The invention discloses a kit and system for prognosis prediction of a metastatic colorectal cancer and relates to the field of biomedicine. The kit comprises a reagent for detecting the expression level of a target gene, wherein the target gene is selected from one or more genes in the group consisting of ACOT11, C12orf45, CHDH, COX17, CTNNB1, CYP2S1, DDTL, DUSP18, FAM221A, FGFR4, KLC4, LARS2, PFDN6, SLC27A3 and TNFRSF11A, the expression levels of the target genes are detected by using the kit, the prognosis condition of the metastatic colorectal cancer can be stably predicted according to the detection result, the prediction result is reliable, the kit has a clinical application prospect, and a new prediction thought or strategy is provided for the prognosis of the metastatic colorectal cancer.