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Cucumber female line induced screening method

A screening method and technology for female lines are applied in the field of induction and screening of female cucumber lines, and achieve the effects of strong density controllability, uniform nutrition in seedling stage, and clean and non-toxic environment.

Inactive Publication Date: 2013-07-10
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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Problems solved by technology

But the efficiency of seed selection is sometimes unsatisfactory

Method used

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  • Cucumber female line induced screening method

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Embodiment Construction

[0025] In order to better understand the present invention, the following will be further described in conjunction with specific examples.

[0026] The South China type female line M16 bred by the unit itself was crossed with the North China type hermaphroditic inbred line X8 and then backcrossed for one generation to obtain an MC population. All female offspring were selected from the backcross population.

[0027] (1) Randomly select 300 MC seeds, soak the seeds in warm soup at 55°C, and keep the water temperature for 2-3 hours when the water temperature drops to 30°C, remove and drain, soak the seeds in a 600-fold multinin solution for 20 minutes, wash them with tap water, and store them at 30°C After 12 hours, the seeds are sown after the seeds are white.

[0028] The sowing greenhouse requires air conditioners that can heat up and cool down, temperature controllers, light control equipment, and supplementary lights.

[0029] Disinfection treatment: Disinfect the greenhou...

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Abstract

The invention relates to the technical field of breeding screening and in particular relates to a cucumber female line induced screening method. The method comprises the following steps: seeding, germinating after seeding, flattening the cotyledon to form four leaves and one core of a young seedling, keeping the temperature of 28 DEG C for three hours in the daytime, keeping the temperature of 32 DEG C for 8-10 hours in the daytime, and keeping the temperature of 28 DEG C for three hours; keeping the temperature of 26 DEG C for 4-6 hours at night, and keeping the temperature of 23 DEG C for 4-6 hours; keeping the illumination for 14-16 hours after the cotyledon is flattened to form four leaves and one core of the young seedling, carrying temperature treatment and light treatment synchronously, wherein the light source is formed by the combination of a 100W xenon lamp with the color temperature being 6000K and a 100W bromine tungsten lamp; ventilating for hardening seedlings, performing field planting, performing field management, and removing plants with male flowers of less than 10 sections after the cucumber has ten leaves and one core. According to the method, female plants can be screened and identified under the condition that the female genetic regularity is not required to be known, the pseudo female plants can be effectively removed, and the seed selection efficiency of female materials is improved.

Description

technical field [0001] The invention relates to the technical field of breeding and screening, in particular to a method for inducing and screening cucumber female lines. Background technique [0002] Cucumber is one of the important vegetable crops in China, and its output and output value are in the forefront of vegetable crops. At present, cucumbers have been cultivated all year round, which plays an important role in the balanced supply of vegetables. [0003] In recent years, with the introduction of female varieties from Europe, America and Japan, the ecological types of cucumber varieties in my country have been enriched. They generally have the advantages of strong parthenocarpic ability, good early maturity, and high yield potential. Breeding provides excellent germplasm. Making full use of foreign excellent female germplasm resources to create North China female germplasm suitable for Chinese consumption habits and applying it to production is the current focus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06
Inventor 曹齐卫孙小镭段乃彬王伟张卫华王志峰
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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