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Activity detection method for monilinia fructicola

A brown rot fungus and activity detection technology, applied in the direction of measuring devices, test sample preparation, instruments, etc., can solve the problems of strong subjective dependence and long time consumption of operators, and achieve domestic ecological production safety, simple operation, The effect of improving efficiency

Inactive Publication Date: 2019-07-19
SHENZHEN AUDAQUE DATA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection of the activity of the American-Australian drupe rot fungus mainly relies on the traditional morphological identification method. This method needs to cultivate the samples to be tested. Meet the needs of actual inspection and quarantine work

Method used

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  • Activity detection method for monilinia fructicola
  • Activity detection method for monilinia fructicola
  • Activity detection method for monilinia fructicola

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0058] 1. Screening of fluorescent dyes

[0059] 1. Materials and Equipment

[0060] In this case, the American-Australian stone fruit brown rot strain 12470 and strain 127255 were tested respectively; among them, the strain 12470 was intercepted by the Shenzhen Entry-Exit Inspection and Quarantine Bureau in Australian nectarines on March 6, 2017; the strain 127255 was purchased from CBS, The source of the strain is Australia.

[0061] In this case, a total of 10 fluorescent dyes were used to detect the activity of the spores of the American-Australian drupe rot fungus. The details of the fluorescent dyes are shown in Table 1.

[0062] Table 1 Fluorescent dyes to be tested

[0063]

[0064]

[0065] The main reagents used in this example include: dimethyl sulfoxide (abbreviated as DMSO) was purchased from Shanghai Sangon Bioengineering, methanol (Methanol) was purchased from Xilong Chemical Co., Ltd., acetone (Acetone) was purchased from BURDICK&JACKSON, absolute ethan...

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Abstract

The invention discloses an activity detection method for monilinia fructicola. The activity detection method comprises the step of adopting a fluorescent dye to dye a monilinia fructicola spore, and judging the activity according to the dying conditions, wherein the fluorescent dye is propidium iodide and / or fluorescein diacetate, the dying condition of the propidium iodide is carrying out 4min lucifugal dying on the spore at a final concentration of 0.00025 mg / mL, and the dying condition of the fluorescein diacetate is carrying out 16min lucifugal dying on the spore at a final concentration of 0.0025 mg / mL. The activity detection method is capable of directly observing the life or death of the spores so that the operation is simple; and the whole detection process is within 30 min, so that the activity detection efficiency is improved. The activity detection method has the advantages of being rapid, correct, sensitive and good in repeatability, is capable of replacing the traditionalmorphological detection, and provides a novel rapid and effective detection method and way for the departments of port quarantine, agricultural production, plant protection and the like.

Description

technical field [0001] The application relates to the field of detection of the activity of the American-Australian type stone fruit brown rot fungus, in particular to a method for detecting the activity of the American-Australian type stone fruit brown rot fungus. Background technique [0002] Monilinia fructicola (Winter) Honey is a kind of malignant fungus that harms many kinds of fruits. Its main host is Rosaceae fruit trees. Pears, pears, nectarines, and plums are the most harmful. In the field, the main overwintering method of the pathogen is by the infected fruits and branches. The warm and humid weather in spring is suitable for the production of conidia and the germination of conidia. The pathogen will infect and usually invade from the wound. It can also be directly infected on the peel, and the initial symptoms are mild. There are only small brown lesions on the fruit, but under suitable conditions, the whole fruit can be rotted within a few days, and a large nu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/30
CPCG01N1/30G01N21/6428G01N2021/6439
Inventor 高瑞芳朱子钦李清王颖章桂明
Owner SHENZHEN AUDAQUE DATA TECH
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