274 results about "Spermatozoon" patented technology

An optical pattern-driven light induced dielectrophoresis (DEP) apparatus and separation methods are described which provide for the manipulation of particles or cells and selection based on traits correlated with the DEP response. Embodiments of the apparatus use DEP electric field patterns in combination with microfluidic laminar flows to measure response, separate, segregate and extract particles from heterogeneous mixtures according to the relative response of the particles to one or more DEP fields without damaging living cells. The preferred OET-DEP devices generally comprise a planar liquid-filled structure having one or more portions which are photoconductive to convert incoming light to a localized virtual electrode with a DEP electric field gradient of selected intensity along with input and a plurality of output fluidic channels. The light patterns are dynamically generated to provide a number of manipulation structures that can manipulate single particles and cells or groups of particles / cells. The methods are particularly suited for selecting and extracting the best sperm and embryo candidates based on fitness for use with existing artificial reproduction procedures and excluding defective or non-viable gametes.

The invention provides a decoction for promoting fertility. The decoction has functions of nourishing liver and kidney, warming yang and securing essence and can be mainly used for treating male infertility with an excellent effect; the male infertility comprises the following symptoms: sexual disorders such as impotence, spermatorrhea and anejaculation, dysspermia such as oligospermia, low active ratio, dead spermatozoon and nonliquefaction of semen, yang deficiency and the like. Traditional Chinese medicine composition of the decoction is prepared from the following 36 compatible raw materials according to weight: prepared rhizome rehmannia, Chinese yam, dogwood, poria cocos, rhizoma alismatis, salivia chinensis, semen allii tuberose, Chinese wolfberry, rubus idaeus, flatstem milkvetch seed, semen cuscutae, radix achyranthis bidentatae, cistanche, human placenta, bombyx mori, isinglass and the like. The decoction is excellent in effect for treating infertility caused due to poor sperm motility or few sperms, excellent in effect for treating male infertility and quick in curative effect; the decoction is prepared from natural traditional Chinese medicine components, so that the decoction is free of side effect; the formula of the decoction has the functions of strengthening sperms and effectively increasing the number of the sperms.

An assembly is disclosed that is to be used for the culturing of specimens such as embryos and gametes for use in in vitro fertilization. The assembly includes an annular ring which is affixed to the stage of an optical viewing instrument, such as a microscope. The viewing instrument is focused on a point which lies inside of the ring at a predetermined distance from the center of the ring. Circular specimen dishes having a plurality of specimen wells in which the specimens in question are cultured or grown is removably positioned inside of the ring. The specimen wells have bottom walls which are configured so as to ensure that the specimens in the wells will gravitate to the same predetermined position in each of the wells. That position coincides with the focus point of the viewing instrument. When the specimen culturing dishes are placed inside of the ring, they can be rotated inside of the ring to bring the specimens in each well sequentially into the focus point of the viewing instrument whereby the specimens in each dish can be quickly and accurately monitored for growth and development. Other devices are disclosed which enable the rotation of the dish and proper alignment of the specimens in each well with the focus point of the viewing instrument. The assembly can be used for a wide range of specimen culturing. Typical specimens include animal and human cells, tissues, stem cells, embryos, oocytes, immature oocytes, sperm precursor cells, embryonic cells, blastocysts, and spermatozoa.

A method for measuring the total sperm concentration (TSC) in a sample is described and includes: (i) placing the sample in a transparent container between a synchronically pulsed light source and a photodetector; and (ii) measuring the optical absorbance of the sample in the range of 800-1000 nm, the TSC of the sample being proportional to the absorbance. Also described is a sampling device for use in optically analyzing a biological fluid, a method for measuring motile sperm concentration (MSC) in a semen sample, a method of determining the average velocity (AV) of sperm cells and a system for analyzing semen quality including means for measuring TSC, means for measuring MSC; and a video visualization system.