Tetraploid preparing process suitable for chlamys Farreri

A technology of Chlamys farreri and tetraploids, which is applied in the field of tetraploid preparation of Chlamys farreri, can solve problems such as inability to obtain eggs, hindered development of triploid gonads of Chlamys farreri, and inability to complete fertilization of eggs, so as to achieve abundant sources , the effect of stabilizing the induction rate

Inactive Publication Date: 2006-03-01
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

However, the technology of directly preparing tetraploids from diploids is rarely reported at home and abroad.
[0004] The development of triploid gonads of Chlamys farreri is severely hindered, and eggs cannot be obtained by oxytocin triploids, and eggs obtained by manual dissection cannot be fertilized
Therefore, the technique of using triploids to induce tetraploids is not feasible, and it is necessary to explore the preparation method of tetraploids of Chlamys farreri

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Utilize the method of the present invention, prepare the tetraploid of Chlamys farreri, concrete operation is:

[0023] 1) Select 1-year-old diploid scallops with well-developed gonads and brightly colored gonads from natural sea areas;

[0024] 2) Male and female sorting, artificial induction of labor to obtain sperm and eggs, artificial insemination, the ratio of sperm and eggs during insemination is 100:1, obtain fertilized eggs, and adjust the density to 100,000 grains / L;

[0025] 3) At 18°C, add cytochalasin B at a concentration of 0.5 mg / L 15 minutes after fertilization, and treat for 15 minutes;

[0026] 4) Rinse the fertilized eggs with seawater, use a sieve to remove the drug, collect the fertilized eggs, suspend them in seawater again, and incubate them in a conventional way (the incubation density is about 50 / mL);

[0027] 5) Cultivate the induced embryos and larvae according to conventional methods at a water temperature of 18°C ​​until the larvae metamorph...

Embodiment 2

[0030] Utilize the method of the present invention, carry out the polyploid larva sample preparation of Chlamys farreri, concrete operation is:

[0031] 1) Select 2-year-old diploid scallops with well-developed gonads and brightly colored gonads from natural sea areas;

[0032] 2) Male and female sorting, artificial induction of labor to obtain sperm and eggs, artificial insemination, the ratio of sperm and eggs during insemination is 120:1, and fertilized eggs are obtained, and the density is adjusted to 500,000 / L (suspended in seawater) ;

[0033] 3) At 20°C, add cytochalasin B at a concentration of 0.1 mg / L 12 minutes after fertilization, and treat for 20 minutes;

[0034] 4) Rinse the fertilized eggs with seawater, use a sieve to remove the drug, collect the fertilized eggs, suspend them in seawater again, and incubate them in a conventional way (the incubation density is about 50 / mL);

[0035] 5) Cultivate the induced embryos and larvae according to conventional methods...

Embodiment 3

[0038] 1) Select 3-year-old diploid scallops with well-developed gonads and brightly colored gonads from natural sea areas;

[0039] 2) Male and female sorting, artificial induction of labor to obtain sperm and eggs, artificial insemination, the ratio of sperm and eggs during insemination is 100:1, and fertilized eggs are obtained, and the density is adjusted to 100,000 / L (suspended in seawater) ;

[0040] 3) At 18°C, add cytochalasin B at a concentration of 1 mg / L 10 minutes after fertilization, and treat for 15 minutes;

[0041] 4) Rinse the fertilized eggs with seawater, use a sieve to remove the drug, collect the fertilized eggs, suspend them in seawater again, and incubate them in a conventional way (the incubation density is about 50 / mL);

[0042] 5) Cultivate the induced embryos and larvae according to conventional methods at a water temperature of 18°C ​​until the larvae metamorphose into young shellfish; 48 hours after fertilization, flow cytometry analysis shows tha...

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PUM

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Abstract

The present invention discloses tetraploid preparing process suitable for chlamys Farreri. Natural diploid chlamys Farreri with propagation capacity is fertilized artificially to obtain fertilized egg, the fertilized egg is applied with chemical inducing agent to double the chromosome to obtain tetraploid, and the tetraploid is screened in the constant current process. The present invention utilizes special chromosome operating technology to obtain specific germ plasm resource of chlamys Farreri and has rich material source, and stable tetraploid inducing rate over 30 %.

Description

technical field [0001] The invention relates to the induction and cultivation of shellfish polyploids, and in particular provides a tetraploid preparation method suitable for Chlamys farreri. Background technique [0002] Utilizing biotechnology to cultivate excellent cultured species is one of the key issues to be solved urgently in the development of seawater shellfish culture. Triploid shellfish has many excellent production characters, and is a valuable new strain of cultured shellfish. At present, physical and chemical methods are usually used to artificially induce polyploids, and the ploidy rate varies greatly, and it is difficult to reach 100%. Moreover, artificial induction has a great negative impact on the induced objects, such as low survival rate and high proportion of abnormally developed embryos and larvae . Using artificially cultivated tetraploids to hybridize with normal diploids can efficiently obtain triploids with a high magnification rate, which is th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 阙华勇张国范张福绥
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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