SNP marking detection associated with growth trait of haliotis diversicolor and its application
A technology for growth traits and nine-hole abalone, which is applied in the fields of aquatic animal genetics and molecular marker-assisted breeding, can solve the problems of frequent occurrence of diseases, inhibiting the healthy development of the abalone industry, and economic loss of the nine-hole abalone breeding industry.
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[0034]Example: Correlation analysis of growth traits-related SNP sites 18C>G, 299C>T in the α-amylase gene of Abalone abalone, and its application in the breeding of high-yield Abalone abalone, according to the following steps :
[0035] (A) Genomic DNA extraction of Abalone;
[0036] (B) Screening of SNP sites of Abalone α-amylase gene;
[0037] (C) Locus 18C>G, 299C>T typing;
[0038] (D) Correlation analysis between 18C>G, 299C>T genotypes and growth traits;
[0039] (E) The method of site 18C>G, site 299C>T assisting in the selection of high-yielding Abalone abalone.
[0040] The specific operation is as follows:
[0041] (A) DNA extraction from Abalone abalone.
[0042] Take about 0.2 g of adductor muscle of Abalone, add 500 μL 1×TE lysate, cut it into pieces, add 50 μL 10% SDS, 7 μL proteinase K (20 mg / mL) successively, and lyse at 37°C for 30 minutes, then adjust to 55°C for lysing 1 -2h, until the lysate is clear; add 500 μL of phenol / chloroform / isoamyl alcohol s...
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