32results about How to "Improve seed selection efficiency" patented technology

The invention provides application of an SNP molecular marker in detecting the skin color character of a black-bone chicken. The nucleotide sequence of the molecular marker is shown as SEQ ID No.1, and the base mutation type at the 171st bp is A / G. The invention further provides a primer sequence for detecting an SNP locus and a PCR-RFLP method. The genotype of the locus can be distinguished based on the difference of an electrophoretic band after DraI enzyme digestion, and then the homozygosis and heterozygosis of a sample Fm gene can be judged. If the 342 bp band appears, the sample Fm gene is a GG homozygosis-based gene; if the two bands of 171 bp and 342 bp appear, the sample Fm gene is an AG heterozygosis-based gene; if the 171 bp band appears, the sample Fm gene is an AA recessive-homozygosis-based gene. By means of the SNP molecular marker and the detection method, the individual genotype of the black-bone chicken can be judged in the chickling stage, early selection and early elimination of the skin color character can be achieved, and thus significant practical value can be achieved on improving selection efficiency and saving breeding cost.

The invention discloses a method for the growth traits of soft-shelled soft-shelled turtle MSTN Gene molecular marker selection method, that is in soft-shelled soft-shelled turtle MSTN A SNP site on the first intron sequence in the gene, starting from the start codon, is T2194C. Screening out the genes closely related to growth traits in soft-shelled turtle populations MSTN The breeding marker of the gene, and screening and constructing the breeding population of soft-shelled turtle with the breeding marker in the breeding population of Chinese soft-shelled turtle. This method can improve the accuracy of selection of growth traits of Soft-shelled Soft-shelled Turtle, laying the foundation and providing reference for molecular marker-assisted breeding of Soft-shelled Soft-shelled Turtle.

The invention discloses a SNP marker related to the sweet-sour flavor traits of peach fruit and its application. The present invention provides the application of the g.1495589 site genotype detection material of the peach tree genome to be tested in identifying or assisting in identifying whether the fruit of the peach tree to be tested is an acidic phenotype; the g.1495589 site is 5 Chromosome No. 1495589 or Sequence 1 No. 601 Nucleotide. Based on the existing 284 sequencing and chip SNP marker data of peaches, the present invention carried out correlation analysis with phenotypic traits, and successfully located a sweet-acid-related SNP marker on chromosome 5, compared with the previous RAPD , AFLP, and SSR markers can increase the prediction accuracy to more than 85%, making the prediction result more reliable. Prediction of individual sweet-acid phenotypes at the seed stage improves selection efficiency and saves breeding costs.

The invention discloses an auxiliary seed selection device for agricultural seeders with a classified storage structure, which comprises a casing and a driving motor. The closing mechanism is connected with the side wall of the housing, and the inner wall of the housing is fixedly connected with four equidistant vertically arranged storage cylinders, the upper side of the storage cylinders is connected with a feeding hopper, and the upper end of the feeding hopper passes through the cover plate and Extend upwards. The present invention can drive the box body to shake by arranging the transmission mechanism, the shaking mechanism and the engaging mechanism, and the shaking of the box body drives the fourth spring to stretch, and the wedge-shaped block on the box body can be driven to continue contact with the fourth spring due to the restoring force of the fourth spring. The cams touch each other, so that the box body can shake left and right, so as to achieve the function of screening seeds, three layers of screens, so that seeds of multiple specifications can be screened at one time, and seeds of different specifications can be classified and stored, so that the seed selection to raise efficiency.

The invention discloses a SNP marking detection associated with growth trait of haliotis diversicolor and an application. The molecular marker is gene sequence shown as SEQ ID NO.1. The invention further discloses a primer AMY-FP and a primer AMY-EP for detecting the molecular marker; the primer AMY-FP is the sequence shown as SEQ ID NO.2, and the primer AMY-RP is the sequence shown as SEQ ID NO.3. The SNP site obtained through molecular marking, primer, and selection is applied to the selection and breeding of haliotis diversicolor. A parent is selected by using the primer and the method; the growth rate of the later breeding generations is obviously higher than the unselected control group; the molecular marker and the primer can efficiently, rapidly and simply be applied to the selection and breeding of haliotis diversicolor.

The invention relates to the technical field of breeding screening and in particular relates to a cucumber female line induced screening method. The method comprises the following steps: seeding, germinating after seeding, flattening the cotyledon to form four leaves and one core of a young seedling, keeping the temperature of 28 DEG C for three hours in the daytime, keeping the temperature of 32 DEG C for 8-10 hours in the daytime, and keeping the temperature of 28 DEG C for three hours; keeping the temperature of 26 DEG C for 4-6 hours at night, and keeping the temperature of 23 DEG C for 4-6 hours; keeping the illumination for 14-16 hours after the cotyledon is flattened to form four leaves and one core of the young seedling, carrying temperature treatment and light treatment synchronously, wherein the light source is formed by the combination of a 100W xenon lamp with the color temperature being 6000K and a 100W bromine tungsten lamp; ventilating for hardening seedlings, performing field planting, performing field management, and removing plants with male flowers of less than 10 sections after the cucumber has ten leaves and one core. According to the method, female plants can be screened and identified under the condition that the female genetic regularity is not required to be known, the pseudo female plants can be effectively removed, and the seed selection efficiency of female materials is improved.