93 results about "Recombinant inbred strain" patented technology

The molecular label closely linked with the active QTL of wheat fusarium blight resistance is prepared through hybridizing the disease-resistant wheat with the disease-sensitive wheat to obtain recombinant selfing line, and performing QTL and genetic linkage analysis to obtain the said molecular labels Xgwin 161, BARC 147 and Xgwin 493. They can be used to predict the level of the resistance to fusarium blight by detecting whether the DNA of wheat contains the said molecular labels or not.

The invention relates to a molecular marker tightly linked with a main effective genetic locus embodying sesame dampness resistance and application thereof. The main effective genetic locus qWHCHL09 is located on the 9th linkage group, and the molecular marker tightly linked with the main effective genetic locus is ZM428 whose sequences are as follows: ZM428F: AGGATGATGATGTGATGAGAG (5'-3') and ZM428R: CTGCTACTCCTTTTGTCTCTG (5'-3'). The molecular marker is obtained by hybridizing sesame 13 in sesame dampness-resistant varieties and a sensitive germplasm Yiyangbai to obtain F6-generation segregation population, namely a recombinant inbred line (RIL) population, and performing molecular genetic linkage analysis on the F6-generation segregation population. The molecular marker tightly linked with the main effective genetic locus embodying the sesame dampness resistance is applied to breeding of the sesame dampness-resistant varieties and screening of dampness-resistant traits of the sesame germplasm as well as early prediction; the auxiliary dampness resistance choosing target is clear; and the cost is relatively low.

The present invention relates to a molecular marker method closely linked with major QTL for powdery mildew of cucumber resistance, belonging to the field of biological technology. The invention comprises the following steps: hybridizing susceptible variety S94 of powdery mildew of cucumber with susceptible variety S06 of powdery mildew of cucumber, getting hybrid F1; acquiring F7 generation recombinant inbred lines by filial generation single seed descent method; separating DNA of individual cucumber leaves and performing PCR amplification; analyzing data and constructing cucumber genetic linkage map for molecular marker; applying to statistical analysis and QTL location; and determining molecular markers F, CSEPGN11, e23m18f and ME11EM9c linked with major QTL for resistance source S06 powdery mildew. The coincidence rate between the identification result of the invention and the field resistance has improved from 94 percent to 100 percent. By the molecular markers linked with major QTL for resistance source S06 powdery mildew, progeny and derived varieties thereof for the specific resistant source can be selected in early generation seedling without the influence of environment condition, saving cost and improving breeding and selection efficiency.

The invention provides molecular markers in close linkage with a large grain gene GS2 of rice and an application of the molecular markers. Through a recombinant inbred line (RIL) advanced grain shape segregation population, the grain shape major gene GS2 is finely located on a second chromosome and within a range of 33.2kb from GS2-6 to GS2-8, so as to obtain a series of molecular markers in close linkage with GS2. The molecular markers include a common data base marker RM3212 and 22 self-developed InDel markers (from GS2-1 to GS2-22). The molecular markers can be used for effectively detecting the GS2 genetic locus and further improving the breeding efficiency of large grain varieties (or parents).

The present invention relates to a molecular marker method linked with major QTL for cucumber downy mildew resistance and an application method, belonging to the field of biological technology. The molecular marker method: getting F2 filial generation by self-pollination of hybrid F1; acquiring F7 generation recombinant inbred lines by filial generation single seed descent method; describing the gene type of each strain of the recombinant inbred lines; constructing genetic linkage map for molecular marker; testing the downy mildew resistance of each strain of the recombinant inbred lines; and composite interval mapping for interval possible located in QTL. The application method: hybridizing S94 and derive verities thereof with other cucumbers and multiplying to F2 generation; separating genome DNA of single cucumber plant, and testing whether each cucumber plant has molecular marker method linked with major QTL for cucumber downy mildew resistance. The invention develops an efficient, rapid breeding technology, selects breeding variety with conventional downy mildew, reduces workload of breeder, and improves precise of selection and breeding efficiency.

The invention discloses a molecular marker Hf1-Indel related to hardness of watermelon flesh and application of the molecular marker Hf1-Indel. On the basis of a 97103 whole genome sequence and PI296341-FR resequencing information, the molecular marker Hf1-Indel related to hardness of the watermelon flesh is a closely interlocked molecular marker Hf1-Indel which is developed by designing a marker according to sites with alignment gaps or missing, and finely positioning hard flesh genes of watermelons by recombinant inbred line offspring target groups after hybridization of hard-flesh watermelons PI296341-FR and cultivated watermelon 97103. Experiments prove that the molecular marker Hf1-Indel can be used for preliminarily screening watermelon flesh hardness varieties, and the purpose of molecular assisted selection is achieved.

The invention relates to primers and a kit for detecting a molecular marker linked to major QTL for controlling corn stalk strength, a detection method for detecting corn stalk strength, and applications of the primers, the kit and the detection method, and belongs to the technical field of corn genetic breeding and molecular biology. According to the present invention, a recombinant selfing linepopulation containing 241 families is constructed through Zheng 58*D863F, and genetic linkage map analysis is performed, such that four QTL for controlling corn stalk strength are detected, wherein the high stalk strength major QTL qRPR1.07 is identified simultaneously at the position 1.07 of the chromosome 1 of the corn from Hainan Ledong and Henan Yuanyang Demonstration Base, and is positioned between two SSR markers bnlg1556 and umc2232 so as to explain nearly 20% of genetic variation; and the molecular marker linked to the major QTL for controlling corn stalk strength can directionally andprecisely screen corn materials with high stalk strength at the early stage of growth so as to save the breeding cost and the breeding time, and is used for lodging-resistant breeding of corn.

The invention relates to a rice variety IR36 black-streaked dwarf virus resistance site qRBSDV-1 and a molecular marking method and application thereof. According to the invention, a recombinant inbred line population constructed by crossing a rice black-streaked dwarf virus resistance variety IR36 and a black-streaked dwarf virus susceptible variety L5494 is taken as a positioning population, andthe black-streaked dwarf virus resistance identification is carried out by a field natural identification method, a resistance gene site, named qRBSDV-1, from a black-streaked dwarf virus resistancevariety IR36 is obtained, located between the markers AP-39.6 and RM104. The method verifies qRBSDV-1 by performing molecular detection and identification of the incidence of black-streaked dwarf virus by using a substitution line (No. N9) containing 93-11 introduced fragments only in the chromosome segment where qRBSDV-1 is located and parents in a Japanese sunny background. According to the invention, the resistance of the plant to the rice black-streaked dwarf virus can be predicted by detecting the bands at the molecular markers of AP-39.6 and RM104, and the breeding efficiency of the ricevariety resistant to the black-streaked dwarf virus is improved.

The invention discloses a protein, a gene and a function fragment involved in palmitic acid synthesis and application thereof. The invention provides a protein composed of amino acid sequences shown by a sequence 6, a sequence 4 or a sequence 2 in a sequence table, a DNA (Deoxyribonucleic Acid) molecule shown by a sequence 5, a sequence 3 or a sequence 1 in the sequence table, and a DNA fragment shown by a sequence 7. In the invention, a recombinant inbred strain colony is constructed by utilizing a common material B73 and a high oil system By 804, a major QTL-Pa 19 for controlling corn oil palmitic acid is positioned on a ninth chromosome 9.02 Bin, and the LOD (Level of Detail) of the major QTL-Pa can reach 27, and can be used for interpreting 42% of genetic variation. By means of the method of based cloning, the invention accurately positions the major QTL-Pa for controlling the palmitic acid content of corn kernels and the proportion of kernel saturated/unsaturated fatty acid in Beijing high oil, clones the function gene of QTL-Pa 19, probes the functional sites thereof, develops the functional markers, and has great value for improving the quality of corn kernels.

The invention relates to a watermelon flesh hardness-related molecular marker Hf2-Indel and application thereof. A marker is designed on the basis of a whole-genome sequence of 97103 and re-sequencing information of PI296341-FR according to an insertion/deletion locus, and the closely linked molecular marker Hf2-Indel is developed by accurately mapping hard flesh genes by virtue of a recombinant inbred line progeny mapping population obtained after hybridization of wild hard-flesh watermelons PI296341-FR and cultivated watermelons 97103. Experiments show that the molecular marker Hf2-Indel can be used for preliminarily screening watermelon flesh hardness varieties to fulfill the aim of molecular assisted selection.

The invention discloses a molecular marker of rice long and slender grain gene as well as application thereof. The molecular marker of the rice long and slender grain gene is prepared by the followingsteps: carrying out hybridization by taking a long and slender grain variety, namely Tai-feng B, as a female parent and a short grain variety, namely Te-san-ai No. 2, as a male parent so as to obtainF1; allowing bagged self-pollination of the F1 so as to obtain F2 seeds; planting F2 population, and adopting a single seed descend method so as to obtain recombinant inbred populations composed of 170 families; performing investigation and statistics on grain lengths, grain widths and length-width ratio phenotypes of segregated populations so as to select characteristic plants containing SG7 from the recombinant inbred populations; and then, developing a molecular marker SG7-18 on basis of SG7 candidate genes according to sequence differences of 18 bp insertion/deletion of LOC_Os07g41200 gene between the long and slender grain variety Tai-feng B and the short grain variety Te-san-ai No. 2, thereby obtaining the molecular marker SG7-18 of the rice long and slender grain gene. The molecular marker of the rice long and slender grain gene is used for detecting SG7 genotype in rice varieties; and the rice long and slender grain gene is further used for marking and cultivating long-and-slender-grain-type rice varieties.

The invention belongs to the field of molecular breeding of crops, and relates to an identifying and utilizing method of a rice wide-compatibility recessive male nuclear sterile line. Nonglutinous rice 'Teqing' and the wide-compatibility variety '02428' are adopted for constructing a recombinant inbred line (RIL), a selfing line with the fertility feature is selected, a novel sterile line RI127S is cultivated and screened, and the sterile line can be used as an intermediate material for rice rotational breeding. The identifying and utilizing method has the advantages that (1) the sterility of the male sterile line is controlled by recessive genes, and is not influenced by length of illumination and temperature under the normal growth condition, and the male sterile line is an ideal recurrent selection intermediate material for rice; (2) the sterile line RI127S contains wide-compatibility genes, the genome of the sterile line RI127S is a mixture of nonglutinous rice and japonica rice varieties, after hybridization with nonglutinous rice and japonica rice, the hybridization seeds are fertile, the F1 fruitage rate of nonglutinous rice is 85%, and the F1 fruitage rate of japonica rice is 80%; (3) the inbred line RI127 has the high yield property intrinsically, and can be taken as a parent of the good stock, and when the RI127 is taken as the sterile line, the process of artificial emasculation for hybridization can be saved.

The invention discloses a method for constructing a QTL positioned new-group-linkage F2 group.The method comprises the steps of selecting three selfing lines which are from different sources and have great genetic background differences, utilizing the three selfing lines for hybridization to obtain three hybrids, adopting a single-grain genetic method for performing selfing on the three hybrids for seven generations or more till all the lines in the group are purified, and selecting and breeding three recombination selfing lines; constructing a linkage F2 group, performing recombination on every two selfing lines, and performing hybridization on every two extracted lines randomly to obtain n combinations; finally, obtaining three F2 groups, and forming a linkage F2 group, wherein the number of the hybridization combinations of the obtained linkage F2 group is 3n.According to the constructed linkage F2 group, QTL positioning is researched, the advantages of a temporary group and the advantages of a permanent group are synthesized, meanwhile, the defect existing in QTL positioning of the permanent F2 group is overcome, the accuracy of QTL positioning tests is improved, and the group is novel and can precisely position QTL and promote forward development of hybrid advantage theory research.

The invention relates to a molecular marker closely linked with bacterial wilt resistance of cultivated peanuts and application of the molecular marker. A recombinant inbred line group taking far-hybrid 9102 and wt09-0023 as parents is utilized to construct a high-density linkage map, and a resistance major QTL (qBWR-A12) which can be repeated in a plurality of growth periods and for many years is positioned. KASP primers are developed according to SNP information of two wings of the major QTL, an SNP molecular marker capable of being applied to far-hybrid 9102 blood-related materials and non-blood-related materials is obtained, the SNP marker is located at the 4097252bp position of the 12th chromosome, and linkage between the marker developed based on the QTL and bacterial wilt resistance is tighter. According to the molecular marker, the step of bacterial wilt inoculation identification or disease nursery identification is omitted, the bacterial wilt resistance information of the material can be rapidly and accurately obtained only by detecting the SNP genotype, and the molecular marker can be applied to molecular breeding of peanut bacterial wilt resistance.

A technical method of creating a parent of polygene type colony heterosis utilization is a new technology of breeding the parent of the polygene type colony heterosis utilization. The technical method of creating the parent of the polygene type colony heterosis utilization can effectively polymerize merits of resource materials. The technical method of creating the parent of the polygene type colony heterosis utilization includes the following steps of constructing a recombinant inbred line through two subject parents, wherein the two parents hybridization F1 is obvious in superiority and preferably one of the two parents is a local main cultivar, selecting a monophyly from an F5 generation, wherein agriculture characters of the monophyly is basically consistent with that of a female parent, screening a monophyly from an F6 generation through a markers assisted selection technology, wherein genetic background of the monophyly is approximate to a male parent, paring the monophyly screened from the F6 generation and the original female parent, identifying characters including heterosis, resistance, uniformity, quality and the like of an F2 genration, and breeding a new material, wherein the new material and the female parent have complementary advantages, the plant type of the new material is ideal, the resistance of the new material is wide, the quality of the new material is good, characters influencing the colony regularity are all shown consistently, the genetic background difference of the new material is large, and heterosis of the F2 generation is prominent in superiority. The technical method of creating the parent of the polygene type colony heterosis utilization effectively solves the problem that parent selection of the polygene type colony heterosis utilization is difficult and enables teleonomy of the parent breeding to be more explicit and controllability of the parent breeding to be stronger.

The invention relates to the field of cotton variety breeding, in particular to a crossbreeding method for pyramiding cotton superior traits. The method includes steps: taking a cotton variety authorized in Shandong Province which is a main cotton production region in the Yellow River basin as a female parent, taking two lines with fiber superior traits as male parents, and performing crossbreeding of the female parent with the male parents respectively to obtain a generation F1, wherein at least one of the male parents belongs to chromosome segment substitution lines from gossypium hirsutum and gossypium barbadense or recombinant inbred lines thereof; collecting pollens of stamens of generation F1 plants, mixing, performing pollination backcrossing on the generation F1 plants, and harvesting to obtain a double-cross generation F2; subjecting the generation F2 to continuous selfing for 3-10 times, and screening to obtain cotton superior traits pyramided line. By means of specific multi-parent double-cross pyramiding breeding, the character of high yield of the female parent is kept in the final generation, combining abilities of varieties are explored, pyramiding of superior traitsand certain lines is realized, a superior variety improving term is shortened, and genetic improvement efficiency is improved.

The invention discloses a method for culturing a variety (line) with high phosphorus efficiency by using a soybean root configuration near-isogenic line, and belongs to the field of genetic improvement and utilization of phosphorus nutrition of crops. The method comprises main steps of building recombination inbred lines (RILs) of traits of phosphorus efficiency related root systems, building near-isogenic lines (NILs) of efficient root configurations, hybridizing, transferring and screening high-phosphorus-efficiency quantitative trait locuses (QTLs), and the like. The method disclosed by theinvention does not need to depend on a gene cloning and transgenosis technology with high time consumption and high cost, avoids phenomena of difficulty in phenotypic selection, linkage drag and thelike which are caused by conventional hybridization and breeding, realizes fast integration of efficient, high-yield and high-quality genes of the phosphorus nutrition, can obtain a soybean variety (line) with good root configurations and high phosphorus efficiency efficiently and accurately, and greatly improves the breeding efficiency of the variety with high phosphorus efficiency.

The invention discloses a method for quickly selecting paddy rice with sheath blight resistance and qSBRLEYD2A genes. The method includes carrying out PCR (polymerase chain reaction) amplification ondifferent strains in segregation population of recombinant inbred lines by the aid of D223 labels; selecting strains with banding patterns consistent with No.4 Yangdao which is a parent. Lemont and the No.4 Yangdao are assembled to obtain the segregation population of the recombinant inbred lines. The selected strains are strains with qSBRLEYD2A alleles with sheath blight resistance. Compared withthe traditional field inoculation identification methods, the method has the advantages that the method is simple, convenient and speedy, and stable paddy rice materials with sheath blight resistancecan be selected without inoculation identification; the method is high in selection accuracy as compared with field inoculation methods, and the breeding efficiency can be greatly improved.

The invention discloses a molecular marker separated from a low-polyphenol-oxidase-activity gene QPpo-5D of wheat and belongs to the technical field of agrobiology. A recombinant inbred line RIL colony is constructed through hybridizing wheat germ plasm with extremely low polyphenol oxidase activity and wheat germ plasm Yangmai with high PPO activity, and a resistant gene is located to a stained 5DL5-0.76-1.00 section through a wheat molecular marker technology. In order to explore novel molecular markers of the low-PPO-activity gene QPpo-5D, a KASP marker AX-110939270 is developed by using anSNP marker conversion method. The marker, as a novel molecular marker of the low-PPO-activity gene QPpo-5D, can be better applied to assisted selecting of low-PPO-activity varieties of the wheat, sothat shortages of conventional breeding are overcome, a selecting method is simplified, the efficiency of breeding is increased, and thus, a breeding progress of high-whiteness and high-quality varieties is accelerated.

The invention discloses a molecular marker linked with a rice cold-tolerant gene qSF12 and application of the molecular marker in identification of rice cold tolerance. According to the invention, a Jileng-1/Miyang 23 recombinant inbred line group (RIL) is utilized, the invention successfully positions a gene locus qSF12 related to the cold tolerance of rice in the booting stage on the twelfth chromosome of the rice, a cold-tolerant favorable allele of the qSF12 comes from a cold-tolerant parent Jileng-1 and is linked with a molecular marker STS12b, a method for identifying or assisting in identifying the cold resistance of the rice in the booting stage is provided based on the molecular marker STS12b, and the method can be used for identifying and molecular breeding of cold-resistant ricevarieties in the booting stage.