10 results about "Recombinant inbred strain" patented technology

The present disclosure relates to the field of agricultural technology, and particularly relates to a crop phenotype prediction method and a crop phenotype prediction device. The crop phenotype prediction method comprises: determining a genotype of a parent; determining phenotype data of the parent under a current environment; inputting the genotype of the parent and the phenotype data of the parent into a trained prediction model to determine a predicted value of a phenotype of a crop under the current environment, wherein the prediction model is trained according to genotypes of multiple strains of a recombinant inbred line of the parent and phenotype data of the multiple strains of the recombinant inbred line in multiple environments. According to the present disclosure, the predicted value of the phenotype of the crop under the environment can be directly determined according to the genotype of the parent and the phenotype characteristics of the parent under any environment, the influence of the genotype of the parent and environmental factors on the phenotype of the crop is comprehensively considered through the prediction model, the phenotype of the crop is more quickly and accurately predicted, the period and cost of breeding are effectively shortened, and the prediction has high accuracy and reliability.

The application discloses a SNP site related to soybean protein content and application thereof. The SNP site related to soybean protein content disclosed by the application is located at 35708082 of chromosome 19 of a soybean genome, and the polymorphism is C or A. The application also discloses a HRM melting curve method for identifying soybean protein content developed based on the SNP site, and the method is verified in a four-way recombinant inbred line material. The verification result shows that the SNP site discovered by the application is significantly related to the soybean seed protein content. The application provides a theoretical basis for molecular assisted breeding of the soybean protein content, and has important significance for cultivating new soybean varieties with high protein content.

This invention discloses a gene controlling spike length and grain number per spike in wheat. This gene is located on chromosome 2B using a recombinant inbred line population and high-density SNP markers, within the 657.82–664.40 Mb region of chromosome 2B in the wheat Chinese spring reference genome. A candidate gene, named TaeEF1A, was identified based on the wheat reference genome sequence, RNA-seq, and gene function. Gene editing of TaeEF1A further validated its function in regulating spike length, spikelet number, and grain number per spike. Through gene sequencing and analysis of the gene sequence in a large number of germplasm resources, key variations affecting spike length and grain number per spike were identified in the 3'-UTR of TaeEF1A. Based on this, a detection marker was designed for this gene, which can be used to detect superior allelic variants of the TaeEF1A gene. This marker can be easily, rapidly, and with high throughput applied to marker-assisted breeding to increase spike length, spikelet number, and grain number per spike, thereby increasing yield.

The invention discloses a KASP molecular marker linked with a stripe rust resistance gene QYr.CM62-2BL of Chuan wheat 62 and application of the KASP molecular marker. The KASP molecular marker comprises KASP-AX-109507945 and KASP-AX-109844182, wherein the nucleotide sequences of the KASP-AX-109507945 and the KASP-AX-109844182 are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. The invention further discloses a preparation method of the KASP molecular marker. According to the invention, a recombinant inbred line group constructed by Chuanmai 62 and a susceptible variety Avock S is combined with SNP (Single Nucleotide Polymorphism) chip genotyping and multi-environment adult-plant-stage stripe rust resistance identification, QTL (Quantitative Trait Loci) positioning analysis is carried out, and a stable major stripe rust resistance new locus QYr.CM62-2BL in multiple environments is identified. According to the present invention, the primer is developed by using the QYr.CM62-2BL close linkage KASP molecular marker, and the rapid and accurate genotype identification is performed on the disease-resistant site;

The invention discloses a dCAPS molecular marker related to soybean glycine content and application of the dCAPS molecular marker, and belongs to the technical field of molecular marker breeding. According to the invention, a recombinant inbred line constructed by a high-protein variety Nannong 26 and a low-protein germplasm Williams82 is used as a material for genetic analysis and QTL (quantitative trait loci) positioning, finally, an SNP (single nucleotide polymorphism) site related to the glycine content is determined, the SNP site is located at the 8589041bp position of a No.8 chromosome and is named as qGly8-T439A SNP site, and the polymorphism is A / T. Further, a dCAPS molecular marker primer pair is developed and is used for identifying or assisting in identifying the glycine content of the soybean. Experiments show that the glycine content of the soybean with the genotype of TT at the qGly8-T439A SNP site is obviously higher than that of the soybean with the genotype of CC. The dCAPS molecular marker provided by the invention can realize efficient and rapid screening of soybean varieties with high glycine content, and significantly accelerates the breeding process of new high-quality soybean varieties.

The invention belongs to the field of molecular biology, and provides a functional molecular marker of a major gene AhNAC2 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker. The invention discloses a major site qSOCA08-1 for regulating and controlling the oil content of peanut seed kernels, and the phenotypic variation of 11.41-20.97% is explained. The qSOCA08-1 is finely positioned in a 352.2 kb physical interval between a No.8 chromosome marker Tif2. A08.38582892 and a No.8 chromosome marker Tif2. A08.38935104, and a candidate gene of the qSOCA08-1 is confirmed to be AhNAC2. The invention also discloses a method for identifying the qSOCA08-1. The 285th basic group of the coding sequence of the AhNAC2 shows C-A nonsense mutation between parents Jihuansweet No.1 and W191, and the early termination can cause the function loss of the AhNAC2. A competitive allele specific PCR molecular marker is developed on the basis of a C-A mutation SNP site, the association between the molecular marker and the oil content of the seed kernel is successfully verified in a peanut germplasm resource material and a Yuhua 15 * ST001 recombinant inbred line population, and the important value of the marker in marker-assisted selection breeding of the high oil content of the seed kernel of the peanut is verified.

The invention belongs to the field of molecular biology, provides a functional molecular marker of a major gene AhARF2 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker, discloses a major site qSOCA08-3 for regulating and controlling the oil content of the peanut seed kernels, and explains phenotypic variation of 14.91%-17.31%. The qSOCA08-3 is finely positioned to a physical interval of 349.6 kb between the Tif2. A08.49681404 and the Tif2. A08.50031016, and a candidate gene of the qSOCA08-3 is confirmed to be AhARF2. The qSOCA08-3 has the advantages that the qSOCA08-3 can be used for identifying the The AhARF2 coding sequence shows 275bp deletion between parents Jianhua No.1 and W191 and is accompanied by 7bp replacement, so that the code shift mutation of the coding sequence is caused, and the protein translation is terminated in advance. According to the invention, the KASP molecular marker is developed on the basis of a mutant sequence, the association between the KASP molecular marker and the oil content of the seed kernel is successfully verified in a Yuhua 15 * ST001 recombinant inbred line population, and the important value of the marker in the marker-assisted selection breeding of the high oil content of the seed kernel of the peanut is verified.

The invention relates to the technical field of molecular biology and plant genetic breeding, in particular to application of a QTL (quantitative trait loci) qCsr12 to identification of chilo suppressalis resistance traits of rice. According to the invention, an advanced-generation recombinant inbred line genotype constructed by hybridizing a rice anti-rice-borer variety WFS and an insect-susceptible variety 923 is combined with the insect weight of stem inoculation to carry out genetic linkage analysis, and the anti-rice-borer QTL locus qCsr12 of the WFS in a chromosome 12 is identified and is located between indel1462 and indel1522. The specific molecular marker can be used for effectively detecting the major anti-borer QTL locus qCsr12 in an anti-borer variety WFS and derived varieties thereof, so that the selection efficiency of anti-borer rice is greatly improved, and a rice line containing the major anti-borer locus of the WFS is obtained.

The invention belongs to the field of molecular biology, and provides a functional molecular marker of a major gene AhWRI1 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker. The molecular marker is located at InDel sites of 45bp, 716bp, 738bp-45bp, 718bp and 423bp of a No.8 chromosome of peanuts, the sequence of the molecular marker is SEQ ID NO.1 or SEQ ID NO.2, and a candidate gene of the molecular marker is named as AhWRI1. The KASP molecular marker is successfully developed on the basis of LTR reverse transcription transposon insertion / deletion polymorphism, the association between the KASP molecular marker and the oil content of the seed kernel is verified in a Yuhua 15 * Yueyan 20 recombinant inbred line population and a germplasm resource material, the accuracy of the marker is proved, and the KASP molecular marker can be applied to high-oil-content molecular marker-assisted selection breeding of the peanut seed kernel.