A SNP marker related to sweet-sour flavor traits of peach fruit and its application

A fruit and acidic technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of high cost, long breeding cycle, low accuracy rate, etc.

Active Publication Date: 2021-08-20
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the breeding of peaches is mainly to produce new varieties by conventional hybridization, and it takes 3-4 years after planting to bloom and bear fruit. The breeding cycle is long and the cost is high.
In recent years, RAPD, AFLP, SSR and other molecular marker-assisted breeding related to sweet and sour flavor traits have been developed, but limited by the number of materials, marker density, research methods and many other factors, resulting in low detection accuracy

Method used

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  • A SNP marker related to sweet-sour flavor traits of peach fruit and its application
  • A SNP marker related to sweet-sour flavor traits of peach fruit and its application
  • A SNP marker related to sweet-sour flavor traits of peach fruit and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Acquisition of SNP markers related to sweet-sour flavor traits of peach fruit

[0039] figure 1 It is the acquisition process of SNP markers related to the sweet-sour flavor traits of peach fruit.

[0040] The experimental materials used in the present invention come from the national fruit tree germplasm Beijing peach and strawberry orchard resource groups.

[0041] In this experiment, a total of 192 peach trees in the resource group were selected, and the phenotypes were identified according to the acid coverage of the fruit surface, including 91 sour peaches (fruit acidic phenotype) and 101 non-sour peaches (fruit non-acidic phenotype) .

[0042] The acidic phenotype of the fruit is that the total acid content of the fruit is greater than or equal to 0.4%, and the pH value of the fruit is less than or equal to 4;

[0043] The non-acidic phenotype of the fruit is that the total acid content of the fruit is less than 0.4%, and the pH value of the fruit is...

Embodiment 2

[0090] Example 2. Application of SNP marker g.1495589 in identifying sweet-sour flavor traits of peach fruit

[0091] 1. Extract the genomic DNA of the sample to be tested

[0092] Collect 262 peach young leaf tissues whose fruit acidic phenotypes and non-acidic phenotypes have been identified, place them in tinfoil, and extract each peach tree genomic DNA, the same as the extraction method in Example 1, to obtain the genome of each sample DNA.

[0093] 2. Genotyping of SNP marker loci

[0094] The genomic DNA of each peach tree mentioned above was used to determine the genotype of the peach genome 170K SNP (Affymetrix, Affymetrix, USA) on the Axiom gene chip of the GeneTitan platform according to the company's standard procedure, and the chip signal was analyzed using the SNPolisher package and Genotyping ConsoleTM of Affymetrix Software (GTC) software package clustering is carried out genotyping, determines the genotype of g.1495589 according to the effect of clustering, a...

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Abstract

The invention discloses a SNP marker related to the sweet-sour flavor traits of peach fruit and its application. The present invention provides the application of the g.1495589 site genotype detection material of the peach tree genome to be tested in identifying or assisting in identifying whether the fruit of the peach tree to be tested is an acidic phenotype; the g.1495589 site is 5 Chromosome No. 1495589 or Sequence 1 No. 601 Nucleotide. Based on the existing 284 sequencing and chip SNP marker data of peaches, the present invention carried out correlation analysis with phenotypic traits, and successfully located a sweet-acid-related SNP marker on chromosome 5, compared with the previous RAPD , AFLP, and SSR markers can increase the prediction accuracy to more than 85%, making the prediction result more reliable. Prediction of individual sweet-acid phenotypes at the seed stage improves selection efficiency and saves breeding costs.

Description

technical field [0001] The invention relates to the field of biotechnology, and belongs to the fields of peach molecular breeding and biotechnology, in particular to a SNP marker related to the sweet-sour flavor traits of peach fruit and its application. Background technique [0002] Peach (Prunus persica) fruit is rich in protein, sugar, dietary fiber, vitamins and other nutrients, and is deeply loved by consumers. The sweet and sour flavor of peach fruit is one of the important economic traits of peaches. According to the quality, it is mainly divided into sour and non-sour peaches. Peach originated in China, so the types and quantity of peach resources in our country are much higher than other countries, but the varieties with high economic value are all bred varieties, so the selection of new varieties is to improve the quality and output of peaches in our country important influencing factors. At present, the breeding of peaches is mainly to produce new varieties by c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 谢华徐摇光任飞于洋
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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