179 results about "Spermatozoon" patented technology

The invention discloses a bull sperm motility related SNP molecular marker based on an HSPB9 gene, a detection product and application, and belongs to the technical field of molecular biology, the SNP molecular marker is located at the position of a bovine genome Chr19: 42255643, and the polymorphism is C or T. The research finds that the SNP genotype combination with high sperm motility of the bull is TT. By detecting the SNP of the SNP site, the sperm motility evaluation of the bull can be realized, the breeding is assisted, a reliable support is provided for breeding a high-quality next generation, and the method has the advantages of high reliability, high efficiency, low cost and the like.

The present invention provides a method for accurately measuring live sperm morphology, including the following steps: S11. acquiring an image including a plurality of sperm cells; S12. detecting the sperm cells in the image, and segmenting a head, a midpiece, and a tail of each detected sperm cell; and S13. calculating morphological parameters of each sperm cell based on a segmentation result. For the method for accurately measuring live sperm morphology of the present invention, an image of sperm cells is acquired, a head, a midpiece, and a tail of each sperm cell are segmented through image processing, and morphological parameters of each sperm cell are calculated based on a segmentation result. Morphological parameters of live sperm cells can be comprehensively and accurately obtained for analysis or selection.

The invention provides a method for cultivating hybrid fries of Jinhu and tiger. The method comprises the following steps: selecting a breeding environment; breeding and ripening female parents; management and control before oviposition; insemination; hatching fertilized eggs; fry rearing. According to the method, in the female parent breeding and ripening stage, specific biological bait is controlled to be fed in stages, feeding of the parent fish can be promoted, growth can be accelerated, in cooperation with specific compound feed in the management and control stage before oviposition, favorable conditions are created for subsequent oviposition, and the quality and number of ova can be improved. Low-frequency ultrasonic assisted fertilization is introduced in the artificial insemination stage, so that the activity of ovum sperms can be obviously improved, and the fertilization rate is increased; in the fertilized egg hatching stage, intermittent ultrasound is assisted, the hatching rate can be increased, and the seedling yield of the golden-tiger hybrid spots can be increased; after hatching, transferring into a specific culture pond, slowly releasing beneficial bacteria, improving the adaptability of the fry, adsorbing harmful substances, reducing pathogenic bacteria, stabilizing water quality, promoting the growth of the fry, improving the survival rate and reducing the malformation rate.

The present invention provides an automatic semen analysis method based on multi-frame information fusion with occlusion awareness. The present invention first proposes the combination of an edge-sensitive U-Net model and an occlusion-aware tracker based on joint probabilistic data association to achieve accurate and stable sperm detection and tracking. Thanks to the pixel-level contour segmentation and the overlap inference based on multi-frame target contours of the present invention, the present invention can accurately distinguish two or more spermatozoa that are close but not overlapping, and provide accurate head positioning; can accurately predict and judge the occurrence, progress, and end of target overlap; and can effectively match spermatozoa before and after overlap to ensure the consistency of tracking. The present invention can be conveniently integrated into existing computer-aided sperm analysis systems to provide semen analysis data with better accuracy and robustness.

The invention provides a sperm automatic detection and tracking method, sperm automatic detection and tracking equipment and a storage medium. The method comprises the following steps: firstly, preprocessing a sperm image, performing rotation bounding box labeling on a head and a tail, and generating a training set containing a center point coordinate, a width, a height and an angle; training a target detection model by using the training set to obtain a model capable of automatically identifying sperms and outputting a rotating bounding box; detecting video frames by using the model, and extracting the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and track continuity, adjacent frame targets are matched, a comprehensive score is generated, the targets are associated, and a continuous track and a unique identifier are formed and used for calculating motion parameters; and judging whether the track is static or low active according to the speed and displacement of the latest N frames, sealing the track to eliminate subsequent matching, and only reserving a non-sealed track to calculate motion parameters. According to the method, interference is effectively avoided, and the tracking accuracy and the reliability of sperm motility parameter calculation are remarkably improved.

The invention discloses an application of luteolin in improving the reproductive performance of cocks. Researches show that in the breeding process of the cocks, the breeding performance of the cocks can be effectively improved by feeding the luteolin. Test data show that the luteolin can significantly improve the cock semen collection amount, improve the sperm density and sperm motility, significantly improve the diameter of a fine sperm tube and a spermatogenic cell matrix layer, and artificial insemination of the test cock semen improves the hatching egg fertilization rate, the hatching rate and the healthy chick rate. Therefore, the luteolin has important significance in research and development of related products for improving the reproductive performance of the cocks, and the luteolin has great potential in preparation of medicines, feeds or feed additives for improving the reproductive performance of the cocks.

According to the Hainan pig semen preservation diluent as well as the preparation method, the application and the detection method thereof disclosed by the invention, galangin and kaempferide are added into a basic diluent to develop a diluent formula capable of efficiently preserving semen at a low temperature (4 DEG C); the galangin and the kaempferol added into the diluent have a good sperm protection effect and a certain antibacterial ability and can replace antibiotics, and bovine serum albumin (BSA) is further combined as a low-temperature protective agent, so that sperm movement parameters are improved, and the low-temperature preservation time of the boar semen is effectively prolonged; the use amount of antibiotics in pig raising production is reduced, the problem of drug resistance of bacteria can be effectively relieved, and the use of the antibiotics and the generation of drug-resistant bacteria are greatly reduced. According to the method, the semen utilization rate of excellent breeding boars is increased, sow health is guaranteed, the conception rate of deep insemination is increased, and efficient and low-cost artificial insemination is achieved.

Systems for sorting sperm include a housing and a microfluidic system supported by the housing. The systems also include two or more inlets providing access to the microfluidic system to deliver sperm or semen and fluid to the microfluidic system, as well as an outlet for harvesting sorted sperm. The systems include a flow channel that provides a flow path for sperm from an inlet to an outlet while sperm travels against a fluid flow towards an outlet for harvesting. In the systems, fluid delivered to the microfluidic system via an inlet flows from the inlet towards one or more outlets, and the flow channel extending along the length of the microfluidic system provides a flow path for motile sperm to travel against the fluid flow towards a collection outlet for harvesting. In some embodiments of a system, the microfluidic system also includes a microporous filter arranged in the flow path between the inlet and the collection outlet to cause motile sperm traveling against the fluid flow to pass through the filter to reach the collection outlet for harvesting.

The invention provides a recombinant lentivirus capable of indicating bovine spermatogonial stem cell differentiation, a spermatogonial stem cell line and application, and belongs to the technical field of cell biology. The invention provides a method for constructing a spermatogonial stem cell line for indicating bovine sperm differentiation on the basis of a lentivirus transduction technology, and a visual detection system for differentiating spermatogonial stem cells into spermatoblasts is constructed by regulating expression of mCherry protein by utilizing a PRM1 promoter. According to the method, the expression of PRM1 is indicated by fluorescent protein mCherry, so that the differentiation process of the bovine spermatogonial stem cells is indicated, and a good tool is provided for the induction of differentiation of the bovine spermatogonial stem cells to sperms, the research of a development mechanism and the optimization of a differentiation system of the bovine spermatogonial stem cells. The method is a new way for solving the neck clamping problem that good-variety bovine sperms are difficult to obtain in scientific research and production, can also be used for optimization of a spermatogonial stem cell differentiation system, sorting and purification of sperms, even downstream research of drug screening and the like, and has a good application prospect.

A discrimination system with optics that collect output electromagnetic radiation from sperm cells within a measurement region, a split detector that generates an electrical signal responsive to the intensity of the collected electromagnetic radiation and which detects the position of sperm cells in the measurement region, and an analyzer for discriminating between X-chromosome bearing sperm and Y-chromosome bearing sperm in the measurement region.

The application provides a sperm automatic detection and tracking method, device and storage medium. The method first pre-processes a sperm image, performs head-tail rotation boundary box labeling, and generates a training set containing center point coordinates, width, height and angle; a target detection model is trained using the training set to obtain a model capable of automatically identifying sperm and outputting a rotation boundary box; the model is applied to detect video frames to extract the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and trajectory continuity to match adjacent frames of targets, generate a comprehensive score and associate the targets to form a continuous trajectory and a unique identifier for calculating motion parameters; and then whether the trajectory is stationary or low active is judged according to the speed and displacement of the last N frames, the trajectory is sealed to exclude subsequent matching, and only the non-sealed trajectory is retained to calculate the motion parameters. The method effectively avoids interference, significantly improves the tracking accuracy and the reliability of sperm motility parameter calculation.

The invention discloses a micro-fluidic chip for exploring in-vitro XY type sperm fertilization preference, and relates to the technical field of biomedical engineering.According to the technical scheme, the micro-fluidic chip is characterized by comprising a chip body, the chip body is provided with a central sample carrying hole, the central sample carrying hole is used for containing an oocyte, and the central sample carrying hole is communicated with the central sample carrying hole; the chip body is provided with four peripheral sample carrying holes, the four peripheral sample carrying holes are used for placing fluorescently-labeled sperm cells subjected to sexual control sorting, and communicated micro-channels are arranged between the central sample carrying hole and the four peripheral sample carrying holes. Observation is facilitated by improving the volume of a liquid carrying hole and optimizing the transparency of a material, meanwhile, by adjusting the diameter of a channel and the number of repeated units, the thickness of the micro-fluidic chip is reduced, micro-channel blockage is avoided, the number of cells in loaded liquid is guaranteed, and the detection accuracy of the micro-fluidic chip is improved. The preference of successful fertilization of X and Y type sperms under different conditions is observed in vitro through micro-fluidic, and a new thought is provided for a method for controlling the gender of a fetus.

An automated method for assessing the ZP binding capacity of sperm from morphological characteristics of sperm in assisted reproduction using deep learning is disclosed. The invention also provides a method for predicting fertilization success based on ZP binding capacity of sperm in clinical assisted reproduction.

This invention belongs to the field of membrane separation and functional membrane materials technology, and discloses a chiral graphene oxide-based separation membrane, its preparation method, and its application. The membrane is composed of graphene oxide and / or reduced graphene oxide sheets and a single-stranded DNA-single-walled carbon nanotube complex. The single-stranded DNA helically winds around the outer surface of the single-walled carbon nanotubes to form a one-dimensional chiral nanochannel and intercalates between the sheets, forming a three-dimensional chiral channel network connected in the membrane thickness direction. The preparation method includes: heating and decohexing salmon sperm double-stranded DNA in a urea-containing buffer to obtain single-stranded DNA; dispersing single-walled carbon nanotubes and then complexing them with the single-stranded DNA; further mixing with a graphene oxide or reduced graphene oxide dispersion; and vacuum filtration to form the membrane. This membrane exhibits a high separation factor for chiral enantiomers such as L / D-phenylalanine, with an excess value of up to 99.95% for L-phenylalanine enantiomers after multi-stage separation, making it suitable for the efficient separation of chiral drugs and amino acids.

The present invention relates to the field of genetic breeding technology, and specifically discloses a SNP molecular marker, primer, and application related to linear motility of sperm after cryopreservation and thawing of chicken semen. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO.1, and the 53rd position in the SNP molecular marker is a SNP site, which has a G / A polymorphism. The SNP molecular marker provided by the present invention has a SNP site at position 53, which has a G / A polymorphism. The proportion of linearly motile sperm in semen of individuals with a GA genotype after cryopreservation and thawing of semen is higher than the proportion of linearly motile sperm in semen of individuals with an AA genotype after cryopreservation and thawing of semen.

A method for characterizing a mobile particle in a sample (10), the method comprising: acquiring at least one image () of the sample during an acquisition period, using an image sensor (20) and forming a series of images, the series of images comprising at least one image; using each image of the series of images resulting from a) as an input image () of a convolutional neural network for detection (CNNd), the convolutional neural network for detection being configured to detect the particles and to produce, from each image, an output image () on which each detected particle is assigned an intensity distribution, centered on the particle and extending around the particle; for each detected particle, from each output image () resulting from b), estimating a position of each detected particle in each image of the series of images;characterization of each detected particle from the estimate of the position resulting from c), established from each image of the series of images.;

1. Name of the product in this design: Sperm Quality Analyzer. 2. Purpose of this design: The product is intended for use by research institutions and medical institutions for sperm concentration detection, motility analysis, sperm morphology identification, and determination of semen pH and DNA fragmentation rate in semen samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D rendering 2.

A method of artificial-intelligence-based robotic pipetting for spermatozoa preparation includes positioning a vessel containing a semen sample within a staging mechanism. The method includes using a robotic pipettor to make at least two droplets within a dish on the staging mechanism. The method includes using the robotic pipettor to connect the at least two droplets with a medium channel according to a programmable design commanded by an artificial intelligence / machine learning system (AI / ML system). The method includes using the robotic pipettor to deposit a quantity of sperm from the semen sample into one of the droplets. The method includes using the AI / ML system to optically scan the quantity of sperm to produce first and second image objects using an imaging system that includes a microscopy system, a camera system, and a lighting system. Creation of the image objects is separated by a specified time duration.

The invention discloses a yak frozen semen freezing process intelligent control method based on dynamic cooling curve optimization, and relates to the field of animal frozen semen preservation, and the yak frozen semen freezing process intelligent control method comprises the following steps: arranging a plurality of frozen semen tubes filled with consumed cattle semen on an annular tray with a cold source in the center in a radioactive manner, and uniformly arranging the plurality of frozen semen tubes in a surrounding manner in each radial distance interval; in the freezing process, cold energy is output according to a preset cooling program, and the annular tray is driven to rotate; unfreezing operation is executed, sperm motility values are measured, interval motility values of the sperm motility values of the frozen sperm tubules in all the radial distance intervals are counted, a temperature curve at the radial distance interval with the highest interval motility value is determined, a cooling program is modified in multiple rounds of experiments, and the process is repeated, so that the temperature curve is determined. And clustering the obtained temperature curves and selecting an optimal target temperature curve to perform freezing control. According to the method, the problem that the optimal dynamic cooling curve is difficult to efficiently obtain in the freezing process of the frozen yak sperms is solved.

The invention belongs to the technical field of medicines, and discloses a gastrodia elata microbial fermentation product as well as a preparation method and application thereof. According to the invention, three aspergillus oryzae are utilized to successfully carry out microbial fermentation on gastrodia elata, the gastrodia elata microbial fermentation product of which the content of active ingredients gastrodin and p-hydroxybenzyl alcohol is obviously increased is obtained, and the pharmacological action of the gastrodia elata microbial fermentation product on treating or improving oligoasthenospermia is verified through an oligoasthenospermia mouse model; results show that the gastrodia elata microbial fermentation product has better effects of improving sperm concentration, enhancing sperm motility and rapidly moving forwards, can be used as an active ingredient of a medicine for treating or improving oligospermia and asthenospermia, and has wide application.

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to the sperm acrosome intact rate of fresh sperm of a local breeding rabbit in Fujian and application of the SNP molecular marker. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID No: 1; the 94th basic group from the 5'end in the nucleotide sequence of the SNP molecular marker is represented by M, M represents A or G. The locus is related to the sperm acrosome integrity rate of the fresh sperm of the local breeding rabbit in Fujian. The fresh semen sperm acrosome intact rate of the local breeding Fujian rabbits with the genotype of homozygous GG at the site is obviously lower than that of the local breeding Fujian rabbits with the genotype of homozygous AA and heterozygous AG at the site. The SNP molecular marker disclosed by the invention is closely related to the sperm acrosome intact rate of the fresh semen of the local breeding rabbits of Fujian, and can be effectively used for molecular marker-assisted breeding of the local breeding rabbits of Fujian. The established detection method can be used for rapidly, efficiently and accurately detecting the sperm acrosome integrity rate of the fresh semen of the Fujian local breeding rabbits.

The invention discloses an application of gallnut tannic acid as an additive in boar production. The application of the gallnut tannic acid as the additive comprises the application of the gallnut tannic acid as a feed additive in improving the reproductive performance of adult breeding boars and the application of the gallnut tannic acid as a semen diluent additive in improving the normal-temperature preservation quality of boar semen. The feed additive can improve the reproductive performance of adult breeding boars, can significantly improve the sperm density, the total sperm number and the effective sperm number of the boars after being fed for 12 weeks, and improves the oxidation resistance of sperms, sperms and serum; the semen diluent additive can improve the normal-temperature preservation quality of the boar semen, and the sperm motility can still reach 61.20% after the boar semen is preserved for 7 days.

The invention belongs to the technical field of biology, and particularly relates to an acrossocheilus fasciatus sperm cryopreservation method which comprises the following steps: constructing a simulated water solution for acrossocheilus fasciatus in-vitro fertilization, collecting fresh sperms, detecting the survival rate, preparing a mixed solution of the sperms and anti-freezing components, sub-packaging, cooling and freezing and the like. According to the method, simulated water quality for in-vitro fertilization of acrossocheilus longifin is constructed, the survival rate of sperms observed and detected in the environment is closer to the fertilization condition during thawing activation in the future, and then the sperms with the higher fertilization success rate after thawing are screened out; meanwhile, the constructed simulated water solution can be used as a part of a cryopreservation solution, so that the remaining anti-freezing components can be directly added into the screened sperms, a mixed solution of the sperms and the anti-freezing components can be rapidly prepared under the condition that the service life of the sperms is not remarkably shortened, and the mixed solution is subpackaged, cooled and frozen; the improved anti-freezing component contains trehalose, soybean phospholipid and tea polyphenol, so that the survival rate and activity of the acrossocheilus fasciatus sperms after cryopreservation can be effectively improved.

The present invention relates to the identification and / or selection of sperm, and in particular a means for identifying and / or selecting the fittest sperm for use in assisted reproductive technologies.

The invention discloses a preparation for improving male erectile function and sperm quality. The health-care food is characterized by being prepared from the following raw materials in parts by weight: 30-50 parts of herba epimedii extract, 20-40 parts of herba cistanche extract, 15-30 parts of fructus lycii extract, 18-35 parts of semen cuscutae extract, 10-25 parts of herba cynomorii extract, 8-20 parts of maca powder, 6-18 parts of raspberry extract, 0.5-2 parts of lycopene, 1-3 parts of L-carnitine and 0.3-1 part of vitamin B family (vitamin B1, vitamin B2, vitamin B6 and vitamin B12 are mixed according to the mass ratio of 1: 1: 2: 0.5). The herba epimedii extract is selected as a main component, the content of icariin is regulated to be not lower than 60%, the high-content icariin can more effectively promote sex hormone secretion, enhance sexual desire and improve erectile function, the total content of echinacoside and verbascoside in the herba cistanche extract is not lower than 45%, and the herba epimedii extract and the herba cistanche extract have the effect of promoting sex hormone secretion, enhancing sexual desire and improving erectile function. The two components have the effects of tonifying kidney yang and benefiting essence and blood, and can enhance kidney functions.

The application relates to a sperm posture and motion state control method. The control method comprises the following steps: S1: using two laser beams to act on the head of a sperm; S2: adjusting the positions and powers of the two laser beams acting on the head of the sperm; the sperm can be controlled at a selected position, and the posture (referring to the relative position and angle between the head of the sperm and an imaging focal plane) and motion state of the sperm are controlled to obtain images of sperms in different directions and different motion states, wherein the posture and motion state of the head of the sperm and the relative focal plane of the sperm tail are included. The sperm relative to the imaging plane is controlled in the posture and motion state by adjusting the positions and powers of the two laser beams acting on the head of the sperm; for example, the motion of the sperm tail in a plane parallel to the imaging focal plane is controlled by controlling the posture of the sperm, and the motion images of the head of the sperm and the tail in a three-dimensional space are observed and obtained for a long time.

The invention provides an SNP (Single Nucleotide Polymorphism) marker for influencing the rapid forward movement sperm proportion of a pig. The sequence of nucleic acid of the SNP marker is shown as SEQ ID No.1, and the site of the SNP marker is located at the 301st site from the 5'end on the nucleic acid shown as SEQ ID No.1, corresponds to the 3803902 site from the 5 'end on the NW018084979.1 chromosome of the international pig genome of the version 11.1, and is C or T.

The invention relates to a chilo suppressalis seedless sperm formation factor TNNI3 and application thereof in male sterility. The invention finds that the TNNI3 gene specifically regulates and controls the chilo suppressalis seedless spermatogenesis process for the first time, and an RNA interference technology proves that TNNI3 can inhibit the number of chilo suppressalis seedless sperm bundles and has no obvious influence on the number of nucleated sperm bundles and sperm motility, so that the egg laying amount after mating of male insects and wild female insects can be obviously reduced, and the survival rate of the male insects and the wild female insects is improved. And a theoretical basis is provided for developing a novel prevention and treatment strategy for male reproduction.

The invention relates to a method for in vitro or ex vivo diagnosis of male infertility, resulting from a dysfunction of the lipid metabolism of spermatozoa, in an individual, as well as a kit and the use of one or more specific marker(s) for this diagnosis.