The invention discloses a method for observing the transferring of a fusion protein to a rice leafbud at long distance. The method comprises the following steps of: treating a root tip of rice by using a GFP (Green Fluorescent Protein) fusion protein; then, freezing a leafbud of a rice plant with a treated root tip, and slicing; next, dyeing by using a PI (Propidium Iodide) fluorescent dye; and directly observing the transferring condition of the GFP fusion protein from the root tip to the rice leafbud by using a confocal microscopy. By using the method, the position, 0.5cm away from the root tip, of the rice is treated by using the GFP fusion protein, the leafbud is frozen and sliced and then dyed, and the transferring condition of the GFP fusion protein from the root tip to the rice leafbud is observed by using the confocal microscopy, so that the long-distance transferring condition of the GFP fusion protein in the rice plant can be directly and qualitatively known about. The position, 0.5cm away from the root tip of the rice, is directly treated by using the GFP fusion protein, the transferring of the GFP fusion protein to the leafbud is observed by using the confocal microscopy, and thus, a very powerful evidence can be further provided for the fact that an effect protein can induce the defensive reaction of a rice system. The method is simple, convenient, accurate and rapid.