82 results about "Spermatozoon" patented technology

The invention provides a sperm automatic detection and tracking method, sperm automatic detection and tracking equipment and a storage medium. The method comprises the following steps: firstly, preprocessing a sperm image, performing rotation bounding box labeling on a head and a tail, and generating a training set containing a center point coordinate, a width, a height and an angle; training a target detection model by using the training set to obtain a model capable of automatically identifying sperms and outputting a rotating bounding box; detecting video frames by using the model, and extracting the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and track continuity, adjacent frame targets are matched, a comprehensive score is generated, the targets are associated, and a continuous track and a unique identifier are formed and used for calculating motion parameters; and judging whether the track is static or low active according to the speed and displacement of the latest N frames, sealing the track to eliminate subsequent matching, and only reserving a non-sealed track to calculate motion parameters. According to the method, interference is effectively avoided, and the tracking accuracy and the reliability of sperm motility parameter calculation are remarkably improved.

The application provides a sperm automatic detection and tracking method, device and storage medium. The method first pre-processes a sperm image, performs head-tail rotation boundary box labeling, and generates a training set containing center point coordinates, width, height and angle; a target detection model is trained using the training set to obtain a model capable of automatically identifying sperm and outputting a rotation boundary box; the model is applied to detect video frames to extract the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and trajectory continuity to match adjacent frames of targets, generate a comprehensive score and associate the targets to form a continuous trajectory and a unique identifier for calculating motion parameters; and then whether the trajectory is stationary or low active is judged according to the speed and displacement of the last N frames, the trajectory is sealed to exclude subsequent matching, and only the non-sealed trajectory is retained to calculate the motion parameters. The method effectively avoids interference, significantly improves the tracking accuracy and the reliability of sperm motility parameter calculation.

An automated method for assessing the ZP binding capacity of sperm from morphological characteristics of sperm in assisted reproduction using deep learning is disclosed. The invention also provides a method for predicting fertilization success based on ZP binding capacity of sperm in clinical assisted reproduction.

This invention belongs to the field of membrane separation and functional membrane materials technology, and discloses a chiral graphene oxide-based separation membrane, its preparation method, and its application. The membrane is composed of graphene oxide and / or reduced graphene oxide sheets and a single-stranded DNA-single-walled carbon nanotube complex. The single-stranded DNA helically winds around the outer surface of the single-walled carbon nanotubes to form a one-dimensional chiral nanochannel and intercalates between the sheets, forming a three-dimensional chiral channel network connected in the membrane thickness direction. The preparation method includes: heating and decohexing salmon sperm double-stranded DNA in a urea-containing buffer to obtain single-stranded DNA; dispersing single-walled carbon nanotubes and then complexing them with the single-stranded DNA; further mixing with a graphene oxide or reduced graphene oxide dispersion; and vacuum filtration to form the membrane. This membrane exhibits a high separation factor for chiral enantiomers such as L / D-phenylalanine, with an excess value of up to 99.95% for L-phenylalanine enantiomers after multi-stage separation, making it suitable for the efficient separation of chiral drugs and amino acids.

1. Name of the product in this design: Sperm Quality Analyzer. 2. Purpose of this design: The product is intended for use by research institutions and medical institutions for sperm concentration detection, motility analysis, sperm morphology identification, and determination of semen pH and DNA fragmentation rate in semen samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D rendering 2.

The invention discloses a yak frozen semen freezing process intelligent control method based on dynamic cooling curve optimization, and relates to the field of animal frozen semen preservation, and the yak frozen semen freezing process intelligent control method comprises the following steps: arranging a plurality of frozen semen tubes filled with consumed cattle semen on an annular tray with a cold source in the center in a radioactive manner, and uniformly arranging the plurality of frozen semen tubes in a surrounding manner in each radial distance interval; in the freezing process, cold energy is output according to a preset cooling program, and the annular tray is driven to rotate; unfreezing operation is executed, sperm motility values are measured, interval motility values of the sperm motility values of the frozen sperm tubules in all the radial distance intervals are counted, a temperature curve at the radial distance interval with the highest interval motility value is determined, a cooling program is modified in multiple rounds of experiments, and the process is repeated, so that the temperature curve is determined. And clustering the obtained temperature curves and selecting an optimal target temperature curve to perform freezing control. According to the method, the problem that the optimal dynamic cooling curve is difficult to efficiently obtain in the freezing process of the frozen yak sperms is solved.

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

The present invention relates to the identification and / or selection of sperm, and in particular a means for identifying and / or selecting the fittest sperm for use in assisted reproductive technologies.

The invention provides an SNP (Single Nucleotide Polymorphism) marker for influencing the rapid forward movement sperm proportion of a pig. The sequence of nucleic acid of the SNP marker is shown as SEQ ID No.1, and the site of the SNP marker is located at the 301st site from the 5'end on the nucleic acid shown as SEQ ID No.1, corresponds to the 3803902 site from the 5 'end on the NW018084979.1 chromosome of the international pig genome of the version 11.1, and is C or T.

The present invention relates to a method for analysing a spermatozoon, comprising a step of extracting the nucleic acids from the spermatozoa contained in a first sample of spermatozoa previously obtained from a human subject; a step of measuring the level of expression of at least one marker gene chosen from the group consisting of AURKA, CFAP46, CCDC60, CCDC88B, HDAC4, CACNA1C, CACNA1H, CARHSP1, DNAH2 and SPATA18 from the extracted nucleic acids; and a step of determining the existence of an expression differential of the at least one marker gene in relation to a control. It also relates to a method for selecting spermatozoa and a method for evaluating the quality of a sperm.

The invention relates to the technical field of lion-head goose breeding, in particular to a method for screening and identifying genes related to lion-head goose sperm motility regulation and control, which comprises the following steps: dividing healthy lion-head male geese in a breeding period into a high sperm motility group and a low sperm motility group according to sperm motility, collecting two groups of testicular tissues and sperm samples, and preserving the testicular tissues at low temperature for molecular detection; performing transcriptome sequencing on the testicular tissue to obtain mRNA and non-coding RNA expression data; screening two groups of nucleic acid molecules with significant differential expression, and determining candidate genes through functional enrichment analysis of reproductive development related biological pathways; and verifying the authenticity of the expression difference of the candidate gene through fluorescent quantitative PCR, and finally determining the target gene. According to the method, related data are obtained through sequencing, the candidate genes are determined by screening differential molecules through enrichment analysis and PCR verification, accurate identification of a related gene system is achieved, and the problems of missing detection and insufficient accuracy caused by lack of transcriptome sequencing in a traditional method are solved.

The purpose of the present invention is to provide spermatozoa having high motility. The present invention is a composition for treating sperm that comprises a mesenchymal stem cell culture supernatant. The composition for spermatozoa treatment of the present invention is used as a sperm preparing solution, a sperm diluting solution, a sperm storage solution, an artificial insemination solution, an in vitro fertilization solution, a solution for improving sperm motility, or a solution for retaining sperm fertilizing capabilities. Furthermore, mesenchymal stem cells in the mesenchymal stem cell culture supernatant comprised in the composition for treating sperm are preferably derived from adipose tissue, derived from umbilical cord tissue, or derived from bone marrow tissue.

The invention discloses a method for establishing an oligoasthenospermia model by using alpha-ketoglutaric acid. Alpha-ketoglutaric acid is added into drinking water of animals to obtain the oligoasthenospermia model. According to the invention, alpha-ketoglutarate (alpha-KG) is added into drinking water of a male mouse, so that the oligoasthenospermia mouse model is successfully established. The invention provides a new thought and clue for deeply researching the pathogenesis of oligoasthenospermia and researchers of male reproductive health, and has important scientific and medical values for understanding the mechanism of male infertility, developing a new therapy, evaluating the treatment effect and promoting related clinical research.

The present application relates to sperm movement technical field, especially a kind of method and device for measuring sperm head rotation frequency, comprising: S1, the image information of sample to be measured is obtained, based on the image information, select target sperm;S2, based on the target sperm, the light spot of the target sperm head after the target sperm is captured to head in the preset optical tweezers light beam is obtained;S3, based on the light spot of the target sperm head, the light spot intensity variation information is obtained, the light spot intensity variation information is converted into electrical signal data, the electrical signal data is carried out power spectrum analysis, and the rotation frequency of the target sperm head is obtained.The present application carries out real-time monitoring and analysis to sperm movement characteristics, can quickly obtain sperm movement characteristics, and the accuracy of measurement result is high.

The application provides a general single-stage sperm morphology image segmentation method and system, which comprises the following steps: obtaining a stained or unstained sperm microscopic image; inputting the sperm microscopic image into a single-stage segmentation network model, outputting a domain robust shared feature map through multi-view transformation and adaptive fusion; after multi-scale feature extraction and aggregation, a prediction head is used to output a semantic segmentation and / or instance-aware component segmentation result in parallel; tail skeleton features are extracted and topological optimization is performed based on a continuity constraint loss to maintain tail connectivity. The application enhances the cross-domain generalization capability by structure-appearance decoupling, and uses topological-aware learning to constrain the tail continuity, thereby solving the problems of weak generalization between stained and unstained images and tail segmentation fracture in high-density overlapping scenes in the prior art, and improving the clinical applicability and segmentation integrity.

The present application relates to the field of animal breeding technology, in particular to the application of ursolic acid in the frozen semen preservation of sheep. The present application applies ursolic acid in the frozen semen preservation of sheep. The addition of 0.5-2.0 micromole / L ursolic acid in the frozen diluent can improve the quality of frozen semen preservation of sheep and goats, and can improve the sperm motility and vitality after thawing, which helps the thawed sperm to pass through the vagina, uterus and enter the oviduct to complete fertilization, improves the insemination success rate of frozen sperm, and provides technical support for strengthening the protection of local sheep breed resources.

The present application relates to the technical field of image processing, and discloses a light staining sperm microscopic image light-weight style consistency preprocessing method and system, comprising: acquiring a light staining sperm microscopic image, performing color normalization on the light staining sperm microscopic image according to a deep staining sperm microscopic image, performing local contrast enhancement on the normalized light staining sperm microscopic image according to local contrast of a sperm head, a tail and a boundary region, performing brightness correction on the enhanced light staining sperm microscopic image according to the definition of the sperm head, the middle section and the tail structure and the background stability, and obtaining a light staining sperm microscopic image after light-weight style consistency preprocessing. The present application can solve the problems of staining difference, uneven brightness and boundary blur through image preprocessing without increasing the number of samples and additional training burden, improve the overall quality of the light staining sperm microscopic image, and improve the detection performance of the subsequent model.

The invention discloses a special biscuit for improving sperm fertilization ability of boars under heat stress and a processing method thereof, and the biscuit comprises the following components by weight: 10% of grease; the grease comprises DHA (docosahexaenoic acid) and EPA (eicosapentaenoic acid), and the weight ratios of the DHA and the EPA in the grease are respectively 30% and 35%; 8%-18% of an amino acid component; the weight ratio of lysine to methionine to tryptophan to threonine to arginine in the amino acid component is 100 to (26 to 38) to (18 to 32) to (70 to 90) to (60 to 120); the vitamin component comprises 0.6%-1.0% of vitamin A, 1%-3% of L-carnitine, 0.5%-1% of vitamin E and 0.5%-1% of vitamin D; the trace element components comprise 0.01%-0.1% of nano selenium, 0.1%-1% of nano zinc and 0.02% of organic chromium; and the balance of corn starch. The special biscuit can improve the sperm motility of the boars and improve the reproductive nutrition status of the boars, the special biscuit is designed into independent quantitative solid blocks, and the problems that powdery additives are inaccurate in metering, uneven in mixing and waste in throwing can be solved.

The present invention relates to sperm image processing technology. Disclosed are a human sperm image color block impurity removal method, and an apparatus. The method comprises: acquiring a sperm image processed with a staining reagent; using a pre-trained instance segmentation model to perform segmentation processing on sperm heads, acrosome regions, and acrosome posterior regions in the sperm image, and performing post-processing, matching and assembling on segmentation results to obtain segmentation and identification information of all individual independent sperms in the image; and searching for color block impurities in segmentation and identification results, and deleting same, so as to obtain sperm segmentation results having no color block impurities. The method of the present invention has the advantages of simplicity and good operation effect, and is very suitable for practical use in industrial scenarios. In addition, by means of the method for filtering color block impurities, color block impurities of sperms segmented and recognized in a sperm image can be accurately and effectively deleted, and final sperm image segmentation and recognition results have no color block impurities, thereby improving the accuracy of computer-assisted sperm morphology analysis.

The application discloses application of ACTRT1 as a target to detection and treatment of headless sperm. In the application, ACTRT1 gene mutation causes deletion of ACTRT1 functional protein, may affect connection of sperm head and tail, and finally induces headless sperm.

The application discloses an insect sperm detection and tracking method and system based on fine-grained subspace perception, relates to the technical field of insect reproduction prevention and control, and performs boundary box labeling on an insect sperm image to establish a data set; a preset sperm target detection model is trained through the data set; a feature map output by a fine-grained subspace perception attention module in the model is combined with the insect sperm image labeled through the boundary box to obtain a tracking vector; the target boundary box output by a detection head in the model and the tracking vector are taken as inputs, the inputs are input into a preset sperm target tracking model, Mahalanobis distance and appearance distance are calculated based on a predicted state and an observation vector, a comprehensive matching cost is constructed through the Mahalanobis distance and the appearance distance, a global optimal matching scheme is calculated based on the comprehensive matching cost and a Hungarian algorithm, the trajectory state of a successfully matched target is updated, and a tracking result is output. The application realizes detection and tracking of insect sperm.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a sheep semen cryopreservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in the cryopreservation of the sheep semen for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the semen quality of the frozen and unfrozen sheep semen and improve the fertilization ability of the frozen semen; such as sperm motility, sperm movement performance, sperm viability, sperm plasma membrane integrity rate, sperm acrosome integrity rate, sperm antioxidant capacity, sperm ATP level, sperm early apoptosis incidence rate, sperm in-vitro fertilized ovulation rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm cryopreservation diluent.

A method of determining fusogenic potential of sperm is provided. The method comprising: (a) contacting a biological sample comprising sperm with somatic cells expressing ectopic JUNO under conditions which allow binding of the sperm to the JUNO; and subsequently (b) detecting multinucleation in the somatic cells, wherein a level of the multinucleation is indicative of the fusogenic potential of the sperm.

The invention discloses a low-density sperm braking method without a PVP medium in ICSI fertilization, and relates to the field of assisted reproduction therapy, and the method comprises the following steps: step 1, diluting a sperm suspension until the density is 0.1 * 10 < 6 > / ml, and placing the diluted sperm suspension in an in-vitro operating solution without PVP; step 2, under an inverted microscope, directly pressing and braking the tail of the sperm in the in-vitro operating fluid by using a microinjection needle, so that the tail of the sperm is folded or curled to deform; and step 3, transferring the braked sperms into operation liquid drops containing ovum to complete microinjection, and performing tail pressing operation in an isotonic operation liquid without PVP (Polyvinyl Pyrrolidone), so that the tail parts of the sperms present visible curled deformation or tail folding. The sperm motility destruction rate is 100% (residual motility is completely prevented from interfering fertilization), and the tail cell membrane rupture rate is 100% (it is ensured that activation factors in sperms are fully released).

The invention relates to the technical field of health-care food, in particular to a sea cucumber peptide solid beverage capable of enhancing immunity and a production process of the sea cucumber peptide solid beverage. The sea cucumber peptide health-care food is prepared from the following raw materials: more than or equal to 50% of sea cucumber peptide, more than or equal to 10.55% of rhizoma polygonati powder, more than or equal to 5% of fructus lycii powder, more than or equal to 5% of mulberry powder, more than or equal to 4% of herba cistanche powder, more than or equal to 1% of eucommia male pollen, more than or equal to 1% of gynura procumbens powder, more than or equal to 1% of ginseng powder, more than or equal to 0.5% of maca powder and Through scientific compatibility of the sea cucumber peptide, the rhizoma polygonati, the herba cistanche and other components, the content of lactic acid and malondialdehyde can be reduced, the testosterone level of serum and the sperm quality can be remarkably improved, deep synergy of fatigue resistance, oxidation resistance and improvement of male physiological indexes is achieved, the small molecule characteristic that the high-content sea cucumber peptide is easily absorbed by intestinal tracts is utilized, and the health care effect is achieved. And in cooperation with protein and rich B vitamins and minerals, the quick conversion requirement of organisms on high-concentration nutrients is effectively met.

The application belongs to the field of biological medicine, and particularly relates to a kind of Bungarus multicinctus venom protein and a preparation method and application thereof.The application provides a new sperm motility promoter, which is derived from Bungarus multicinctus venom and has the application potential to be developed into a new sperm motility promoter.

The invention discloses a rapid breeding method for generating larimichthys crocea sperms based on spermatogonial stem cell transplantation. The rapid breeding method comprises the following steps: step S1, performing whole cryopreservation on larimichthys crocea testis; s2, separating the spermatogonial stem cells of the pseudosciaena crocea; s3, dyeing the spermatogonial stem cells of the pseudosciaena crocea; step S4, treatment of nibea albiflora receptors; and S5, transplanting the dyed donor larimichthys crocea spermatogonial stem cells into the nibea albiflora receptor testis. According to the method, when the nibea albiflora acceptor is treated, the traditional busulfan concentration of 40 mg / kg, twice injection and 35 DEG C high-temperature assistance is optimized into 60 mg / kg, single injection and 22 DEG C normal-temperature culture, optimal parameters induced by busulfan are screened out through experiments, the operation process is simplified under the action of ensuring that endogenous germ cells of the acceptor are reduced and immunological rejection is reduced, and the method is suitable for large-scale popularization and application. The harsh requirement of a high-temperature environment is avoided, and meanwhile the field planting efficiency of donor cells in receptors and the survival rate of the receptors are improved.

This invention belongs to the field of animal husbandry technology and relates to a method for improving the quality of frozen semen from Lanzhou fat-tailed sheep based on ergothioneine. This application, through optimized combination of diluent, 8 layers of gauze wrapping for cooling, 4 cm fumigation height, and 8 mM ergothioneine, achieved a sperm motility rate of 45.80±3.62%, an acrosome integrity rate of 52.32±2.28%, and a plasma membrane integrity rate of 55.38±3.55% after thawing. All indicators are significantly better than those of the traditional single-optimization group.