Pig 5-aminolevulinic acid synthase 1 gene as genetic marker of pig litter size traits and application thereof

An aminolevulinic acid, genetic marker technology, applied in the application, genetic engineering, acyltransferase and other directions, to achieve the effects of saving production costs, shortening the generation interval, and easy operation

Pending Publication Date: 2020-04-07
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

[0005] At present, because there is no research report on the genetic polymorphism of the 5' flanking region of the pig ALAS1 gene, the present invention has discovered the SNP site in the 5' flanking region of the pig ALAS1 gene, analyzed its relationship with pig litter size traits, and found a relationship with Molecular markers related to piglet size, and a rapid detection method was established

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  • Pig 5-aminolevulinic acid synthase 1 gene as genetic marker of pig litter size traits and application thereof
  • Pig 5-aminolevulinic acid synthase 1 gene as genetic marker of pig litter size traits and application thereof
  • Pig 5-aminolevulinic acid synthase 1 gene as genetic marker of pig litter size traits and application thereof

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Embodiment Construction

[0038] In order to realize the detection of the ALAS1 gene 5' flanking region mutant (SNP), this embodiment has found the SNP site of the pig ALAS1 gene 5' flanking region, analyzed its relationship with pig litter size traits, and found a relationship with pig litter size. Number-related molecular markers, and a rapid detection method was established.

[0039] 1. Collect ear tissue samples and extract genomic DNA

[0040] The ear tissues of local pig breeds (Wannan black pig, Wannan Hua pig, Liubai pig, Huai pig) and introduced pig breeds (Landrace, Large White, Duroc pig) and Huai pig new strain II were collected and their DNA was extracted, and then put into Store in -20°C refrigerator until use.

[0041] 2. Obtaining the 5' flanking sequence fragment of porcine ALAS1 gene

[0042] Using Universal GenomeWalker from Clontech TM The 2.0 Genome Walking Kit constructed four genome walking libraries.

[0043]According to the sequence of the first exon of porcine ALAS1 gene, ...

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Abstract

The invention discloses a pig 5-aminolevulinic acid synthetase 1 gene as a genetic marker of pig litter size traits and an application thereof. The nucleotide sequence of the genetic marker is shown as SEQ ID NO.1, and R at a 26-site base in the sequence is T or C. The mutation causes polymorphism of a 5'flanking region of the porcine 5-aminolevulinic acid synthase 1 gene. According to the invention, the SNP locus of the 5'flanking region of the pig ALAS1 gene is discovered for the first time; the relationship between the SNP locus and the pig litter size traits is analyzed; the molecular marker related to the pig litter size is found; and a rapid detection method is established.

Description

technical field [0001] The invention relates to a porcine 5-aminolevulinic acid synthetase 1 gene as a genetic marker for pig litter size traits and its application. Background technique [0002] The reproductive performance of pigs is an important criterion to measure its productivity, and litter size is the most important trait in reproductive traits from the perspective of genetics and comprehensive economic benefits. However, the low heritability and limited expression of the litter size trait make it almost impossible to genetically improve this trait using conventional techniques. With the application of marker-assisted selection and the identification of major genes and the screening of candidate genes, it has been found that the use of gene selection can double the genetic improvement speed of traits with low heritability and limited traits. There are some reports of candidate genes for litter size traits, but their genetic effects are still unclear, and there are o...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11C12N15/54
CPCC12Q1/6888C12N9/1029C12Y203/01037C12Q2600/124C12Q2600/156
Inventor 刘林清王重龙李庆岗周梅王洁茹
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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