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Apple seed selection method based on bud mutation

A sprouting and apple technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of difficult seed selection, heavy workload, and long time consumption, so as to shorten the breeding cycle and improve accuracy Effect

Inactive Publication Date: 2015-06-17
SHANDONG INST OF POMOLOGY
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0002] As an important fruit, apples are widely eaten by people, and a large number of apples are produced from bud varieties. Therefore, the method of apple selection based on bud mutation is an important method for apple breeding. In the method of seed selection for budding apples, the suspected mutated materials must be identified by high-level grafting to determine that the mutated materials are buds, and then the decorative materials can be removed. The process of apple seed selection takes a long time, occupies a lot of land, and requires a lot of work It consumes a lot of manpower, financial resources and material resources, has high requirements for experimental conditions and personnel, and is greatly affected by the natural environment, making selection difficult and breeding efficiency low

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  • Apple seed selection method based on bud mutation
  • Apple seed selection method based on bud mutation
  • Apple seed selection method based on bud mutation

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Embodiment Construction

[0066] The present invention will be further described below in conjunction with the examples, and the following examples are intended to illustrate the present invention rather than further limit the present invention. First embodiment of the present invention, its steps are:

[0067] a. Primary election

[0068] Unearth variation: Excavate suspected sprout samples in the apple orchard and select control samples, register and number the suspected sprout samples and control samples and mark them with obvious differences;

[0069] Analysis of variation: SSAP molecular identification method is used to analyze suspected budding samples and control samples to determine whether the mutant sample is a budding sample or a modified sample;

[0070] b. Multiple choice

[0071] Expansion of bud mutation resources: Graft bud mutation samples and control samples to dwarf apple stock respectively, and then colonize them in seed selection gardens for cultivation;

[0072] Strain evaluati...

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Abstract

An apple seed selection method based on bud mutation comprises the following steps: judging whether a discovered suspected bud mutation sample is a bud mutation sample or a modification sample by an SSAP molecular identification method; performing the bud mutation resource propagation of the bud mutation sample to obtain a strain; and evaluating the fruit quality and economic characters of the strain to judge whether the bud mutation sample belongs to a new variety. According to the method, whether the suspected bud mutation sample is the bud mutation sample or the modification sample is judged directly by analyzing the suspected bud mutation apple sample by the SSAP molecular identification method, but is not judged by planting and grafting, so that the breeding cycle of apple seed selection is shortened, and the accuracy of apple seed selection is increased.

Description

1. Technical field [0001] The invention relates to a method for selecting apple seeds, in particular to a method for selecting apple seeds based on budding. 2. Background technology [0002] As an important fruit, apples are widely eaten by people, and a large number of apples are produced from bud varieties. Therefore, the method of apple selection based on bud mutation is an important method for apple breeding. In the method of seed selection for budding apples, the suspected mutated materials must be identified by high-level grafting to determine that the mutated materials are buds before removing the decorative materials. It consumes a lot of manpower, financial resources and material resources, has high requirements on experimental conditions and personnel, is greatly affected by the natural environment, is difficult to select, and has low breeding efficiency. 3. Contents of the invention [0003] In order to overcome above-mentioned technical shortcoming, the object...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13
Inventor 何平李林光李慧峰王海波
Owner SHANDONG INST OF POMOLOGY
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