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Semen energy quick detection reagent kit and its preparation and usage method

A technology for detecting kits and sperm motility, applied in biochemical equipment and methods, measuring devices, and microbial determination/inspection, etc., can solve problems such as delayed treatment, limited conditions for sperm collection, and limited reference significance, and save time. , using simple effects

Active Publication Date: 2007-10-17
北京望升伟业科技发展有限公司 +1
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Problems solved by technology

But this method has many shortcomings, for example: due to the traditional Chinese concept, it is embarrassing to go to the hospital for diagnosis and treatment of male infertility diseases, thus delaying the treatment
Even if you go to the hospital, it is not easy to obtain samples due to the limited conditions for sperm collection, limited by the environment, methods, and time
It is cumbersome to go to the hospital to see a doctor. You must go through a series of procedures such as registration and prescription. There is also a test kit for detecting sperm motility, which can only detect the number of all sperm, that is, the total number of viable and non-viable sperm. The number of viable spermatozoa has limited reference significance for diagnostic results

Method used

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  • Semen energy quick detection reagent kit and its preparation and usage method

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Embodiment Construction

[0017] The test board of the present invention includes a semen collection box, Triton X-100 solution, propidium iodide solution, standard color card and a test board. The semen collection box is composed of a plastic collection box and a semen liquefaction agent. The test board includes a shell 2, a substrate 1. The shell 2 and substrate 1 are made of rectangular PVC plastic. There are two test holes in the center of the shell 2. There are absorbent paper 6, filter membrane 5, absorbent paper 6 and filter membrane on the substrate. 5 is located above the substrate 1 and below the two test holes of the housing 2; the absorbent paper 3 is located below the filter membrane 5; the color of the standard color card is the sperm samples of different concentrations confirmed by the microscope counting and adding the concentration of 0.05-0.2% trituration Pass the color of the test result of the solution and the concentration of 0.01-0.5% propidium iodide solution. The filter membrane is ...

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Abstract

The invention discloses a spermatozoon vigor fast detection kit and preparing and using method thereof. The kit includes a spermatozoon collecting box containing a plastic collecting box and spermatozoon liquefacient; triton X100 solution; propidium iodide solution; a standard color card, colors of which are color of adding triton solution with concentration of 0.05-0.2% and propidium iodide solution with concentration of 0.01-0.5% into spermatozoon sample with different concentration notarized by microscope; and a test board containing a shell and a basal plate, wherein two test pores are arranged on center of the shell, absorbent paper and filtering film are equipped on the basal plate. Advantages of the kit are: quantity of activated spermatozoon can be fast and accurately detected, more time can be saved than traditional diagnostic method, and usage is simple.

Description

Technical field [0001] The invention relates to an in vitro diagnostic medical device, in particular to a rapid detection kit suitable for male sperm motility. Background technique [0002] Fertility experts report that about 15% of couples in the world cannot get pregnant within a year (without contraceptive measures), and 40% of these cases are caused by male factors. Andrology experts reported that the incidence of male infertility is about 6-12%. The above reports indicate that the male infertile population is quite a large population. [0003] At present, the main method for diagnosing male infertility is the microscopic diagnosis of semen, that is, sperm morphology testing, which is also the gold standard for male infertility diagnosis. However, this method has many shortcomings. For example, due to the traditional Chinese concept, I am embarrassed to go to the hospital for treatment of male infertility diseases, which delays the treatment. Even if I go to the hospital, it ...

Claims

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Application Information

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IPC IPC(8): G01N33/483C12Q1/04
Inventor 周明非赵永岐刘淑红范明魏华周明东李娅
Owner 北京望升伟业科技发展有限公司
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