SNP marking detection associated with growth trait of haliotis diversicolor and its application

A technology for growth traits and nine-hole abalone, which is applied in the fields of aquatic animal genetics and molecular marker-assisted breeding, can solve the problems of frequent occurrence of diseases, inhibiting the healthy development of the abalone industry, and economic loss of the nine-hole abalone breeding industry.

Inactive Publication Date: 2015-09-02
GUANGDONG OCEAN UNIVERSITY +1
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AI Technical Summary

Problems solved by technology

However, with the rapid development of the abalone aquaculture industry, the occurrence of diseases during the seedling and growing period has become more and more frequent
In addition, in the process of abalone breeding, the selection of parental abalone and the improvement of seedling technology are not paid attention to, resulting in the inbreeding of abalone and the continuous decline of species germplasm, which has caused huge economic losses to the abalone aquaculture industry and seriously inhibited the healthy development of my country's abalone industry.

Method used

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  • SNP marking detection associated with growth trait of haliotis diversicolor and its application
  • SNP marking detection associated with growth trait of haliotis diversicolor and its application
  • SNP marking detection associated with growth trait of haliotis diversicolor and its application

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Embodiment

[0034]Example: Correlation analysis of growth traits-related SNP sites 18C>G, 299C>T in the α-amylase gene of Abalone abalone, and its application in the breeding of high-yield Abalone abalone, according to the following steps :

[0035] (A) Genomic DNA extraction of Abalone;

[0036] (B) Screening of SNP sites of Abalone α-amylase gene;

[0037] (C) Locus 18C>G, 299C>T typing;

[0038] (D) Correlation analysis between 18C>G, 299C>T genotypes and growth traits;

[0039] (E) The method of site 18C>G, site 299C>T assisting in the selection of high-yielding Abalone abalone.

[0040] The specific operation is as follows:

[0041] (A) DNA extraction from Abalone abalone.

[0042] Take about 0.2 g of adductor muscle of Abalone, add 500 μL 1×TE lysate, cut it into pieces, add 50 μL 10% SDS, 7 μL proteinase K (20 mg / mL) successively, and lyse at 37°C for 30 minutes, then adjust to 55°C for lysing 1 -2h, until the lysate is clear; add 500 μL of phenol / chloroform / isoamyl alcohol s...

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Abstract

The invention discloses a SNP marking detection associated with growth trait of haliotis diversicolor and an application. The molecular marker is gene sequence shown as SEQ ID NO.1. The invention further discloses a primer AMY-FP and a primer AMY-EP for detecting the molecular marker; the primer AMY-FP is the sequence shown as SEQ ID NO.2, and the primer AMY-RP is the sequence shown as SEQ ID NO.3. The SNP site obtained through molecular marking, primer, and selection is applied to the selection and breeding of haliotis diversicolor. A parent is selected by using the primer and the method; the growth rate of the later breeding generations is obviously higher than the unselected control group; the molecular marker and the primer can efficiently, rapidly and simply be applied to the selection and breeding of haliotis diversicolor.

Description

technical field [0001] The invention relates to a SNP marker detection associated with the growth traits of abalone and its application, and belongs to the technical field of aquatic animal genetics and molecular marker-assisted breeding. Background technique [0002] Nine hole abalone ( Haliotis diversicolor supertexta ), also known as variegated abalone, belongs to the mollusk phylum, gastropod class, and abalone family. Abalone is known as the "Eight Treasures of Seafood" because of its well-developed abdomen and legs, delicious meat and high nutritional value. The research on the artificial breeding and breeding technology of Chinese abalone began in the 1970s. In the mid-1990s, due to the introduction of industrial abalone breeding technology, the artificial breeding of abalone became industrialized and gradually became an indispensable part of the aquaculture industry in southern my country. of cultivated species. However, with the rapid development of the abalone a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 刘东超刘建勇钱佳慧
Owner GUANGDONG OCEAN UNIVERSITY
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