RAPD marker tightly linking gene resistance to rice sheath blight

A technology for rice stripe and leaf blight resistance, applied in the field of molecular markers, can solve the problems of primary screening of unsuitable resistance, high technical threshold, poor stability and repeatability, etc., to improve breeding efficiency, simplify selection methods, and speed up Effects of the breeding process

Inactive Publication Date: 2010-06-02
JIANGSU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, researchers of rice stripe blight mostly draw on the indoor identification method established in Japan in the 1960s, but this method has high technical threshold, time-consuming and labor-intensive characteristics, making it unsuitable for the initial screening of resistance of a large number of materials in disease-resistant breeding
On the other hand, field resistance identification of rice stripe blight often shows poor stability and repeatability.
The technical bottleneck of the resistance identification method is also an important reason for the slow progress of rice stripe disease resistance breeding

Method used

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  • RAPD marker tightly linking gene resistance to rice sheath blight
  • RAPD marker tightly linking gene resistance to rice sheath blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Acquisition of Molecular Markers Linked to Rice Stripe Blight Resistance Gene

[0021] 1. Test materials

[0022] Plant materials: The susceptible variety Wuyujing 3 was used as the female parent and the resistant variety Zhendao 88 was used as the male parent to construct F 2 targeting groups. In the main season of 2006, F 2 The 200 families of the group were planted in the experimental field of Jiangsu Academy of Agricultural Sciences, and planted as a single plant. At the early stage of tillering, small tillers were picked out and transplanted to the test field for resistance identification. At the booting stage, some leaves of each individual plant were taken for SSR analysis. When ripe, harvest F 2:3 The seeds of the family were stored in the sun and used for the identification of seedling resistance in 2007.

[0023] Mediator SBPH: through screening and multiplication to reach the required amount of insects required by the experiment. The dot imm...

Embodiment 2

[0065] Example 2. Application of Molecular Markers Linked to Rice Stripe Blight Resistance Gene in Offspring of Hybrid Combinations with Zhendao 88 as Parent

[0066] Xudao 3, Xudao 4 and Lianjing 4 are three disease-resistant varieties obtained by crossing Zhendao 88 as the parent. Xudao 3 uses Zhendao 88 as the female parent and Taiwan rice C as the father. The offspring of this hybrid combination were screened. Xudao 4 was obtained from the offspring of the hybrid combination with Zhendao 88 as the female parent and Xu 41293 as the male parent, while Lianjing 4 was obtained from the hybrid combination with Zhendao 88 as the female parent and Zhongjing 3114 as the female parent. Obtained by screening the progeny of the crossing combination of the male parent. The resistance of Xudao 3, Xudao 4 and Lianjing 4 to rice stripe blight was predicted by RAPD marker OPO11 analysis (RAPD analysis method is the same as example 1, see figure 2 ), and the results of the resistance ide...

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Abstract

The invention discloses a molecular mark which is closely linked with the resistance gene of the rice stripe disease. The invention takes the F<2> population of the wuyujing No.3 / zhendao 88 as the mapping population according to the linkage and segregation principles in nature and obtains the RAPD mark of OPO11 which is closely linked with the resistance gene of the rice stripe disease according to the identification result of the disease resistance and the linkage analysis of the RAPD mark data. By applying the invention to the auxiliary selection of breeding of the species which is resistantto the rice stripe disease, the defects that the stability and repeatability of the identification of the rice stripe disease with the conventional breeding method is bad and the method costs time and labor and the technology requirement is high are solved; the result can simply the selection method and promote the breeding efficiency to accelerate the process of disease resistant species.

Description

technical field [0001] The invention relates to a molecular marker linked with rice stripe leaf blight resistance gene, which can be applied to molecular marker-assisted breeding and belongs to the fields of rice disease-resistant breeding and molecular biology. Background technique [0002] Rice stripe blight caused by rice stripe virus (RSV) was first discovered in Kanto, Japan in 1897, and later occurred in Korea, Ukraine and China. Since the virus was discovered in Jiangsu and Zhejiang in 1962 in my country, it has occurred in 16 provinces, municipalities, and autonomous regions across the country. At present, the rice fields in the lower reaches of the Yangtze River, mainly in Jiangsu Province, are still in the epidemic period. According to incomplete statistics, the occurrence area of ​​rice stripe blight in Jiangsu Province reached 27 million mu in 2004 alone, accounting for 79% of the rice area in Jiangsu Province. Experts predict that rice stripe blight will still s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 周彤王磊程兆榜季英华周益军
Owner JIANGSU ACAD OF AGRI SCI
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