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MSTN (Myostatin) gene molecular marker selection method for growth characteristics of pelodiscus sinensis

A growth trait and molecular marker technology, applied in the field of molecular biology, to improve the efficiency of seed selection, and the selection method is simple and effective.

Active Publication Date: 2019-04-26
FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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Problems solved by technology

Facing the strong demand for Chinese soft-shelled turtle varieties, traditional breeding methods are far from being able to meet it. It is necessary to carry out molecular marker-assisted breeding of Chinese soft-shelled turtles to improve selection efficiency and speed up the breeding process.

Method used

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  • MSTN (Myostatin) gene molecular marker selection method for growth characteristics of pelodiscus sinensis
  • MSTN (Myostatin) gene molecular marker selection method for growth characteristics of pelodiscus sinensis
  • MSTN (Myostatin) gene molecular marker selection method for growth characteristics of pelodiscus sinensis

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Embodiment Construction

[0021] In order to better understand the present invention, the present invention will be further described below in conjunction with the examples, and the following examples are only to illustrate the present invention and not to limit it.

[0022] 1. PCR amplification and polymorphism detection of the MSTN gene DNA sequence of Chinese soft-shelled turtle

[0023] 1. PCR amplification of the DNA sequence of the soft-shelled turtle MSTN gene

[0024] According to the full-length sequence of the Chinese soft-shelled turtle MSTN gene (GenBank No: NW_005852453.1) downloaded by NCBI, the specific amplification primers P1 and P2 were designed, and 30 Chinese soft-shelled turtle genomic DNAs were used as templates, under the buffer environment of Taq PCR master mix, using Primers P1 and P2 were amplified under PCR conditions, and the product was purified and sequenced to obtain the target fragment with a length of 417bp.

[0025] The primer sequences are as follows:

[0026] P1: T...

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Abstract

The invention discloses an MSTN (Myostatin) gene molecular marker selection method for growth characteristics of pelodiscus sinensis. The MSTN gene molecular marker selection method comprises the following steps: taking an initiation codon of one SNP (Single Nucleotide Polymorphism) site on a first intron sequence in a pelodiscus sinensis MSTN gene as a starting point T2195C; screening a breedingmarker of an MSTN gene which is tightly correlated with the growth characteristics from a pelodiscus sinensis colony and screening and constructing a pelodiscus sinensis breeding colony with the breeding marker from a breeding colony of the pelodiscus sinensis. By adopting the method disclosed by the invention, the accuracy of selecting the growth characteristics of the pelodiscus sinensis can beimproved, a foundation is laid for developing molecular marker auxiliary breeding of the pelodiscus sinensis and the reference is provided.

Description

technical field [0001] The invention relates to a detection method and an application range of a SNP site of a Chinese soft-shelled turtle MSTN gene sequence, and belongs to the field of molecular biology. Background technique [0002] Growth traits are one of the important economic traits of soft-shelled turtles, which are affected by many genetic and environmental factors such as muscle growth and development. Selection of growth traits through phenotype often progresses slowly in genetics. Finding the main genes and genetic markers that control the growth traits of soft-shelled turtles, and using molecular marker-assisted selection can speed up the breeding process, which is of great significance for improving the growth performance of soft-shelled turtles. [0003] Myostatin gene (Myostation, MSTN) is a member of the growth transformation factor β (transforming growth factor-beta, TGF-β) superfamily, which negatively regulates the growth and development of animal muscle....

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806C12Q1/6888
CPCC12Q1/6806C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113
Inventor 黄龙何吉祥吴本丽陈静汪翔宋光同张烨
Owner FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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