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Molecular marker related to skin color of chicken, primer group and application

A molecular marker and skin color technology, applied in the field of poultry genetics and breeding, can solve the problems of high cost, high molecular marker cost, less molecular marker research and mining, etc., and achieve the effect of low cost, high accuracy and fast speed.

Active Publication Date: 2021-06-04
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason is that, on the one hand, there are few molecular markers research and excavation on the economic traits of chickens, and the molecular markers that can be applied to select important economic traits in actual breeding are insufficient; on the other hand, the detection of molecular markers is often costly The cost of applying molecular marker-assisted selection technology in chicken breeding with short and large breeding population is higher than that of conventional breeding methods

Method used

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  • Molecular marker related to skin color of chicken, primer group and application
  • Molecular marker related to skin color of chicken, primer group and application
  • Molecular marker related to skin color of chicken, primer group and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening SNP sites

[0024] 1.1 Individuals used to screen SNP sites

[0025] The selected breeds and the quantity of each breed are as follows: 12 Guangxi Lingshan chickens, 12 bantam yellow chickens, 12 Jiangfeng Mahuang chickens, 10 Zhicheng Xinghua chickens, and 10 recessive white-feathered Locke chickens.

[0026] 1.2 Selection of candidate genes

[0027] The LPIN1 gene was selected.

[0028] 1.3 Genomic DNA extraction

[0029] Use the blood DNA extraction kit to extract chicken genomic DNA, operate according to the blood DNA extraction instructions, extract the genomic DNA of the blood sample, detect the DNA sample concentration and OD value, and make the DNA concentration greater than 25ng / μl, OD 260 / OD 280 Samples with a ratio between 1.7-1.8 were stored at -20°C for use.

[0030] 1.4 Candidate gene primers

[0031] According to the chicken candidate genome sequence published in NCBI, use the NCBI online primer design tool (https: / / www.ncbi.nlm...

Embodiment 2

[0039] Embodiment 2 time-of-flight mass spectrometry analysis

[0040] The SNP sites related to skin color obtained by direct sequencing were genotyped in 500 N409 (Guangxi Lingshan chicken) populations using the time-of-flight mass spectrometry typing technology of Beijing Liuhe Huada Gene Technology Co., Ltd., through SPSS 21.0 software Association analysis was performed on the combination of SNP typing results and color-related slaughter traits, and SNPs significantly associated with skin color were identified, as shown in the table below.

[0041] Table 2 SNP information table

[0042]

[0043]

[0044] Note: a means redness.

Embodiment 3

[0045] Example 3 Difference analysis between genotypes of SNPs significantly associated with skin color

[0046] Through SPSS 21.0 software, the analysis results of different genotypes of SNP loci significantly related to skin color and traits such as chest a, chest b, leg a and leg b are shown in Table 3 below.

[0047] Table 3 Difference analysis of SNP genotypes and skin color traits

[0048]

[0049] Note: a means redness, b means yellowness. The number in parentheses after the genotype indicates the number of samples of the genotype.

[0050] Therefore, LPIN1 gene marker-assisted selection can be used to select broiler chickens with suitable skin color without removing feathers in broiler chicken breeding, so as to promote the breeding work of high-quality chickens.

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Abstract

The invention discloses a molecular marker related to skin color of chicken, a primer group and application, and relates to the technical field of poultry genetic breeding. The invention provides an SNP related to the skin color of the chicken. The nucleotide sequence of the SNP is shown as SEQ ID NO: 1. The invention also provides a specific primer group for detecting the SNP and a specific detection method, which can detect the SNP sequence efficiently, sensitively and quickly so as to identify the skin color. The SNP site disclosed by the invention is unique, has very high broad-sense heritability, can accurately identify the color of the chests or legs of the broiler chicken after their molt, and improves the consistency of the skin color and the shin color of offspring chicken flocks of the broiler chicken.

Description

technical field [0001] The invention relates to the technical field of poultry genetics and breeding, in particular to molecular markers, primer sets and applications related to chicken skin color. Background technique [0002] The quality of poultry meat has a certain relationship with skin color, and its color is usually used as an appearance index of livestock and poultry products. my country's poultry meat consumption market is mainly dominated by white-feather and yellow-feather broilers, and yellow-feather broilers are preferred by consumers because of their good meat quality and flavor. With the improvement of living standards and the development of the industry, the production method of chicken has gradually shifted to large-scale breeding and centralized slaughtering, and the consumption direction of chicken has gradually changed to low-temperature meat products, cold fresh whole chicken, cold fresh cut chicken, frozen chicken Become a major chicken consumer produc...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02P60/87
Inventor 罗庆斌张细权聂庆华张德祥罗文李恒丰李红梅叶茂詹惠娜
Owner SOUTH CHINA AGRI UNIV
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