5 results about "Propidium iodide" patented technology

Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells. PI binds to DNA by intercalating between the bases with little or no sequence preference. Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. Propidium Iodide is not membrane-permeable, making it useful to differentiate necrotic, apoptotic and healthy cells based on membrane integrity. PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining.