72 results about "Chlamys farreri" patented technology

The invention provides a substratum used in an artificial breeding and production process of crassostrea gigas and a manufacturing method of the substratum, and provides a method for cultivating the crassostrea gigas by using the substratum. The manufacturing method of the substratum comprises the following steps: cutting and bundling polypropylene packing straps; then wrapping the outer surface of the polypropylene packing straps with mixed cement paste; and air-drying the polypropylene packing straps to obtain the substratum. The mixed cement paste comprises the following components in percentages by mass: 0.5-1% of epoxy resin, 10-15% of fine sand, 15-20% of shell powder and the balance of cement; and the total mass percentage is 100%. The substratum is made of materials obtained easily, is low in cost and high in strength, and resists stretching and seawater corrosion. By the manufactured substratum, seedlings are collected easily, and the seedling attachment effect is good. Compared with frequently used chlamys farreri, the substratum is better in effect.

The invention belongs to a method for extensively screening scallop SNP in the technical field of the scallop DNA molecular genetic marker, which comprises the following steps that: solution D and phenol-chloroform are firstly used for extracting a plurality of Chlamys farreri RNA, and a ultraviolet specrophotometer is used for measuring the concentration of the mixture of solution D and the phenol-chloroform which are uniformly mixed; a scallop full-length cDNA sample is re-established and comprises the synthesis of a cDNA first chain and the synthesis and augmentation of a cDNA second chain; then homogenization of full-length cDNA is performed, and ultrasonic breaking is performed; then sequence measuring joints are connected, and terminal repair, joint connection and sample augmentation are included; finally a biological software is used for analyze the data to obtain an SNP marker; then an SNP marker to be screened is selected to design a primer; and DNA of individual scallop is extracted to be equivalently mixed to be used as a template, conventional colony sequence measuring is undertaken after the PCR augmentation, and the position point with the occurring frequency of single base difference being 10 percent or more is defined as an SNP marker. The method has safe and reliable principle, simple and controllable flow procedures, reliable running of large-scale screening, excellent effect and strong practicability.

The invention relates to an invitro recombinant expression technology and functional identification of chlamys farreri peptidoglycan recognition protein (Cf-PGRP) gene. Cf-PGRP is provided with an amino acid sequence in a SEQ ID NO.1. The Cf-PGRP is obtained by the invitro recombinant expression technology, and the recombinant protein has broad sterilizing effect, obvious killing effect on Gram positive bacteria and the certain antibacterial effect on Gram negative bacteria and potential application value in aspects of developing broad antibacterial medicines, immune reinforcing agent, feed additive, and the like.

The invention discloses an interspecific hybridization and propagation method for deep sea scallops and chlamys farreri. The interspecific hybridization and propagation method comprises the following steps: a first step of breeding parent scallops: selecting deep sea scallops and chlamys farreri, separating the male and the female of the two scallops respectively, promoting maturity according to a normal parent scallop breeding method and using the mature scallops for cross experiment after synchronously maturing the mature scallops; a second step of laying eggs and performing hybridization: inducing spawning of the male and female groups of the two scallops respectively, fertilizing obtained eggs with conspecific or heterogeneous sperms respectively to obtain pure-bred self-bred progenies of the deep sea scallops and chlamys farreri as well as two hybrid fertilized eggs of female deep sea scallops and male chlamys farreri, female chlamys farreri and male deep sea scallops; hatching the obtained fertilized eggs at 13 to 14 DEG C, observing and measuring the proportion of D type larvas at regular times after the D type larvas appear until the maximum hatching rate is reached; a third step of cultivating scallops, wherein at a larval phase, the average shell height growth speed of the hybrid scallops is 6 to 6.3m per day, and the shell height, the shell length, the shell width and the weight are increased in a cultivating phase. According to the interspecific hybridization and propagation method disclosed by the invention, the deep sea scallops are effectively utilized through interspecific hybridization, so that the scallop breeding species are increased.

The invention relates to a method for screening of chlamys farreri G-type muramidase gene polymorphism markers and the assistant breeding method thereof, belonging to shellfish molecular marker assistant breeding technology in the filed of aquatic organism technology, mainly comprising the steps of: cloning partial sequence of promoter region of a chlamys farreri G-type muramidase gene, preparing disease-resistance population and sensitive population, screening of disease-resistance G-type muramidase gene markers, quickly screening the disease-resistance population and building gene marker assistant breeding technology. According to the invention, promoter region sequence of the chlamys farreri G-type muramidase gene is cloned, and its polymorphism sites are screened. The occurrence frequency of -391 AG individual in disease-resistance population is obviously higher than that in sensitive population, therefore, resistance-related gene marker assistance breeding method is built with -391 AG as chlamys farreri resistance-related G-type muramidase gene marker. The invention has the characteristics of strong pertinence, high breeding efficiency, simple and quick operation, etc., and is suitable for screening of shellfish resistance-related markers and breeding of disease-resistance fine varieties.

The present invention discloses tetraploid preparing process suitable for chlamys Farreri. Natural diploid chlamys Farreri with propagation capacity is fertilized artificially to obtain fertilized egg, the fertilized egg is applied with chemical inducing agent to double the chromosome to obtain tetraploid, and the tetraploid is screened in the constant current process. The present invention utilizes special chromosome operating technology to obtain specific germ plasm resource of chlamys Farreri and has rich material source, and stable tetraploid inducing rate over 30 %.

The invention relates to the cloning of a transforming growth factor-beta (TGF-beta) superfamily type I receptor gene Tgfbrl1 of chlamys farreri in a molecular genetic marker technology, a screening and typing technology of a single nucleotide polymorphism (SNP) locus which is relevant with the weight of adductor muscles in the gene and a method for the application of the gene to the breeding of the high-yield chlamys farreri. A total-length complementary deoxyribonucleic acid (cDNA) sequence of the Tgfbr1 gene of the chlamys farreri is obtained by utilizing a homology-based cloning strategy; an SNP lotus is discovered by blastn comparison, and primers and a probe are designed for the locus; and SNP typing is performed in a natural population of the chlamys farreri by using a high-resolution melting curve technology, and the weight of the adductor muscle of an individual is measured. Statistic analysis displays that the loci c.1815C>T are obviously relevant with the weight of the adductor muscles of the chlamys farreri, and the weight of the adductor muscles of individuals with TT genetypes is obviously higher than that of the adductor muscles of individuals with CC and CT genetypes. In the selective breeding process of the high-yield chlamys farreri, the breeding candidate colonies of the chlamys farreri can be subjected to c.1815C>T typing, and the individuals of which the loci c.1815C>T are TT genetypes are used as a breeding parent preferably by combining typing information of other loci which are relevant with growth properties.

The invention develops a new quick determination method of temperature resistant character indexes ABT of chlamys farreri. According to the method disclosed by the invention, the ABT is calculated through a heart rate HR<11 DEG C> at 11 DEG C, and a calculating formula is as follows: ABT=-0.427*HR<11 DEG C>+33.159 (R2=0.5028). According to the method disclosed by the invention, the temperature resistant character ABT of the chlamys farreri are subjected to standard, low-cost and high-flux determination, an obtained ABT estimating value is used as a basis for breeding parent shellfish in a breeding process. Equipment improvement and determination method improvement are performed on a conventional scallop ABT determining system, according to the quick determination method of the temperatureresistant character indexes ABT of the chlamys farreri developed in the invention, the determination rate is about 1min/piece, and the determination efficiency (about 80 times) of the temperature resistant character indexes ABT of the scallops can be significantly improved. A quick estimating method based on scallop heart rate index pairs ABT is provided for the first time. When the method is adopted, a research base can be provided for breeding of stress resistant varieties of the chlamys farreri and establishing of a resistant index system.

The invention discloses a method for dry preparation of a high-quality chlamys farreri adductor. The method comprises the following steps of cleaning fresh scallop, boiling for 0.5 to 1min in water with temperature of 90 to 100 DEG C, removing shell, skirt and internal organs, impregnating for 60min by a compounding solution of guar gum and xanthan gum, boiling for 1min again in water, cooking the scallop adductor, and enabling salt and complex gum to fully enter the adductor; using a cold air dryer to continuously dry for 28 to 32min, so as to prepare the finished dry scallop. The dry scallop prepared by the method has the advantages that the weight of dry scallop accounts for 25 to 35% of fresh scallop; the color is bright and transparent, the quality is firm and dense, the easiness in cracking and loosing of adductor is avoided, and a uniform and ordered tissue structure is formed; by adopting the cold air drying mode, the sanitation quality can be fully guaranteed.

The invention relates to an ecological, intensive and healthy culture method for scapharca broughtonii and chlamys farreri. The novel shallow sea cage mixed-culture method for scapharca broughtonii and chlamys farreri is characterized by comprising the following first culture step of fry selection, the second culture step of device preparation, the third culture step of fry casting, the fourth culture step of first-time cage overturning, the fifth culture step of second-time cage overturning, the sixth culture step of third-time cage overturning and the seventh culture step of adult scapharca broughtonii and adult chlamys farreri obtaining. By means of the novel shallow sea cage mixed-culture method for scapharca broughtonii and chlamys farreri, original bottom-dwelling scapharca broughtonii is cultured in cages, so that the intensification level of culture is increased, the problems that the death rate in scapharca broughtonii cage culture is high and the culture benefit of chlamys farreri is low are solved, and the comprehensive culture benefit is increased.

The invention discloses a chlamys farreri gene chip for detecting the toxic effect of tetrabisphenol A. The chip comprises eight types of oligonucleotide probes fixed onto a solid phase carrier, the eight types of oligonucleotide probes are selected from eight gene sequences specific to chlamys farreri, and the eight types of oligonucleotide probes are nucleotide sequences indicated by SEQ ID No. 1-8. The chip can be used for detecting gene differential expression relevant information of eight relevant genes of detoxification metabolism of the chlamys farreri under the coercion of the tetrabisphenol A, and a detection method of the chip comprises the steps of preparing a cDNA sample of the chlamys farreri; conducting fluorescence labeling on the cDNA sample; making the cDNA sample obtained after the fluorescence labeling hybridize with the gene chip; scanning hybridization signals for detecting the gene chip, and obtaining the result. By the application of the chip, the high-throughput detection of the gene expression information can be achieved, the chip further has the advantages of being simple and convenient to operate, efficient and reliable in detection result and capable of saving plenty of manpower, material resources and financial resources and the like, and the wide application prospect is achieved.

The invention relates to a special compound feed for Chlamys Farreri and a production method thereof and belongs to the technical field of aquaculture feed. The special compound feed comprises the following ingredients in parts by weight: 10-20 parts of bean meal, 10-15 parts of peanut meal, 5-10 parts of corn flour, 20-30 parts of alfalfa meal, 5-10 parts of dried marine algae powder, 1-5 parts of fish meal, 2-10 parts of shrimp meal, 1-2 parts of compound vitamins and 1-2 parts of special minerals. The processing method of the special compound feed for Chlamys Farreri comprises the following steps: step (1), weighing the raw materials; step (2), mixing the weighed raw materials and performing rough crushing; and step (3), performing ultrafine crushing to the roughly crushed materials. The special compound feed for Chlamys Farreri has scientific formula and the production method is simple in processes. The special compound feed for Chlamys Farreri produced according to the production method has an appropriate calcium-to-phosphorus ratio. Compared with other feeds, the special compound feed for Chlamys Farreri has the effects of obviously promoting growth of the Chlamys Farreri, shortening the culture cycle and improving the anti-adversity ability; moreover, the special compound feed for Chlamys Farreri is also capable of saving marine algae resources and reducing pollution to the water; thus, the special compound feed for Chlamys Farreri has positive promoting effects to the Chlamys Farreri culture industry and is obvious in economic and social benefits.

The invention relates to an expression technology of Chlamys farreri serine protease enzyme inhibitor CfKZSPI gene clone and in-vitro reconstitution. Chlamys farreri serine protease enzyme inhibitor CfKZSPI gene has motif sequence in the sequence table SEQ ID No.1. The invention takes use of the constructed Chlamys farreri cDNA library and adopts RACE technology and in-vitro reconstitution expression technology to study Chlamys farreri serine protease enzyme inhibitor CfKZSPI, and the full-length sequence of cDNA of serine protease enzyme inhibitor CfKZSPI is cloned from Chlamys farreri, and the gene has the sequence provided in the sequence list; and pronucleus reconstitution technology is used to express the recombinant protein with strong trypsin inhibition activity.

The alpha-2 macroglobulin gene of Chlamys Farreri features that it encodes macroglobulin polypeptide of Chlamys Farreri with SEQ ID No. 2 sequence. First, guessmer primer and degenerate primer are made to produce inverse transcriptive PCR, and then, fast cloning in 3' end and 5' end is performed. The alpha-2 macroglobulin gene of Chlamys Farreri is used in culture and development of excellent resistant Chlamys Farreri varieties.

The invention discloses a method for culturing a chlamys farreri trochophore cell line. A small amount of nutrient factors for keeping cell metabolism are added in a special cell culture medium for chlamys farreri trochophore, so that the good growth state of chlamys farreri trochophore larva in vitro culture cells is realized, and the stable subcultring of the cells can be realized along with cell division. The method is simple to operate and low in cost; the complete dissociation of the cells is realized by use of the combination of soaking in a special calcium-free sea-water iso-osmia buffer solution for the chlamys farreri trochophore and a mechanical method, without any digestion by enzymes; the method is little in damage of the chlamys farreri trochophore larva in vitro culture cells, low in cost, high in efficiency and repeatable; a stable marine invertebrate cell in vitro culture method is established and can realize the in vitro multiplication and the subcultring of the cell, the chlamys farreri trochophore cell line is the first continuous cell line of marine shells, and the method has an important guiding significance for cell line establishment of other shells except the chlamys farreri.

The invention relates to a high-efficiency chromosomal mapping method for chlamys farreri genes. First, the preparation of chlamys farreri chromosome is carried out on a glass slide by the method of air-drying; then online primer design and primer synthesis are carried out according to the sequence of the gene to be mapped; the chromosomal mapping of rRNA gene is carried out; the glass slide supporting the chromosome is treated by utilizing a ligase; then formamide denaturalization treatment is carried out to the chromosome; LAMP reaction mixture is added and LAMP reaction in situ is carried out; after the reaction is finished, the glass slide is cleaned and added with a biotin antibody marked by fluorescence for incubation; finally, a propidium iodide anti-fading solution is used for redyeing the chromosome; a stable and clear hybridization signal is used for determining the position of a target gene on the chromosome. The method of the invention causes chlamys farreri gene to fast obtain a plurality of amplification products; the hybridization signal is clear and stable, thus promoting the correctness and specificity of chromosomal mapping. As for the mapping of a single-copy or low-copy gene, the method also has very great application potential.

The invention relates to an aquaculture method of shells, in particular to an inductive agent for inducing marine bivalve mollusk to produce eggs as well as an application method thereof. The application method comprises the following steps: taking cooked egg yolk liquid obtained through bolting-silk treatment as the inductive agent, splashing the inductive agent in a parent shellfish pool uniformly according to the concentration, being 1.0 to 1.5 g/m<3>, of the cooked egg yolk under the conditions that the water temperature is 20 to 30 DEG C, the salinity is 25 to 35 and the pH value is 7.5 to 8.3, treating for 0.5 to 1 hour, performing pool separation according to a siphonage method after the parent shellfishes lay eggs, fully filling the pool with water again, and diluting the concentration of cooked egg yolk. Compared with other stimulation methods, the method has the following advantages: the egg producing efficiency can be increased by 30 to 50 percent and the egg producing efficiency of the parent shellfishes can be effectively improved. The inductive agent can be applied to various marine bivalve mollusk such as hard-shelled clam, clam, short-necked clam, cyclina sinensis gmelin, bay scallop, chlamys farreri and patinopecten yessoensis.

The invention belongs to the technical field of aquaculture, in particular to a rapid cadmium removal agent in bivalve shellfish and a preparation method of the agent. The agent comprises 0.5-3g of an antioxidant, 2-5g of a chelating agent and 0.5-5g of metal salt, wherein the metal salt is zinc sulfate, calcium chloride and ferric chloride added according to the mass ratio of 5: 9: 1 to 4: 10: 1. The removing agent has high removing rate for Chlamys farreri in a short time, does not cause serious secondary pollution, has no obvious toxic effect on human bodies, has low removing cost, is simple and convenient to operate, is beneficial to removing heavy metal cadmium from related shellfish farms, and improves edible safety and economic benefits of shellfish.

The present invention relates to type G lysozyme, and is especially type G lysozyme gene of Chlamys Farreri and its coded protein and cloning method. The type G lysozyme gene of Chlamys Farreri has the base sequence shown in SEQ ID No. 1, and its coded protein has the amino acid sequence shown in SEQ ID No 2. The type G lysozyme gene of Chlamys Farreri the present invention clones has polyadenyl acid tailing signal and polyadenyl acid tail, has important function in immunologic defence of Chlamys Farreri, and may be used in the recombinant expression and gene transfer of lysozyme. The present invention lays foundation for the disease prevention and treatment, gene aided selective breeding and medicine development of Chlamys Farreri.

The invention discloses a net piece feeding method for artificial seedling of adhesion-type dual-shell animals. The feeding method comprises the steps of firstly determining the opportunity for feeding the net pieces, calculating the number of the net pieces required by a culturing pond, designing the feeding line number and the line spacing of the culturing pond, hanging and feeding a net by virtue of a net feeder, and finally arranging the net pieces, so as to finish the whole net feeding process, wherein the main body of the net feeder is of a rod-shaped net frame, a plurality of net teeth are arranged on the net frame, and handles are respectively arranged at two ends of the net frame. The feeding method is applicable to multiple adhesion-type dual-shell animals such as bay scallops, chlamys farreri, scapharca broughtonii and the like. The net piece feeding method has the advantages that the line production can be adopted, the operation steps are simple, lots of labor is not required, each operator is detailed in work division and is skilled in operation, so that the large-scale net piece feeding operation has the characteristics of high efficiency and convenience; besides, the adopted net feeder is simple in structure, easy to produce, low in production cost and convenient to generalize in large scale.