82results about How to "Reduced proliferative capacity" patented technology

The invention discloses a medlar and red date enzyme and a preparation method thereof, which belong to the technical field of enzyme preparation. Multi-strain composite fungi powder is adopted as a leavening agent; the medlar and the red date are adopted as main raw materials; dietary fiber, pectin hydrolyzate, seasoner extracting liquor, Chinese herbal medicine extracting liquor, enzyme stabilizer and other multifunctional auxiliary raw materials are scientifically compounded and modified; low-temperature processing technology such as ultrasonic cleaning, cryogenic grinding, microwave-assisted high-voltage pulse electric field processing and ultrasonic ripening are mainly adopted in the whole process, so that the natural colors, the flavor, the taste and the content of the active materials of the medlar and the red date are maintained furthest, the biological stability, the non-biological stability and the biological activity of the medlar and red date enzyme are obviously enhanced, the introduction of chemical addition agent is reduced, and the juicing rate of the medlar and the red date as well as the food safety of the enzyme are improved. Finally, the medlar and red date enzyme with high biological activity, strong stability, strong taste, flavor and appetite, long expiration date and edible safety is prepared.

The invention discloses culturing, freezing and recovering methods of an activated lymphocyte with CD3+CD8+as major, which can solve problems that a patient needs carrying out blood sampling for many times caused by continuously utilizing the activated lymphocyte with CD3+CD8+as major. The method comprises the following steps of: (1) contacting the extracted lymphocyte of the peripheral blood with IL-2, IL-15, an anti-CD3 antibody and an anti-CD28 antibody, so as to amplify the activated lymphocyte with CD3+CD8+as major; (2) freezing and storing the activated lymphocyte; and (3) recovering the activated lymphocyte. The activated lymphocyte cultured via the method disclosed by the invention has clear components, and comprises few CD4+CD25+Treg cells and more CD8+T lymphocyte; feedback time and frequency of the activated lymphocyte can be adjusted according to other treatments for a patient, such as a radiotherapy or a chemotherapy, so that diseases, such as tumor, infectious diseases and immunodeficiency can be treated well.

The invention relates to polynucleotides suitable for reducing or eliminating the expression of expanded repeat RNA (CUGexp) of the dystrophy myotonic-protein kinase (DMPK) gene in a cell of a DM-1 patient. The polynucleotides are a combination of a polynucleotide for a site specific nuclease targeting the dystrophy myotonic-protein kinase (DMPK) gene locus, and a donor polynucleotide having 5′ and 3′ regions which are homologous with the sequence of DMPK gene which flank the target site of the nuclease. The invention further relate to in vivo and in vitro methods to reduce or eliminate CTG repeats in the DMPK gene. The invention further relates to the medical use of polynucleotides and cells for treating DM-1 patient.

Provided is an anti-norovirus agent that has high norovirus-inactivating activity and is safe for the human body, and an anti-norovirus composition that contains the anti-norovirus agent and is useful for disinfection and infection control against the norovirus. The anti-norovirus agent includes, as an active ingredient, an extract from a plant of the genus Diospyros containing tannin (hereinafter referred to as a “persimmon extract”), preferably a persimmon extract produced by heating squeezed juice or an extract from the fruit of a plant of the genus Diospyros or treating the squeezed juice or the extract with an alcohol. The anti-norovirus composition contains the anti-norovirus agent and at least one selected from the group consisting of alcohols, surfactants, antimicrobial agents, humectants, and cosmetic fats and oils, and preferably further containing an organic acid, such as citric acid, and / or a salt thereof or vitamin C.

The invention discloses a whitening essence. The whitening essence has no side effect, is capable of effectively inhibiting the formation of melanin and has good efficacies of preserving moisture, resisting wrinkles and removing spots. The whitening essence mainly contains the following components in by weight percent: 0.16%-1.25% of a pH adjuster, 0.01%-0.03% of a chelating agent, 3%-9% of a humectant, 0.2%-1.3% of a preservative, 0.05%-0.2% of an antioxidant, 3%-8% of a spot removal agent, 0.11%-0.6% of an emulsifying agent, 0.4%-1.5% of an emollient, 0.25%-0.8% of a thickening agent, 1%-8%of a plant extract active composition and a proper amount of water. The whitening essence belongs to the technical field of cosmetics.

The invention discloses a new application of a Chinese traditional medicine compound. The weight proportion of the raw materials of the effective ingredients during the preparation is: 308 parts of holly root, 43 parts of helicteres angustifolia, 59 parts of citrus medica, 18 parts of lophatherum gracile, 1 part of oroxylum seed, 18 parts of buzhaye, 46 parts of Chinese knotweed, 104 parts of japonicum, 27 parts of herba desmodii styracifolii and 106 parts of cherokee rose root. The invention is applicable for the preparation of the medicine, healthy food or food additive which can improve the anti-stress effect and treatment or improve the immunity weakness caused by stress and / or the sugar and fat metabolization weakness. The Chinese traditional medicine compound effectively adjusts the immunity organ atrophy resulted from the stress, the reduction of the quantity of the spleen cells, the reduction of proliferation ability of the lymph cells, the proportional disorder of the T cell sub-group in the lymph cells, and increases the activation of the NK cells in killing bacteria.

The invention discloses application of a heat shock protein gp96 in the treatment of rheumatoid arthritis. The heat shock protein gp96 is (a1) or (a2) or (a3) or (a4): (a1) a protein with an amino acid sequence represented from a site 2 to a site 783 from a terminal N in a sequence 2 in a sequence table; (a2) a protein with an amino acid sequence represented by the sequence 2 in the sequence table; (a3) a fusion protein obtained by linking a label to the terminal N or / and a terminal C of (a1) or (a2); and (a4) a protein, with the same functions, obtained through substitution and / or deletion and / or addition of one or more amino acid residue from the site 2 to the site 783 from the terminal N in the sequence 2 in the sequence table or the amino acid sequence represented by the sequence 2 in the sequence table. Experiments prove that the heat shock protein gp96 has important application values in the treatment of rheumatoid arthritis and / or the relief of symptoms of the rheumatoid arthritis.

The present invention discloses Cecropins obtained from Aedes aegypti, a coding gene and applications thereof. According to the present invention, the protein of the present invention is the following (a) or (b) or (c): (a) the protein comprises amino acid residues at sites 20-62 from the N terminal in the sequence 1 in the sequence table, (b) the protein comprises an amino acid sequence represented by the sequence 1 in the sequence table, and (c) the protein is formed by carrying out substitution, and / or deletion and / or addition of one or a plurality of amino acids on the (a) or (b), has the antibacterial peptide function, and is derived from the (a) or (b); results of the present invention further prove that the dengue virus infection capability can be effectively reduced with the AaCECN protein; and the broad application prospects are provided.

Phospholipid scramblase 3 (PLS3) is a newly recognized member of a family of proteins responsible for phospholipid translocation between two lipid compartments. A novel isoform of PLS3 is identified and characterized herein. The function of PLS3 in mitochondria was disrupted, yielding an inactive mutant PLS3(F258V). Cells transfected with PLS3(F258V) exhibited reduced proliferative capacity that was unaffected by the presence of Na3N. PLS3(F258V)-expressing cells exhibit abnormal mitochondrial metabolism and structure and were associated with decreased sensitivity to UV- and tBid-induced apoptosis, and diminished translocation of cardiolipin to the outer mitochondrial membrane. Cells transfected with wild-type PLS3 displayed increased sensitivity to apoptosis and enhanced cardiolipin translocation. These studies identify PLS3 as a regulator of mitochondrial structure and respiration, and cardiolipin transport in apoptosis.

The invention relates to a method for differentiating human umbilical cord mesenchymal stem cells into myocardial-like cells. Compared with human embryonic stem cells and bone mesenchymal stem cells, the human umbilical cord mesenchymal stem cells from Whartonps jelly have obvious advantages that cost is low, the source is abundant, and no ethical issue is involved; compared with the bone mesenchymal stem cells, the multiplication capacity and differentiation capacity of the human umbilical cord mesenchymal stem cells do not decline along with increase of the passage number and the organismic age. In this way, the myocardial-like cells differentiated from the 5-Aza induced human umbilical cord mesenchymal stem cells are in an immature stage, still have multiplication capacity and can continue multiplication to form enough myocardial cells, which provides possibility for clinical application of the myocardial-like cells in cell transplantation treatment.

The present invention relates to the treatment of autoimmune and allergic diseases by mucosal delivery by micro-organism, in particular Lactococcus lactis, of secreted immunodominant antigens.

The invention discloses a hot pot seasoning containing an antibacterial peptide; no preservatives are added, spicy fragrance is strong, spicy flavor is soft, no raw spicy flavor exists, especially with scientific compounding of a composite antibacterial peptide, conventional food preservatives are completely replaced, the composite antibacterial peptide not only contains bacteria antibacterial peptides and capsicum antimicrobial peptides, but also has a natural chili pepper fragrance and soft spicy flavor, and is quite suitable for addition of the hot pot seasoning; with scientific compoundingof a seasoning sauce, the taste and flavor of the hot pot are enhanced; the functional properties such as the antioxidant, antibacterial and pharmacological properties of the hot pot seasoning are enhanced, and comprehensive flavor substances of food materials in a roast-smoking state are collected, so the hot pot seasoning has a strong and attractive characteristic roast-smoked flavor, and a leading card of organic integration of the roast-smoked flavor and the hot pot seasoning are created; a hot pot soup prepared by the hot pot seasoning has the advantages of delicious taste, outstanding beef flavor, strong roast-smoked flavor, low cholesterol content and strong food safety, and a new shortcut is explored for preparation of the hot pot seasoning with functional characteristic flavor.

The invention relates to a natural cosmetics material capable of resisting wrinkles, and a preparation method for the natural cosmetics material, and belongs to the technical field of cosmetics materials. In the technical scheme of the invention, natural combined materials capable of resisting wrinkles can be mainly compounded, and a transdermal micro-emulsion-based gel material is assisted in fully processing skin internal cells. Meanwhile, ellagic acid substances adopted by the invention can effectively inhibit the efficacy of an elastase material, sea cucumbers are adopted to extract polypeptide materials for modification, the proliferation and the repairing of a cellular level are effectively accelerated, and skin aging is fundamentally delayed. Meanwhile, the viscosity of the micro-emulsion is improved through the gel, medicine lasting time is prolonged, medicine irritation is lowered, the natural cosmetics material can effectively act on a skin surface for a long time, and an anti-wrinkle efficacy is further improved.

The invention discloses a process for removing newborn bovine serum endotoxin. The process comprises the following steps: taking newborn bovine serum, centrifuging for 10 minutes at a speed of 4000 revolutions per minute, taking the supernatant, adding activated carbon which accounts for 0.2 percent of the mass of the supernatant for adsorbing, wherein the adsorption time is 30 minutes; centrifuging for 20 minutes at a speed of 4000 revolutions per minute after adsorption, taking the supernatant, sequentially filtering by using filter membranes with the size of 0.8mu m, 0.65mu m, 0.45mu m, 0.22mu m and 0.1mu m so as to obtain the newborn bovine serum in which the endotoxin is removed. By adoption of the activated carbon adsorption and step-by-step filtering method, the content of the endotoxin in the high-endotoxin-content bovine serum serving as wastes can be obviously reduced in the previous newborn bovine serum production process, and the production efficiency and additional value of the newborn bovine serum are improved.

The invention relates to new application of peiminine in manufacturing gastric cancer multidrug resistance reversing medicaments. The molecular formula and the molecular weight of peiminie are respectively C27H43NO3 and 429.63522. The beneficial effects provided by the invention are as follows: peiminine is used as a reserving agent; and cytotoxicity tests show that peiminine can obviously increase the sensitivity of drug resistance cells SGC7901 / VCR on chemotherapeutics when being used together with commonly applied chemotherapeutics such as VCR (vincristine), ADR (adriamycin), CDDP (cis-platinum) and 5-Fu (5-fluorouracil). The result points out that when peiminie is applied with the chemotherapeutics to inhibit proliferation of the drug resistance cells, the proliferation capacity and the drug resistance level of the drug resistance cells are reduced, IC50 (half inhibitory concentration ) of the chemotherapeutics is reduced, and the cytotoxicity is increased. Therefore, peiminine can not only inhibit the proliferation of gastric cancer cells but also has the function of reserving gastric cancer multidrug resistance.

The invention provides a preparation for inhibiting plant nematode disease. The preparation is composed of effective components, auxiliaries and fillers, wherein the effective components comprise 0.2-4wt% of trichoderma harzianum spore powder, 2%-20wt% of crowndaisy chrysanthemum root powder and 2%-10wt% of 6% oligosaccharide-chain protein. The pesticide composition is prepared from the trichoderma harzianum spore powder, the crowndaisy chrysanthemum root powder and the 6% oligosaccharide-chain protein by mixing, and the content of plant nematodes is rapidly killed from the source, so that theproduct safety quality is improved.

The invention relates to applications of tetrodotoxin in preparation of drugs used for inhibiting lung cancer cell migration and propagation, and belongs to the technical field of medicine science medicine development. The drugs capable of inhibiting lung cancer cell migration and propagation comprise 0.5 to 2<mu>g / ml of tetrodotoxin and a drug carrier, and are capable of reducing lung cancer cellmigration and propagation capacity effectively, and promoting cell apoptosis.

The invention discloses a siRNA for inhibiting SKA1 gene expression, and the length of the siRNA is 15-27 bp. The invention also discloses a recombinant vector containing siRNA, and the recombinant vector transfects the eukaryotic cell, and the cell with SKA1 gene silencing is obtained. The siRNA sequence can effectively degrade mRNA of SKA1 gene, thereby specifically inhibiting expression of SKA1 gene. The siRNA provided by the invention can be used for preparing medicaments for treating SKA1 gene related disease comprising tumour; the siRNA simultaneously can be used for researching effect of SKA1 gene in drug resistance of tumour, especially has a important meaning for clinic non-operation treatment of osteosarcoma and colorectum malignant tumor. The invention also discloses a reverse transcription PCR primer for effectively detecting SKA1 in tissue or cell, and applications of the primer in tumour diagnosis.

The present invention provides an application of metformin in preparations of drugs for treatment of Parkinson's disease (PD). Experiment results show that: with the metformin, behavioral disorders of PD model mice are significantly improved; dopaminergic neuron loss of mice midbrain substantia nigra compact part (SNc) and ventral tegmental area (VTA) due to MPTP / p is reduced; levels of dopamine (DA) and metabolites thereof in corpus striatum are improved; excessive proliferation activation of astrocyte is inhibited; and reduction of dopaminergic neuron cell line SH-SY5Y cell viability, and increases of lactate dehydrogenase (LDH) generation and reactive oxygen species (ROS) generation are inhibited, wherein the reason of the reduction of dopaminergic neuron cell line SH-SY5Y cell viability, and the increases of lactate dehydrogenase (LDH) generation and reactive oxygen species (ROS) generation is MPP<+>. In addition, the results show that the metformin provides pharmacological effects for treatment of PD. According to the application of the present invention, the metformin is adopted as an active ingredient to prepare drugs for treatment of PD.

The invention discloses a VSIG4 (V-set and Ig domain containing 4) and IGRP (glucose-6-phosphatase catalytic subunit-related protein) dual-gene coexpression recombinant adenovirus as well as a preparation method and an application thereof. The recombinant adenovirus genome contains a VSIG4 and IGRP dual-gene expression cassette which sequentially comprises a CMV (cytomegalovirus) promoter, a VSIG4 full length coding gene, a terminator, an internal ribosome entry site (IRES), an IGRP full length coding gene and a terminator from the upstream to the downstream. The preparation method of the recombinant adenovirus is simple. Dendritic cells of the recombinant adenovirus, which are transfected in vitro, are fed back in vivo, which can reduce the multiplication capacity of lymphocytes of NOD (Nonobese Diabetic) mice with diabete liability and the secretion capacity of cell factors, moreover, the onset time of the diabetes of the NOD mice is delayed, and the morbidity is reduced. The recombinant adenovirus is proved to be capable of effectively inducing the tolerance of specific T-cells and effectively inhibiting the breakage of pancreas islet beta cells and can be used for preparing dendritic cell vaccines for resisting type-1 diabetes.

The invention discloses a method for inhibiting proliferation of ovarian cancer cell. According to the method, a CRISPR / Cas9 system is utilized to edit an ATAD2 gene in an ovarian cancer cell (a cellstrain SKOV3 or a cell strain A2780) genome, so that the expression quantity and / or activity of ATAD2 protein are / is reduced; wherein the CRISPR / Cas9 system comprises sgRNA1 and sgRNA2, and a target site recognized by the sgRNA1 is a DNA molecule shown in a sequence 1 in a sequence table; wherein the target site recognized by the sgRNA2 is the DNA molecule shown as a sequence 2 in the sequence table. The experiments prove that compared with control ovarian cancer cells, the proliferation ability, the growth ability and the clone formation ability of the ovarian cancer cells with ATAD2 genes knocked out are all remarkably reduced, and the important significance is achieved for ovarian cancer treatment. The method has important application value.

The invention relates to application of a human-derived MGST1 gene in promoting proliferation of an A549 lung adenocarcinoma cell line and inhibiting apoptosis of the A549 lung adenocarcinoma cell line. According to the application, by virtue of methods such as knock-down of the A549 lung adenocarcinoma cell line by virtue of a slow virus MGST1, MTS proliferation detection, giemsa plate cloning and dyeing, flow cytometry apoptosis detection and naked mouse subcutaneous tumor formation, the influence of the MGST1 on the proliferation and apoptosis of the A549 lung adenocarcinoma cell line is judged, and results show that proliferation and cell clonality of A549 cells can be inhibited by inhibiting expression of the MGST1, and the early apoptosis of the A549 cells is promoted. A naked mouse experiment shows that the tumor volume of lung adenocarcinoma can be obviously reduced by inhibiting expression of the MGST1 and the tumor growth is inhibited.

The present invention discloses a brucellosis protecting strain. The strain is obtained by random mutation deleted gene or deleted wadC gene of S-type Brucella melitensis Rev.1. The S-type Brucella melitensis Rev.1 is used to randomly mutate the deleted gene to obtain a rough (R) Brucella RM227 with good genetic stability; and smooth (S) wadC gene Rev. 1-<delta>wadC with good genetic stability andlacking wadC gene is obtained from the S-type Brucella melitensis Rev. 1 by a homologous recombination deletion technology. The invention also provides a brucellosis RS bivalent vaccine, comprising acertain concentration ratio of rough (R) Brucella RM227 and smooth (S) Brucella Rev. 1-<delta>wadC, immunized mice experiments prove that the RS bivalent vaccine not only has better immune protectionand safety, but also can realize the differential diagnosis of natural infection antibodies and lipopolysaccharide (LPS) antibodies involved in immunity.

The invention discloses application of a carbon nanomaterial SWCNT and a derivative thereof in inhibition of cancer stem cells (CSC) and preparation of drugs for treating cancers. The application of the carbon nanomaterial SWCNT and the derivative thereof in inhibition of the cancer stem cells and preparation of the drugs for treating the cancers comprises the purposes: 1) the SWCNT and the derivative thereof can obviously inhibit the formation of the CSC; 2) the SWCNT and the derivative thereof can obviously enhance the sensitivity of cancer cells in a cancer microenvironment to chemotherapy drugs and promote the killing forces of the chemotherapy drugs; 3) the SWCNT and the derivative thereof can obviously reduce the number of the CSC in a cancer tissue and cancer blood vessel density, can obviously weaken the proliferation capacity of the cancer cell and so on and can inhibit the growth of the cancer; and 4) the SWCNT and the derivative thereof can obviously inhibit related gene expressions of a TGF(beta) signaling pathway and the downstream of the TGF(beta) signaling pathway so as to destroy the cancer microenvironment on which the CSC depends for existence. The application of the carbon nanomaterial SWCNT and the derivative thereof in inhibition of the cancer stem cells and preparation of the drugs for treating the cancers, disclosed by the invention, has the advantages that the material preparation method is simple, low in cost and easy to operate; the obtained aqueous SWCNT and the derivative thereof are specifically targeted at the cancer microenvironment, inhibit the CSC cell populations and realize wide application prospect in the treatment of the cancers.

The invention provides a serum-free culture medium for adherent cell culture and application of the serum-free culture medium, and belongs to the field of cell culture media. High-glucose DMEM (dulbecco's modified eagle media) and serum-reduced culture media are used as basic culture media, and the basic culture media contain serum substitutes, compound amino acid, penicillin and streptomycin. Theserum-free culture medium and the application have the advantages that the serum-free culture medium is free of cell culture media with animal-derived substances and accordingly is independent from animal serum; the serum-free culture medium comprises definite components, an alternative scheme for serum culture media is provided, and accordingly the serum-free culture medium is favorable for market promotion; the serum-free culture medium can be applied to the adherent cell culture; self-renewal of tumor cells and normal cell lines which are subjected to adherent culture can be maintained bythe serum-free culture medium, and long-time culture and passage can be implemented without increase of cell apoptosis proportions.

The invention provides application of an ALKBH1 gene and an expression product of the ALKBH1 gene in preparing a kit for diagnosing tumors and a drug for treating tumors. By detecting the expression situations of the ALKBH1 gene in tumor tissue of a patient suffering from stomach cancer or liver cancer, it is found that the expression level of the ALKBH1 gene is obviously improved. By building ALKBH1 gene over-expressed liver cancer and ovarian cancer cell strains to be compared with control groups respectively, the DNA6mA modification level, the multiplication capacity, the cell migration rate, the cell invasion capacity and the like of the ALKBH1 gene are obviously changed. The ALKBH1 gene and the expression product of the ALKBH1 gene are used as markers for diagnosing the tumors, so that the tumors are more accurately and quickly diagnosed; when the ALKBH1 gene and the expression product of the ALKBH1 gene are used as target genes for preparing drugs for treating the tumors, new therapeutic targets and therapy pathways are provided for treating the tumors.

The invention discloses a composition with a protection effect on blue light induced skin cell injury. The composition comprises tremella aurantialba crude polysaccharide and glucosyl hesperidin at a mass ratio of (0.01-1):(0.01-4) and more than 1: 1. The composition has a protection effect on keratinocytes injury induced by blue light, can also can promote proliferation and migration of skin keratinocytes after blue light irradiation, relieves delay of skin barrier repair caused by the blue light, and thus promotes repair of skin injury barriers. The invention further discloses use of the composition or the tremella aurantialba crude polysaccharide or the glucosyl hesperidin in preparing cosmetics. The composition or the tremella aurantialba crude polysaccharide or the glucosyl hesperidin and other active ingredients and auxiliary materials are prepared into the cosmetics, internal self-repairing of a skin barrier is facilitated, and the composition has moisturizing, relieving and repairing effects.

The invention discloses a food health-preserving tea electuary for delaying aging and belongs to the field of nutritional foods. The food health-preserving tea electuary has good effects and produces the effects fast. The food health-preserving tea electuary is prepared from mushroom, Chinese angelica, soybean, barley bran and black tea, has effects of supporting body resistance, reinforcing qi and nourishing blood, and delaying aging, and has the advantages of good effects and fast onset.

The invention discloses a method for greatly increasing the PCV2 cultivation virus titer through a streptococcus culture preparation, and belongs to the technical field of cell engineering and virus cultivation. The streptococcus culture preparation is prepared through high-temperature inactivation of streptococcus-containing supernate which is obtained after co-culture of streptococcus and anchorage-dependent cells in a maintenance culture medium. The streptococcus is streptococcus agalactiae, the cells are PK-15 cells, and the content of the streptococcus in the streptococcus-containing supernate is 100-900 million / mL. The streptococcus culture preparation is added into the maintenance culture medium according to the concentration of 0.1-1% to prepare a special culture medium used for stimulating PCV2 to generate viruses, the cultivation virus titer of the culture medium can be greatly increased by adopting the special culture medium as the maintenance culture medium for culturing PCV2, and the virus titer can reach 10<-7> or above; and a production process is greatly simplified, and the production cost is greatly lowered.

The invention discloses an aging cell model and a preparation method thereof. The preparation method of the aging cell model comprises the following steps of reducing the content or activity of multipotential stem cell ATF6, so as to obtain ATF6 deficiency multipotential stem cell; inducing the ATF6 dificiency multipotential stem cell, so as to obtain ATF6 deficiency mesenchymal stem cell, whereinthe ATF6 deficiency mesenchymal stem cell is the aging cell model; the multipotential stem cell is embryo stem cell or induced multipotential stem cell. The aging cell model has the advantages that the cell propagation ability is decreased, the recession speed is accelerated, the survival ability is decreased, the integration ability is decreased, and the protein homeostasis is unbalanced in thecell; the increasing of protein aggregate in the cell can be used for establishing an individualized medicine screening platform which is used for screening candidate (natural) compounds for regulating and maintaining adult stem cell protein homeostasis, and retarding cell ageing.