Quick determination method of temperature resistant character indexes ABT of chlamys farreri

A fast detection technology for Chlamys farreri, applied in the measurement of pulse rate/heart rate, diagnostic record/measurement, medical science, etc., can solve the problems of lack of temperature-resistant ABT and other problems, and achieve the effect of improving the measurement method and improving the measurement efficiency

Active Publication Date: 2020-06-16
OCEAN UNIV OF CHINA
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AI Technical Summary

Problems solved by technology

Solve the problem of the lack of standardized high-throughput measurement of temperature-resistant traits ABT in the current marine economic shellfish farming process, so that ABT can be used as a trait index, just like body size traits such as shell height, shell length, shell width, and weight. Genome-wide selective breeding of scallop

Method used

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  • Quick determination method of temperature resistant character indexes ABT of chlamys farreri
  • Quick determination method of temperature resistant character indexes ABT of chlamys farreri
  • Quick determination method of temperature resistant character indexes ABT of chlamys farreri

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Embodiment 1

[0039] 1. The connection of the scallop heartbeat detection instrument:

[0040] (1) The interface of Infrared Sensors (CNY-70) is connected to Heartbeatmonitor amplifier (AMP-03) through RJ11 connection;

[0041] (2) Both ends of the Heartbeat monitor amplifier (AMP-03) and PowerLab 16 / 35 sixteen-channel research-type high-speed recording host (including LabChart Pro professional version) are connected to each other by BNC interface;

[0042] (3) PowerLab 16 / 35 sixteen-channel research-type high-speed recording host (including LabChart Pro Professional Edition)

[0043] Connect to the computer via USB (install LabChart v8 for Windows).

[0044] 2. Standardization of scallop heartbeat detection method:

[0045] (1) Remove the attached organisms on the shell of the experimental scallops (N ≥ 18, similar in size, and have different genetic backgrounds), and put them under experimental conditions for 5-7 days to adapt to the experimental environment and stabilize the heartbeat;...

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Abstract

The invention develops a new quick determination method of temperature resistant character indexes ABT of chlamys farreri. According to the method disclosed by the invention, the ABT is calculated through a heart rate HR<11 DEG C> at 11 DEG C, and a calculating formula is as follows: ABT=-0.427*HR<11 DEG C>+33.159 (R2=0.5028). According to the method disclosed by the invention, the temperature resistant character ABT of the chlamys farreri are subjected to standard, low-cost and high-flux determination, an obtained ABT estimating value is used as a basis for breeding parent shellfish in a breeding process. Equipment improvement and determination method improvement are performed on a conventional scallop ABT determining system, according to the quick determination method of the temperatureresistant character indexes ABT of the chlamys farreri developed in the invention, the determination rate is about 1min/piece, and the determination efficiency (about 80 times) of the temperature resistant character indexes ABT of the scallops can be significantly improved. A quick estimating method based on scallop heart rate index pairs ABT is provided for the first time. When the method is adopted, a research base can be provided for breeding of stress resistant varieties of the chlamys farreri and establishing of a resistant index system.

Description

Technical field: [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a rapid determination method for the temperature-resistant trait index ABT of Chlamys farreri. Background technique: [0002] The shellfish industry is an important part of my country's mariculture industry, and the aquaculture production accounts for about 71.09% of the total mariculture production (China Fishery Statistical Yearbook, 2019). Shellfish farming represented by scallops once led the third wave of marine aquaculture in my country and promoted the rapid development of the aquaculture industry. Chlamys farreri is the earliest scallop species cultivated in China, and it belongs to the leading shellfish culture species in the northern coast of my country. Since the end of the 20th century, Chlamys farreri often died on a large scale during the high temperature season in summer, which not only brought huge economic losses to farmers, but also restri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54A61B5/0402A61B5/0205
CPCA61B5/0205A61B5/024A61B2503/40A61B2503/42A01K61/54A61B5/318Y02A40/81
Inventor 邢强朱星海彭程陆维黄晓婷张玲玲包振民
Owner OCEAN UNIV OF CHINA
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