231 results about "Spermatozoon" patented technology

The invention discloses a bull sperm motility related SNP molecular marker based on an HSPB9 gene, a detection product and application, and belongs to the technical field of molecular biology, the SNP molecular marker is located at the position of a bovine genome Chr19: 42255643, and the polymorphism is C or T. The research finds that the SNP genotype combination with high sperm motility of the bull is TT. By detecting the SNP of the SNP site, the sperm motility evaluation of the bull can be realized, the breeding is assisted, a reliable support is provided for breeding a high-quality next generation, and the method has the advantages of high reliability, high efficiency, low cost and the like.

The invention belongs to the technical field of sperm image segmentation detection, and particularly relates to a multi-sperm image segmentation detection system based on a yov8 framework. The multi-sperm image segmentation detection system is based on a yolov8 network, an HAT network and a RevColV1 network are fused, a multi-sperm segmentation model yolov8-HAT and a single-sperm detection model yolov8-RevColV1 are designed, and the two models are cascaded to form the multi-sperm image segmentation detection system; the YOLOv8-HAT model is obtained by fusing a hybrid attention converter (HAT) on the basis of a YOLOv8 network framework, and is used for segmenting a multi-sperm image; the yolov8-RevColV1 is formed by fusing a reversible column network (RevColV1) on the basis of a yolov8 network framework, and is used for detecting a single sperm; according to the method, the label corresponding to each sperm can be displayed on the multi-sperm image, the problem that it is difficult for a doctor to effectively count and judge whether the sperms are normal when the sperms in the multi-sperm image are dense is solved, and the sperm image segmentation efficiency is improved.

The present invention provides a method for accurately measuring live sperm morphology, including the following steps: S11. acquiring an image including a plurality of sperm cells; S12. detecting the sperm cells in the image, and segmenting a head, a midpiece, and a tail of each detected sperm cell; and S13. calculating morphological parameters of each sperm cell based on a segmentation result. For the method for accurately measuring live sperm morphology of the present invention, an image of sperm cells is acquired, a head, a midpiece, and a tail of each sperm cell are segmented through image processing, and morphological parameters of each sperm cell are calculated based on a segmentation result. Morphological parameters of live sperm cells can be comprehensively and accurately obtained for analysis or selection.

The invention provides a method for cultivating hybrid fries of Jinhu and tiger. The method comprises the following steps: selecting a breeding environment; breeding and ripening female parents; management and control before oviposition; insemination; hatching fertilized eggs; fry rearing. According to the method, in the female parent breeding and ripening stage, specific biological bait is controlled to be fed in stages, feeding of the parent fish can be promoted, growth can be accelerated, in cooperation with specific compound feed in the management and control stage before oviposition, favorable conditions are created for subsequent oviposition, and the quality and number of ova can be improved. Low-frequency ultrasonic assisted fertilization is introduced in the artificial insemination stage, so that the activity of ovum sperms can be obviously improved, and the fertilization rate is increased; in the fertilized egg hatching stage, intermittent ultrasound is assisted, the hatching rate can be increased, and the seedling yield of the golden-tiger hybrid spots can be increased; after hatching, transferring into a specific culture pond, slowly releasing beneficial bacteria, improving the adaptability of the fry, adsorbing harmful substances, reducing pathogenic bacteria, stabilizing water quality, promoting the growth of the fry, improving the survival rate and reducing the malformation rate.

The present invention provides an automatic semen analysis method based on multi-frame information fusion with occlusion awareness. The present invention first proposes the combination of an edge-sensitive U-Net model and an occlusion-aware tracker based on joint probabilistic data association to achieve accurate and stable sperm detection and tracking. Thanks to the pixel-level contour segmentation and the overlap inference based on multi-frame target contours of the present invention, the present invention can accurately distinguish two or more spermatozoa that are close but not overlapping, and provide accurate head positioning; can accurately predict and judge the occurrence, progress, and end of target overlap; and can effectively match spermatozoa before and after overlap to ensure the consistency of tracking. The present invention can be conveniently integrated into existing computer-aided sperm analysis systems to provide semen analysis data with better accuracy and robustness.

The invention relates to the technical field of sperm morphology detection, in particular to a sperm morphology training prediction method and system based on YOLO-OBB, and the method comprises the steps: collecting a sperm image, and carrying out the preprocessing of the sperm image, and obtaining a target image; frame selection is carried out on sperms in the target image by using a plurality of directional bounding boxes to obtain bounding boxes, sperm forms in the bounding boxes are labeled to obtain a labeled data set, and labeled labels are sperm counts, normal sperms, normal sperm heads, normal sperm middle segments and abnormal sperm main segments. According to the method, after the sperm part in the target image is selected through the OBB, the noise influence degree of the image in the bounding box can be calculated, and when the noise influence degree is larger than the preset threshold value, the image in the bounding box is deleted. The use of the images in the bounding box with noise influence to train the YOLO detection model is avoided, and the training efficiency and performance of the YOLO detection model are improved.

The invention provides a sperm automatic detection and tracking method, sperm automatic detection and tracking equipment and a storage medium. The method comprises the following steps: firstly, preprocessing a sperm image, performing rotation bounding box labeling on a head and a tail, and generating a training set containing a center point coordinate, a width, a height and an angle; training a target detection model by using the training set to obtain a model capable of automatically identifying sperms and outputting a rotating bounding box; detecting video frames by using the model, and extracting the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and track continuity, adjacent frame targets are matched, a comprehensive score is generated, the targets are associated, and a continuous track and a unique identifier are formed and used for calculating motion parameters; and judging whether the track is static or low active according to the speed and displacement of the latest N frames, sealing the track to eliminate subsequent matching, and only reserving a non-sealed track to calculate motion parameters. According to the method, interference is effectively avoided, and the tracking accuracy and the reliability of sperm motility parameter calculation are remarkably improved.

The invention discloses an application of luteolin in improving the reproductive performance of cocks. Researches show that in the breeding process of the cocks, the breeding performance of the cocks can be effectively improved by feeding the luteolin. Test data show that the luteolin can significantly improve the cock semen collection amount, improve the sperm density and sperm motility, significantly improve the diameter of a fine sperm tube and a spermatogenic cell matrix layer, and artificial insemination of the test cock semen improves the hatching egg fertilization rate, the hatching rate and the healthy chick rate. Therefore, the luteolin has important significance in research and development of related products for improving the reproductive performance of the cocks, and the luteolin has great potential in preparation of medicines, feeds or feed additives for improving the reproductive performance of the cocks.

According to the Hainan pig semen preservation diluent as well as the preparation method, the application and the detection method thereof disclosed by the invention, galangin and kaempferide are added into a basic diluent to develop a diluent formula capable of efficiently preserving semen at a low temperature (4 DEG C); the galangin and the kaempferol added into the diluent have a good sperm protection effect and a certain antibacterial ability and can replace antibiotics, and bovine serum albumin (BSA) is further combined as a low-temperature protective agent, so that sperm movement parameters are improved, and the low-temperature preservation time of the boar semen is effectively prolonged; the use amount of antibiotics in pig raising production is reduced, the problem of drug resistance of bacteria can be effectively relieved, and the use of the antibiotics and the generation of drug-resistant bacteria are greatly reduced. According to the method, the semen utilization rate of excellent breeding boars is increased, sow health is guaranteed, the conception rate of deep insemination is increased, and efficient and low-cost artificial insemination is achieved.

Systems for sorting sperm include a housing and a microfluidic system supported by the housing. The systems also include two or more inlets providing access to the microfluidic system to deliver sperm or semen and fluid to the microfluidic system, as well as an outlet for harvesting sorted sperm. The systems include a flow channel that provides a flow path for sperm from an inlet to an outlet while sperm travels against a fluid flow towards an outlet for harvesting. In the systems, fluid delivered to the microfluidic system via an inlet flows from the inlet towards one or more outlets, and the flow channel extending along the length of the microfluidic system provides a flow path for motile sperm to travel against the fluid flow towards a collection outlet for harvesting. In some embodiments of a system, the microfluidic system also includes a microporous filter arranged in the flow path between the inlet and the collection outlet to cause motile sperm traveling against the fluid flow to pass through the filter to reach the collection outlet for harvesting.

The invention discloses a feed for improving the quality of semen of an old boar and an application of the feed. The feed disclosed by the invention is prepared from the following components in parts by weight: 8 to 10 parts of corn, 18 to 23 parts of red sorghum, 37 to 43 parts of hulled barley, 12 to 17 parts of bran, 0.5 to 1.5 parts of functional fiber, 7 to 9 parts of soybean meal, 1.5 to 2.5 parts of fish oil or microalgae powder, 1.3 to 1.4 parts of mountain flour, 1 to 1.2 parts of monocalcium phosphate, 0.4 to 0.5 part of sodium chloride, 0.2 to 0.3 part of lysine, 0.1 to 0.15 part of L-threonine, 0.1 to 0.15 part of choline chloride, 1 to 1.5 parts of betaine and 0.5 to 1 part of a premix for boars. After the feed is applied, the total effective sperm number of the aged boars is remarkably increased, the sperm motility and form are improved, the breeding age limit is prolonged, the production value is increased, and the biological safety risk caused by the high renewal rate is reduced.

The invention provides a recombinant lentivirus capable of indicating bovine spermatogonial stem cell differentiation, a spermatogonial stem cell line and application, and belongs to the technical field of cell biology. The invention provides a method for constructing a spermatogonial stem cell line for indicating bovine sperm differentiation on the basis of a lentivirus transduction technology, and a visual detection system for differentiating spermatogonial stem cells into spermatoblasts is constructed by regulating expression of mCherry protein by utilizing a PRM1 promoter. According to the method, the expression of PRM1 is indicated by fluorescent protein mCherry, so that the differentiation process of the bovine spermatogonial stem cells is indicated, and a good tool is provided for the induction of differentiation of the bovine spermatogonial stem cells to sperms, the research of a development mechanism and the optimization of a differentiation system of the bovine spermatogonial stem cells. The method is a new way for solving the neck clamping problem that good-variety bovine sperms are difficult to obtain in scientific research and production, can also be used for optimization of a spermatogonial stem cell differentiation system, sorting and purification of sperms, even downstream research of drug screening and the like, and has a good application prospect.

A discrimination system with optics that collect output electromagnetic radiation from sperm cells within a measurement region, a split detector that generates an electrical signal responsive to the intensity of the collected electromagnetic radiation and which detects the position of sperm cells in the measurement region, and an analyzer for discriminating between X-chromosome bearing sperm and Y-chromosome bearing sperm in the measurement region.

Provided in the present utility model is an inner container opening structure of a sperm extractor, including: a soft rubber inner container configured to massage a male genital organ; and a power mechanism mounted on the soft rubber inner container and configured to generate a vibration, where the soft rubber inner container includes an opening portion and a main body portion; the main body portion is mounted at the opening portion, and a channel is formed inside the opening portion and the main body portion; the power mechanism is disposed at the opening portion; a contact plate is disposed at a lower end of the opening portion; the power mechanism is mounted on the contact plate; and two arc-shaped grooves are further formed in the contact plate.

The application provides a sperm automatic detection and tracking method, device and storage medium. The method first pre-processes a sperm image, performs head-tail rotation boundary box labeling, and generates a training set containing center point coordinates, width, height and angle; a target detection model is trained using the training set to obtain a model capable of automatically identifying sperm and outputting a rotation boundary box; the model is applied to detect video frames to extract the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and trajectory continuity to match adjacent frames of targets, generate a comprehensive score and associate the targets to form a continuous trajectory and a unique identifier for calculating motion parameters; and then whether the trajectory is stationary or low active is judged according to the speed and displacement of the last N frames, the trajectory is sealed to exclude subsequent matching, and only the non-sealed trajectory is retained to calculate the motion parameters. The method effectively avoids interference, significantly improves the tracking accuracy and the reliability of sperm motility parameter calculation.

The invention discloses a micro-fluidic chip for exploring in-vitro XY type sperm fertilization preference, and relates to the technical field of biomedical engineering.According to the technical scheme, the micro-fluidic chip is characterized by comprising a chip body, the chip body is provided with a central sample carrying hole, the central sample carrying hole is used for containing an oocyte, and the central sample carrying hole is communicated with the central sample carrying hole; the chip body is provided with four peripheral sample carrying holes, the four peripheral sample carrying holes are used for placing fluorescently-labeled sperm cells subjected to sexual control sorting, and communicated micro-channels are arranged between the central sample carrying hole and the four peripheral sample carrying holes. Observation is facilitated by improving the volume of a liquid carrying hole and optimizing the transparency of a material, meanwhile, by adjusting the diameter of a channel and the number of repeated units, the thickness of the micro-fluidic chip is reduced, micro-channel blockage is avoided, the number of cells in loaded liquid is guaranteed, and the detection accuracy of the micro-fluidic chip is improved. The preference of successful fertilization of X and Y type sperms under different conditions is observed in vitro through micro-fluidic, and a new thought is provided for a method for controlling the gender of a fetus.

The invention discloses ultralow-temperature preservation liquid for Sinilabeo decorus tungting sperms as well as a preparation method, a preservation method and application of the ultralow-temperature preservation liquid and belongs to the technical field of artificial breeding of Sinilabeo decorus tungting. The ultralow-temperature preservation liquid comprises a freezing diluent, an antifreeze agent glycol and an antioxidant, the antifreeze agent accounts for 8% of the volume of the ultralow-temperature preservation liquid, the antioxidant comprises reduced glutathione and alpha-lipoic acid, and the balance is the freezing diluent. According to the present invention, the formula compounding is different from the simple superposition of the traditional formula, the ratio and the combination mode of the components are precisely optimized, the combination has the synergistic effect, the resuscitation sperm activity is improved, the good oxidation resistance is provided, and the effect far better than the effect of other schemes is achieved. The method has remarkable advantages in efficient preservation of the sinilabeo decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus

An automated method for assessing the ZP binding capacity of sperm from morphological characteristics of sperm in assisted reproduction using deep learning is disclosed. The invention also provides a method for predicting fertilization success based on ZP binding capacity of sperm in clinical assisted reproduction.

This invention belongs to the field of membrane separation and functional membrane materials technology, and discloses a chiral graphene oxide-based separation membrane, its preparation method, and its application. The membrane is composed of graphene oxide and / or reduced graphene oxide sheets and a single-stranded DNA-single-walled carbon nanotube complex. The single-stranded DNA helically winds around the outer surface of the single-walled carbon nanotubes to form a one-dimensional chiral nanochannel and intercalates between the sheets, forming a three-dimensional chiral channel network connected in the membrane thickness direction. The preparation method includes: heating and decohexing salmon sperm double-stranded DNA in a urea-containing buffer to obtain single-stranded DNA; dispersing single-walled carbon nanotubes and then complexing them with the single-stranded DNA; further mixing with a graphene oxide or reduced graphene oxide dispersion; and vacuum filtration to form the membrane. This membrane exhibits a high separation factor for chiral enantiomers such as L / D-phenylalanine, with an excess value of up to 99.95% for L-phenylalanine enantiomers after multi-stage separation, making it suitable for the efficient separation of chiral drugs and amino acids.

The present invention relates to the field of genetic breeding technology, and specifically discloses a SNP molecular marker, primer, and application related to linear motility of sperm after cryopreservation and thawing of chicken semen. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO.1, and the 53rd position in the SNP molecular marker is a SNP site, which has a G / A polymorphism. The SNP molecular marker provided by the present invention has a SNP site at position 53, which has a G / A polymorphism. The proportion of linearly motile sperm in semen of individuals with a GA genotype after cryopreservation and thawing of semen is higher than the proportion of linearly motile sperm in semen of individuals with an AA genotype after cryopreservation and thawing of semen.

A method for characterizing a mobile particle in a sample (10), the method comprising: acquiring at least one image () of the sample during an acquisition period, using an image sensor (20) and forming a series of images, the series of images comprising at least one image; using each image of the series of images resulting from a) as an input image () of a convolutional neural network for detection (CNNd), the convolutional neural network for detection being configured to detect the particles and to produce, from each image, an output image () on which each detected particle is assigned an intensity distribution, centered on the particle and extending around the particle; for each detected particle, from each output image () resulting from b), estimating a position of each detected particle in each image of the series of images;characterization of each detected particle from the estimate of the position resulting from c), established from each image of the series of images.;

1. Name of the product in this design: Sperm Quality Analyzer. 2. Purpose of this design: The product is intended for use by research institutions and medical institutions for sperm concentration detection, motility analysis, sperm morphology identification, and determination of semen pH and DNA fragmentation rate in semen samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D rendering 2.

A method of artificial-intelligence-based robotic pipetting for spermatozoa preparation includes positioning a vessel containing a semen sample within a staging mechanism. The method includes using a robotic pipettor to make at least two droplets within a dish on the staging mechanism. The method includes using the robotic pipettor to connect the at least two droplets with a medium channel according to a programmable design commanded by an artificial intelligence / machine learning system (AI / ML system). The method includes using the robotic pipettor to deposit a quantity of sperm from the semen sample into one of the droplets. The method includes using the AI / ML system to optically scan the quantity of sperm to produce first and second image objects using an imaging system that includes a microscopy system, a camera system, and a lighting system. Creation of the image objects is separated by a specified time duration.

The invention discloses a yak frozen semen freezing process intelligent control method based on dynamic cooling curve optimization, and relates to the field of animal frozen semen preservation, and the yak frozen semen freezing process intelligent control method comprises the following steps: arranging a plurality of frozen semen tubes filled with consumed cattle semen on an annular tray with a cold source in the center in a radioactive manner, and uniformly arranging the plurality of frozen semen tubes in a surrounding manner in each radial distance interval; in the freezing process, cold energy is output according to a preset cooling program, and the annular tray is driven to rotate; unfreezing operation is executed, sperm motility values are measured, interval motility values of the sperm motility values of the frozen sperm tubules in all the radial distance intervals are counted, a temperature curve at the radial distance interval with the highest interval motility value is determined, a cooling program is modified in multiple rounds of experiments, and the process is repeated, so that the temperature curve is determined. And clustering the obtained temperature curves and selecting an optimal target temperature curve to perform freezing control. According to the method, the problem that the optimal dynamic cooling curve is difficult to efficiently obtain in the freezing process of the frozen yak sperms is solved.

The invention belongs to the technical field of medicines, and discloses a gastrodia elata microbial fermentation product as well as a preparation method and application thereof. According to the invention, three aspergillus oryzae are utilized to successfully carry out microbial fermentation on gastrodia elata, the gastrodia elata microbial fermentation product of which the content of active ingredients gastrodin and p-hydroxybenzyl alcohol is obviously increased is obtained, and the pharmacological action of the gastrodia elata microbial fermentation product on treating or improving oligoasthenospermia is verified through an oligoasthenospermia mouse model; results show that the gastrodia elata microbial fermentation product has better effects of improving sperm concentration, enhancing sperm motility and rapidly moving forwards, can be used as an active ingredient of a medicine for treating or improving oligospermia and asthenospermia, and has wide application.

The invention discloses a universal production process for human and animal sperm slides, comprising a conveying device, on the periphery of which are sequentially mounted a negative film loading device, a glue screen printing device, a cover film loading device, a transfer device, a height limiting pressing device and a slide unloading device; the full-process production of the slides is achieved through the conveying device, the negative film loading device, the glue screen printing device, the cover film loading device, the transfer device, the pressing device and the slide unloading device; in addition, through the adjustment of a high-precision servo motor, a screw pair and a micrometer, the production of slides of different chambers can be achieved as required, the applicability is strong, and the production efficiency is effectively improved.

The invention discloses an application of a carrageenan degradation product in preparation of a medicine for improving male reproductive power. According to the method disclosed by the invention, a compound capable of improving sperm motility and male reproductive power is obtained, and a new thought and clue are provided for deeply researching the pathogenesis of oligoasthenospermia and researchers of male reproductive health in the future.

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to the sperm acrosome intact rate of fresh sperm of a local breeding rabbit in Fujian and application of the SNP molecular marker. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID No: 1; the 94th basic group from the 5'end in the nucleotide sequence of the SNP molecular marker is represented by M, M represents A or G. The locus is related to the sperm acrosome integrity rate of the fresh sperm of the local breeding rabbit in Fujian. The fresh semen sperm acrosome intact rate of the local breeding Fujian rabbits with the genotype of homozygous GG at the site is obviously lower than that of the local breeding Fujian rabbits with the genotype of homozygous AA and heterozygous AG at the site. The SNP molecular marker disclosed by the invention is closely related to the sperm acrosome intact rate of the fresh semen of the local breeding rabbits of Fujian, and can be effectively used for molecular marker-assisted breeding of the local breeding rabbits of Fujian. The established detection method can be used for rapidly, efficiently and accurately detecting the sperm acrosome integrity rate of the fresh semen of the Fujian local breeding rabbits.

The invention discloses an application of gallnut tannic acid as an additive in boar production. The application of the gallnut tannic acid as the additive comprises the application of the gallnut tannic acid as a feed additive in improving the reproductive performance of adult breeding boars and the application of the gallnut tannic acid as a semen diluent additive in improving the normal-temperature preservation quality of boar semen. The feed additive can improve the reproductive performance of adult breeding boars, can significantly improve the sperm density, the total sperm number and the effective sperm number of the boars after being fed for 12 weeks, and improves the oxidation resistance of sperms, sperms and serum; the semen diluent additive can improve the normal-temperature preservation quality of the boar semen, and the sperm motility can still reach 61.20% after the boar semen is preserved for 7 days.