A detection of SNP markers associated with the growth traits of Abalone and its application
A technology for growth traits and nine-hole abalone, which is applied in the field of aquatic animal genetics and molecular marker-assisted breeding, can solve the problems of inhibiting the healthy development of the abalone industry, not paying attention to the selection of parent abalone and the improvement of seedling breeding technology, and the decline of variety germplasm
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[0034]Example: Correlation analysis of growth traits-related SNP sites 18C>G, 299C>T in the α-amylase gene of Abalone abalone, and its application in the breeding of high-yield Abalone abalone, according to the following steps :
[0035] (A) Genomic DNA extraction of Abalone;
[0036] (B) Screening of SNP sites of Abalone α-amylase gene;
[0037] (C) Locus 18C>G, 299C>T typing;
[0038] (D) Correlation analysis between 18C>G, 299C>T genotypes and growth traits;
[0039] (E) The method of site 18C>G, site 299C>T assisting in the selection of high-yielding Abalone abalone.
[0040] The specific operation is as follows:
[0041] (A) DNA extraction from Abalone abalone.
[0042] Take about 0.2 g of adductor muscle of Abalone, add 500 μL 1×TE lysate, cut it into pieces, add 50 μL 10% SDS, 7 μL proteinase K (20 mg / mL) successively, and lyse at 37°C for 30 minutes, then adjust to 55°C for lysing 1 -2h, until the lysate is clear; add 500 μL of phenol / chloroform / isoamyl alcohol s...
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