Method for predicting chemicotherapy sensitivity by using cervical carcinoma side population cell

A technology of cervical cancer cells and side group cells, which is applied in biochemical equipment and methods, microbial measurement/testing, etc., can solve the problems of insensitive radiotherapy and unsatisfactory curative effect of tumor cells

Inactive Publication Date: 2015-12-30
NANTONG TUMOR HOSPITAL
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Radiotherapy has been used in various clinical stages of cervical cancer, but its curative effect has not been improved in recent years. Some tumor cells are not very se

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for predicting chemicotherapy sensitivity by using cervical carcinoma side population cell
  • Method for predicting chemicotherapy sensitivity by using cervical carcinoma side population cell
  • Method for predicting chemicotherapy sensitivity by using cervical carcinoma side population cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] The specific embodiments of the present invention will be described below in conjunction with the accompanying drawings.

[0039] 1. Materials and equipment

[0040] 1. Sources and handling of experimental animals or materials

[0041] Human cervical cancer Hela cells were purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences (Shanghai Institute of Biological Sciences Cell Resource Center).

[0042] 2. Main instruments: CO2 constant temperature cell incubator, 96-well plate, FACS flow cytometer, inverted microscope, biological safety cabinet, ultrapure water system, centrifuge, water bath box, spectrophotometer, cell counting box-8 (CCK -8), automatic fluorescent quantitative PCR diagnostic system, linear accelerator.

[0043] 3. Main reagents: 10% fetal bovine serum (FBS), DMEM / F121:1 medium, penicillin and streptomycin mixture, trypsin, Hochest33342, PI (propidium iodide), phosphate buffer, Giemsa Dye solution, Tri...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A disclosed method for predicting chemicotherapy sensitivity by using cervical carcinoma side population cell comprises the following steps: preparing cervical carcinoma cell, and performing in-vitro adherent culture on the cervical carcinoma cell; collecting cervical carcinoma cell at logarithmic phase, and adding the cell into Hoechst33342; sorting SP cells by using a superspeed flow sorting system, and applying SP cell and NSP cell obtained through sorting to further experiment research; and then adding cis-platinum with different concentrations respectively into NSP cell and SP cell, staining by using an Annexin V FITC cell apoptosis detection kit, performing detection by using a flow cytometer, performing different doses of radiotherapy on SP cell and NSP cell, staining by using an Annexin V FITC cell apoptosis detection kit, and performing detection by using a flow cytometer. The cell apoptosis rate is equal to UR% plus LR%. Cervical cancer stem cell screening is performed by taking SP cell as an entry point, chemoradiotherapy sensibility difference and possible mechanism are analyzed, and theoretical basis is provided for researching and developing tumor stem cell resisting medicines, some cytokines interdicting signal path and chemical substances.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for predicting sensitivity to radiotherapy and chemotherapy by using cervical cancer side population cells. Background technique [0002] Cervical cancer is the second most common malignant tumor in women worldwide, second only to breast cancer. There are about 100,000 new cases of cervical cancer in my country every year, accounting for about 1 / 5 of the new cases of cervical cancer in the world. In developing countries, the death rate of women's cervical cancer exceeds that of breast cancer, ranking first among the causes of death from malignant tumors in women. In recent years, the morbidity and mortality of cervical cancer in some areas of our country have been increasing, and some areas have also shown a trend of younger patients. The treatment of cervical cancer adopts a comprehensive treatment plan based on surgery and radiotherapy, supplemented by chemotherapy....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/02
Inventor 何爱琴章伟玲刘蓉陈曾燕何陈云吴霞贾美群季瑞徐海波
Owner NANTONG TUMOR HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap