72 results about "Argopecten irradians" patented technology

The invention relates to a method for cultivating a new variety of hybridized and backcrossed Argopecten purpuratus. The method is characterized by comprising the following steps of: selecting excellent individuals in a hybridized first filial generation family or group of Argopecten irradians and the Argopecten purpuratus; backcrossing the excellent individuals with the Argopecten purpuratus to establish a backcrossed family or group; selecting hermaphroditic excellent individuals with good representation in the backcrossed family or group; and continuously cultivating at least third generations of self-bred families to obtain the new variety of the hybridized and backcrossed Argopecten purpuratus having the advantages of steady heredity, rapid growth, large individuals and wide temperature tolerance range. According to the method provided by the invention, the new variety can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 30%.

The invention relates to a method for cultivating hybridized and backcrossed marine product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten purpuratus and Argopecten irradians, and synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten irradians; inducing the hybridized first filial generation individuals to spawn by using a conventional shade drying and temperature stimulation method so as to obtain unfertilized eggs; simultaneously, stimulating the Argopecten irradians to spawn and discharge seminal fluid, and filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten irradians; and fertilizing the hybridized first filial generation eggs with the seminal fluid of the Argopecten irradians, cultivating according to a conventional method after hatching germ cells, and thus obtaining the hybridized and backcrossed marine product fries which are above 1.5 cm in shell height and used for commercial cultivation. The hybridized and backcrossed marine product fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed marine product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed marine product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

The invention relates to a method for cultivating hybridized and backcrossed Argopecten product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten irradians and Argopecten purpuratus; synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten purpuratus; inducing the hybridized first filial generation individuals to ovulate by using a conventional shade drying and temperature stimulation method; simultaneously, stimulating the Argopecten purpuratus to spawn and discharge seminal fluid; filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten purpuratus; fertilizing the seminal fluid of the Argopecten purpuratus with the hybridized first filial generation eggs; carrying out larva cultivation and fry intermediate cultivation according to a conventional method after hatching; and thus obtaining the hybridized and backcrossed Argopecten product fries having obvious heterosis. The hybridized and backcrossed marine Argopecten fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed Argopecten product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed Argopecten product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

Provided is a Korea and Mexico gulf scallop hybrid cultivation method. At the first year, good parent scallops are selected, maturation acceleration is performed on the parent scallops, ova of single Korea gulf scallop and sperms of single Mexico gulf scallop are collected and subjected to mixed insemination to establish Korea and Mexico gulf scallop first filial generation families, and a conventional gulf scallop breeding, keeping and offshore cultivation method is adopted to perform breeding; at the second year, good hybridization families are selected according to the phenotypes of the hybridization families obtained at the first year, good-phenotypic-trait individuals are selected from the good hybridization families, self-fertilization is performed for purification after maturation acceleration, and accordingly multiple Korea and Mexico gulf scallop first filial generation inbred families are established; the same method is adopted to continuously perform inbreeding for more than five generations, namely new variety of stable-inheritance, quickly-growing and large-sized scallops wide in temperature resisting range is obtained. The new variety simultaneously has the good advantages of the Korea gulf scallops and the Mexico gulf scallops. In addition, Korea and Mexico gulf scallop hybrids can be bred in most of sea areas in our country, the yield is improved by higher than 30% compared with the yield of Mexico gulf scallops bred in the southern of our country, and the Korea and Mexico gulf scallop hybrid cultivation method has great economic value.

A method for extracting polysaccharide from Argopecten irradians comprises processing raw material, extracting with water, separating, concentrating, precipitating with ethanol and drying, which belongs to the technical field of food science. Specifically, the method comprises pounding Argopecten irradians tissue, oven drying or freeze drying, soaking in a reagent, and refluxing for removing oligosaccharide and pigment; extracting with water as a solvent; centrifuging to obtain a supernatant; performing vacuum rotary evaporation and concentration; adding anhydrous ethanol into the obtained concentrated solution for precipitating polysaccharide when the ethanol reaches a certain of concentration; and drying. The inventive method has high yield. The obtained product has high purity, is easy for further separation and purification, and can be directly used as a product or be processed into tablets and capsules. The invention is of far reaching importance to high-value processing of Argopecten irradians and development of functional food.

The invention relates to a construction method and application of the maintainer line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The maintainer line is Argopecten irradians concentricus strains capable of enabling the generations of the male sterility line of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus to have the characteristics of male sterility and ovum fertility, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are the hybrid generations of purple scallop ova and Argopecten irradians concentricus sperms. The construction method comprises the steps that firstly, a large number of the first filial generations of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are constructed, and male sterile individuals are found out from the first filial generations for breeding of the male sterility line; then sperms of male parent Argopecten irradians concentricus are used for continuous backcross, and the combinations of the male sterility line-maintainer line with all generations of male sterility individuals are respectively bred; finally, the male sterility line parent scallops are produced through hybridization of the male sterility line and the maintainer line, and the maintainer line is used for producing commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus. According to the construction method and application, the maintainer line of the scallops is firstly built in animals, and the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated.

The invention relates to a culture method of Mexico and Korea hybrid scallops. According to the method, in the first year, good seed scallops are selected and are subjected to ripening acceleration, then, ova of a single Mexico gulf scallop and sperms of a single Korea gulf scallop are respectively collected, and are mixed for insemination, a Mexico and Korea hybrid first generation family is built, and conventional gulf scallop fry culture, fry preservation and sea cultivation methods are used for culture; in the second year, good hybrid families are selected according to the expression types of each hybrid family obtained in the first year, individuals with good properties are selected from the good hybrid families, and after the ripening acceleration, the autofertilization is respectively carried out for purification, so a selfing family of a plurality of Mexico and Korea hybrid sub first generations is built; selfing is continuously carried out for more than five generations by the same method, and a new scallop variety with the advantages of stable heredity, high growth speed, great individual size and wide temperature-resistant range can be obtained. The scallops in the variety simultaneously have the excellent characteristics of the Mexico gulf scallops and the Korea gulf scallops, and can be cultured in most sea area in China, the yield is improved by more than 40 percent through being compared with that of the Mexico gulf scallops cultured in south China, and great economic values are realized.

The invention discloses a method for cultivating a new variety of argopecten purpuratus and argopecten irradians concentricus hybrid scallops. The method comprises the following steps that S1, argopecten purpuratus and argopecten irradians concentricus scallops are utilized for colony hybridization, and a mixed colony including a parent selfing colony and a hybrid colony is obtained; S2, after shell color primary selection is carried out, hybrid individuals are identified with molecular biomarkers; S3, selfing purification of argopecten purpuratus and argopecten irradians concentricus hybrid F1 is carried out, wherein the identified hybrid individuals good in expression are selected, signal parent self sperms and eggs are subjected to selfing to set up a selfing family, a hybrid F2 familygood in expression is selected from the selfing family next year, at least three generations are continuously subjected to selfing breeding according to the same method, and the new variety of the argopecten purpuratus and argopecten irradians concentricus hybrid scallops good in character expression is obtained. The new variety of the character argopecten purpuratus and argopecten irradians concentricus hybrid scallops obtained through the method is high in combining ability, fast in growth, high in dressing percentage and bright in shell color, has good hybrid vigor, and can adapt to the high-temperature environment of the southern sea area in summer well.

The invention relates to a method for selectively breeding bay scallop, in particular to a contemporary multipoint composite breeding method for the bay scallop. Continuous shell length head-removed selection is carried out on scallops in different growth periods in the same generation of the bay scallop, the scallop with growth advantage is selected, and further contemporary multipoint compositebreeding is realized. The invention provides a scientific and effective bay scallop fast and efficient breeding method, scale of basic population used for breeding is expanded, reduction of genetic drift and genetic diversity caused by the fact that scale of a breeding group is small in a bay scallop breeding plan is avoided; meanwhile, a multi-time point continuous breeding method is used for improving breeding strength, breeding speed can be improved, and bay scallop breeding efficiency is improved.

The invention relates to a construction method and application of the restorer line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The restorer line is Argopecten irradians concentricus strains capable of enabling the generations of the male sterility line to restore the characteristic of male sterility, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are the hybrid generations of purple scallop ova and Argopecten irradians concentricus sperms. The construction method comprises the steps that excellent individuals in the male sterility line are selected, different varieties of Argopecten irradians concentricus are used for continuous backcross until all generations are hermaphrodite, and the restorer line of the male sterility line is obtained; finally, the restorer line and the corresponding male sterility line are used for hybridization to produce commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus. According to the construction method and application, the restorer line of the male sterility liner is firstly built in animals, the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated, and the yield of the produced hybrid scallops of the purple scallops and the Argopecten irradians concentricus is improved by more than 42 percent relative to that of the Argopecten irradians concentricus in the same year.

The present invention relates to type G lysozyme, and is especially type G lysozyme gene of and its coded protein and cloning method. The type G lysozyme gene of gulf scallop has the base sequence shown in SEQ ID No. 1, and its coded protein has the amino acid sequence shown in SEQ ID No 2. The type G lysozyme gene of gulf scallop the present invention clones has polyadenyl acid tailing signal and polyadenyl acid tail, has important function in immunologic defence of gulf scallop, and may be used in the recombinant expression and gene transfer of lysozyme. The present invention lays foundation for the disease prevention and treatment, gene aided selective breeding and medicine development of gulf scallop.

The invention relates to an establishment method and an application of a violet black hybrid scallop restorer. The restorer is a violet scallop system which enables the male sterile line offspring to restore the male fertile feature, wherein the violet black hybrid scallop is a hybrid offspring of Argopecten irradians concentricus eggs and violet scallop sperms. The establishment method comprises the steps of selecting excellent individuals in the male sterile line, continuously backcrossing different violet scallop sperms, and obtaining the restorer of the male sterile system until all offspring are hermaphrodite; and finally hybridizing the restorer with corresponding male sterile line to produce the violet black hybrid scallop commodity offspring seed. The male sterile restorer is firstly established in animals, the obstacle of autofertilization in the hybridization of the hermaphrodite scallops can be effectively overcome; the annual yield of the produced violet black hybrid scallop is improved by more than 45 percent than that of the Argopecten irradians concentricus.

The invention belongs a breeding technology of shellfishes and particularly relates to a breeding method of cultivating hybridized scallops of purple scallops and bay scallops in the south and raisingthe hybridized scallops in the north in order to achieve large-scale cultivation of the scallops. According to the breeding method, the young hybridized scallops of the purple scallops and bay scallops are cultivated in the south during autumn and then transported to northern sea areas for follow-up raising in next spring. A new hybridized species selected from the first generation of the hybridized scallops of the purple scallops and bay scallops for breeding is adopted as the breeding scallops, the young scallops are cultivated in the south during autumn and then transported to the northernsea areas for follow-up raising, and therefore the commercial scallops with large specification are cultivated. Through establishment of a breeding pattern, the breeding time of the hybridized scallops of the purple scallops and bay scallops is prolonged, the breeding range is expanded, the specification of the bred scallops can be effectively increased, the value of scallop products is higher, and the quality and safety of the scallop products are effectively ensured. The yield of high-quality marine protein is increased, the income of fishermen is increased, and a new way to healthy sustainable development of the scallop breeding industry is created.

The invention relates to a building method and application of an Argopecten irradians irradians and Argopecten purpuratus hybrid scallop maintainer line. The maintainer line allows the corresponding male sterility line offspring of the corresponding Argopecten irradians irradians and Argopecten purpuratus hybrid scallop to have male sterile and egg fertile features. The Argopecten irradians irradians and Argopecten purpuratus hybrid scallop is the hybrid offspring of the eggs of the Argopecten irradians irradians and the sperms of the Argopecten purpuratus. The method includes: building a large amount of Argopecten irradians irradians and Argopecten purpuratus hybrid scallop first filial generation families, and finding male sterile individuals, for male sterility line breeding, in the families; using the sperms of male parent Argopecten purpuratus for continuous back crossing, and respectively breeding a male sterility line-maintainer line combination with all the offspring being male sterile individuals; using the male sterility line to hybridize with the retainer line to produce male sterility line parent scallops, and using the male sterility line parent scallop produce Argopecten irradians irradians and Argopecten purpuratus hybrid scallop commercial fry. The method has the advantages that the scallop retainer line is built in animals for the first time, and the barrier of self-fertilization of the hermaphrodite scallops during hybridization is overcome effectively.