Research method of application of wedelolactone to preparation of lung cancer treating products

The invention relates to a technology of wecarolide and product, which is applied in the research field of the application of werethrin in the preparation of products for the treatment of lung cancer, and can solve the problems of unclear inhibitory activity of lung cancer cells and inducing apoptosis mechanism.

Pending Publication Date: 2018-12-14
道赛尔生物科技(武汉)有限公司
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Problems solved by technology

However, the inhibitory activity and apoptosis-inducing mec

Method used

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  • Research method of application of wedelolactone to preparation of lung cancer treating products
  • Research method of application of wedelolactone to preparation of lung cancer treating products
  • Research method of application of wedelolactone to preparation of lung cancer treating products

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Embodiment Construction

[0018] The present invention will be described in detail below in conjunction with specific embodiments.

[0019] This embodiment includes the following steps:

[0020] Material preparation steps, materials include

[0021] Human non-small cell lung cancer A549 cells were purchased from the Cell Bank of the Chinese Academy of Sciences;

[0022] Wedelia lactone was purchased from China Standard Materials Network;

[0023] RPMI-1640, FBS, purchased from Life company;

[0024] p-Caspase3 antibody, p53 antibody, p-STAT1 antibody, STAT1 antibody were purchased from Abcam;

[0025] CCK-8 kit, AnnexinV / PI kit, reverse transcription kit, and RIPA lysate were purchased from Biyuntian.

[0026] The steps of cell culture include resuscitating A549 cells, culture in RPMI-1640 containing 10% FBS, and the conditions are 37°C, 5% CO 2 , subculture once every 2-4 days, preferably 3 days;

[0027] The steps of CCK-8 method detection cell proliferation inhibition rate are as follows:

[...

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Abstract

The invention belongs to the field of biotechnology, and particularly relates to a research method of application of wedelolactone to preparation of lung cancer treating products. The invention researches influence of the wedelelactone on the activity, the cycles and the apoptosis of non-small cell lung cancer A549 cells as well as intervention to p-Caspase3, p53, p-STAT1 and STAT1 gene expressionof the cells, explores the anti-tumor effect of the wedelolactone on the A549 cells and the mechanism thereof, provides the research method of the application of the wedelolactone to the preparationof the lung cancer treating products, and provides a theoretical basis for future research on the non-small cell lung cancer treating mechanism of the traditional Chinese medicine. The research methodcomprise the following steps: performing cell culture, detecting the cell proliferation inhibiting rate by a CCK-8 method, detecting the cycles and the apoptosis of the cells through propidium iodideand Annexin-PI staining and detecting gene transcription and translation of the cells through RT-PCR and Western blot.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a research method for the application of wedelia lactone in the preparation of products for treating lung cancer. Background technique [0002] Non-small cell lung cancer is the most common type of lung cancer, and currently ranks first in the mortality rate of malignant tumors in my country. Therefore, it is imminent to take active and effective measures to prevent and treat non-small cell lung cancer. In recent years, Western medicine is mainly used to treat non-small cell lung cancer clinically, and most western medicines are prone to strong dependence and serious side effects after long-term use. Therefore, the importance of research on the treatment of non-small cell lung cancer with traditional Chinese medicine has become increasingly prominent. [0003] Since wedelia lactone was first isolated and purified from Wedelia calendula in 1956, it has been regarded as a ...

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Application Information

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IPC IPC(8): C12N5/09C12Q1/02
CPCG01N33/5011C12N5/0693C12N2500/30
Inventor 黄祥宏赵成诚张强刚
Owner 道赛尔生物科技(武汉)有限公司
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