36 results about "Meishan pig" patented technology

It relates to a method of cultivating and breeding new animal species, and especially a method of cultivating and breeding new pig species. The method includes the steps as below. First, domesticating the boars, creating the Domestication environment and feeding the boars with favorite feeding stuff. Second, selection of boars, that is, through genetic analysis, selecting the Duroc and the Meishan pig as the pig races of mating cultivate agnate female parent and matrilineal maternal. Third, mating and breeding, that is, across mating fixedly the young animal generation, and selecting and seeding for generations. Four, Rearing technique, that is, feeding with green grass and roughage principally.

The invention provides an SNP (single nucleotide polymorphism) marker combination and identification method of a small Meishan pig and a raw meat product. The method comprises the following steps: extracting a genome DNA of raw pork or a meat product, performing AGE (agarose gel electrophoresis) and Sanger sequencing after performing PCR (polymerase chain reaction) amplification on the genome DNA,and identifying the small Meishan pig and the meat product thereof according to an SNP genotype of a characteristic site of a sequencing result, wherein in the step of Sanger sequencing, peculiar mutation occurs at identified sites: SNP6, SNP12, SNP16, SNP71 and SNP79. According to the method, the problem that an identification method of the small Meishan pig and the meat product thereof does notexist in the prior art is solved.

The invention aims to provide a detection method for searching the genetic mutation sites of porcine MMP23 and the genetic polymorphism, namely, the molecular marking method for speculating and identifying the litter size with MMP23. The invention provides the sequence of genomic nucleotides with a length of 482bp including the exons 2, 3 and 4 and the introns 2 and 3 of the porcine MMP23 gene of large white sows, Meishan pigs and Qingping pigs, and provides the single nucleotide polymorphism (SNP) of at two sites situating in the interior of the proliferated 482bp-long segment. The method provided by the invention can be used for developing diagnostic procedures or kits used for selecting the pigs carrying the advantageous alleles in breeding programs with better selective outcome.

The invention discloses a cross-breeding method for commercial pigs possessing Meishan pig blood lineage and Berkshire pig lineage. The cross-breeding method comprises the steps of cross-breeding Meishan pigs with Berkshire pigs to breed commercial pigs, or cross-breeding later generations bred by Berkshire boars and Meishan sows with the Meishan boars to breed commercial pigs. The Berkshire pig lineage of foreign pig variety is introduced to appropriately improve the growth speed and lean meat percentage of the cross-bred commercial pigs while an excellent meat quality characteristic and a reproductive characteristic of the Meishan pigs are kept. The growth speed of the commercial pigs is 480 g-600 g every day, the lean meat percentage of each slaughtered pig which is about 80 kilogram ranges from 45% to 55%, the feed conversion rate is 3.3-3.8: 1, the meat color grade ranges from 3.0 to 4.5, and the intramscular fat content ranges from 3.5% to 5.0%.

The invention discloses a method for breeding a high-quality pig complete set line by aid of Tibetan pigs. The method includes steps: (1) the Tibetan pigs and Meishan pigs serve as breeding materials to breed a synthetic line, namely a Tibetan-Meishan synthetic line, and the Tibetan-Meishan synthetic line serves as female parents of the complete set line; (2) Berkshire pigs serve as the first male parents of the complete set line, the Tibetan-Meishan synthetic line serves as the female parents, and sows of the parents' generation of the complete set line are obtained after hybridization; (3) durocs serve as terminal male parents to be hybridized with the sows of the parents' generation to obtain high-quality commercial pigs of the complete set line. Pigs in the complete set line bred in the method are identical in body forms and appearances, hair on the whole pig bodies is black, and brown pigs and white pigs are few; heads of the pigs are light, mouths of the pigs are straight, and ears of the pigs are median in size; backs and bellies of the pigs are flat and straight, body and limb combination is good, and legs and buttocks are well developed. The meat quality is good, production efficiency is high, weight of the pigs can reach 90 kg at 187.22 days of age, the feed conversion rate is 3.14, the lean meat percentage is 57.72%, and the intramscular fat content is 4.07%.

The invention discloses a commercial pork pig multiple crossbreeding method. The method comprises the following steps of A) taking Pietrain pig with non-halothane allogene as the first male parent of multiple crossbreeding and Meishan pig as the first female parent of multiple crossbreeding for hybridization to obtain Pietrain-Meishan-crossed hybrid pig; B) taking Duroc pig as the second male parent of multiple crossbreeding and the Pietrain-Meishan-crossed hybrid pig as the terminal female parent of multiple crossbreeding for hybridization to obtain commercial pork pig. According to the commercial pork pig multiple crossbreeding method, the average litter size of multiparity sow of the first female parent of multiple crossbreeding is not less than 14.5; the hybrid high-quality commercial pork pig obtains an average daily gain up to more than 680 g/d (gramme per day) and a lean meat rate up to more than 64% and a feed conversion rate as little as 2.75, avoids PSE (pale-soft-exudative) meat and obtains an intramuscular fat percentage up to 2.5%.

The invention belongs to the technical field of pig molecular marker preparation and particularly relates to a genetic marker using pig MLC(myosin light chain)2 5' flanking promoter segments as pig carcass traits as well as a preparation method and application of the genetic marker. The genetic marker has the nucleotide sequence shown as SEQ ID NO (sequencer identifier number):1 and SEQ ID NO:2 in a sequence table. One A/G mutation and one G/A mutation respectively exist at the 53bp part of the sequence shown as SEQ ID NO:1 and SEQ ID NO:2, and the polymorphism of DraIII-RFLP is caused by the allele mutation. The genetic marker is used for carrying out pig carcass traits correlation analysis on large white pig and meishan pig F2 generations, and the transcriptional activity of promoters in different genotypes is detected. The invention also discloses a parting detection method of the genetic marker, and the novel genetic marker and the detection method are provided for the pig carcass traits molecular marker auxiliary selection.

The invention discloses a selection method of Taihu pigs and belongs to the field of animal breeding. Reciprocal cross is performed on measly pigs, Meishan pigs, Fengjing pigs and Erhualian pigs from the TaiHu lake area, thus heterosis is expanded, a breeding line of Meishan pig-measly pig-Erhualian pig-Fengjing pig is established, and a breed which is high in reproductive performance and stable in heritability is selected. Compared with the Taihu pigs without hybrid cross such as Meishan pigs, the Taihu pigs subjected to the reciprocal cross are high in total number born and number born alive, the defect brought by selfing of the Taihu pigs is overcome, the heritability of the reproductive performance of local pigs in China is maintained, and a special high-quality female parent specialized line is provided for pig production and breeding industry.

The invention discloses a traditional Chinese medicine feed additive for improving immune function of pigs as well as a preparation method and application of the additive. The additive is prepared from the following components in parts by weight: astragalus membranaceus, fructus alpiniae oxyphyllae, Chinese yam, endothelium corneum gigeriae galli, poria cocos, hawthorn, pinellia ternate, rhizoma atractylodis, ginger, fructus amomi and liquorice. The preparation method comprises the steps of drying, smashing, screening and uniformly mixing the raw materials by adopting a traditional Chinese medicine production device to prepare, adding a prepared traditional Chinese medicine preparation into basic ration of a small healthy Meishan pig of 60 days, uniformly blending to feed, and carrying out free choice feeding and free water drinking. Upon verification of tests, the method can obviously improve the concentration of total protein and albumin in porcine serum; can significantly improve the content of immune globulin IgG and the content of a complement C3 in the porcine serum; obviously improves the antibody level of post-inoculation swine fever; increases the pig spleen indexes. Moreover, the defects of various chemicals and antibiotics feed additives are overcome, toxic and side effects and drug residues do not exist, and the body immune function is improved while the production of green and high-quality animal products is guaranteed.

The invention particularly relates to separation identification for a fusion promoter efficiently expressed in pig muscular tissues. According to the invention, cloning deletion segments of 4 different promoters on the upstream of an MEF2C gene of a Meishan pig, constructing the segments into a pGL3-Basic carrier and confirming the activity, wherein the activity of pGL3-MEF2CP3 is relatively stronger, and the nucleotide sequence of the promoter is as shown in SEQ ID NO:1. By replacing a luc segment of the pGL3-Basic carrier to be in sequence of PPAR gama CDS, the nucleotide sequence is as shown in SEQ ID NO:2 to construct a fusion promoter carrier pGL3-MEF2CP3-PPAR gama; the expression of the PPAR gama is improved through the verifications of both semiquantitative PCR and protein imprint.

The invention belongs to the technical field of animal estrus monitoring, and particularly relates to a meishan pig estrus monitoring method based on acoustic information. The method comprises the steps that a meishan pig estrus acoustic signal and the sound made by the sniffling, eating, screaming, mumbling and ear moving of other pigs in a pig farm environment are firstly collected, pre-processing is conducted on the pig acoustic signal through multi-window spectrum Wiener speech enhancement based on wavelet thresholding and double-threshold end-point detection based on short-time energy, and the denoising and effective signal detection of pig acoustic signal are achieved; the time-and-frequency domain features of the pig acoustic signal are analyzed by using a time-and-frequency analyzing method and a short-time analyzing technology, 1352-dimension of meishan pig acoustic features in total of 300-dimension short-time energy, 300-dimension short-time zero crossing rate, 32-dimensionsub band frequency range energy ratio based on six-layer wavelet decomposing and 720-dimension Vermeer cepstral coefficients which are standardized by a time wrapping algorithm are extracted; the recognition of meishan pig estrus sound performed by a depth belief network (DBN) is constructed. The method has the advantages that the estrus period of the meishan pig is accurately judged to help the timely conception of sows, achieve the propagation potential of the sows and improve economical benefits.

The invention relates to a method for screening candidate genes related to litter size of sow by using a proteomics technology, and discloses a method for screening candidate genes related to litter size of the sow. With the present invention, a differential display is adopted for searching differential proteins in ovaries of specific heavy producing pig breeds of Meishan pigs in China and sus scrofa with low litter size, in particular, over-expression genes in the ovaries of the Meishan pigs, to quickly identify the genes related to the litter size of the sow a large scale. Compared to the previous method for screening the candidate genes, the method provided by the present invention is quick and exact; the method can be combined with a transgenic animal technology so as to provide motive power for breed of new transgenic variety and industrialization in China.

The invention discloses a black pig breeding method. The black pig breeding method comprises the following steps that firstly, a primary group is selected, a purebred Meishan pig is selected as a female parent and a purebred Tibetan pig is selected as a male parent, hybridizing is carried out, and after the Meishan pig female parent farrowing, a sow piglet is selected as a first-generation female parent for later use; secondly, a second-generation group is selected, the first-generation female parent piglets in the first step are bred to an estrus period, purebred Wujin pigs are selected as male parents, hybridizing is carried out, and sow piglets are selected as second-generation female parents for later use after the first-generation female parents give birth; thirdly, breeding pigs are selected, purebred Duroc pigs are selected as male parents for hybridization when the second-generation female parent piglets in the second step are fed to the estrus period, and the piglets are taken as the breeding pigs after the second-generation female parents give birth; and fourthly, the breeding pigs are bred, and in the breeding process, the breeding pigs are fed with feed three times. The piglets bred through the breeding method are high in growth speed and high in disease resistance, meanwhile, the feed is used for feeding the piglets, growth of the piglets can be promoted, and the feeding amount and the disease resistance of the piglets are improved.

The invention belongs to the technical field of cross breeding, and particularly relates to a method for breeding special wild boars by crossing wild boars with domestic pigs. The method comprises the specific steps that 1, crossing is conducted by taking pure south China wild boars as male parents and taking pure Xinjiang wild boars as female parents to obtain F1-generation crossed wild boars; 2, crossing is conducted by taking the F1-generation crossed wild boars as male parents and taking pure Meishan pigs as female parents to obtain F2-generation crossed pigs; 3, crossing is conducted by taking the pure south China wild boars as male parents and taking the F2-generation crossed pigs as female parents to obtain F3-generation crossed pigs; 4, athwart crossing is conducted on the F3-generation crossed female pigs and the F3-generation crossed male pigs to obtain an athwart crossing generation; 5, the athwart crossing generation is subjected to 3-4 generations of breeding. The special wild boars bred through the method are good in reproductive performance and high in piglet survival rate.

The invention relates to an IncRNA marker related to bacterial diarrhea resistance of piglets and application of the IncRNA marker. According to the IncRNA marker and the application thereof, a key candidate lncRNA-FUT3-AS1 for Escherichia coli F18 infection regulation is screened through omics sequencing, the sequence is SEQ ID NO: 1, and on the basis, deep analysis is carried out around the function and regulation mechanism of the FUT3-AS1 in the research; firstly, qPCR and Northern blot technologies are used for detecting the expression difference condition of the FUT3-AS1 in duodenums of F18 Escherichia coli sensitive and resistant weaned piglets of Meishan pigs and Sutai pigs, lncRNA-FUT3-AS1-silenced pig small intestine epithelial cells are successfully established, and the interference efficiency reaches 68%; and a series of detection means such as bacterial counting, Gram's staining, scanning electron microscopy and indirect immunofluorescence find that the adhesion capability of F18 Escherichia coli to IPEC-J2 cells can be remarkably lowered by silencing lncRNA-FUT3-AS1. Therefore, the lncRNA-FUT3-AS1 plays an important resistance regulation function in a process of resisting F18 Escherichia coli infection of the weaned piglets, the adhesion ability of the small intestine epithelial cells to the F18 Escherichia coli is lowered by down-regulating an expression level of the lncRNA-FUT3-AS1, and the resistance ability of the piglets to the F18 Escherichia coli infection is favorably improved.

The invention discloses a breeding method of a quaternary hybrid Sumei pig (a name of a hybrid pig) complete set line. The breeding method mainly comprises the following steps of breeding basic populations of Meishan pigs and Suhuai pigs with excellent reproductive performance and meat quality; breeding basic populations of Berkshire pigs and Pietrain pigs with excellent growth performance and slaughtering performance; hybridizing Meishan sows and Suhuai boars to cultivate Huai-Mei synthetic lines, and breeding Huai-Mei pigs with excellent reproductive performance and meat quality as parentalgeneration pigs of the complete set line; hybridizing Berkshire sows and Pietrain boars to cultivate a Pietrain-Berkshire synthetic line, and breeding Pietrain-Berkshire pigs with excellent growth performance and slaughtering performance as parental pigs of the complete set line; and hybridizing the parental Huai-Mei sows with the parental Pietrain-Berkshire boars to obtain the Sumei pig completeset commercial pig. The Sumei pig complete set line cultured by the method is black in whole coat hair, has the hybridization advantages of high reproductive performance and fresh and tender meat of domestic local pigs, also has the hybridization advantages of high growth performance and high lean meat percentage of foreign pigs, is tender and fragrant in meat, high in productivity and high in market competitiveness, and improves the pig raising production efficiency.