124 results about "Spermatozoon" patented technology

The invention provides a sperm automatic detection and tracking method, sperm automatic detection and tracking equipment and a storage medium. The method comprises the following steps: firstly, preprocessing a sperm image, performing rotation bounding box labeling on a head and a tail, and generating a training set containing a center point coordinate, a width, a height and an angle; training a target detection model by using the training set to obtain a model capable of automatically identifying sperms and outputting a rotating bounding box; detecting video frames by using the model, and extracting the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and track continuity, adjacent frame targets are matched, a comprehensive score is generated, the targets are associated, and a continuous track and a unique identifier are formed and used for calculating motion parameters; and judging whether the track is static or low active according to the speed and displacement of the latest N frames, sealing the track to eliminate subsequent matching, and only reserving a non-sealed track to calculate motion parameters. According to the method, interference is effectively avoided, and the tracking accuracy and the reliability of sperm motility parameter calculation are remarkably improved.

Systems for sorting sperm include a housing and a microfluidic system supported by the housing. The systems also include two or more inlets providing access to the microfluidic system to deliver sperm or semen and fluid to the microfluidic system, as well as an outlet for harvesting sorted sperm. The systems include a flow channel that provides a flow path for sperm from an inlet to an outlet while sperm travels against a fluid flow towards an outlet for harvesting. In the systems, fluid delivered to the microfluidic system via an inlet flows from the inlet towards one or more outlets, and the flow channel extending along the length of the microfluidic system provides a flow path for motile sperm to travel against the fluid flow towards a collection outlet for harvesting. In some embodiments of a system, the microfluidic system also includes a microporous filter arranged in the flow path between the inlet and the collection outlet to cause motile sperm traveling against the fluid flow to pass through the filter to reach the collection outlet for harvesting.

The application provides a sperm automatic detection and tracking method, device and storage medium. The method first pre-processes a sperm image, performs head-tail rotation boundary box labeling, and generates a training set containing center point coordinates, width, height and angle; a target detection model is trained using the training set to obtain a model capable of automatically identifying sperm and outputting a rotation boundary box; the model is applied to detect video frames to extract the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and trajectory continuity to match adjacent frames of targets, generate a comprehensive score and associate the targets to form a continuous trajectory and a unique identifier for calculating motion parameters; and then whether the trajectory is stationary or low active is judged according to the speed and displacement of the last N frames, the trajectory is sealed to exclude subsequent matching, and only the non-sealed trajectory is retained to calculate the motion parameters. The method effectively avoids interference, significantly improves the tracking accuracy and the reliability of sperm motility parameter calculation.

An automated method for assessing the ZP binding capacity of sperm from morphological characteristics of sperm in assisted reproduction using deep learning is disclosed. The invention also provides a method for predicting fertilization success based on ZP binding capacity of sperm in clinical assisted reproduction.

This invention belongs to the field of membrane separation and functional membrane materials technology, and discloses a chiral graphene oxide-based separation membrane, its preparation method, and its application. The membrane is composed of graphene oxide and / or reduced graphene oxide sheets and a single-stranded DNA-single-walled carbon nanotube complex. The single-stranded DNA helically winds around the outer surface of the single-walled carbon nanotubes to form a one-dimensional chiral nanochannel and intercalates between the sheets, forming a three-dimensional chiral channel network connected in the membrane thickness direction. The preparation method includes: heating and decohexing salmon sperm double-stranded DNA in a urea-containing buffer to obtain single-stranded DNA; dispersing single-walled carbon nanotubes and then complexing them with the single-stranded DNA; further mixing with a graphene oxide or reduced graphene oxide dispersion; and vacuum filtration to form the membrane. This membrane exhibits a high separation factor for chiral enantiomers such as L / D-phenylalanine, with an excess value of up to 99.95% for L-phenylalanine enantiomers after multi-stage separation, making it suitable for the efficient separation of chiral drugs and amino acids.

A method for characterizing a mobile particle in a sample (10), the method comprising: acquiring at least one image () of the sample during an acquisition period, using an image sensor (20) and forming a series of images, the series of images comprising at least one image; using each image of the series of images resulting from a) as an input image () of a convolutional neural network for detection (CNNd), the convolutional neural network for detection being configured to detect the particles and to produce, from each image, an output image () on which each detected particle is assigned an intensity distribution, centered on the particle and extending around the particle; for each detected particle, from each output image () resulting from b), estimating a position of each detected particle in each image of the series of images;characterization of each detected particle from the estimate of the position resulting from c), established from each image of the series of images.;

1. Name of the product in this design: Sperm Quality Analyzer. 2. Purpose of this design: The product is intended for use by research institutions and medical institutions for sperm concentration detection, motility analysis, sperm morphology identification, and determination of semen pH and DNA fragmentation rate in semen samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D rendering 2.

A method of artificial-intelligence-based robotic pipetting for spermatozoa preparation includes positioning a vessel containing a semen sample within a staging mechanism. The method includes using a robotic pipettor to make at least two droplets within a dish on the staging mechanism. The method includes using the robotic pipettor to connect the at least two droplets with a medium channel according to a programmable design commanded by an artificial intelligence / machine learning system (AI / ML system). The method includes using the robotic pipettor to deposit a quantity of sperm from the semen sample into one of the droplets. The method includes using the AI / ML system to optically scan the quantity of sperm to produce first and second image objects using an imaging system that includes a microscopy system, a camera system, and a lighting system. Creation of the image objects is separated by a specified time duration.

The invention discloses a yak frozen semen freezing process intelligent control method based on dynamic cooling curve optimization, and relates to the field of animal frozen semen preservation, and the yak frozen semen freezing process intelligent control method comprises the following steps: arranging a plurality of frozen semen tubes filled with consumed cattle semen on an annular tray with a cold source in the center in a radioactive manner, and uniformly arranging the plurality of frozen semen tubes in a surrounding manner in each radial distance interval; in the freezing process, cold energy is output according to a preset cooling program, and the annular tray is driven to rotate; unfreezing operation is executed, sperm motility values are measured, interval motility values of the sperm motility values of the frozen sperm tubules in all the radial distance intervals are counted, a temperature curve at the radial distance interval with the highest interval motility value is determined, a cooling program is modified in multiple rounds of experiments, and the process is repeated, so that the temperature curve is determined. And clustering the obtained temperature curves and selecting an optimal target temperature curve to perform freezing control. According to the method, the problem that the optimal dynamic cooling curve is difficult to efficiently obtain in the freezing process of the frozen yak sperms is solved.

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to the sperm acrosome intact rate of fresh sperm of a local breeding rabbit in Fujian and application of the SNP molecular marker. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID No: 1; the 94th basic group from the 5'end in the nucleotide sequence of the SNP molecular marker is represented by M, M represents A or G. The locus is related to the sperm acrosome integrity rate of the fresh sperm of the local breeding rabbit in Fujian. The fresh semen sperm acrosome intact rate of the local breeding Fujian rabbits with the genotype of homozygous GG at the site is obviously lower than that of the local breeding Fujian rabbits with the genotype of homozygous AA and heterozygous AG at the site. The SNP molecular marker disclosed by the invention is closely related to the sperm acrosome intact rate of the fresh semen of the local breeding rabbits of Fujian, and can be effectively used for molecular marker-assisted breeding of the local breeding rabbits of Fujian. The established detection method can be used for rapidly, efficiently and accurately detecting the sperm acrosome integrity rate of the fresh semen of the Fujian local breeding rabbits.

The invention belongs to the technical field of biology, and particularly relates to an acrossocheilus fasciatus sperm cryopreservation method which comprises the following steps: constructing a simulated water solution for acrossocheilus fasciatus in-vitro fertilization, collecting fresh sperms, detecting the survival rate, preparing a mixed solution of the sperms and anti-freezing components, sub-packaging, cooling and freezing and the like. According to the method, simulated water quality for in-vitro fertilization of acrossocheilus longifin is constructed, the survival rate of sperms observed and detected in the environment is closer to the fertilization condition during thawing activation in the future, and then the sperms with the higher fertilization success rate after thawing are screened out; meanwhile, the constructed simulated water solution can be used as a part of a cryopreservation solution, so that the remaining anti-freezing components can be directly added into the screened sperms, a mixed solution of the sperms and the anti-freezing components can be rapidly prepared under the condition that the service life of the sperms is not remarkably shortened, and the mixed solution is subpackaged, cooled and frozen; the improved anti-freezing component contains trehalose, soybean phospholipid and tea polyphenol, so that the survival rate and activity of the acrossocheilus fasciatus sperms after cryopreservation can be effectively improved.

The present invention relates to the identification and / or selection of sperm, and in particular a means for identifying and / or selecting the fittest sperm for use in assisted reproductive technologies.

The invention discloses a preparation for improving male erectile function and sperm quality. The health-care food is characterized by being prepared from the following raw materials in parts by weight: 30-50 parts of herba epimedii extract, 20-40 parts of herba cistanche extract, 15-30 parts of fructus lycii extract, 18-35 parts of semen cuscutae extract, 10-25 parts of herba cynomorii extract, 8-20 parts of maca powder, 6-18 parts of raspberry extract, 0.5-2 parts of lycopene, 1-3 parts of L-carnitine and 0.3-1 part of vitamin B family (vitamin B1, vitamin B2, vitamin B6 and vitamin B12 are mixed according to the mass ratio of 1: 1: 2: 0.5). The herba epimedii extract is selected as a main component, the content of icariin is regulated to be not lower than 60%, the high-content icariin can more effectively promote sex hormone secretion, enhance sexual desire and improve erectile function, the total content of echinacoside and verbascoside in the herba cistanche extract is not lower than 45%, and the herba epimedii extract and the herba cistanche extract have the effect of promoting sex hormone secretion, enhancing sexual desire and improving erectile function. The two components have the effects of tonifying kidney yang and benefiting essence and blood, and can enhance kidney functions.

The application relates to a sperm posture and motion state control method. The control method comprises the following steps: S1: using two laser beams to act on the head of a sperm; S2: adjusting the positions and powers of the two laser beams acting on the head of the sperm; the sperm can be controlled at a selected position, and the posture (referring to the relative position and angle between the head of the sperm and an imaging focal plane) and motion state of the sperm are controlled to obtain images of sperms in different directions and different motion states, wherein the posture and motion state of the head of the sperm and the relative focal plane of the sperm tail are included. The sperm relative to the imaging plane is controlled in the posture and motion state by adjusting the positions and powers of the two laser beams acting on the head of the sperm; for example, the motion of the sperm tail in a plane parallel to the imaging focal plane is controlled by controlling the posture of the sperm, and the motion images of the head of the sperm and the tail in a three-dimensional space are observed and obtained for a long time.

The invention provides an SNP (Single Nucleotide Polymorphism) marker for influencing the rapid forward movement sperm proportion of a pig. The sequence of nucleic acid of the SNP marker is shown as SEQ ID No.1, and the site of the SNP marker is located at the 301st site from the 5'end on the nucleic acid shown as SEQ ID No.1, corresponds to the 3803902 site from the 5 'end on the NW018084979.1 chromosome of the international pig genome of the version 11.1, and is C or T.

The present invention relates to a method for analysing a spermatozoon, comprising a step of extracting the nucleic acids from the spermatozoa contained in a first sample of spermatozoa previously obtained from a human subject; a step of measuring the level of expression of at least one marker gene chosen from the group consisting of AURKA, CFAP46, CCDC60, CCDC88B, HDAC4, CACNA1C, CACNA1H, CARHSP1, DNAH2 and SPATA18 from the extracted nucleic acids; and a step of determining the existence of an expression differential of the at least one marker gene in relation to a control. It also relates to a method for selecting spermatozoa and a method for evaluating the quality of a sperm.

The invention relates to a protective liquid formula for improving the survival rate of frozen and thawed sperms of pigs. The protective liquid formula comprises the following components in percentage by mass: 14% of sodium hexadecylsulfonate, 7% of coconut oil monoethanolamide and 79% of distilled water. The preparation method comprises the following steps: 1) weighing 14g of sodium hexadecylsulfonate and 7g of coconut oil monoethanolamide, and dissolving in 79g of distilled water; and 2) uniformly stirring the solution obtained in the step 1) at 60 DEG C until the sodium hexadecyl sulfonate and the coconut oil monoethanolamide are completely dissolved in the distilled water. The protection liquid has the advantages that the protection liquid protects porcine sperm cells, provides a suitable living environment for porcine sperms, improves the oxidative stress resistance and the preservation effect of the porcine sperms, ensures the survival rate of the porcine sperms, and further improves the breeding rate and the breeding benefits of sows; the survival rate, the sperm motility and the acrosome integrity rate of the frozen and thawed sperms of the pigs are obviously improved; the feed additive is simple in proportioning, high in reliability, low in economic cost, high in biological value and convenient to popularize and apply in production, and yolk with extremely high preparation requirements is not used.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, and an in vitro fertilization medium.

The invention relates to the technical field of lion-head goose breeding, in particular to a method for screening and identifying genes related to lion-head goose sperm motility regulation and control, which comprises the following steps: dividing healthy lion-head male geese in a breeding period into a high sperm motility group and a low sperm motility group according to sperm motility, collecting two groups of testicular tissues and sperm samples, and preserving the testicular tissues at low temperature for molecular detection; performing transcriptome sequencing on the testicular tissue to obtain mRNA and non-coding RNA expression data; screening two groups of nucleic acid molecules with significant differential expression, and determining candidate genes through functional enrichment analysis of reproductive development related biological pathways; and verifying the authenticity of the expression difference of the candidate gene through fluorescent quantitative PCR, and finally determining the target gene. According to the method, related data are obtained through sequencing, the candidate genes are determined by screening differential molecules through enrichment analysis and PCR verification, accurate identification of a related gene system is achieved, and the problems of missing detection and insufficient accuracy caused by lack of transcriptome sequencing in a traditional method are solved.

The purpose of the present invention is to provide spermatozoa having high motility. The present invention is a composition for treating sperm that comprises a mesenchymal stem cell culture supernatant. The composition for spermatozoa treatment of the present invention is used as a sperm preparing solution, a sperm diluting solution, a sperm storage solution, an artificial insemination solution, an in vitro fertilization solution, a solution for improving sperm motility, or a solution for retaining sperm fertilizing capabilities. Furthermore, mesenchymal stem cells in the mesenchymal stem cell culture supernatant comprised in the composition for treating sperm are preferably derived from adipose tissue, derived from umbilical cord tissue, or derived from bone marrow tissue.

The invention discloses a method for establishing an oligoasthenospermia model by using alpha-ketoglutaric acid. Alpha-ketoglutaric acid is added into drinking water of animals to obtain the oligoasthenospermia model. According to the invention, alpha-ketoglutarate (alpha-KG) is added into drinking water of a male mouse, so that the oligoasthenospermia mouse model is successfully established. The invention provides a new thought and clue for deeply researching the pathogenesis of oligoasthenospermia and researchers of male reproductive health, and has important scientific and medical values for understanding the mechanism of male infertility, developing a new therapy, evaluating the treatment effect and promoting related clinical research.

The present application relates to sperm movement technical field, especially a kind of method and device for measuring sperm head rotation frequency, comprising: S1, the image information of sample to be measured is obtained, based on the image information, select target sperm;S2, based on the target sperm, the light spot of the target sperm head after the target sperm is captured to head in the preset optical tweezers light beam is obtained;S3, based on the light spot of the target sperm head, the light spot intensity variation information is obtained, the light spot intensity variation information is converted into electrical signal data, the electrical signal data is carried out power spectrum analysis, and the rotation frequency of the target sperm head is obtained.The present application carries out real-time monitoring and analysis to sperm movement characteristics, can quickly obtain sperm movement characteristics, and the accuracy of measurement result is high.

The application provides a general single-stage sperm morphology image segmentation method and system, which comprises the following steps: obtaining a stained or unstained sperm microscopic image; inputting the sperm microscopic image into a single-stage segmentation network model, outputting a domain robust shared feature map through multi-view transformation and adaptive fusion; after multi-scale feature extraction and aggregation, a prediction head is used to output a semantic segmentation and / or instance-aware component segmentation result in parallel; tail skeleton features are extracted and topological optimization is performed based on a continuity constraint loss to maintain tail connectivity. The application enhances the cross-domain generalization capability by structure-appearance decoupling, and uses topological-aware learning to constrain the tail continuity, thereby solving the problems of weak generalization between stained and unstained images and tail segmentation fracture in high-density overlapping scenes in the prior art, and improving the clinical applicability and segmentation integrity.

The present application relates to the field of animal breeding technology, in particular to the application of ursolic acid in the frozen semen preservation of sheep. The present application applies ursolic acid in the frozen semen preservation of sheep. The addition of 0.5-2.0 micromole / L ursolic acid in the frozen diluent can improve the quality of frozen semen preservation of sheep and goats, and can improve the sperm motility and vitality after thawing, which helps the thawed sperm to pass through the vagina, uterus and enter the oviduct to complete fertilization, improves the insemination success rate of frozen sperm, and provides technical support for strengthening the protection of local sheep breed resources.

The present application relates to the technical field of image processing, and discloses a light staining sperm microscopic image light-weight style consistency preprocessing method and system, comprising: acquiring a light staining sperm microscopic image, performing color normalization on the light staining sperm microscopic image according to a deep staining sperm microscopic image, performing local contrast enhancement on the normalized light staining sperm microscopic image according to local contrast of a sperm head, a tail and a boundary region, performing brightness correction on the enhanced light staining sperm microscopic image according to the definition of the sperm head, the middle section and the tail structure and the background stability, and obtaining a light staining sperm microscopic image after light-weight style consistency preprocessing. The present application can solve the problems of staining difference, uneven brightness and boundary blur through image preprocessing without increasing the number of samples and additional training burden, improve the overall quality of the light staining sperm microscopic image, and improve the detection performance of the subsequent model.

The invention discloses a special biscuit for improving sperm fertilization ability of boars under heat stress and a processing method thereof, and the biscuit comprises the following components by weight: 10% of grease; the grease comprises DHA (docosahexaenoic acid) and EPA (eicosapentaenoic acid), and the weight ratios of the DHA and the EPA in the grease are respectively 30% and 35%; 8%-18% of an amino acid component; the weight ratio of lysine to methionine to tryptophan to threonine to arginine in the amino acid component is 100 to (26 to 38) to (18 to 32) to (70 to 90) to (60 to 120); the vitamin component comprises 0.6%-1.0% of vitamin A, 1%-3% of L-carnitine, 0.5%-1% of vitamin E and 0.5%-1% of vitamin D; the trace element components comprise 0.01%-0.1% of nano selenium, 0.1%-1% of nano zinc and 0.02% of organic chromium; and the balance of corn starch. The special biscuit can improve the sperm motility of the boars and improve the reproductive nutrition status of the boars, the special biscuit is designed into independent quantitative solid blocks, and the problems that powdery additives are inaccurate in metering, uneven in mixing and waste in throwing can be solved.

The invention relates to the technical field of animal feeds, in particular to a mouse feed and a preparation method and application thereof, and the mouse feed comprises curcumin and a basic ration; wherein the curcumin is provided in the form of a drug-loaded pellet; the basic ration is SPF (specific pathogen free) grade rat ration. The feed for the rats contains curcumin, effectively relieves damage of the plateau low-oxygen environment to the reproductive system of the rats, improves sperm motility, reduces the malformation rate, adjusts the reproductive hormone level, and provides an innovative solution for plateau animal reproductive health management.

Disclosed is a device for collecting motile sperm, the device comprising a post having a top, a bottom, and a wall, the wall comprising a mesh layer and a membrane layer, an inlet disposed at the top of the post, and a nose disposed at the bottom of the post.

The invention relates to the field of human reproductive health, in particular to a memristor for detecting whether sperms are contained in a seminiferous tubule or not and a preparation method and application of the memristor. The memristor comprises a bottom electrode layer, a buffer layer, an intermediate layer and an upper electrode layer from bottom to top, the bottom electrode layer is a conductive electrode layer, the buffer layer is MnO2, the intermediate layer contains carotene powder and PVDF powder, the upper electrode layer is a metal element electrode layer, and conductive metal elements provide migration metal ions under the action of an electric field. The memristor has a memristive effect, shows excellent stability under a certain condition, and can detect whether sperms are contained in seminiferous tubules or not. The memristor device with the memristive effect and the sensitive sensing function has a wide prospect in multi-scene application of electronic devices in the future.