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275 results about "Spermatozoon" patented technology

A spermatozoon (pronounced /ˌspɜːrmætəˈzoʊən/, alternate spelling spermatozoön; plural spermatozoa; from Ancient Greek: σπέρμα ("seed") and Ancient Greek: ζῷον ("living being")) is a motile sperm cell, or moving form of the haploid cell that is the male gamete. A spermatozoon joins an ovum to form a zygote. (A zygote is a single cell, with a complete set of chromosomes, that normally develops into an embryo.)

System of hysteroscopic insemination of mares

InactiveUS20040031071A1Low total numberMaintenance success rateAnimal reproductionGenetic engineeringBiologyEpididymis
Abstract of the Disclosure The present invention provides a method of producing a mammal through artificial insemination and is directed, in particular embodiments, to low spermatozoa numbers for insemination and the production of a mammal through the use of hysteroscopic insemination techniques. The present invention is particularly directed to embodiments potentially regarding fresh or preserved sperm, treated or processed sperm, sperm inserted under a surface in the vicinity of the uterotubal junction, hysteroscopic compatible media for the establishment of the insemination sample, hysteroscopic compatible volume for insemination, epididymal use of the hysteroscopic technique, bubble or froth insemination utilized in hysteroscopic insemination, and for sorted and frozen sperm utilized in hysteroscopic insemination. The disclosed embodiments may be directed at a mammal species, particularly equids, bovids, and swine, as well as animals produced in accordance with any of the disclosed embodiments of the present invention.
Owner:XY

Integrated herd management system utilizing isolated populations of X-chromosome bearing and Y-chromosome bearing spermatozoa

Animal management systems that utilizes artificial insemination (27) samples of isolated X-chromosome bearing and Y-chromosome bearing populations of spermatozoa to inseminate female animals (17) an embodiment of which can provide an alternative to traditional harvesting or marketing (20) of non-replacement heifers.
Owner:XY

System for in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comrprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Owner:XY

System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations

Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformity stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.
Owner:XY

In-Vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Owner:XY

Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe

The invention relates to a diluent of frozen semen of a milk goat, and a preparation method and a diluting method thereof as well as a preparation method of frozen semen of the milk goat in a thin pipe. In an existing frozen semen storage method, sperms are struck by low temperature to cause structure change of plasmalemma, so that the integrity of the plasmalemma and the survival rate and the fertility of the sperms after resuscitation are seriously influenced. The preparation method is characterized in that thin-pipe freezing treatment with two-step dilution and balancing and two-step cooling is carried out on fresh semen, wherein a basic diluent is prepared from Tris, citric acid, glucose, trehalose, Vc, GSH, BSA, ATP, compound antibiotic, yolk and water; a frozen diluent is prepared from the basic diluent and glycerinum; the diluent is utilized for diluting and balancing the fresh semen, and the fresh semen is sub-packaged in thin pipes and is stored in liquid nitrogen. According to the invention, the effects of low-temperature storage and freezing storage of the frozen semen of the milk goat is good, the survival rate, the integration rate of acrosome and the integrity of the plasmalemma of the semen after unfreezing are effectively increased, the aberration rate is reduced and the production efficiency and the quality of the frozen semen of the milk goat are improved.
Owner:NORTHWEST A & F UNIV

Freezing method of pig sperms

InactiveCN102138552AImprove integrityImprove in vitro fertilization (IVF) ratesDead animal preservationAnimal scienceLow density
The invention discloses a freezing method of pig sperms. Unexpectedly, the DNA (deoxyribonucleic acid) completeness and the in vitro fertilization (IVF) rate of unfrozen pig sperms can be obviously improved by using 9(w / v)% of low-density albumin for treatment during the freezing process.
Owner:金一

In vitro fertilisation

The present invention relates to a method and a system for producing a mammalian pre-embryo and a stem cell having a better quality than prior art methods. The system comprises means for obtaining a mammalian oocyte, and means for obtaining a mammalian spermatozoa, and an apparatus having at least two separate air-tight chambers, for which the oxygen tension of one chamber may be changed independent of the oxygen tension of the other chamber, said at least two separate air-tight chambers constitute a main chamber and at least one residence chamber. The method for in vitro producing a mammalian pre-embryo comprising the steps: a1) providing a mammalian oocyte, a2) providing a mammalian spermatozoa, b) culturing the oocyte and the spermatozoa, c) fertilizing the oocyte with the spermatozoa obtaining a fertilized oocyte, and d) allowing cell-division of the fertilized oocyte obtaining a multicellular pre-embryo wherein at least one of the steps a1) or a2) is conducted at an oxygen tension below 15%, or e) allowing cell-division of the fertilized oocyte obtaining a multicellular pre-embryo, wherein the culture is performed at an oxygen tension allowing cultivation of the cells and wherein at least one of the steps comprises a change in the oxygen tension. Stem cells are produced from the multicellular pre-embryo.
Owner:REGION HOVEDSTADEN VHERLEV HOSPITAL

Method for inducing cleavage gynogenesis of fish fry of cynoglossus semilaevis

The invention discloses a method for inducing cleavage gynogenesis of fish fry of cynoglossus semilaevis, which comprises the steps of cynoglossus semilaevis sperm freezing storage, ultraviolet radiation of sperms, and induction and identification of the cleavage gynogenesis of the fish fry. The cynoglossus semilaevis sperm freezing storage method is established, and a method for inhibiting the first cleavage to induce the multiplication of embryo chromosome of the gynogenesis of the cynoglossus semilaevis through hydrostatic pressure is adopted so as to screen effective dilution and freezing method for the cynoglossus semilaevis sperm freezing storage and screen the hydrostatic pressure shock starting time, hydrostatic pressure and processing time suitable for the cleavage gynogenesis of cynoglossus semilaevis; and an identification method for the gynogenesis of the fish fry of the cynoglossus semilaevis is established. In the method, the fish fry of the cynoglossus semilaevis through cleavage gynogenesis is obtained for the first time, and the inductivity of the gynogenesis of the fish fry reaches 0.25 percent; and the method has the characteristics of advancement, high efficiency, reliability and reliability, can be used for solving the problems that male cynoglossus semilaevis grows slowly, the ratio of female cynoglossus semilaevis is low and the culture cost is high, and has significant application value and wide promotion and application prospect in the aspects of breeding of cynoglossus semilaevis fry, sex control, culture of all female fry, pure line breeding and the like.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Hybrid breeding method for sea cucumber

A hybridization method between Chinese spiny sea cucumber and Russian (or korean) one for preparing the hybrid one with high growth speed, stress resistance, survival rate and nutritive value includes such steps as using special culture technique to make them mature symultaneously, artificial oxytocia to obtain their spermatozoa and ova, artificial fertilization, and culturing fertilizer ova until they become laval plankton.
Owner:DALIAN FISHERIES UNIVERSITY

Microinjection assembly and methods for microinjecting and reimplanting avian eggs

The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.
Owner:AVIGENICS

Porcine NTF3 promoter region SNP as molecular marker for boar breeding traits and applications of porcine NTF3 promoter region SNP

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A / G and G / A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.
Owner:HUAZHONG AGRI UNIV

Sperm acrosome zone Raman spectrum peak and use thereof

The invention relates to a sperm acrosome zone Raman spectrum peak and a use thereof. The Raman spectrum peak is represented by an abstract figure shown in the specification, and can be used for the fertilization function assessment and detection and the theory researches of a sperm. The invention also relates to a sperm fertilization function assessment method. The method comprises the following steps: 1, preparing a spermatic slice; 2, scanning the single sperm acrosome zone on the spermatic slice at a laser excitation wave of 532nm under a laser power of 5mW three times to obtain a Raman data spectrum peak; and 3, contrasting the Raman data spectrum peak of a detection sample with the Raman data spectrum peak of the above sperm acrosome zone to determine that whether the sperm has a fertilization function or not. The method provides a standard Raman spectrum peak for determining the sperm fertilization potential, and the determination result of the method is accurate and reliable, and has a high credibility, so the method is a new sperm fertilization potential detection method; and a separate sperm can be controlled by the method, so there is no need to specially process the sperm, the damage of the sperm can controlled in an extremely low range, and the sperm can be directly used for subsequent researches or auxiliary reproduction treatment.
Owner:RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Culture solution for bovine in vitro fertilization (IVF) and method for improving bovine IVF

The invention relates to a culture solution for bovine IVF and a method for improving bovine IVF. The method for bovine IVF comprises the following steps: washing a mature cumulus-oocyte complex in afertilization culture solution, transferring the mature cumulus-oocyte complex into the fertilization culture solution, and putting the fertilization culture solution into an incubator for later use;taking a frozen seminal tubule from liquid nitrogen, performing unfreezing in a water bath at 37 DEG C, injecting seminal fluid into a centrifuge tube containing a seminal fluid preparation culture solution, and discarding a supernatant after centrifugation; adding the seminal fluid preparation culture solution into the centrifuge tube, resuspending sperm precipitate, and taking a proper sperm suspension for sperm counting; and adding the sperm suspension with the calculated volume into fertilization culture liquid drops containing oocytes, placing a culture tray into the incubator, and carrying out sperm-ovum co-incubation to complete IVF operation for embryo in-vitro culture. The fertilization culture solution and the seminal fluid preparation culture solution contain sodium pyruvate, heparin and other components. The method can significantly improve the efficiency of bovine IVF.
Owner:天津力牧生物科技有限公司

Two-layer percoll density gradient centrifugal separation method of boar sperms

InactiveCN102250836ASimple processing methodEasy to handleGerm cellsAnti-sperm antibodySemen liquefaction
The invention provides a two-layer percoll density gradient centrifugal separation method of boar sperms. The method is suitable for normal sperm specimen and sperm specimen with low sperm density or low sperm motility, and is a simple, convenient and fast sperm treatment method. The method can be used for remove leucocytes, bacteria, fragments and anti-sperm antibodies in the sperms and improving the delayed sperm liquefaction, the sperm in-vitro capacitation and the like, so that the sperms can effectively fertilize ova to improve the pregnancy rate, and the percentage of the sperms with motility and normal shape in the obtained sperms is about 95%. The method provided by the invention is suitable for screening sperms with motility from fresh boar sperms and diluted sperms, thus providing high-quality sperms for the study of boar sperm capacitation mechanism and in-vitro fertilization.
Owner:JILIN UNIV

In vitro human sperm culture solution for improving sperm motility and application thereof

The invention discloses in vitro human sperm culture solution for improving sperm motility and application thereof. The culture solution contains fructose 6-phosphoric acid (F6P). The in vitro human sperm culture solution for improving the sperm motility of human sperms is used for culturing the purified human sperms which leave from a human body; the vitality of normal human sperms is capable of maintaining sperm movement and can be obviously improved; and a meaningful reference is provided for improving and assisting the reproductive technology. The F6P is directly used for cultivating and diluting the culture solution, and does not relate to organic solvents such as ethanol or dimethylsulfoxide (DMSO) and the like; the toxic action of the ethanol or the DMSO on the sperms is avoided; by adopting the F6P added to the sperm culture solution, the vitality of the sperms of clinical patients with asthenospermia can be obviously improved; the improvement degree of the vitality is the most obvious for severe oligospermia; the activity of a part of cultured sperms reaches the normal sperm vitality range; and the improvement degree of the vitality for the severe oligospermia is the most obvious. This fully demonstrates that low motility of the sperms of the patients with asthenospermia can be improved by using of the F6P.
Owner:NANJING MEDICAL UNIV

Method for generating macrogametocyte by somatic cell of inducing undaria pinnatifida gynecogenic juvenile sporophyte

The invention discloses a method for generating macrogametocytes by somatic cells of inducing undaria pinnatifida parthenogenesis juvenile sporophytes, which has easy operation, low cost and high maturation rate of the generated macrogametocytes and can provide a large quantity of the macrogametocytes for the large-scale seedling production of undaria pinnatifida. The method comprises the following steps of: cutting undaria pinnatifida macrogametocytes stored and cultured in a room into pieces and then culturing under the conditions of temperature suitable for the undaria pinnatifida macrogametocytes to grow to be mature, illumination and nutritive salt, wherein when the undaria pinnatifida macrogametocytes grow to be mature, form oocysts in large quantities and ovulate with ovums, a small quantity of the fertilized ovums are germinated to seedlings through parthenogenesis because unfertilized ovums are died by being unfertilized by spermatozoa; and when the somatic cells of the parthenogenesis seedlings are grown to 0.4-0.6 cm, adopting a rhizoid cutting method to enable fronds to lose polarity and constraint on the somatic cells, and inducing the somatic cells of the seedlings to generate the macrogametocytes.
Owner:DALIAN FISHERIES UNIVERSITY

Automated Intracytoplasmic Sperm Injection Assisted Fertilization System

An integrated automated system comprising a microfluidic cassette, and methods of use thereof, for intracytoplasmic sperm injection assisted fertilization. The microfluidic cassette and the integrated automated system provides a complete set-up of human gametes for assisted in vitro fertilization, including proper cell stage recognition, gamete propulsion via microfluidic currents, microinjection of a spermatozoon into an oocyte, and subsequent embryo culture and monitoring, thus allowing widespread distribution of in vitro insemination by favoring affordability.
Owner:CORNELL UNIVERSITY

Method for detecting spermatozoon DNA fragment with spermatozoon chromatin diffusion experiment

The invention discloses a method for detecting sperm DNA fragmentations by a sperm chromatin dispersion test, which comprises the following steps that: 1) a sperm lysis solution is accurately prepared; 2) the sperm lysis solution is placed at a temperature of 4 DEG C and is preserved for 2 weeks, is taken out 1.5 hours earlier before the use, and is placed at a room temperature of 22 DEG C; 3) semen is diluted to 5-10x10<6> / ml; 4) 50 microlitres of diluted semen is taken and placed in a refrigerator for 5 minutes, and the temperature in the refrigerator is 4 DEG C; 5) a microscopic glass is removed after the diluted semen is taken out from the refrigerator, and a glass slide is horizontally immersed into hydrochloric acid for degradation for 7 minutes, and then is taken out; 6) the glass slide taken away in the step 5) is horizontally immersed into the sperm lysis solution in the step 1) for 20 minutes; 7). The glass slide in the step 6) is washed by a buffer liquid for 5 minutes; 8) alcohol is used to respectively dehydrate for 2 minutes; and 9) a Wright method or a Giemsa-Wright method or a sky blue method is used to dye sperms, and the sperm DNA fragmentations are observed and analyzed under an optical microscope. The method realizes the aims of simplicity, quickness, accuracy, low cost, high resolution of sperm DNA detection.
Owner:SHANDONG PROVINCIAL FAMILY PLANNING INST OF SCI & TECH

A micro-fluidic device for selective sorting of highly motile and morphologically normal sperm from unprocessed semen

A microfluidic chip is provided for self-sorting highly motile, morphologically normal sperm cell with high DNA integrity from a fresh semen sample. The sperm self-sorting microfluidic chip has one ormore inlet chambers, and sperm collection outlet chamber(s), and the middle of the channel features various micro-fabricated structures in different geometrical shapes and orientations, with varyingperiodicities and patterns, such as an array of micro-fabricated pillars that facilitate the transport of the active and healthy sperm into the outlet chamber.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV +1

Formative agent of protein complex

The present invention proposes formative agent of protein complex, in which a polyphenol is useful component, and the agent is useful as gene complex, cell adhesion inhibitor or immune tolerogen. The polyphenol of forming the agent is selected from catechin group consisting of epigallocatechin-gallate, tannic acids, or proanto-dianisidine, a protein of the protein complex is selected from proteins consisting of animal proteins composed of polypeptide chain of peptide-combined amino acids, vegetative proteins, nucleus proteins, glycogen proteins, lipo-proteins and metal proteins, the gene complex comprises by compositing genes by polyphenol catechins in order to introduce genes to cells of animals or human bodies, a cell composed of the cell adhesion inhibitor is selected from cells consisting of an animal cell including a stem cell, skin cell, mucosa cell, hepatocyte, islet cell, neural cell, cartilage cell, endothelial cell, epidermal cell, osteocyte or muscle cell isolated from human or animal organism, or sperm, ovum or fertilized egg of domestic animals or fishes and a tissue or an organ for transplantation of the immune tolerogen is selected from the tissue or the organ consisting of skin, blood vessel, cornea, kidney, heart, liver, umbilical cord, bowels, nerve, lung, placenta or pancreas.
Owner:BERTELSMANN MUSIC GROUP

Semi-quantitative sperm concentration rapid self-test kit, prepration method and using method thereof

The invention discloses a semi-quantitative sperm concentration rapid self-test kit, a prepration method and a using method thereof, the semi-quantitative sperm concentration rapid self-test kit comprises a sperm collection box, cell staining solution, physiological saline and a test board, wherein, the sperm collection box consists of a plastic collection box and a sperm liquefying agent, the test board comprises a housing and a substrate, a test hole and a control hole are formed in the center of the housing, water-absorbing paper, a filter membrane and a standard color unit are arranged onthe substrate, and the color of the standard color unit is the color of the test results of adding sperm samples with the concentration of 5 million, 10 million, 20 million, 30 million, 40 million, 60million, 80 million and 100 million / ml into the cell staining solution. The semi-quantitative sperm concentration rapid self-test kit has the advantages that the semi-quantitative sperm concentrationrapid self-test kit can rapidly and accurately detect the concentration of sperm and further diagnose whether a man can produce offspring or not, compared with the traditional diagnosis method, the semi-quantitative sperm concentration rapid self-test kit can save a lot of time, the use is simple, the semi-quantitative sperm concentration rapid self-test kit can be purchased at a pharmacy and used at home, thereby avoiding the feeling of shyness of a user.
Owner:北京望升伟业科技发展有限公司

Decoction for promoting fertility

The invention provides a decoction for promoting fertility. The decoction has functions of nourishing liver and kidney, warming yang and securing essence and can be mainly used for treating male infertility with an excellent effect; the male infertility comprises the following symptoms: sexual disorders such as impotence, spermatorrhea and anejaculation, dysspermia such as oligospermia, low active ratio, dead spermatozoon and nonliquefaction of semen, yang deficiency and the like. Traditional Chinese medicine composition of the decoction is prepared from the following 36 compatible raw materials according to weight: prepared rhizome rehmannia, Chinese yam, dogwood, poria cocos, rhizoma alismatis, salivia chinensis, semen allii tuberose, Chinese wolfberry, rubus idaeus, flatstem milkvetch seed, semen cuscutae, radix achyranthis bidentatae, cistanche, human placenta, bombyx mori, isinglass and the like. The decoction is excellent in effect for treating infertility caused due to poor sperm motility or few sperms, excellent in effect for treating male infertility and quick in curative effect; the decoction is prepared from natural traditional Chinese medicine components, so that the decoction is free of side effect; the formula of the decoction has the functions of strengthening sperms and effectively increasing the number of the sperms.
Owner:苟晓龙

Specimen culturing assembly suitable for use in in-vitro fertilization and in other cell culturing procedures

An assembly is disclosed that is to be used for the culturing of specimens such as embryos and gametes for use in in vitro fertilization. The assembly includes an annular ring which is affixed to the stage of an optical viewing instrument, such as a microscope. The viewing instrument is focused on a point which lies inside of the ring at a predetermined distance from the center of the ring. Circular specimen dishes having a plurality of specimen wells in which the specimens in question are cultured or grown is removably positioned inside of the ring. The specimen wells have bottom walls which are configured so as to ensure that the specimens in the wells will gravitate to the same predetermined position in each of the wells. That position coincides with the focus point of the viewing instrument. When the specimen culturing dishes are placed inside of the ring, they can be rotated inside of the ring to bring the specimens in each well sequentially into the focus point of the viewing instrument whereby the specimens in each dish can be quickly and accurately monitored for growth and development. Other devices are disclosed which enable the rotation of the dish and proper alignment of the specimens in each well with the focus point of the viewing instrument. The assembly can be used for a wide range of specimen culturing. Typical specimens include animal and human cells, tissues, stem cells, embryos, oocytes, immature oocytes, sperm precursor cells, embryonic cells, blastocysts, and spermatozoa.
Owner:COOPERSURGICAL INC +1

Chinese sturgen artificial insemination method and used diluent

The artificial insemination method for Chinese sturgeon adopts diluent A and diluent B as insemination medium and adopts double wet method to make operation. For the first time it uses the diluent A to wash ova and remove ovarian liquid, and for the second time it uses the diluent B to activate ova and spermatozoa and make the spermatozoa be uniformly dispersed to implement insemination. The described diluent includes diluent A and diluent B. The diluent A is made up by using NaCl, Kcl, CaCl2 2H2O, MgCl 6H2O, NaHCo3, Na2Co3, NaOH, citricacid, penicillin, streptomycin and distilled water, and the diluent B is obtained by using distilled water whose volume is 25 times that of diluent A to dilute the diluent A.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Semen analysis

A method for measuring the total sperm concentration (TSC) in a sample is described and includes: (i) placing the sample in a transparent container between a synchronically pulsed light source and a photodetector; and (ii) measuring the optical absorbance of the sample in the range of 800-1000 nm, the TSC of the sample being proportional to the absorbance. Also described is a sampling device for use in optically analyzing a biological fluid, a method for measuring motile sperm concentration (MSC) in a semen sample, a method of determining the average velocity (AV) of sperm cells and a system for analyzing semen quality including means for measuring TSC, means for measuring MSC; and a video visualization system.
Owner:M E S MEDICAL ELECTRONICS SYST
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