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Molecular markers co-isolated with wheat low polyphenol oxidase activity gene qppo-5d

A technology for polyphenol oxidase and wheat, which is applied in the field of agricultural biology, can solve the problem that there is no low PPO active site, etc., and achieves the effects of improving breeding efficiency, simplifying selection methods, and speeding up the breeding process.

Active Publication Date: 2022-08-02
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report of a low PPO active site on the long arm of wheat 5D chromosome, and the development of a new molecular marker closely linked to this site will lay the foundation for the isolation and effective utilization of this gene

Method used

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  • Molecular markers co-isolated with wheat low polyphenol oxidase activity gene qppo-5d
  • Molecular markers co-isolated with wheat low polyphenol oxidase activity gene qppo-5d
  • Molecular markers co-isolated with wheat low polyphenol oxidase activity gene qppo-5d

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Acquisition of molecular markers linked to IDO580-21 wheat low PPO activity gene

[0035] 1. Plant material

[0036] Yangmai 158 (Su Zhong Shen Zi No. 179) was used as a control with high PPO activity. IDO580-21('Cadoux'(PI591905) / / 'Maya74' / M2) is preserved and provided by the Germplasm Resources Evaluation and Innovation Laboratory, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, and its grain PPO activity is extremely low . IDO580-21 and Yangmai 158 were crossed to construct a recombinant inbred line population.

[0037] 2. Evaluation method of grain PPO activity

[0038] In this study, dopa (L-DOPA / MOPS) was used as a substrate, and PPO catalyzed L-DOPA to form quinones, which in turn caused browning. Plump, non-damaged, and disease-free grains were selected for each material, weighed and placed in 96-well ELISA plates, with 1 grain per well, and 8 replicates and a blank control were set for each material. ...

Embodiment 2

[0054] Example 2. Application of molecular marker AX-110939270 in varieties (lines) in the middle and lower reaches of the Yangtze River

[0055] In order to verify the allele distribution of the AX-110939270 marker in varieties (lines) in the middle and lower reaches of the Yangtze River, and whether it can be applied to the breeding and improvement of varieties (lines) in the middle and lower reaches of the Yangtze River, this study used the AX-110939270 marker to quantify 53 middle and lower reaches of the Yangtze River. The varieties (lines) were screened, and the fluorescence scanning results were obtained by reading the fluorescence signals of KASP-labeled amplification products. The scanning data were analyzed by Kluster Caller software, indicating that these varieties contained heterozygous and homozygous high and low PPO activity genotypes. The cultivar in the X-axis region of the scatterplot is the "FAM" genotype, the cultivar in the Y-axis region is the "HEX" genotyp...

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Abstract

The invention discloses a molecular marker co-separated with a wheat low polyphenol oxidase activity gene QPpo-5D, which belongs to the category of agricultural biotechnology. A recombinant inbred line RIL population was constructed by crossing a wheat germplasm with a very low polyphenol oxidase activity and a wheat germplasm with high PPO activity, and the resistance gene was located in the staining 5DL5-0.76-1.00 segment by wheat molecular marker technology middle. In order to discover the new molecular marker of the low PPO activity gene QPpo-5D, a KASP marker AX-110939270 was developed using the SNP marker conversion method. As a new molecular marker of the low PPO activity gene QPpo-5D, this marker can be better applied to the assisted selection of wheat varieties with low PPO activity, thereby overcoming the shortcomings of conventional breeding, simplifying the selection method and improving the breeding efficiency, thereby accelerating the high white The breeding process of high-quality, high-quality varieties.

Description

technical field [0001] The invention relates to a molecular marker closely linked with a low polyphenol oxidase activity gene QPpo-5D of wheat, which can be applied to molecular marker-assisted breeding and belongs to the field of agricultural biotechnology. Background technique [0002] my country is one of the largest wheat producing and consuming countries in the world, and pasta is an important food for Chinese residents. Color browning is very common during flour storage and pasta processing. Browning not only reduces the whiteness of pasta, but also affects its nutritional value. Grain polyphenol oxidase (PPO) activity is the main reason for browning of flour products. Under the action of PPO, phenolic substrates react with oxygen to produce quinones, which can self-polymerize in plants or react with amino acids and proteins in cells, resulting in black or brown deposits, resulting in brown dough Change. PPO activity in wheat kernels can explain 50%-70% of dough co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCC12Q1/6895A01H1/04C12Q2600/13C12Q2600/156
Inventor 蔡瑾吴纪中付必胜刘彩云张巧凤郭炜吴小有
Owner JIANGSU ACAD OF AGRI SCI
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