Molecular marker of peel full-brown trait gene locus of Chinese 'Qingxiang'-variety pears and screening method of molecular marker

A molecular marker and gene locus technology, applied in the field of molecular genetics, can solve the problems of long cycle and difficulty, and achieve the effect of high cost, cost saving and convenient identification.

Active Publication Date: 2016-02-03
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The brown color of the pericarp belongs to the fruit traits, and the t

Method used

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  • Molecular marker of peel full-brown trait gene locus of Chinese 'Qingxiang'-variety pears and screening method of molecular marker
  • Molecular marker of peel full-brown trait gene locus of Chinese 'Qingxiang'-variety pears and screening method of molecular marker
  • Molecular marker of peel full-brown trait gene locus of Chinese 'Qingxiang'-variety pears and screening method of molecular marker

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Experimental program
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Effect test

Embodiment 1

[0039] The screening method of the molecular marker of the gene locus of the full brown trait of the pear 'Qingxiang' pericarp comprises the following methods,

[0040] A. Using the pear cultivar 'Cuiguan' as the male parent to cross with the pear cultivar 'Qingxiang' to obtain the hybrid F1;

[0041] B. Extract the DNA of each individual plant of the F1 population with the CTAB method, adopt the marker SSR of the simple sequence repeat to carry out polymorphic primer screening to the two parents, amplify by the polymerase chain reaction, and the amplified product is 0.08g / According to the results of molecular marker screening, the polymorphic primers between the parents were screened out, the polymorphic primers between the parents were analyzed in the F1 population, and the polymerase chain reaction was amplified The procedure is the same as above, to obtain population genotype data;

[0042] C. According to the law of linkage exchange, use the population genotype data to ...

Embodiment 2

[0049] The screening method of the molecular marker of the gene locus of the full brown trait of the pear 'Qingxiang' pericarp comprises the following methods,

[0050] A. Using the pear cultivar 'Cuiguan' as the male parent to cross with the pear cultivar 'Qingxiang' to obtain the hybrid F1;

[0051] B. Extract the DNA of each individual plant of the F1 population with the CTAB method, adopt the marker SSR of the simple sequence repeat to carry out polymorphic primer screening to the two parents, amplify by the polymerase chain reaction, and the amplified product is 0.08g / According to the results of molecular marker screening, the polymorphic primers between the parents were screened out, the polymorphic primers between the parents were analyzed in the F1 population, and the polymerase chain reaction was amplified The procedure is the same as above, to obtain population genotype data;

[0052] C. According to the law of linkage exchange, use the population genotype data to ...

Embodiment 3

[0057] The screening method of the molecular marker of the gene locus of the full brown trait of the pear 'Qingxiang' pericarp comprises the following methods,

[0058] A. Using the pear cultivar 'Cuiguan' as the male parent to cross with the pear cultivar 'Qingxiang' to obtain the hybrid F1;

[0059] B. Extract the DNA of each individual plant of the F1 population with the CTAB method, adopt the marker SSR of the simple sequence repeat to carry out polymorphic primer screening to the two parents, amplify by the polymerase chain reaction, and the amplified product is 0.08g / According to the results of molecular marker screening, the polymorphic primers between the parents were screened out, the polymorphic primers between the parents were analyzed in the F1 population, and the polymerase chain reaction was amplified The procedure is the same as above, to obtain population genotype data;

[0060] C. According to the law of linkage exchange, use the population genotype data to ...

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Abstract

The invention relates to the field of molecular genetics and discloses a molecular marker of a peel full-brown trait gene locus of Chinese 'Qingxiang'-variety pears and a screening method of the molecular marker of the peel full-brown trait gene locus of the Chinese 'Qingxiang'-variety pears. A peel full-brown trait and a genotype of each F1 individual plant obtained by hybridization of Chinese 'Qingxiang'-variety pears and Chinese 'Guancui'-variety pears are subjected to genetic linkage analysis to obtain the molecular marker of the peel full-brown trait gene locus Rus.zaas-8 of the Chinese 'Qingxiang'-variety pears. Whether the gene locus is contained in the 'Qingxiang' variety or other derived varieties (strains) or not is detected by means of the molecular marker of the peel full-brown trait gene locus, peel colors of fruits can be predicated, and accordingly selecting efficiency of fruit peel colors of Chinese pears can be greatly improved.

Description

technical field [0001] The present invention relates to the field of molecular genetics, and relates to a molecular marker for the gene locus of the full-brown trait of the pear variety 'Qingxiang', in particular to the screening of the molecular marker for the gene locus of the full-brown trait of the pericarp of the pear variety 'Qingxiang' method. Background technique [0002] Sand pear is an important southern fruit tree in my country. It is a perennial woody plant with a long childhood. Peel color is one of the most important peel properties. Consistent peel color and no rust are important indicators of high-quality commercial pears. The brown color of the pericarp belongs to the fruit traits, and the traditional hybrid breeding method has a long cycle and great difficulty. Generally, it takes 3 to 5 years for the results to carry out preliminary identification. Assisted breeding can effectively solve this problem by locating the gene loci of the brown trait of fruit...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 王月志戴美松施泽彬张树军
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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