Semen energy quick detection reagent kit and its preparation and usage method

A detection kit and technology for sperm motility, applied in biochemical equipment and methods, measuring devices, microbe determination/inspection, etc., can solve problems such as delayed treatment, limited and cumbersome conditions for sperm retrieval, and save time and use simple effect

Active Publication Date: 2011-01-26
北京望升伟业科技发展有限公司 +1
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Problems solved by technology

But this method has many shortcomings, for example: due to the traditional Chinese concept, it is embarrassing to go to the hospital for diagnosis and treatment of male infertility diseases, thus delaying the treatment
Even if you go to the hospital, it is not easy to obtain samples due to the limited conditions for sperm collection, limited by the environment, methods, and time
It is cumbersome to go to the hospital to see a doctor. You must go through a series of procedures such as registration and prescription. There is also a test kit for detecting sperm motility, which can only detect the number of all sperm, that is, the total number of viable and non-viable sperm. The number of viable spermatozoa has limited reference significance for diagnostic results

Method used

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  • Semen energy quick detection reagent kit and its preparation and usage method
  • Semen energy quick detection reagent kit and its preparation and usage method

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Embodiment Construction

[0017] Test plate of the present invention comprises semen collection box, triton X-100 solution, propidium iodide solution, standard color card and test plate, and described semen collection box is made up of plastic collection box and semen liquefaction agent, and described The test board includes a shell 2 and a base plate 1. The shell 2 and the base plate 1 are made of rectangular PVC plastic. There are two test holes in the center of the shell 2. There are absorbent paper 6, filter membrane 5, absorbent paper 6, and filter membrane on the base plate. 5 is located above the base plate 1 and below the two test holes of the shell 2; the absorbent paper 3 is located below the filter membrane 5; the colors of the standard color card are the sperm samples with different concentrations confirmed by microscope counting, respectively adding 0.05-0.2% Qula The color of the test results of the solution and the concentration of 0.01-0.5% propidium iodide solution. The filter membrane...

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Abstract

The invention discloses a spermatozoon vigor fast detection kit and preparing and using method thereof. The kit includes a spermatozoon collecting box containing a plastic collecting box and spermatozoon liquefacient; triton X100 solution; propidium iodide solution; a standard color card, colors of which are color of adding triton solution with concentration of 0.05-0.2% and propidium iodide solution with concentration of 0.01-0.5% into spermatozoon sample with different concentration notarized by microscope; and a test board containing a shell and a basal plate, wherein two test pores are arranged on center of the shell, absorbent paper and filtering film are equipped on the basal plate. Advantages of the kit are: quantity of activated spermatozoon can be fast and accurately detected, more time can be saved than traditional diagnostic method, and usage is simple.

Description

technical field [0001] The invention relates to an in vitro diagnostic medical device, in particular to a rapid detection kit suitable for male sperm motility. Background technique [0002] Fertility experts report that about 15% of couples in the world cannot conceive within a year (without contraceptive measures), and 40% of these cases are caused by male factors. Andrology experts report that the incidence of male infertility is about 6-12%. The above-mentioned reports indicate that the population of male infertility is quite large. [0003] At present, the main method for diagnosing male infertility is the diagnosis of semen under a microscope, that is, sperm morphology detection, which is also the gold standard for the diagnosis of male infertility. But this method has many shortcomings, for example: due to the traditional Chinese concept, it is embarrassing to go to the hospital for diagnosis and treatment of male infertility diseases, thus delaying the treatment. E...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/483C12Q1/04
Inventor 周明非赵永岐刘淑红范明魏华周明东李娅
Owner 北京望升伟业科技发展有限公司
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