32results about How to "Improve seed selection efficiency" patented technology

The invention provides application of an SNP molecular marker in detecting the skin color character of a black-bone chicken. The nucleotide sequence of the molecular marker is shown as SEQ ID No.1, and the base mutation type at the 171st bp is A / G. The invention further provides a primer sequence for detecting an SNP locus and a PCR-RFLP method. The genotype of the locus can be distinguished based on the difference of an electrophoretic band after DraI enzyme digestion, and then the homozygosis and heterozygosis of a sample Fm gene can be judged. If the 342 bp band appears, the sample Fm gene is a GG homozygosis-based gene; if the two bands of 171 bp and 342 bp appear, the sample Fm gene is an AG heterozygosis-based gene; if the 171 bp band appears, the sample Fm gene is an AA recessive-homozygosis-based gene. By means of the SNP molecular marker and the detection method, the individual genotype of the black-bone chicken can be judged in the chickling stage, early selection and early elimination of the skin color character can be achieved, and thus significant practical value can be achieved on improving selection efficiency and saving breeding cost.

The invention discloses a breeding method capable of improving the egg yield of muscovy ducks. The breeding method comprises the following steps of early-stage primary election, wherein a high-qualitypopulation are selected in young ducks according to the body-size healthy and strong degree in proportion; first-time egg laying selection, wherein according to the whole-period egg production numberof the previous generation and family information, the breeding values of individuals in the current generation are calculated, and high-egg-yield and high-breeding-value male and female ducks are selected and remain in proportion; egg production determination, wherein when the female ducks start laying, the number of the qualified eggs laid by each female duck individual is recorded; second-timeegg laying selection, wherein according to the egg yield of the female ducks in the current generation and the genealogy information, high-egg-yield and high-breeding-value male ducks and female ducks are selected and remain in proportion; family building, a full-sib family is built by the male ducks and the female ducks in proportion, the next generation is bred, and the above steps are repeated. The breeding method has the advantages that the contradictions between subculture and egg laying selection are well solved, genetic contributions on the egg yield in the later egg laying period aretaken into account, the breeding efficiency is improved, and improvement of economic benefits is facilitated.

The invention discloses a method for the growth traits of soft-shelled soft-shelled turtle MSTN Gene molecular marker selection method, that is in soft-shelled soft-shelled turtle MSTN A SNP site on the first intron sequence in the gene, starting from the start codon, is T2194C. Screening out the genes closely related to growth traits in soft-shelled turtle populations MSTN The breeding marker of the gene, and screening and constructing the breeding population of soft-shelled turtle with the breeding marker in the breeding population of Chinese soft-shelled turtle. This method can improve the accuracy of selection of growth traits of Soft-shelled Soft-shelled Turtle, laying the foundation and providing reference for molecular marker-assisted breeding of Soft-shelled Soft-shelled Turtle.

The invention discloses a SNP marker related to the sweet-sour flavor traits of peach fruit and its application. The present invention provides the application of the g.1495589 site genotype detection material of the peach tree genome to be tested in identifying or assisting in identifying whether the fruit of the peach tree to be tested is an acidic phenotype; the g.1495589 site is 5 Chromosome No. 1495589 or Sequence 1 No. 601 Nucleotide. Based on the existing 284 sequencing and chip SNP marker data of peaches, the present invention carried out correlation analysis with phenotypic traits, and successfully located a sweet-acid-related SNP marker on chromosome 5, compared with the previous RAPD , AFLP, and SSR markers can increase the prediction accuracy to more than 85%, making the prediction result more reliable. Prediction of individual sweet-acid phenotypes at the seed stage improves selection efficiency and saves breeding costs.

The invention discloses a breeding method of a glossy cucumber female line. The method comprises the following steps: hybridizing the glossy cucumber germ plasm with female-line cucumber germ plasm to obtain a generation 1; performing backcrossing by taking the glossy cucumber germ plasm as a recurrent parent to obtain backcrossed 1st-generation seeds, namely a generation 2; in a generation 3, selecting male inducing sister cross in a nursery separation way for polymerization of glossy character and female character, and selecting and remaining seeds; and after that, performing male inducing selfing, purifying the glossy female line in an obvious nursery and a seed selection nursery, and performing selfing of 2-3 generations to obtain the glossy cucumber female line. According to the invention, in the generation 3, through the male inducing sister cross in a nursery separation way, the contradiction between the seed selection and the seed remaining through the polymerization of glossy character and female character is effectively solved; and moreover, the early generation effectively maintains more excellent genes to facilitate later selection of a selfing system with good comprehensive characters.

The invention discloses a kind of corn seed selection and seed dressing equipment, which includes a feeding system, a photodetection system, a screening system, a moving system, a spraying system, a drying system, a coating system, and a solid seed dressing system; During the seeding process, the damage to the corn seed material is minimal, and the skin of the seed material will not be damaged. The loss rate of raw materials is low, the seed selection effect is good, and the seed selection efficiency is greatly improved. The equipment of the present invention can not only detect whether the outer structure of the corn seed material is damaged, but also detect and identify the color and overall plumpness of the corn seed material, so as to realize the effective detection of whether the seed material itself has diseases. The equipment can realize the double seed dressing process of the corn seed material, the seed dressing effect is uniform, and the use effect of the seed material after seed dressing is good, which eliminates the burden of the second seed dressing on the farmer and effectively prevents the farmer from doing the seed dressing operation by himself Adverse consequences of non-compliance.

The invention discloses an auxiliary seed selection device for agricultural seeders with a classified storage structure, which comprises a casing and a driving motor. The closing mechanism is connected with the side wall of the housing, and the inner wall of the housing is fixedly connected with four equidistant vertically arranged storage cylinders, the upper side of the storage cylinders is connected with a feeding hopper, and the upper end of the feeding hopper passes through the cover plate and Extend upwards. The present invention can drive the box body to shake by arranging the transmission mechanism, the shaking mechanism and the engaging mechanism, and the shaking of the box body drives the fourth spring to stretch, and the wedge-shaped block on the box body can be driven to continue contact with the fourth spring due to the restoring force of the fourth spring. The cams touch each other, so that the box body can shake left and right, so as to achieve the function of screening seeds, three layers of screens, so that seeds of multiple specifications can be screened at one time, and seeds of different specifications can be classified and stored, so that the seed selection to raise efficiency.

The invention discloses a SNP marking detection associated with growth trait of haliotis diversicolor and an application. The molecular marker is gene sequence shown as SEQ ID NO.1. The invention further discloses a primer AMY-FP and a primer AMY-EP for detecting the molecular marker; the primer AMY-FP is the sequence shown as SEQ ID NO.2, and the primer AMY-RP is the sequence shown as SEQ ID NO.3. The SNP site obtained through molecular marking, primer, and selection is applied to the selection and breeding of haliotis diversicolor. A parent is selected by using the primer and the method; the growth rate of the later breeding generations is obviously higher than the unselected control group; the molecular marker and the primer can efficiently, rapidly and simply be applied to the selection and breeding of haliotis diversicolor.

The invention relates to the technical field of breeding screening and in particular relates to a cucumber female line induced screening method. The method comprises the following steps: seeding, germinating after seeding, flattening the cotyledon to form four leaves and one core of a young seedling, keeping the temperature of 28 DEG C for three hours in the daytime, keeping the temperature of 32 DEG C for 8-10 hours in the daytime, and keeping the temperature of 28 DEG C for three hours; keeping the temperature of 26 DEG C for 4-6 hours at night, and keeping the temperature of 23 DEG C for 4-6 hours; keeping the illumination for 14-16 hours after the cotyledon is flattened to form four leaves and one core of the young seedling, carrying temperature treatment and light treatment synchronously, wherein the light source is formed by the combination of a 100W xenon lamp with the color temperature being 6000K and a 100W bromine tungsten lamp; ventilating for hardening seedlings, performing field planting, performing field management, and removing plants with male flowers of less than 10 sections after the cucumber has ten leaves and one core. According to the method, female plants can be screened and identified under the condition that the female genetic regularity is not required to be known, the pseudo female plants can be effectively removed, and the seed selection efficiency of female materials is improved.

A disclosed mechanical sugarcane seed selection mechanism comprises a seed-selection water tank, a seed-selection liquid, a seed conveying device and a non-seed conveying device; the seed-selection liquid is arranged in the seed-selection water tank; one end of the seed conveying device is arranged at the bottom at one side in the seed-selection water tank, and the conveying direction is arranged along one side of the seed-selection water tank with a long-strip-shaped structure; and the non-seed conveying device is arranged at the upper part of the seed-selection water tank and below the liquid level of the seed-selection liquid, and the conveying direction is opposite to the conveying direction of the seed conveying device. By employing the mechanism, automatic seed selection operation can be performed on sugarcane germination segment and non-germination segment, the seed selection efficiency is improved, and subsequent sowing efficiency is correspondingly improved. Also, a disinfectant and a germination-accelerating matter with a certain ratio are added into the seed-selection water tank, the emergence rate of sugarcane is improved, and thus mechanization is realized, sugarcane plantation difficulty is reduced, labor intensity is mitigated and cost is reduced. The mechanism possesses the characteristics of simple structure, convenient usage and high practicality.