Transforming growth factor-beta (TGF-beta) type I receptor gene of chlamys farreri and single nucleotide polymorphism (SNP) locus of TGF-beta type I receptor gene

A Chlamys farreri, site technology, applied in the field of high-yielding scallop breeding, can solve the problems of few genes or gene sites, and no TGF-β signaling pathway receptor gene report.
CN102899330BInactive Publication Date: 2014-06-18OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2014-06-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the cloning of a transforming growth factor-beta (TGF-beta) superfamily type I receptor gene Tgfbrl1 of chlamys farreri in a molecular genetic marker technology, a screening and typing technology of a single nucleotide polymorphism (SNP) locus which is relevant with the weight of adductor muscles in the gene and a method for the application of the gene to the breeding of the high-yield chlamys farreri. A total-length complementary deoxyribonucleic acid (cDNA) sequence of the Tgfbr1 gene of the chlamys farreri is obtained by utilizing a homology-based cloning strategy; an SNP lotus is discovered by blastn comparison, and primers and a probe are designed for the locus; and SNP typing is performed in a natural population of the chlamys farreri by using a high-resolution melting curve technology, and the weight of the adductor muscle of an individual is measured. Statistic analysis displays that the loci c.1815C>T are obviously relevant with the weight of the adductor muscles of the chlamys farreri, and the weight of the adductor muscles of individuals with TT genetypes is obviously higher than that of the adductor muscles of individuals with CC and CT genetypes. In the selective breeding process of the high-yield chlamys farreri, the breeding candidate colonies of the chlamys farreri can be subjected to c.1815C>T typing, and the individuals of which the loci c.1815C>T are TT genetypes are used as a breeding parent preferably by combining typing information of other loci which are relevant with growth properties.
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Description

technical field

[0001] The invention belongs to the technical field of molecular genetic marker-assisted breeding, and relates to the cloning of the TGF-β I type receptor gene Tgfbr1 of Chlamys farreri, the screening and typing technology of SNP sites related to adductor muscle weight in the gene, and its application in high-yield scallops Methods used in breeding. Background technique

[0002] Transforming growth factor β (transforming growth factor β, TGF-β) signaling pathway plays an important role in biological processes such as cell proliferation, differentiation and growth. TGF-β ligands transmit signals to the nucleus by binding to TGF-β type I and type II receptors, as well as downstream intracellular signaling molecules, and regulate the expression of target genes. The activation of type I receptors is considered to be an important part of TGF-β signal transmission. Studies in mammals have shown that the Tgfbr1 gene is related to cell growth and proliferation, and...

Claims

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