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Phalaenopsis amabilis tissue culture propagation method

A technique of phalaenopsis and tissue culture, applied in the field of phalaenopsis tissue culture and reproduction, can solve the problems of large damage to the mother plant, affecting the ornamental value, economic value and the like, and achieve the effect of improving the survival rate

Inactive Publication Date: 2016-11-09
成都东山兰韵农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many reports about the tissue culture of Phalaenopsis, and the leaves, stem tips, pedicel axillary buds, seeds, root tips, and pedicel internodes of Phalaenopsis are mostly used as explants for tissue culture research, and the study of using pedicels as explants Pedicels that have not bloomed and are about to bloom are often used, which will cause greater damage to the mother plant and will affect its ornamental value and economic value.

Method used

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  • Phalaenopsis amabilis tissue culture propagation method
  • Phalaenopsis amabilis tissue culture propagation method

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Induction of pedicel buds: inducing vegetative buds from the axillary buds at the 2nd and 3rd joints of the pedicel segment in the induction medium;

[0024] The induction medium is 3.0 mg of 6-BA, 1.0 g of Huabao No. 1, 30 mg of citric acid, 100 g of mashed potatoes, and 30 g of white sugar in each liter of MS basic medium;

[0025] Differentiation and proliferation of clustered buds: cut the vegetative buds from the base of the pedicel segment, and carry out the differentiation and proliferation of clustered buds in the differentiation medium, 25±1°C, light 1500Lx; the light time is 10 hours / day, culture until differentiation out of seedlings;

[0026] The differentiation medium is 5.0mg of 6-BA, 0.5mg of NAA, 10% coconut milk and 30mg of citric acid added to each liter of MS basic medium;

[0027] Rooting of strong seedlings: When the clustered buds grow to 1.5cm, they are cut off and transferred to the medium for strong seedlings to take root, and the seedlings are...

Embodiment 2

[0030] Induction of pedicel buds: inducing vegetative buds from the axillary buds at the 2nd and 3rd joints of the pedicel segment in the induction medium;

[0031] The induction medium is 3.0 mg of 6-BA, 1.0 g of Huabao No. 1, 30 mg of citric acid, 150 g of mashed potatoes, and 30 g of white sugar in each liter of MS basic medium;

[0032] Differentiation and proliferation of clustered buds: cut the vegetative buds from the base of the pedicel segment, and carry out the differentiation and proliferation of clustered buds in the differentiation medium, 25±1°C, light 2000Lx; the light time is 12 hours / day, culture until differentiation out of seedlings;

[0033] The differentiation medium is 5.0mg of 6-BA, 0.5mg of NAA, 10% coconut milk and 30mg of citric acid added to each liter of MS basic medium;

[0034] Rooting of strong seedlings: When the clustered buds grow to 1.5cm, they are cut off and transferred to the medium for strong seedlings to take root, and the seedlings are...

Embodiment 3

[0037] Induction of pedicel buds: inducing vegetative buds from the axillary buds at the 2nd and 3rd joints of the pedicel segment in the induction medium;

[0038] The induction medium was added with 3.0mg of 6-BA, 1.0g of Huabao No.1, 30mg of citric acid, 100g of banana puree, and 30g of white sugar per liter of VW basic medium;

[0039] Differentiation and proliferation of clustered buds: cut the vegetative buds from the base of the pedicel segment, and carry out the differentiation and proliferation of clustered buds in the differentiation medium, 25±1°C, light 1800Lx; the light time is 10 hours / day, culture until differentiation out of seedlings;

[0040] The differentiation medium is 5.0mg of 6-BA, 0.5mg of NAA, 10% coconut milk and 30mg of citric acid added to each liter of MS basic medium;

[0041] Rooting of strong seedlings: When the clustered buds grow to 1.5cm, they are cut off and transferred to the medium for strong seedlings to take root, and the seedlings are ...

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Abstract

The invention relates to the field of plant tissue culture, in particular to a phalaenopsis amabilis tissue culture propagation method. The phalaenopsis amabilis tissue culture propagation method comprises the following steps of induction of pedicel buds, differentiation and proliferation of cluster buds, shoot strengthening and root induction, seedling hardening and transplanting. An induction culture medium is prepared from mashed potatoes and mashed bananas, and saccharose is replaced by white granulated sugar, so that the production cost of large-scale proliferation of phalaenopsis amabilis can be greatly reduced, and the growth situation is good; coconut milk in a differentiation and proliferation culture medium is also one of key factors of proliferation, and the proliferation rate, the proliferation time and the economic benefit are the best when the content of the coconut milk is 10 percent. By adopting the phalaenopsis amabilis tissue culture propagation method of the technical scheme, tissue culture and rapid propagation are carried out along a path of the cluster buds of the phalaenopsis amabilis, the phalaenopsis amabilis tissue culture propagation method has the advantages of high inductivity, short induction time, high proliferation rate of the cluster buds, low technical difficulty and stable heritability, a large amount of variation can be prevented from happening during production, and a great significance in large-scale production is obtained.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a moth orchid tissue culture propagation method. Background technique [0002] Phalaenopsis amabilis (Phalaenopsis amabilis), also known as Phalaenopsis orchid, is known as the "Queen of Orchids" and is a treasure among tropical orchids. Phalaenopsis has huge flowers, many flowers, long flowering period, bright colors and rich colors. It can be potted and placed in the study, living room, bedroom, etc. It is elegant and generous and gives people beautiful enjoyment. The flowers can be used as bouquets and boutonnieres for brides , boutonniere, and a good material for cut flowers. Phalaenopsis is one of the most widely cultivated and popular varieties in the orchidaceae, and is loved by people all over the world. Phalaenopsis is a single-stem aerial orchid. The plants rarely develop side branches, and the seeds are extremely difficult to germinate. The seedlings are highly va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 宋林贵苟兴明
Owner 成都东山兰韵农业有限公司
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