266 results about "Spermatozoon" patented technology

Isolated non-naturally occurring populations of spermatozoa (15) having high purity and technologies to differentiate spermatozoa (28) based on characteristics such as mass, volume, orientation, or emitted light including methods of analysis and apparatus such as beam shaping optics (30) and detectors (32).

Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformity stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.

The invention discloses a freezing method of pig sperms. Unexpectedly, the DNA (deoxyribonucleic acid) completeness and the in vitro fertilization (IVF) rate of unfrozen pig sperms can be obviously improved by using 9(w/v)% of low-density albumin for treatment during the freezing process.

The present invention relates to a method and a system for producing a mammalian pre-embryo and a stem cell having a better quality than prior art methods. The system comprises means for obtaining a mammalian oocyte, and means for obtaining a mammalian spermatozoa, and an apparatus having at least two separate air-tight chambers, for which the oxygen tension of one chamber may be changed independent of the oxygen tension of the other chamber, said at least two separate air-tight chambers constitute a main chamber and at least one residence chamber. The method for in vitro producing a mammalian pre-embryo comprising the steps: a1) providing a mammalian oocyte, a2) providing a mammalian spermatozoa, b) culturing the oocyte and the spermatozoa, c) fertilizing the oocyte with the spermatozoa obtaining a fertilized oocyte, and d) allowing cell-division of the fertilized oocyte obtaining a multicellular pre-embryo wherein at least one of the steps a1) or a2) is conducted at an oxygen tension below 15%, or e) allowing cell-division of the fertilized oocyte obtaining a multicellular pre-embryo, wherein the culture is performed at an oxygen tension allowing cultivation of the cells and wherein at least one of the steps comprises a change in the oxygen tension. Stem cells are produced from the multicellular pre-embryo.

The invention discloses a method for inducing cleavage gynogenesis of fish fry of cynoglossus semilaevis, which comprises the steps of cynoglossus semilaevis sperm freezing storage, ultraviolet radiation of sperms, and induction and identification of the cleavage gynogenesis of the fish fry. The cynoglossus semilaevis sperm freezing storage method is established, and a method for inhibiting the first cleavage to induce the multiplication of embryo chromosome of the gynogenesis of the cynoglossus semilaevis through hydrostatic pressure is adopted so as to screen effective dilution and freezing method for the cynoglossus semilaevis sperm freezing storage and screen the hydrostatic pressure shock starting time, hydrostatic pressure and processing time suitable for the cleavage gynogenesis of cynoglossus semilaevis; and an identification method for the gynogenesis of the fish fry of the cynoglossus semilaevis is established. In the method, the fish fry of the cynoglossus semilaevis through cleavage gynogenesis is obtained for the first time, and the inductivity of the gynogenesis of the fish fry reaches 0.25 percent; and the method has the characteristics of advancement, high efficiency, reliability and reliability, can be used for solving the problems that male cynoglossus semilaevis grows slowly, the ratio of female cynoglossus semilaevis is low and the culture cost is high, and has significant application value and wide promotion and application prospect in the aspects of breeding of cynoglossus semilaevis fry, sex control, culture of all female fry, pure line breeding and the like.

A hybridization method between Chinese spiny sea cucumber and Russian (or korean) one for preparing the hybrid one with high growth speed, stress resistance, survival rate and nutritive value includes such steps as using special culture technique to make them mature symultaneously, artificial oxytocia to obtain their spermatozoa and ova, artificial fertilization, and culturing fertilizer ova until they become laval plankton.

The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A/G and G/A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.

The invention relates to a sperm acrosome zone Raman spectrum peak and a use thereof. The Raman spectrum peak is represented by an abstract figure shown in the specification, and can be used for the fertilization function assessment and detection and the theory researches of a sperm. The invention also relates to a sperm fertilization function assessment method. The method comprises the following steps: 1, preparing a spermatic slice; 2, scanning the single sperm acrosome zone on the spermatic slice at a laser excitation wave of 532nm under a laser power of 5mW three times to obtain a Raman data spectrum peak; and 3, contrasting the Raman data spectrum peak of a detection sample with the Raman data spectrum peak of the above sperm acrosome zone to determine that whether the sperm has a fertilization function or not. The method provides a standard Raman spectrum peak for determining the sperm fertilization potential, and the determination result of the method is accurate and reliable, and has a high credibility, so the method is a new sperm fertilization potential detection method; and a separate sperm can be controlled by the method, so there is no need to specially process the sperm, the damage of the sperm can controlled in an extremely low range, and the sperm can be directly used for subsequent researches or auxiliary reproduction treatment.

The invention relates to a culture solution for bovine IVF and a method for improving bovine IVF. The method for bovine IVF comprises the following steps: washing a mature cumulus-oocyte complex in afertilization culture solution, transferring the mature cumulus-oocyte complex into the fertilization culture solution, and putting the fertilization culture solution into an incubator for later use;taking a frozen seminal tubule from liquid nitrogen, performing unfreezing in a water bath at 37 DEG C, injecting seminal fluid into a centrifuge tube containing a seminal fluid preparation culture solution, and discarding a supernatant after centrifugation; adding the seminal fluid preparation culture solution into the centrifuge tube, resuspending sperm precipitate, and taking a proper sperm suspension for sperm counting; and adding the sperm suspension with the calculated volume into fertilization culture liquid drops containing oocytes, placing a culture tray into the incubator, and carrying out sperm-ovum co-incubation to complete IVF operation for embryo in-vitro culture. The fertilization culture solution and the seminal fluid preparation culture solution contain sodium pyruvate, heparin and other components. The method can significantly improve the efficiency of bovine IVF.

The invention provides a two-layer percoll density gradient centrifugal separation method of boar sperms. The method is suitable for normal sperm specimen and sperm specimen with low sperm density or low sperm motility, and is a simple, convenient and fast sperm treatment method. The method can be used for remove leucocytes, bacteria, fragments and anti-sperm antibodies in the sperms and improving the delayed sperm liquefaction, the sperm in-vitro capacitation and the like, so that the sperms can effectively fertilize ova to improve the pregnancy rate, and the percentage of the sperms with motility and normal shape in the obtained sperms is about 95%. The method provided by the invention is suitable for screening sperms with motility from fresh boar sperms and diluted sperms, thus providing high-quality sperms for the study of boar sperm capacitation mechanism and in-vitro fertilization.

The invention discloses in vitro human sperm culture solution for improving sperm motility and application thereof. The culture solution contains fructose 6-phosphoric acid (F6P). The in vitro human sperm culture solution for improving the sperm motility of human sperms is used for culturing the purified human sperms which leave from a human body; the vitality of normal human sperms is capable of maintaining sperm movement and can be obviously improved; and a meaningful reference is provided for improving and assisting the reproductive technology. The F6P is directly used for cultivating and diluting the culture solution, and does not relate to organic solvents such as ethanol or dimethylsulfoxide (DMSO) and the like; the toxic action of the ethanol or the DMSO on the sperms is avoided; by adopting the F6P added to the sperm culture solution, the vitality of the sperms of clinical patients with asthenospermia can be obviously improved; the improvement degree of the vitality is the most obvious for severe oligospermia; the activity of a part of cultured sperms reaches the normal sperm vitality range; and the improvement degree of the vitality for the severe oligospermia is the most obvious. This fully demonstrates that low motility of the sperms of the patients with asthenospermia can be improved by using of the F6P.

The invention discloses a method for detecting sperm DNA fragmentations by a sperm chromatin dispersion test, which comprises the following steps that: 1) a sperm lysis solution is accurately prepared; 2) the sperm lysis solution is placed at a temperature of 4 DEG C and is preserved for 2 weeks, is taken out 1.5 hours earlier before the use, and is placed at a room temperature of 22 DEG C; 3) semen is diluted to 5-10x10<6>/ml; 4) 50 microlitres of diluted semen is taken and placed in a refrigerator for 5 minutes, and the temperature in the refrigerator is 4 DEG C; 5) a microscopic glass is removed after the diluted semen is taken out from the refrigerator, and a glass slide is horizontally immersed into hydrochloric acid for degradation for 7 minutes, and then is taken out; 6) the glass slide taken away in the step 5) is horizontally immersed into the sperm lysis solution in the step 1) for 20 minutes; 7). The glass slide in the step 6) is washed by a buffer liquid for 5 minutes; 8) alcohol is used to respectively dehydrate for 2 minutes; and 9) a Wright method or a Giemsa-Wright method or a sky blue method is used to dye sperms, and the sperm DNA fragmentations are observed and analyzed under an optical microscope. The method realizes the aims of simplicity, quickness, accuracy, low cost, high resolution of sperm DNA detection.

The invention discloses a semi-quantitative sperm concentration rapid self-test kit, a prepration method and a using method thereof, the semi-quantitative sperm concentration rapid self-test kit comprises a sperm collection box, cell staining solution, physiological saline and a test board, wherein, the sperm collection box consists of a plastic collection box and a sperm liquefying agent, the test board comprises a housing and a substrate, a test hole and a control hole are formed in the center of the housing, water-absorbing paper, a filter membrane and a standard color unit are arranged onthe substrate, and the color of the standard color unit is the color of the test results of adding sperm samples with the concentration of 5 million, 10 million, 20 million, 30 million, 40 million, 60million, 80 million and 100 million/ml into the cell staining solution. The semi-quantitative sperm concentration rapid self-test kit has the advantages that the semi-quantitative sperm concentrationrapid self-test kit can rapidly and accurately detect the concentration of sperm and further diagnose whether a man can produce offspring or not, compared with the traditional diagnosis method, the semi-quantitative sperm concentration rapid self-test kit can save a lot of time, the use is simple, the semi-quantitative sperm concentration rapid self-test kit can be purchased at a pharmacy and used at home, thereby avoiding the feeling of shyness of a user.

The invention relates to a cross method between large yellow Crocker and GuangDong large yellow Crocker. Wherein, their crossed generation has high grow speed and the application in long transmission; the invention selects GuangDong large yellow crocker as father, whose belly can be pressed to flow sperm; preparing dilute solution; compressing its belly to absorb sperm, whose activity is higher than 90% to be frozen and stored; mixing the dilute solution and anti-freeze agent, adding sperm, storing in frozen storage tube, sealing and putting in 4Deg. C; arranging it above the liquid nitrogen, reducing temperature and moving it into liquid nitrogen; selecteing the wide GuangDong large yellow crocker as mother; when its ovogen is mature, defreezing the sperm, and taking the freeze storage tube from liquid nitrogen to be put into room-temperature fresh water to be defrozen, activating it with seawater; artificial inseminating and washing zygote with seawater, removing sink zygote, and charging air and cultivating.