm resource optimization.