81 results about "Heritability" patented technology

The invention discloses a construction and stock breeding method for cultured grass carp families. The method comprises the following steps of: selecting grass carps which move actively and have high heritability and of which the weight is 15 to 20 percent more than the average weight, as parent fishes; marking cultured grass carps by using periodic interrupt timer (PIT) electronic labels, after sexual gland is matured, facilitating maturation by using hormone solution, performing artificial insemination on female and male parent fishes to obtain oosperm, performing self-hatching, then growing seedlings, breeding fishseed, breeding one-year fishes, feeding respectively, and establishing the families; from the second year to maturation time, injecting the PIT electronic label into the grass carps with the different families; performing mixed culture in a same pond, and eliminating the inferior grass carps, wherein the rest families are the basic families for next generation breeding; the young grass carps, the one-year grass carps, two-year grass carps, three-year grass carps and four-year grass carps are a critical period of family breeding; preferably choosing, and establishing the families serving as backup parent fishes; and then matching teams, raising the seedlings and then breeding continuously. A new product with high quality, high yield and stable yield is bred by establishing the different families of the cultured grass carps; and the method is used for researching the genetic characteristic and breed differentiation conditions by mating the male and female fishes among the grass carp families and inbreeding the male and female fishes in the grass carp family.

The invention discloses a docosahexaenoic acid-producing bacterium and a mutation and screening method and application thereof. The strain is classified and named as Crypthecodinium cohnii HX-308, is preserved in China Center for Type Culture Collection, and has the preservation number M208246. The strain is a DHA-producing bacterium with high yield, has the DHA content 1.53 times of a starting strain, stable physiological-biochemical characteristic and stable heritability, and simultaneously can produce polyunsaturated fatty acid through liquid culture and fermentation; the content of unsaturated fatty acid reaches 20.4g / L, wherein the DHA content reaches 8.16g / L; and the strain is a good strain for producing natural DHA, and has excellent industrial production prospect.

The invention discloses a method for utilizing machine learning to predict a complex disease susceptibility locus. The method comprises the following steps of 1, collecting a known complex disease susceptibility locus as a positive set of a machine learning model, predicting a locus irrelevant to a complex disease according to the positive set as a negative set, and annotating an epigenetic regulation element; 2, utilizing machine learning to establish a complex disease epigenetic model; 3, predicting all loci in a whole-genome range according to the established model to obtain a final prediction result as a potential susceptibility locus of the complex disease. According to the method for utilizing machine learning to predict the complex disease susceptibility locus, epigenetic information and genome DNA information are combined, epigenetic element features are extracted through machine learning, the susceptibility locus of the complex disease is further predicted in a whole-genome range, heritability explained by the found susceptibility locus can be obviously improved, and a potential target is provided for subsequent medicine design and disease detection.

The tissue culture method of Chinese yam includes obtaining bud block as explant, sterilizing bud block, inoculating, provisional planting in greenhouse and other steps. The present invention has the innovations of using bud block as explant to result in less virus, high inoculation efficiency, stable heritability, etc.; improved tissue culture program including inducing rooting, cutting the base part of the bud seedling, soaking in NAA solution of 50 ml / L concentration, inserting in disinfected fine sand for provisional planting and applying diluted MS solution and nutrients and resulting in decreased comprehensive production cost of tissue culture. The present invention makes it possible to realize large scaled production of excellent tissue culture seedling of Chinese yam.

The invention discloses multi-character selection breeding method of fish and shrimps. The method comprises the following steps of: firstly obtaining individual selection indexes and family selection indexes according to measured values of breeding target characters, then reserving stocks and hybridizing according to the selection indexes, building family mating groups and finally obtaining bred populations of the next generation. Moreover, when the individual selection indexes are obtained, the stock reservation rate and the heritability of breeding target characters are firstly calculated according to the measured values of the breeding target characters, expected selection intensity is calculated according to the stock reservation rate, then an expected selection response and a corresponding weighted value of each breeding target character are obtained according to the expected selection intensity, the heritability and the standard difference of a standardized breeding value, and finally the individual selection indexes are obtained by using the weighted value. Therefore, the subjectivity and the randomness during calculation of the weighted value are eliminated, the reliability and the accuracy of the breeding method are high, and the comprehensive genetic progress of multi-character selection is improved.

The invention discloses a high-quality paternal chicken breeding method. A paternal circulation breeding method is adopted. A certain quantity of hens and cocks are adopted for breeding. Time of breeding high-quality paternal chicken is reduced. Only 2-3 years are required for breeding the high-quality paternal chicken while 10 years are usually required for standardized breeding. In the breeding process, in combination with artificial breeding and screening, characters of individuals can be highlighted, the individuals with excellent characters can be conveniently selected for breeding, the paternal performance can be obviously improved, the breeding progress is accelerated and the appearance characters are obviously improved. By adopting a method of selecting individuals and increasing selection pressure for characters with high heritability, new lines can be formed within short time.

The invention discloses a restrictive two-stage genome-wide association study (GWAS) method based on an SNPLDB mark, and aims at solving the problems that a traditional method cannot be used for estimating multiple allele information, and is high in false positive rate and low in efficiency of detection on inbreeding crops. By combination of the SNPLDB mark constructed on the basis of a haplotype block, correction of deviation of an inbreeding population relation analysis model and a two-stage relation analysis strategy under a multi-site model, the GWAS method suitable for conventional breeding of inbreeding crops is built; the SNPLDB mark is applied to GWAS, so that a method is provided for multiple allele estimation; candidate sites are screened on the basis of a single-site model in the first stage, and are further screened on the basis of a progressive regression analysis method in the multi-site model in the second stage, so as to balance the problems of missing of heritability and over-high heritability estimation. Therefore, the interpretation ratio of a final genetic model is controlled at trait heritability. The positioning accuracy and efficiency are improved by a feature vector and an appropriate significant level of a similarity coefficient matrix estimated by the SNPLDB mark by GWAS.

The invention relates to a breeding method of a brassica napus radish cytoplasm sterile restorer line. Through brassica napus radish cytoplasm sterile restorer material CLR650 whole genome resequencing data and radish genome sketch information comparison, specific molecular markers of linked exogenous radish fragments and restoring genes in CLR650 are developed; then, a target single strain of the brassica napus radish cytoplasm sterile restorer material CLR650 backcross population is bred in an auxiliary way through the specific molecular markers; a BC4 separation group is obtained; fertile plants in the BC4 group is subjected to multi-generation mixed selection by a mixed method until the mixed separation group with the fertile plant proportion exceeding 75 percent is obtained; finally, the homozygosis stabilization is performed through multi-generation selfing; the novel brassica napus radish cytoplasm sterile restorer line is obtained. The method provided by the invention overcomes the defects of too low restoring gene heritability and property homozygosis stabilization difficulty of the existing method.

The invention discloses an algorithm for assessing heritability through genome data. The algorithm comprises the steps that for a certain quantitative character, marker effect estimation is conducted on a whole genome with different numbers of reference group individuals through a GBLUP algorithm, the breeding value of an estimated group is further obtained, and estimation accuracy is calculated; curve linearization fitting is conducted through genome estimation accuracy and the size of reference groups, and the reciprocal of the intercept of a regression equation obtained through fitting serves as the estimated value of heritability. According to the algorithm for assessing heritability through the genome data, heritability of the quantitative character is assessed through the genome data, the research achievement can be directly applied to quantitative character breeding of animals and plants, genealogy recording is not conducted on the individuals, but sequencing is conducted on each individual genome, heritability of the character is predicted through whole genome marks, the heritability estimation result is mainly applied to breeding work in the future, in addition, Mendel sampling errors can be captured through sequencing, and compared with genealogy data recording, more accurate genealogy information can be obtained.

The invention discloses a seed quantitative trait locus positioning method based on a mixed linear model. The method comprises the step of establishing a statistics genetic model, the step of searching the range of a whole genome for all candidate label sections in which an OTL probably exists, the step of regarding the candidate label sections as a concomitant variable, and searching the range of the whole genome for obvious QTLs and a two interaction label section, the step of regarding the QTLs and the two interaction label section as the concomitant variables and searching an obvious interaction label section for obvious loca of a two interaction epistasis, and the step of obtaining coefficients of various effects in the statistics genetic model based on the QTLs and the loca of the two interaction epistasis and calculating the effect of the loca and estimating the heritability of all loca according to the statistics genetic model. The seeds are divided into diploid seeds and triploid seeds according to the traits of the seeds, the epistasis effect and the effect of interaction of genes and the environment are taken into consideration, and the positions of the seed quantitative trait loca and various effects can be fast estimated in an unbiased mode.

The invention discloses a dog parvovirus attenuated vaccine strain and application thereof. In the invention, the separated dog parvovirus virulent strain is cultivated in a cell subculture mode to obtain the dog parvovirus attenuated vaccine strain. The microbial preservation number is CGMCC No.3841. The test on safety and immunogenicity indicates that the attenuated vaccine strain CPV-YNR can protect the dog subjected to the attack of homologous virulent strain and has favorable immunogenicity. The attenuated vaccine strain CPV-YNR can be used for preparing a single vaccine or combined vaccine, and can effectively prevent or treat diseases caused by dog parvovirus. The attenuated vaccine strain CPV-YNR has the advantages of stable heritability, enduring immunity, favorable effect, high safety and reliability, long storage time and the like.

The invention discloses a method for improving the lateral branch rooting survival rate and the seed reproduction quality of radish. The method comprises the following steps: a second-stage lateral branch is taken as a cutting branch, after the cut-off lateral branch heals naturally, the cut-off lateral branch is soaked in an indolebutyric acid solution for 10 seconds and then inserted in a seedbed substrate composed of coco coir, vermiculite and mushroom dreg; a mixed solution containing Huabao No.1, Huabao No.2 and naphthylacetic acid is sprayed on the branch so that the branch can be acclimated and then survives; when new leaves come out, transplanting is performed by use of an underground simple culture tank filled with planting soil which is composed of coco coir, vermiculite, mushroom dreg and decomposed cow dung; after the cutting lateral branch survives, ventilation and light illumination are realized, Huabao No.1 and Huabao No.2 are sprayed, and after seeds are mature, the seeds are harvested. The method for improving the lateral branch rooting survival rate and the seed reproduction quality of the radish has the advantages that the method of rooting by use of the second-stage cutting lateral branch of the radish is adopted, a new method is provided for solving the problem of seed reproduction material loss of the radish due to the infringement of soft rot, the plants cultured by use of the method are well developed in root system, high in transplanting survival rate, and stable in heritability, and furthermore, the seed reproduction can be saved.

The invention discloses a breeding method for a shellfish meat trait new strain. The breeding method comprises the following steps: firstly constructing more than 20 full-sib and / or half-sib families, and breeding all the families in different sea areas; performing meat trait index measurement and apparent index measurement on all the bred families; then screening out meat trait indexes with the heritability larger than 0.2, and screening out indexes with the genetic correlation absolute value larger than 0.5 between the meat trait indexes and the appearance indexes; screening out candidate parent families according to the retention rate of 20 percent, and selecting optimal individuals from the candidate parent families to be used as reproduction parents according to the appearance trait indexes; finally carrying out generational breeding according to the breeding method, and finally forming the new strain with high meat trait index. According to the breeding method, on the premise that a research object is not dissected, a method of combining family selection and within-family selection is adopted for breeding the meat trait index from the directly measurable appearance indexes by indirect breeding.

The invention relates to a method for breeding a two-point-pattern lacy duck new breed. The method comprises the following steps of: 1) selecting Sichuan lacy ducks as materials and inbreeding two generations, wherein the Sichuan lacy ducks are hybridized by white feather Tianfu meat male ducks and Sichuan local female sheldrakes; 2) hybridizing male ducks obtained in the step 1) with female cherry valley ducks to generate hybrid lacy ducks, performing appearance selection for three generations, and building the hybrid lacy duck F1 generation by the remained individuals; 3) performing early breeding on the hybrid lacy duck F1 generation by using a molecular marker of an MC1R gene; 4) performing family breeding on the obtained remained individuals and performing closed breeding to generate F2 generation; and 5) hybridizing the F2 generation individuals with Aobaixing Duck or Beijing ducks to generate the lacy ducks with a small amount of black feather on the heads and the tails. The method has the advantages that: hybrid combination is performed on the basis of natural group lacy ducks; and the meat duck new breed with consistent appearance and stable heritability are bred by the traditional breeding method and the modern molecular breeding method.

The invention discloses a method for inducing gynogenetic diploid of grouper, which comprises the following steps of: diluting semen of the grouper, and radiating the semen by using ultraviolet rays to make the heritability of the semen deactivated; mixing with mature eggs of the grouper; after the eggs are fertilized, transferring to seawater for cold shock treatment to double genomes and generate the gynogenetic diploid; and transferring to seawater, and managing according to common grouper fry to obtain the gynogenetic diploid of the grouper. The offspring generated by induction only contains the gene of the female parent, the homozygosis of good genes can be quickened, and good hereditary features are quickly fixed; and the method can be applied to genetic analysis of the grouper, and has wide application prospect in the aspects of culture and breeding of the grouper.

The invention discloses a serum biochemical marker for auxiliary screening of low-fat meat chickens and application of the serum biochemical marker, and belongs to the technical field of genetic breeding of poultry. The serum biochemical marker provided by the invention is the HDL-C / LDL-C level in chicken serum. Such serum biochemical markers in low-fat meat chickens and high-fat meat chickens have significant differences, also have high genetic correlation coefficients and heritability and can be used for identification of abdominal fat of meat chicken and breading of low-fat meat chickens. Besides, the invention further provides application and a screening method of the serum biochemical marker.

The invention discloses a gene mutation site related to reproduction traits of Hu-sheep and application thereof. The mutation site is located at Hu-sheep FSHR gene core promoter region-414 site nucleotide; A>G mutation is located at the site and is obviously related to the lambing quantity of the Hu-sheep, wherein AA and AG type individual lambing quantity is obviously higher than the GG type individual lambing quantity; the genetic transcription activity in AA type promoter region is obviously higher than that of GG type promoter region. The livestock reproduction trait is a low heritability trait; the genetic progress is slower when the conventional breeding technique is adopted; the mutation site is obviously related to the reproductive performance of Hu-sheep, so that the high-fecundity genotype individual is remained and the low-fecundity genotype individual is abandoned by adopting a simple genetic typing technique for detecting the site; the gene mutation site has an important economic value for quickening the breeding process of the high-fecundity Hu-sheep colony or strain.

The invention relates to the technical field of biology, in particular to a silkworm Bmlp3 gene promoter represented by SEQ ID No.1. The promoter can be used for expressing an extrinsic protein in a fat body. The invention also comprises a preparation method of a microscopic injection carrier for expressing the extrinsic protein in the fat body of silkworms, which comprises: constructing a recombinant expression vector containing a silkworm Bmlp3 gene promoter and an expression cassette; and preparing the microscopic injection carrier. The Bmlp3 gene promoter can realize the expression of the extrinsic protein in a development period in which the fat body is the most developed. The method can realize the controllability of an expression period; the transgenosis-mediated by a transposons derived from pggBac has characteristics of high stability, high heritability and the like; and through continuous rearing, colony can be enlarged quickly, and a quantitative guarantee for purification of the EQ ID No.1. The promoter can be used for expressing an extrinsic protein is provided.

The invention relates to a selection method capable of simultaneously improving feed efficiency and increasing growth speed of pigs. The selection method is characterized in that the selection methodfor simultaneously improving the feed efficiency and increasing growth speed of pigs by utilizing over-growth body weight traits comprises the steps of selection of basic conditions, determination ofindexes and data processing, breeding value estimation, comprehensive selection index calculation, and reserve breeding pig selection, matching and verification. The selection method is simple and practical, is good in selection effect, can be used for phenotypic selection of simultaneous determination groups, can be used for comparing and selecting different determination groups according to heritability and genetic correlation, and can be used for directly selectively improving the feed efficiency and increasing the growth speed of pig groups.

The invention discloses a molecular marker related to skin color of chicken, a primer group and application, and relates to the technical field of poultry genetic breeding. The invention provides an SNP related to the skin color of the chicken. The nucleotide sequence of the SNP is shown as SEQ ID NO: 1. The invention also provides a specific primer group for detecting the SNP and a specific detection method, which can detect the SNP sequence efficiently, sensitively and quickly so as to identify the skin color. The SNP site disclosed by the invention is unique, has very high broad-sense heritability, can accurately identify the color of the chests or legs of the broiler chicken after their molt, and improves the consistency of the skin color and the shin color of offspring chicken flocks of the broiler chicken.

The invention discloses a hybrid wheat breeding method. By a breeding way of combining low-temperature germination culture with hybridization, wheat seeds which still can be germinated at a temperature of 4 DEG C are screened out, and then are hybridized with a high-yield stable-yield female parent; successively, low-temperature germination culture and hybridization are continuously carried out; after secondary hybridization, offsprings with excellent characters are formed by selective breeding for inbreeding until a new variety of wheat with stable heritability and high combining ability is obtained; a new wheat breeding method is developed, indoor germination treatment on wheat seeds is carried out by using a gradually cooling stress method, the wheat seeds with strong freezing resistance are screened out, and the damage of low temperature to the wheat seeds is reduced; the finally obtained new wheat variety has good freezing resistance, and the problems that the operation of a molecular breeding method is trivial and the yield cannot be increased are solved.

The invention discloses a breeding method of drought-resistant high quality millets. The breeding method adopts a flood and drought parallel and pedigree technology combined continuous directional breeding technology to clarify the property screening criteria of every breeding generation in order to obtain the drought-resistant high quality millets. The breeding method is adopted to directionally breed seven consecutive generations of a millet hybrid progeny and carry out specific breeding and property breeding combination on different progenies according to the variation range of the high yield, the drought resistance and other properties and other heritability characteristics of the millets in order to cultivate a new millet specie with high drought resistance, nourishing and high quality in the whole growth period based on excellent comprehensive properties.

The invention relates to a cultivation method capable of increasing the germination rate of Chinese prickly ash and belongs to the technical field of crop planting. The cultivation method includes: producing seeds, to be more specific, selecting a seed source region, selecting seed Chinese prickly ash trees, picking the Chinese prickly ash trees, processing seed Chinese prickly ash, storing the seeds, and degreasing the seeds; sowing, to be more specific, selecting a nursery field, preparing the nursery field, sterilizing the nursery field, fertilizing the seedbed, sowing the Chinese prickly ash seeds, building an arched shed, and managing seedlings. The method has the advantages that the method is developed according to years of planting researches and grope, the seed production and sowing methods are unique, the germination rate of the cultivated Chinese prickly ash reaches up to about 85%, the germination rate of the method is increased by 70% as compared with that of a traditionalmethod, the Chinese prickly ash seedlings cultivated by the method are developed in root system, high in survival rate, high in drought resistance, cold resistance and disease and pest resistance andstable in heritability, and the produced Chinese prickly ash is large in size, good in quality, high in yield and evident in economic benefit.

The invention relates to a curdlan and a preparation method thereof. Agrobacteriumsp.fcynl with the preservation number of CGMCC5103 is taken, and goes through strain activation, primary seed culture, secondary seed culture, bacteria culture, fermentation, first-time centrifugation, second-time centrifugation, third-time centrifugation and other steps, and after drying, the curdlan is harvested. In the method provided by the invention, the Agrobacteriumsp.fcynl strain is utilized to produce the curdlan, the yield can reach 34.5g / L, and is increased by 97.3 percent in comparison with the priorart, moreover, the fermentation period is short, the heritability is stable, the cost is saved, and the price is low.

The invention discloses a breeding method for drought-resistant high-quality millet. The method adopts a continuous directed breeding method combining parallel floods and droughts with a pedigree method to define character screening criteria for each breeding generation so as to breed a variety of drought-resistant high-quality millet. The technical scheme disclosed in the invention is as follows:the continuous seven-generation directed breeding of millet hybrids by using the method disclosed in the invention is performed, and based on the high yield, drought resistance and variation range and heritability of other characters of the millet, the combination of specific breeding methods and breeding characters is conducted on the different generations, so that new millet varieties with thehigh drought resistance and high nutrition quality are bred on the basis of excellent comprehensive characters.

The invention relates to the field of plant tissue culture, in particular to a phalaenopsis amabilis tissue culture propagation method. The phalaenopsis amabilis tissue culture propagation method comprises the following steps of induction of pedicel buds, differentiation and proliferation of cluster buds, shoot strengthening and root induction, seedling hardening and transplanting. An induction culture medium is prepared from mashed potatoes and mashed bananas, and saccharose is replaced by white granulated sugar, so that the production cost of large-scale proliferation of phalaenopsis amabilis can be greatly reduced, and the growth situation is good; coconut milk in a differentiation and proliferation culture medium is also one of key factors of proliferation, and the proliferation rate, the proliferation time and the economic benefit are the best when the content of the coconut milk is 10 percent. By adopting the phalaenopsis amabilis tissue culture propagation method of the technical scheme, tissue culture and rapid propagation are carried out along a path of the cluster buds of the phalaenopsis amabilis, the phalaenopsis amabilis tissue culture propagation method has the advantages of high inductivity, short induction time, high proliferation rate of the cluster buds, low technical difficulty and stable heritability, a large amount of variation can be prevented from happening during production, and a great significance in large-scale production is obtained.

The invention discloses a selection method of Taihu pigs and belongs to the field of animal breeding. Reciprocal cross is performed on measly pigs, Meishan pigs, Fengjing pigs and Erhualian pigs from the TaiHu lake area, thus heterosis is expanded, a breeding line of Meishan pig-measly pig-Erhualian pig-Fengjing pig is established, and a breed which is high in reproductive performance and stable in heritability is selected. Compared with the Taihu pigs without hybrid cross such as Meishan pigs, the Taihu pigs subjected to the reciprocal cross are high in total number born and number born alive, the defect brought by selfing of the Taihu pigs is overcome, the heritability of the reproductive performance of local pigs in China is maintained, and a special high-quality female parent specialized line is provided for pig production and breeding industry.

The invention provides Vero clone V-Mito of red fluorescence specificity marking mitochondria. Ultrapure plasmid pDsRed2-Mito transfects Vero cells through lipidosome LipofectamineTM2000 mediate, and is carried out with masculine clone screening through a culture medium with G418 so as to select masculine clones. The masculine clones are enlarged, cultured and built with an organ named V-Mito. The cellular organ mitochondria of the cells can steadily give out red fluorescence under laser light, and the specificity shows the shape of the mitochondria. The cellular organ V-Mito of red fluorescence specificity marking mitochondria has no damage to the cells, does not affect the biology activity of the cells, has steady heritability, can reproduce for many times, can proliferate without limitation, and has the advantages of convenient use and low cost. The cellular organ V-Mito has wide application prospects under normal and abnormal physiological status of cells, and under pathogenicbacteria or virus infection state on research aspects.

The invention relates to a breeding method of a pinctada martensi black-shell strain for sea pearl cultivation and belongs to the technical field of pearl breeding. The breeding method includes taking two geographical populations, namely the wild population of Lingshui of Hainan and the cultured population of Leizhou of Beibuwan as the breeding base populations, selecting high-quality individualities as crossing parents from the two populations , breeding progeny by means of reciprocal cross and selfing of different geographical populations, and selecting the progeny of the combination of 'leizhou *lingshui' with remarkable growth superiority from the progeny to perform enclosed breeding of population so as to obtain a certain amount of the pinctada martensi black-shell strain with obvious feature of black shells, good economic character and stable heritability. The breeding method has the advantages of feasible principle, simple processes and low production cost, can improve the survival rate, growth rate, pearl quality and output of the pinctada martensi greatly, and accordingly can improve sustainable development of the national sea pearl cultivation field.