6 results about "Genetic linkage analysis" patented technology

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The invention provides a molecular marker primer closely linked with a brown planthopper resistant major gene Bph48 of rice as well as a marking method and application of the molecular marker primer. A rice insect-resistant variety 75-9-3 (called C268 for short) and Yangdao 9 (called 9311 for short) are hybridized to obtain genotypes of F2 individual plants, genetic linkage analysis is performed in combination with the brown planthopper resistance level of an F3 family, a resistance major gene Bph48 carried by the insect-resistant variety C268 is identified, a Bph48 positioning region is reduced to a 370kb fragment limited by markers 1M30.093 and 1M30.471 on the two sides, and the Bph48 gene is identified to be a Bph48 gene. And the molecular marker is closely linked with the molecular marker 1M30.471. The molecular marker 1M30.471 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C268 and derived varieties (lines) thereof contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper resistant character is greatly improved, and the brown planthopper resistant rice variety (line) containing Bph48 is obtained.

This invention provides a molecular marker primer tightly linked to the major brown planthopper resistance gene Bph57 in rice, along with its marking method and application. This invention utilizes genetic linkage analysis of individual F2 plants obtained from crossing the insect-resistant rice variety “Daomushe Late Bengu” (C9) (♂) with 9311 (♀), combined with the brown planthopper resistance levels of F3 families. This analysis identifies the major resistance gene Bph57 carried by the insect-resistant variety C9. The Bph57 region is narrowed down to an approximately 90kb fragment defined by flanking markers 2M26.442 and 2M26.536, and tightly linked to molecular marker 2M26.762. The molecular marker 2M26.762 of this invention effectively detects whether the insect-resistant variety C9 and its derived varieties (lines) contain this major resistance gene locus, significantly improving the selection efficiency for brown planthopper resistance and obtaining brown planthopper-resistant rice varieties (lines) containing Bph57.

The invention provides a close-linkage molecular marker primer of a rice brown planthopper-resistant major gene Bph55 as well as a marking method and application of the close-linkage molecular marker primer. According to genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety QG44 (sex) and 9311 (sex), genetic linkage analysis is performed in combination with the resistance level of an F3 family to brown planthopper; a resistance major gene Bph55 carried by an insect-resistant variety QG44 is identified, and a Bph55 region is shrunk to a 270kb fragment limited by markers 7M10.726 and 7M10.996 on the two sides and is closely linked with a molecular marker 7M10.726. The molecular marker 7M10.726 provided by the invention can effectively detect whether an insect-resistant variety QG44 and derived varieties (lines) thereof contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper resistant character is greatly improved, and the brown planthopper resistant rice variety (line) containing Bph55 is obtained.

The application relates to the field of corn molecular marker assisted breeding, and particularly discloses application of a Zm00001eb023590 gene in breeding of corn with thick ears, and provides a corn ear thickness molecular marker, the molecular marker is shown as a sequence in SEQ ID NO:1, the application is positioned to a functional gene Zm00001eb023590 for regulating ear thickness by means of GWA analysis and genetic linkage analysis together and located on a chromosome 1, and the functional gene Zm00001eb023590 is mined, the corn ear thickness genetic gene is enriched, a representative molecular marker site in the gene is screened out through haplotype analysis of the gene, redundancy detection cost is reduced in molecular breeding, and meanwhile, the genetic information integrity is reserved.

The invention relates to the technical field of molecular biology and plant genetic breeding, in particular to application of a QTL (quantitative trait loci) qCsr12 to identification of chilo suppressalis resistance traits of rice. According to the invention, an advanced-generation recombinant inbred line genotype constructed by hybridizing a rice anti-rice-borer variety WFS and an insect-susceptible variety 923 is combined with the insect weight of stem inoculation to carry out genetic linkage analysis, and the anti-rice-borer QTL locus qCsr12 of the WFS in a chromosome 12 is identified and is located between indel1462 and indel1522. The specific molecular marker can be used for effectively detecting the major anti-borer QTL locus qCsr12 in an anti-borer variety WFS and derived varieties thereof, so that the selection efficiency of anti-borer rice is greatly improved, and a rice line containing the major anti-borer locus of the WFS is obtained.