22 results about "Recombinant inbred strain" patented technology

The invention provides a method for constructing a rice epigenetic recombinant inbred line, and belongs to the technical field of molecular biology and genetic breeding. According to the method for constructing the rice epigenetic recombination inbred line, a fertile rice DDM1 gene function weakening mutant is obtained through a CRISPR / Cas9 gene editing technology and hybridized with wild type rice with the same background to obtain F1, then the F1 and the wild type rice are subjected to backcrossing to obtain BC1F1, single-plant offspring selfing with the DDM1 genotype being the wild type is selected, selfing is conducted for multiple generations through a single-grain transmission method, and the rice epigenetic recombination inbred line is obtained. According to the present invention, the strain population with stable inheritance is constructed, such that the rice epigenetic recombinant inbred line is obtained, and has characteristics of rich phenotype, genetic and epigenetic variation so as to provide important population resources for batch identification and the like of important agronomic shape functional sites of rice.

The invention discloses a molecular marker for identifying the protein content of soybean seeds and application of the molecular marker. The invention further discloses an SNP site related to the soybean seed protein content, the SNP site is located at the 16083582nd site of the No.4 chromosome of a soybean genome, and the polymorphism is G or A. The invention further develops a molecular marker for identifying the soybean seed protein content on the basis of the SNP site and verifies the molecular marker in a four-way recombinant inbred line material. A verification result shows that the found SNP site is remarkably related to the soybean seed protein content character, and the seed protein content of a Hap1 genotype soybean material with the SNP site being G is remarkably higher than that of a Hap2 genotype soybean material with the SNP site being A. The invention provides a theoretical basis for soybean molecule-assisted breeding, and has important significance for cultivating high-quality new varieties.

The present application relates to the field of crop genetic breeding and molecular biology, and particularly relates to a highland barley beta-glucan screening index system and a major gene positioning method, which comprises the following steps: firstly, a three-dimensional coupling screening system of genetic stability, functional activity and agronomic adaptability is constructed, the index combination weight is determined by using an entropy weight method and an analytic hierarchy process, and an AMMI model is combined to correct the environmental effect and calculate the comprehensive score of the germplasm; secondly, a recombinant inbred line and a natural germplasm double positioning population is constructed, and near-infrared spectroscopy is combined with a Transformer model to quickly obtain phenotype data; thirdly, a candidate major effective segment is obtained through double population joint positioning, a major effective gene is screened out by combining a transcriptome and a metabolome co-expression network, and a functional molecular marker is developed. The present application realizes accurate evaluation of highland barley germplasm and accurate positioning of major effective genes, and the phenotype detection is efficient and lossless, the molecular marker selection is accurate, and the present application can also be applied to other cereal crops.

The present disclosure relates to the field of agricultural technology, and particularly relates to a crop phenotype prediction method and a crop phenotype prediction device. The crop phenotype prediction method comprises: determining a genotype of a parent; determining phenotype data of the parent under a current environment; inputting the genotype of the parent and the phenotype data of the parent into a trained prediction model to determine a predicted value of a phenotype of a crop under the current environment, wherein the prediction model is trained according to genotypes of multiple strains of a recombinant inbred line of the parent and phenotype data of the multiple strains of the recombinant inbred line in multiple environments. According to the present disclosure, the predicted value of the phenotype of the crop under the environment can be directly determined according to the genotype of the parent and the phenotype characteristics of the parent under any environment, the influence of the genotype of the parent and environmental factors on the phenotype of the crop is comprehensively considered through the prediction model, the phenotype of the crop is more quickly and accurately predicted, the period and cost of breeding are effectively shortened, and the prediction has high accuracy and reliability.

The application discloses a SNP site related to soybean protein content and application thereof. The SNP site related to soybean protein content disclosed by the application is located at 35708082 of chromosome 19 of a soybean genome, and the polymorphism is C or A. The application also discloses a HRM melting curve method for identifying soybean protein content developed based on the SNP site, and the method is verified in a four-way recombinant inbred line material. The verification result shows that the SNP site discovered by the application is significantly related to the soybean seed protein content. The application provides a theoretical basis for molecular assisted breeding of the soybean protein content, and has important significance for cultivating new soybean varieties with high protein content.

The invention relates to a functional molecular marker for white seed coat characters of peanut powder and application, and belongs to the field of molecular biology. The method comprises the following steps: carrying out BSA (Bovine Serum Albumin) analysis on a recombinant inbred line group taking far hybrid 9102 and Tifrunner as parents by constructing a mixed pool, and positioning two major QTLs linked with the character of the pink white seed coat; two candidate genes AhbHLH-2A and AhbHLH-2B are screened by analyzing a family genotype recombination unit of an RIL group, and the genes are knocked out from peanuts, so that the functions of the genes are verified. According to the present invention, two groups of KASP primers (A02.97750456 and A12.113996432) are developed according to the difference of the genome sequence for regulating the white seed coat of the peanut powder, and the authenticity and the linkage of the InDel site of the A02.97750456 and the SNP site of the A12.113996432 are verified in the "far hybrid 9102 * Tifrunner" RIL population and the germplasm resource material, and the molecular marker can rapidly and accurately obtain the white seed coat character of the peanut powder, and can be used in the peanut molecule-assisted marker breeding.

This invention discloses a gene controlling spike length and grain number per spike in wheat. This gene is located on chromosome 2B using a recombinant inbred line population and high-density SNP markers, within the 657.82–664.40 Mb region of chromosome 2B in the wheat Chinese spring reference genome. A candidate gene, named TaeEF1A, was identified based on the wheat reference genome sequence, RNA-seq, and gene function. Gene editing of TaeEF1A further validated its function in regulating spike length, spikelet number, and grain number per spike. Through gene sequencing and analysis of the gene sequence in a large number of germplasm resources, key variations affecting spike length and grain number per spike were identified in the 3'-UTR of TaeEF1A. Based on this, a detection marker was designed for this gene, which can be used to detect superior allelic variants of the TaeEF1A gene. This marker can be easily, rapidly, and with high throughput applied to marker-assisted breeding to increase spike length, spikelet number, and grain number per spike, thereby increasing yield.

The invention discloses a KASP molecular marker linked with a stripe rust resistance gene QYr.CM62-2BL of Chuan wheat 62 and application of the KASP molecular marker. The KASP molecular marker comprises KASP-AX-109507945 and KASP-AX-109844182, wherein the nucleotide sequences of the KASP-AX-109507945 and the KASP-AX-109844182 are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. The invention further discloses a preparation method of the KASP molecular marker. According to the invention, a recombinant inbred line group constructed by Chuanmai 62 and a susceptible variety Avock S is combined with SNP (Single Nucleotide Polymorphism) chip genotyping and multi-environment adult-plant-stage stripe rust resistance identification, QTL (Quantitative Trait Loci) positioning analysis is carried out, and a stable major stripe rust resistance new locus QYr.CM62-2BL in multiple environments is identified. According to the present invention, the primer is developed by using the QYr.CM62-2BL close linkage KASP molecular marker, and the rapid and accurate genotype identification is performed on the disease-resistant site;

The invention belongs to the technical field of molecular biology and genetic breeding, and relates to a molecular marker closely linked with a major site of the internode length of a sesame capsule and application of the molecular marker. According to the invention, a major QTL site for controlling the internode length of a sesame capsule is positioned in the 10.41-10.73 Mb interval of the twelfth chromosome of the sesame by utilizing the genotype and phenotype of a recombinant inbred line, the explainable phenotypic variation rate reaches 27.39%, and the major QTL site is named as qCIL; the invention also develops a PAV molecular marker closely linked with qCIL, the PAV molecular marker is named as PAV1216, and the primer sequences of the PAV molecular marker are: PAV1216F: 5 '-TTTCCCGCCTAAAGCCATGT-3', and PAV1216R: 5 '-GTGGTTCCGGTGGTGTTTG-3'. The molecular marker PAV1216 and the primer sequence of the molecular marker PAV1216 are applied to auxiliary selection of sesame capsule internode length markers. The sesame can be subjected to genotype identification through the molecular marker, and the breeding process of sesame ideal plant type germplasm is accelerated by establishing a molecular marker-assisted selective breeding system of sesame capsule internode length; and meanwhile, the traditional phenotype is converted into genotype selection, so that the accuracy and scientificity of selection are improved.

The invention relates to the technical field of molecular biology and genetic breeding, in particular to a peanut pod cracking force site, a molecular marking method and application. The method comprises the following steps: S1, performing phenotype identification and QTL (Quantitative Trait Locus) analysis, and performing QTL scanning through ICIMapping V4.2 software in combination with an existing genetic map and population genotype information; s2, acquiring an SNP site and developing a marker, and discovering that A / G single nucleotide polymorphism variation exists at the 139316601 position of the B04 chromosome of the VX7G96 gene and causes termination mutation through analysis of gene annotation information in an interval and analysis of sequence difference between parents; and S3, verifying the molecular marker B04-139316601F and the molecular marker B04-139316601R which are linked with the pod splitting character, and carrying out qPD. B04 site detection on a strain of a recombinant inbred line group by utilizing the molecular marker B04-139316601F or the molecular marker B04-139316601R. The peanut pod cracking force site qPD.B04 and the molecular marking method provided by the invention have remarkable technical advantages. 10.17%-13.61% of the qPD.B04 locus showing variation can be explained through accurate positioning, and close association between key candidate genes and pod cracking characters is determined by combining functional analysis of the homologous gene VX7G96 of arabidopsis SEP2.

The invention relates to the technical field of soybean genetic breeding, in particular to positioning and application of a soybean fatty acid character SNP (Single Nucleotide Polymorphism) marker. Comprising the following steps: constructing a recombinant inbred line (RIL) group obtained by hybridizing a high-oleic-acid parent variety 'Glysoja-HO' and a low-oleic-acid parent variety 'Glysoja-LO'; performing whole genome re-sequencing on all individuals in the population to obtain SNP genotype data covering 20 chromosomes of the soybean; determining fatty acid composition phenotype data of individuals in the group in the three environments through a gas chromatographic method; performing whole genome association analysis on the SNP data and the phenotype data by adopting a linkage imbalance-linkage analysis integration model; screening an SNP (Single Nucleotide Polymorphism) marker% associated with the target fatty acid character; and determining a core function marker through haplotype block analysis and independent natural group verification. The invention provides a method for accurately positioning the soybean fatty acid character core SNP marker which is stable in environment, remarkable in effect and capable of being efficiently used for molecular breeding and variety identification.

The invention discloses a dCAPS molecular marker related to soybean glycine content and application of the dCAPS molecular marker, and belongs to the technical field of molecular marker breeding. According to the invention, a recombinant inbred line constructed by a high-protein variety Nannong 26 and a low-protein germplasm Williams82 is used as a material for genetic analysis and QTL (quantitative trait loci) positioning, finally, an SNP (single nucleotide polymorphism) site related to the glycine content is determined, the SNP site is located at the 8589041bp position of a No.8 chromosome and is named as qGly8-T439A SNP site, and the polymorphism is A / T. Further, a dCAPS molecular marker primer pair is developed and is used for identifying or assisting in identifying the glycine content of the soybean. Experiments show that the glycine content of the soybean with the genotype of TT at the qGly8-T439A SNP site is obviously higher than that of the soybean with the genotype of CC. The dCAPS molecular marker provided by the invention can realize efficient and rapid screening of soybean varieties with high glycine content, and significantly accelerates the breeding process of new high-quality soybean varieties.

The invention belongs to the field of molecular biology, and provides a functional molecular marker of a major gene AhNAC2 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker. The invention discloses a major site qSOCA08-1 for regulating and controlling the oil content of peanut seed kernels, and the phenotypic variation of 11.41-20.97% is explained. The qSOCA08-1 is finely positioned in a 352.2 kb physical interval between a No.8 chromosome marker Tif2. A08.38582892 and a No.8 chromosome marker Tif2. A08.38935104, and a candidate gene of the qSOCA08-1 is confirmed to be AhNAC2. The invention also discloses a method for identifying the qSOCA08-1. The 285th basic group of the coding sequence of the AhNAC2 shows C-A nonsense mutation between parents Jihuansweet No.1 and W191, and the early termination can cause the function loss of the AhNAC2. A competitive allele specific PCR molecular marker is developed on the basis of a C-A mutation SNP site, the association between the molecular marker and the oil content of the seed kernel is successfully verified in a peanut germplasm resource material and a Yuhua 15 * ST001 recombinant inbred line population, and the important value of the marker in marker-assisted selection breeding of the high oil content of the seed kernel of the peanut is verified.

The present application belongs to the technical field of molecular biology and genetic breeding, and relates to a molecular marker SNP6804 closely linked to a main-effect QTL site of golden yellow sesame seeds and application thereof; the present application locates a main-effect QTL site of controlling golden yellow sesame seeds on the 6th linkage group LG06 of sesame by using phenotypes of recombinant inbred lines and high-density genetic maps, can explain 29.46% to 35.79% of the phenotypic variation rate in different environments, and is named as qSC_LG06; a SNP molecular marker closely linked to qSC_LG06 is simultaneously developed, and is named as SNP6804, the primer sequence of which is: SNP6804F1: 5'-GACAAAGTTGCGATACGCCA-3', SNP6804F2: 5'-TCGACGACAAAGTTGCGATACGACG-3', SNP6804R: 5'-TGCAGGACTCAGTCTTCATGGGTG-3'. Through the application of the molecular marker of the present application, molecular marker assisted selection breeding of golden yellow sesame seeds can be carried out, the breeding cycle is shortened, the cumbersome procedure of identifying the color of each generation in the breeding process is saved, a large amount of labor is saved, the cost is reduced, the work efficiency is improved. Meanwhile, the traditional phenotype is converted into genotype selection, and the accuracy and scientificity of selection are improved.

The invention discloses an SSR molecular marker closely linked with a mung bean fomesafen-resistant herbicide gene and application of the SSR molecular marker, and belongs to the technical field of mung bean molecular breeding. According to the invention, a recombinant inbred line group formed by Liaoning LZ177 and Hubei LD235 is taken as a test material, a herbicide resistance major QTL site is obtained by utilizing SSR markers, and the SSR markers which are positioned in a No.11 chromosome and are closely linked with the major QTL site are SSR2755 and SSR2968. The molecular marker closely linked with the mung bean fomesafen-resistant herbicide gene disclosed by the invention can be used for well typing the mung bean fomesafen-resistant herbicide gene, and can be used for mung bean fomesafen-resistant molecular marker assisted breeding; molecular auxiliary technical support is provided for early identification and screening of herbicide resistance characters of mung beans, and the method has important theoretical and practical significance in accelerating genetic breeding and improvement processes of mung bean varieties.

The invention relates to application of a bPLA12 gene in influencing the content of palmitic acid (C16: 0) in rice. Effect verification is carried out on QTL qbPLA12 for controlling the content of C16: 0, which is initially positioned by a recombinant inbred line derived by hybridization of Hua 6S (Hua6S) and B805D-MR-16-8-3 (B805D), and the qbPLA12 is found to be capable of regulating the content of C16: 0 in rice, and the gene is positioned. The further research finds that the content of C16: 0 can be reduced by the bPLA12B805D gene. Therefore, the invention provides a novel gene capable of reducing the content of rice C16: 0, and a novel method and path are provided for rice breeding.

The invention belongs to the field of molecular biology, provides a functional molecular marker of a major gene AhARF2 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker, discloses a major site qSOCA08-3 for regulating and controlling the oil content of the peanut seed kernels, and explains phenotypic variation of 14.91%-17.31%. The qSOCA08-3 is finely positioned to a physical interval of 349.6 kb between the Tif2. A08.49681404 and the Tif2. A08.50031016, and a candidate gene of the qSOCA08-3 is confirmed to be AhARF2. The qSOCA08-3 has the advantages that the qSOCA08-3 can be used for identifying the The AhARF2 coding sequence shows 275bp deletion between parents Jianhua No.1 and W191 and is accompanied by 7bp replacement, so that the code shift mutation of the coding sequence is caused, and the protein translation is terminated in advance. According to the invention, the KASP molecular marker is developed on the basis of a mutant sequence, the association between the KASP molecular marker and the oil content of the seed kernel is successfully verified in a Yuhua 15 * ST001 recombinant inbred line population, and the important value of the marker in the marker-assisted selection breeding of the high oil content of the seed kernel of the peanut is verified.

The present invention provides molecular markers linked to the major peanut plant-type QTL loci qGH15 and qGH06, and their applications. The molecular markers are Arahy15.SNP and Arahy06.INDEL; the molecular marker Arahy15.SNP is linked to the QTL loci qGH15; the molecular marker Arahy06.INDEL is linked to the QTL loci qGH06. Genotyping in recombinant inbred line (RIL) populations and natural populations using the KASP and PCR methods of the present invention can distinguish upright or loosely upright from semi-running or running. Using this molecular marker-assisted selection can significantly improve the efficiency of selecting for plant-type traits in future generations.

The invention relates to the technical field of agriculture, in particular to a crop phenotype prediction method and a crop phenotype prediction device. The crop phenotype prediction method comprises the following steps: determining the genotype of a parent; determining phenotype data of the parent in the current environment; the genotype of the parent and the phenotype data of the parent are input into a trained prediction model, the prediction value of the phenotype of the crop in the current environment is determined, and the prediction model is obtained through training according to the genotypes of the multiple strains of the recombinant inbred line of the parent and the phenotype data of the multiple strains of the recombinant inbred line in the multiple environments. According to the method and the device, the influence of the genotype of the parent and environmental factors on the phenotype of the crop can be comprehensively considered through the prediction model according to the genotype of the parent and the phenotype characteristics of the parent in any environment, and the predicted value of the phenotype of the crop in the environment is directly determined, so that the phenotype of the crop is predicted more quickly and accurately; the breeding period is effectively shortened, the breeding cost is effectively reduced, and the accuracy and the reliability are relatively high.

The invention relates to the technical field of molecular biology and plant genetic breeding, in particular to application of a QTL (quantitative trait loci) qCsr12 to identification of chilo suppressalis resistance traits of rice. According to the invention, an advanced-generation recombinant inbred line genotype constructed by hybridizing a rice anti-rice-borer variety WFS and an insect-susceptible variety 923 is combined with the insect weight of stem inoculation to carry out genetic linkage analysis, and the anti-rice-borer QTL locus qCsr12 of the WFS in a chromosome 12 is identified and is located between indel1462 and indel1522. The specific molecular marker can be used for effectively detecting the major anti-borer QTL locus qCsr12 in an anti-borer variety WFS and derived varieties thereof, so that the selection efficiency of anti-borer rice is greatly improved, and a rice line containing the major anti-borer locus of the WFS is obtained.

The invention belongs to the field of molecular biology, and provides a functional molecular marker of a major gene AhWRI1 for regulating and controlling the oil content of peanut seed kernels and application of the functional molecular marker. The molecular marker is located at InDel sites of 45bp, 716bp, 738bp-45bp, 718bp and 423bp of a No.8 chromosome of peanuts, the sequence of the molecular marker is SEQ ID NO.1 or SEQ ID NO.2, and a candidate gene of the molecular marker is named as AhWRI1. The KASP molecular marker is successfully developed on the basis of LTR reverse transcription transposon insertion / deletion polymorphism, the association between the KASP molecular marker and the oil content of the seed kernel is verified in a Yuhua 15 * Yueyan 20 recombinant inbred line population and a germplasm resource material, the accuracy of the marker is proved, and the KASP molecular marker can be applied to high-oil-content molecular marker-assisted selection breeding of the peanut seed kernel.

The invention provides application of an upland cotton GhFAM32Al gene in regulation and control of cotton fiber elongation, and relates to the technical field of genetic engineering. According to the invention, a recombinant inbred line group is constructed among parents with significant difference in fiber length and QTL positioning is carried out, so that the GhFAM32Al gene located in the D02 chromosome has significant influence on the cotton fiber length. Gene overexpression and CRISPR / Cas9 knockout means are used for verification and show that high expression of GhFAM32Al in the fiber development stage can remarkably promote fiber elongation, and fiber length reduction is caused by knockout. Further research finds that GhFAM32Al is a nuclear localized natural disordered protein, can form an interaction compound with GhMYBd protein, and synergistically participates in the regulation and control of the fiber cell elongation process. The invention provides a key gene resource and a molecular regulation strategy for high-quality breeding of cotton.