6 results about "Argopecten irradians" patented technology

The application discloses a SNP site, a gene and application thereof related to a fast-growing and high-yield trait of Chlamys hirundo. EPDR1 The site is located at 3,885,288 of chromosome 10 (chr10-3885288) of Chlamys hirundo, is in an intron region of the gene and shows T / C base polymorphism, and has significant correlation with the growth trait of Chlamys hirundo. EPDR1 Experimental results show that different genotypes of the site are significantly related to the shell height trait, and TT and TC genotype individuals show obvious growth advantages. In addition, the experiment further proves that inhibiting the expression of the Chlamys hirundo gene can promote the growth of the Chlamys hirundo. EPDR1 Based on the above finding, the SNP site and the gene where the SNP site is located can be used as a key molecular marker and a key gene for breeding of a new Chlamys hirundo variety with fast growth and high yield, and can effectively improve breeding efficiency and accelerate the breeding process of a good variety.

The present invention provides a method for preparing allodiploid scallops, which is to screen for all-female individuals among the hybrid offspring of Argopecten irradians (♀) and Argopecten purpuratus (♂), fertilize the eggs of the all-female individuals with the sperm of Argopecten irradians, and cultivate the fertilized eggs to obtain allodiploid scallops; the screening is to screen for all-female individuals with a ratio of the nuclear genome derived from Argopecten irradians to the nuclear genome derived from Argopecten purpuratus in the genome higher than 1.5. The method of the present invention is different from the currently mainstream methods of inhibiting the release of polar bodies of diploid fertilized eggs by chemical reagents, salinity adjustment, cold and heat shock, etc., and the method of obtaining triploid offspring by crossing tetraploid and diploid parent scallops, and has opened up a brand-new allodiploid scallop breeding technology.

The invention discloses an SNP (Single Nucleotide Polymorphism) site related to fast-growing and high-yield traits of argopecten irradias, a gene and application of the SNP site and the gene. The loci are located at the 3rd 885th site and the 288 site (chr10-3885288) of the No.10 chromosome of the argopecten irradians, are located in an intron region of an EPDR1 gene, show T / C base polymorphism, and have significant relevance with the growth traits of the argopecten irradians. Experimental results show that different genotypes of the site are obviously related to shell height characters, and TT and TC genotype individuals show obvious growth advantages. In addition, experiments further prove that the growth of the scallops can be promoted by inhibiting the expression of the bay scallop EPDR1 gene. Based on the discovery, the SNP locus and the EPDR1 gene where the SNP locus is located can serve as key molecular markers, the key genes are used for breeding of fast-growing and high-yield new varieties of the argopecten irradians, the breeding efficiency can be effectively improved, and the breeding process of improved varieties is accelerated.

The invention provides a preparation method of high-yield high-temperature-resistant homologous triploid argopecten irradians. The preparation method comprises the following steps: screening parent argopecten irradians with high-temperature-resistant characteristics through a molecular marker-assisted breeding technology; after induced spawning of the parent shellfish, fertilized eggs are treated with cytochalasin B, the fertilized eggs are induced to generate the homologous triploid, the cleavage rate, the hatching rate, the metamorphosis rate and the triploid larva rate are comprehensively considered in the process, and efficient induction of the homologous triploid of the hermaphrodite shellfish is achieved. The high-temperature-resistant molecular marker is developed through SNP sites which are identified through whole genome association analysis and are most remarkably related to the high-temperature-resistant performance of the argopecten irradians, and individuals with high-temperature-resistant genotypes are screened out for spawning and fertilization. Compared with common diploid larvae, the triploid larvae obtained through the method have the higher growth and development speed, and the artificial breeding cost can be greatly reduced.

The present application belongs to the technical field of preparation of excellent commercial seed of economic marine shellfish, and particularly relates to a high-efficiency method for preparing interspecific three-way hybrid of Argopecten genus scallop. The method uses a selected and purified male sterile and female fertile hybrid Argopecten mactrolineatus as a female parent, and a restocked Argopecten purpuratus as a male parent, to produce a "Argopecten mactrolineatus Argopecten purpuratus" three-way hybrid scallop commercial seed with hybrid vigor and outstanding stress resistance. The method can realize large-scale, continuous and efficient hybrid seed production, and has the advantages of simple operation, labor saving, low dependence on personnel, and the like. The hybridization rate is increased from less than 30% in the prior art to more than 90%, and the efficiency of interspecific hybrid seed production and the purity of the seed of the protandrous Argopecten genus scallop are significantly improved.

The application provides a preparation method of high-yield high-temperature-resistant homologous triploid Argopecten irradias, and the Argopecten irradias parent shellfish with high-temperature-resistant characteristics is screened through a molecular marker assisted breeding technology; after the parent shellfish is induced to spawn, the fertilized eggs are treated by using cytochalasin B to induce the generation of homologous triploidy, and the cleavage rate, the hatching rate, the metamorphosis rate and the triploid larva rate are comprehensively considered, so that the high-efficiency induction of the homologous triploid of the hermaphrodite shellfish is realized. The SNP site most significantly related to the high-temperature-resistant performance of the Argopecten irradias is identified through whole genome association analysis, a high-temperature-resistant molecular marker is developed, and the individual with the high-temperature-resistant genotype is screened for spawning and fertilization. The triploid larva obtained through the method has a faster growth and development speed than the common diploid larva, and the artificial breeding cost can be greatly reduced.