Molecular label closely linked with wangshuibai speces and series wheat tibberellin resistance main efect QTL and its application

A wheat scab and molecular marker technology, which is applied in the field of wheat breeding and molecular biology to improve breeding efficiency and reduce waste.

Inactive Publication Date: 2005-05-18
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And Jiangsu Liyang Wangshuibai (Wheat Scab Research, edited by Lu Weizhong, etc., Science Press, Beijing, 2001) is a unique local variety in my country. It has high and stable scab resistance, and it has also been discovered so far. The wheat variety with the strongest resistance to wheat head blight has no molecular markers linked to its main QTL.

Method used

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  • Molecular label closely linked with wangshuibai speces and series wheat tibberellin resistance main efect QTL and its application
  • Molecular label closely linked with wangshuibai speces and series wheat tibberellin resistance main efect QTL and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The acquisition of molecular markers closely linked to the main QTL for wheat head blight resistance:

[0023] (1) Construction of Wangshuibai recombinant inbred line population and identification of red resistance:

[0024] Crossing Wangshuibai with Alondra, a wheat susceptible to head blight, was used to produce F 1 Hybrid, F 1 Selfing produces F 2 Offspring, F 2 One seed of 104 individual plants was randomly selected for planting, and the method of single seed transmission was used to propagate to F 7 Generation, 104 families were produced.

[0025] Fusarium head blight resistance inoculation identification was carried out in the greenhouse or in the field. The spikelets in the middle of the wheat ears were selected and inoculated with 10 microliters (50,000 spores / ml) of conidia suspension of Fusarium graminearum strain F34. After moisturizing for 3 days, the diseased spikelet rate was investigated after 21 days.

[0026] Diseased spikelet rate=(number of diseased spike...

Embodiment 2

[0046] Molecular markers closely linked to the major QTLs for wheat head blight resistance were verified in high-generation genetic populations:

[0047] The obtained molecular markers closely linked to the main QTL for wheat head blight resistance were also predicted for head blight resistance in some plants of the F9 offspring of Wangshuibai crossed with Alondra. DNA was first isolated from their leaves, and then Use SSR markers Xgwm493, BARC147 and Xgwm161 primers to perform polymerase chain reaction (PCR) on these DNAs, and judge whether the corresponding markers are present or not. The presence of 2-3 markers indicates that the variety or strain of scab Resistance reaches the level of "resistance", if it does not exist, it is susceptible. Then based on these criteria, the head blight resistance of each wheat plant was predicted. Subsequently, the method of inoculating the scab pathogen was used to determine the actual resistance to scab of the tested samples and compared with...

Embodiment 3

[0052] Molecular markers linked to the main QTL for red resistance in Wangshuibai predict the resistance of high-generation breeding materials:

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Abstract

The invention relates to a molecular marker closely linked with the main effect QTL of wheat scab resistance in Wangshuibai variety or strain and application thereof, belonging to the fields of wheat breeding and molecular biology. QTL and genetic linkage analysis of the genotype of each family of the recombinant inbred line obtained from crossing the disease-resistant variety Wangshuibai (♀) and the susceptible variety (♂) and the data of the scab resistance phenotype of each family , and obtained the molecular markers Xgwm161, BARC147 and Xgwm493 closely linked to the major QTL for resistance to Scab in Wangshui. By detecting whether there are molecular markers in the DNA of Wangshuibai and its derivative varieties (lines), the level of resistance to scab can be predicted, thereby speeding up the progress of selection of scab-resistant wheat.

Description

Technical field [0001] The present invention relates to the field of wheat breeding and molecular biology, in particular to selecting and using molecular markers closely linked to the main QTL for resistance to scab resistance in wheat for auxiliary breeding to improve the efficiency of breeding for resistance to scab. Background technique [0002] Wheat head blight is an important disease in warm and humid wheat regions, mainly caused by Fusariumgraminearum. Wheat head blight not only causes serious yield loss, but also mycotoxins such as deoxynivalenol (DON) remaining in diseased wheat kernels seriously threaten the health of humans and animals. Cultivating disease-resistant varieties is the most economical way to control this disease. And effective means. A lot of previous work (Bo Guihua, Shanghai Agricultural Journal, 1989, 5(4): 17-23; Sinijders CHA, Euphytica, 1990, 50: 11-18; SinghRP, Plant Disease, 1995, 79: 238-240; van Ginkel M, Plant Disease, 1996, 80: 863 ~ 867; Bai ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02C12P19/34C12Q1/68
Inventor 周淼平陆维忠张旭任丽娟马鸿翔
Owner JIANGSU ACAD OF AGRI SCI
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