41 results about "Phalaenopsis amabilis" patented technology

The invention discloses a method for cultivating moth orchid in a solar greenhouse in the north, and belongs to a flower and plant cultivation method. A method for constructing the solar greenhouse comprises the steps of mounting round-wing heating pipes around the greenhouse, building a seedling bed, mounting a second curtain for the seeding bed in winter and covering a shading net outside the greenhouse in spring, summer and autumn. The method for cultivating moth orchid comprises the steps of selecting seeds, cultivating seedlings in test tubes, changing pots, watering and applying fertilizer, spraying agricultural chemical, arranging seedlings and seedling plates, cultivation temperature, light control standards, water and humidity control standards, fertilizer use standards N-P-N, flowering period regulation, supporting stalks, sorting finished flowers and packing and transporting finished flowers. The method cultivates moth orchid with exuberant branches and leaves and buds through improvement of the solar greenhouse, regulation of cultivation environment and flowering period, control of diseases and insects and finds a new field for cultivating moth orchid in the north.

The invention provides a culture medium for phalaenopsis amabilis tissue culture. The culture medium comprises the following components: 1900 mg / L of KNO3; 1650 mg / L of ammonium nitrate; 170 mg / L of potassium dihydrogen phosphate; 370 mg / L of magnesium sulfate heptahydrate; 440 mg / L of calcium chloride dihydrate; 0.60-0.62 mg / L of KI; 4.5-4.8 mg / L of boric acid; 25.0-25.3 mg / L of manganese sulfate tetrahydrate; 7.0-7.2 mg / L of zinc sulfate heptahydrate; 0.20-0.22 mg / L of sodium molybdate dihydrate; 0.025 mg / L of copper sulfate pentahydrate; 0.025 mg / L of cobalt chloride hexahydrate; 37.25 mg / L of Na2.EDTA; 27.85 mg / L of ferrous sulfate heptahydrate; 100 mg / L of inositol; 2 mg / L of glycine; 0.1 mg / L of thiamine hydrochloride; 0.5 mg / L of pyridoxine hydrochloride; 0.5 mg / L of nicotinic acid; 20-22 g / L of sucrose; 5-5.2 g / L of agar; 30-35 g / L of a banana juice; 3-5 g / L of a pear juice; 0.45-0.48 g / L of activated carbon; and a solvent being deionized water. With application of the culture medium to culture phalaenopsis amabilis, the survival rate is more than 96%, the bacteria infection rate is 2.7-2.8%, and the browning degree of the culture medium is 30-40% lower than that of an MS culture medium.

The invention relates to a combined method for preventing Phalaenopsis amabilis from browning in plant tissue culture, the adopted V31 (Phalaenopsis amabilis) is also called 'TaiLinhongtianshi', and is one of a red flower line of the Phalaenopsis amabilis. A culture medium containing natural additives is utilized for preventing the common browning phenomenon of the Phalaenopsis amabilis, so as to form the combined browning-prevention method. The method has high stability and high success rate, is green and environment-friendly, can be repeatedly used for production annually, and solves the problem that the existing method has low success rate and is not environment-friendly, thereby having great significance of real-world applications.

The invention discloses a tissue culture method for phalaenopsis amabilis; in the steps, the inoculation density and the sowing density can produce a 'quorum sensing', so that the seed germination becomes faster; a sterile germination culture medium includes tryptone, a banana juice, active carbon and agar and the ratio and can provide nutrition needed for seed growth, and the germination of the seeds is promoted; hormones are added during germination, the adult seedling number can be allowed to be increased; an organic additive is added during germination, the organic additive is saccharose, the saccharose concentration is 2-4%, and the organic additive has a promoting effect on phalaenopsis amabilis seed germination, protocorm formation and leaf growth. The phalaenopsis amabilis cultured by the tissue culture method has the advantages of high germination rate, excellent growth, huge market potential and broad prospects.

The invention relates to the field of plant tissue culture, in particular to a phalaenopsis amabilis tissue culture propagation method. The phalaenopsis amabilis tissue culture propagation method comprises the following steps of induction of pedicel buds, differentiation and proliferation of cluster buds, shoot strengthening and root induction, seedling hardening and transplanting. An induction culture medium is prepared from mashed potatoes and mashed bananas, and saccharose is replaced by white granulated sugar, so that the production cost of large-scale proliferation of phalaenopsis amabilis can be greatly reduced, and the growth situation is good; coconut milk in a differentiation and proliferation culture medium is also one of key factors of proliferation, and the proliferation rate, the proliferation time and the economic benefit are the best when the content of the coconut milk is 10 percent. By adopting the phalaenopsis amabilis tissue culture propagation method of the technical scheme, tissue culture and rapid propagation are carried out along a path of the cluster buds of the phalaenopsis amabilis, the phalaenopsis amabilis tissue culture propagation method has the advantages of high inductivity, short induction time, high proliferation rate of the cluster buds, low technical difficulty and stable heritability, a large amount of variation can be prevented from happening during production, and a great significance in large-scale production is obtained.

The invention relates to a special soilless culture nutritional meal for butterfly orchid and preparation and application of the special soilless culture nutritional meal. The special soilless culture nutritional meal for the butterfly orchid consists of a nutritional matrix and a nutritional fertilizer in mass ratio of (970-985):(15-30), wherein the nutritional fertilizer consists of a nutritional fertilizer A, a nutritional fertilizer B and a nutritional fertilizer C in mass ratio of (1-2.5):(4.5-6):(2.5-3.5). The nutritional matrix comprises expanded perlite, expanded vermiculite, sawdust,calcium chloride, magnesium chloride and ferrous sulfate in raw material. The nutritional matrix, the nutritional fertilizer A, the nutritional fertilizer B and the nutritional fertilizer C are respectively and independently packaged. According to the special soilless culture nutritional meal for the butterfly orchid, the nutritional matrix compounded in consideration of absorption of nourishmentat different growth stages of the butterfly orchid is suitable for the well growth of the butterfly orchid in water-retaining property, nutrient preserving capability and vent ability, and meanwhile,the nutritional fertilizer is blended into an aqueous solution applied in the manner of watering, and through tests, a good effect is obtained.

The present invention relates to a method for cultivating phalaenopsis amabilis in a northern glasshouse. The method comprises the following steps: planting phalaenopsis amabilis seedlings in a matrix (a mixture of peat soil and perlite according to a volume ratio of 4:1 to 5:1); thoroughly watering the whole matrix with water having a pH value of 6.0-6.8; spraying the plant with a 800-time liquid of chlorothalonil wettable powder 2-4 days after watering; transferring the mature phalaenopsis amabilis seedlings into a greenhouse 4-5 months before expected flowering; keeping temperature of the greenhouse at 30-32 DEG C during daytime and at 24-26 DEG C during night; adjusting illumination in the greenhouse to 10000-12000 Lux; and applying fertilizers at regular intervals, wherein a balance fertilizer with a N:P:K ratio of 20:20:20 is applied every week along with water.

The invention provides an optimized manufacturing method of paraffin sections of different flower tissues of phalaenopsis amabilis. The manufacturing method comprises the following steps: (1) fixing;(2) dehydrating; (3) waxing; (4) embedding; (5) slicing and unfolding; (6) dewaxing and rehydrating; (7) toluidine blue dyeing and microscopic examination, and carrying out proper differentiation anddrying according to tissue coloring depth; and (8) performing transparent sealing. Components and operation time of reagents in fixing, softening and dewatering links are optimized for different flower tissues of the phalaenopsis amabilis, the completeness of cells in slices is better kept, and unlignified soft tissues and lignified hard tissues can be clearly distinguished.

The invention provides a method for quickly breeding a new butterfly orchid variety. The method sequentially comprises the following two steps: cross pollination, sterile seeding, seed germination anddifferentiation culture. The differentiation stage is divided into two stages: (1) picking out 5-10 rootless seedlings, respectively and independently carrying out multiplication subculture, and carrying out rooting and transplantation culture to obtain meristematic seedlings; and (2) carrying out rooting culture on the remaining seedlings to obtain seedling plants. The meristematic seedlings andthe seedling plants are respectively subjected to seedling exercise, are taken out of a bottle, are transplanted, and finally forms flowering plants though planting small, medium and large seedlings.The method screens out high-quality strains through comparison, forms a new variety through testing and evaluation, screens out high-quality individuals which do not exist in the meristematic seedling flowering plants from the flowering plants of the seedling plants, and forms the new variety through single-plant cloning propagation, planting, testing and evaluation. According to the method, thenew variety breeding time is obviously shortened, the phalaenopsis amabilis breeding efficiency is remarkably improved, and complementary advantages are formed by combining a rapid breeding method anda traditional breeding method.

The invention relates to the field of cultivation of phalaenopsis amabilis, and particularly discloses a water-cooling flower forcing method for phalaenopsis amabilis. The phalaenopsis aphrodite is placed in a greenhouse to be cultivated, and flower forcing operation is carried out on the phalaenopsis aphrodite from plantlets of the phalaenopsis Aphrodite. The flower forcing operation comprises the following steps that 1, water curtain walls are installed on the two sides of the greenhouse, water vapor in the water curtain wall on one side of the greenhouse is blown into the greenhouse through a draught fan in the daytime every day, and the temperature in the greenhouse is controlled to range from 22 DEG C to 23 DEG C; and 2, a spraying device and a spray system are installed in the greenhouse, and water mist with the temperature ranging from 13 DEG C to 15 DEG C is sprayed into the greenhouse through the spraying device at dusk every day, and meanwhile, water drops with the temperature of 13 DEG C-15 DEG C are sprayed into the culture pot through the spray system. The cultivation period of the phalaenopsis amabilis is shortened, and the cultivation cost of the phalaenopsis amabilis is greatly reduced.

The invention discloses a preparation method of a proliferation medium for raising phalaenopsis amabilis seedlings and belongs to a culture medium for raising flower and plant seedlings. The proliferation medium for raising the phalaenopsis amabilis seedlings mainly comprises the following components: a main material (mother liquid), auxiliary materials, an additive and a hormone which are scientifically prepared to from a soilless culture solution for raising the phalaenopsis amabilis seedlings. Fruits of plants are used as the main raw materials. In addition, most of natural vegetable matters are adopted in the formula, vitamins contained in the original basal culture medium can be improved by the content of proportioned vegetable matters, the proliferation medium not only contains main nutrients, but also contains a proper quantity of trace elements, and the nutrients and the trace elements can be used for promoting the differentiation of callus of phalaenopsis amabilis and the elongation of cells, preventing tissues from aging, keeping plants robust and bright green, playing a favorable effect on the seedling culture, the propagation, the intermediate transplantation and the like of the phalaenopsis amabilis and promoting the growth of substances, so that an expected effect can be achieved.

The invention provides a gene PeGRF6 for regulating and controlling development of butterfly orchid leaves and application of the gene PeGRF6. The invention firstly provides a gene PeGRF6 for regulating and controlling the development of butterfly orchid leaves. The gene PeGRF6 has one of the following nucleotide sequences: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a nucleotide sequence which is derived by substitution, deletion or addition of one or more nucleotides of the nucleotide sequence as shown in SEQ ID NO.1; and 3) a nucleotide sequence having at least 80% homology with the SEQ ID NO.1. The gene provided by the invention can effectively regulate and control the development of the leaves of the Phalaenopsis amabilis, reduce the leaf area of new leaves of the Phalaenopsis amabilis, improve the content of anthocyanin and chlorophyll in the leaves, and provide a theoretical basis for breeding of the Phalaenopsis amabilis.

The invention discloses a method for preventing and controlling phalaenopsis amabilis brown spot. The method is characterized by comprising the following steps of: mixing turfy soil and perlite according to a volume ratio of 4:1-5:1 to form a culture substrate, wherein the temperature is between 25 and 28 DEG C in the daytime and is between 18 and 20 DEG C in the evening, the humidity is between 70 and 80 percent in the daytime and is between 75 and 85 percent in the evening, and the illumination is between 10,000 and 12,000Lux; spraying solution formed by dissolving thiophanate methyl wettable powder in 800 to 1,000 times water once every month after seedlings are introduced; continuously spraying for 5 months; and irrigating with water or fertilizer in the following way during the spraying: irrigating with clear water when emission concentration (EC) value of soil is more than 0.4; and irrigating with aqueous solution which is formed by mixing 0.3 to 0.4g of peters product 20-10-200, 0.05 to 0.07g of peters product 15-20-25 and 0.16 to 0.18g of MgSO4 in every liter of water and aqueous solution which is formed by dissolving 0.8 to 0.9g of peters product 20-20-20 in every liter of water in turn when the EC value of the soil is less than 0.4.

The invention belongs to the technical field of monitoring of plant varieties and in particular discloses a method for detecting phalaenopsis amabilis mutants. Four varieties including 'HongMantianHong', 'Guangmangsishe', V31 and 'Hongzhenzhu' and mutants thereof can be completely distinguished through utilizing combination of ten pairs of primers including SSR1, SSR2, SSR3, SSR4, SSR5, SSR6, SSR7, SSR8, SSR9 and SSR10; sequences of the ten pairs of primers including the SSR1, the R2, the SSR3, the SSR4, the SSR5, the SSR6, the SSR7, the SSR8, the SSR9 and the SSR10 are shown as SEQ: 1 to 20. The detection method disclosed by the invention can be used for screening the mutants, which do not meet growth requirements, at a bottle seedling period, so that the production of large-scale production is saved.

The invention provides a method for inducing butterfly orchid to bloom, which mainly researches the secondary blooming characteristic of butterfly orchid, and promotes the blooming of butterfly orchid and shortens the secondary blooming time of butterfly orchid in combination with the use of a plant growth regulator and an environment management method. According to the technical scheme, the method is mainly used for evenly spraying an exogenous growth regulator on leaf surfaces, the exogenous growth regulator is a solution of gibberellin or cytokinin, methyl jasmonate and indole-3-acetic acid, and meanwhile environment management is matched, so that the purpose of shortening the flowering time of phalaenopsis amabilis is achieved.

The utility model relates to the technical field of phalaenopsis planting, and discloses a seedbed translation unit for phalaenopsis planting, which comprises a first seedbed transportation rail, a second seedbed transportation rail and a plurality of seedbed placing frames positioned between the first seedbed transportation rail and the second seedbed transportation rail, a first lifting assembly is arranged on the inner side of the first seedbed conveying rail, and a horizontal moving transverse frame is arranged at the top end of the seedbed containing frame. According to the seedbed translation unit for phalaenopsis planting, through the design of the transverse frame, the first lifting assembly, the second lifting assembly, the auxiliary horizontal pushing assembly and the bed pushing assembly, translation operation of the seedbed assembly can be achieved, and the auxiliary horizontal pushing assembly can assist the seedbed assembly in further translation so that the seedbed assembly can enter the upper portion of the second seedbed conveying rail; the seedbed assembly can be transversely transported in cooperation with the seedbed driving unit and the seedbed transporting unit, logistics circulation among a plurality of greenhouses can be achieved, the logistics efficiency is improved, and meanwhile the planting efficiency is greatly improved.

The utility model discloses a butterfly orchid flower forcing greenhouse, and relates to the technical field of greenhouses, the butterfly orchid flower forcing greenhouse comprises an alloy skeleton, a plurality of light-transmitting plates are arranged on the alloy skeleton, an information plate is arranged on the surface of one of the light-transmitting plates, a greenhouse door is hinged to the alloy skeleton, and the information plate is arranged on the surface of the other light-transmitting plate. The greenhouse comprises an alloy framework, shielding mechanisms used for controlling air circulation in the greenhouse and blocking pests are arranged on the alloy framework, cleaning mechanisms used for cleaning dust in a reciprocating mode are arranged on the shielding mechanisms, the number of the shielding mechanisms and the number of the cleaning mechanisms are both two, and protective shells are arranged on the two shielding mechanisms and the two cleaning mechanisms. According to the utility model, the humidity in the greenhouse is controlled, the air composition in the greenhouse is adjusted, harmful gas is exhausted, the content of carbon dioxide in the air is improved, the phalaenopsis has a good growth environment, pests can be prevented from entering the greenhouse, the planting environment is improved, the operation is simple, and the practicability is strong.

The invention discloses a method for determining the opportunity of inducing 2n pollen of phalaenopsis amabilis by colchicine. The method comprises the following steps of (a) counting the yield of natural 2n pollen; (b) conducting 2n pollen induction: selecting the pollen in each meiosis period, and treating flower buds with 0.01%-0.1% colchicine solution to perform 2n pollen induction; (c) counting the 2n pollen rate after the induction; and (d) comparing the 2n pollen rate obtained after induction with the natural 2n pollen rate so as to determine the opportunity for inducing the 2n pollen. The invention also provides a method for inducing 2n pollen of phalaenopsis amabilis by using the colchicine. According to the method, the opportunity for inducing the 2n pollen by utilizing the colchicine and the corresponding colchicine concentration are determined, and a foundation is laid for obtaining the 2n pollen through polyploid induction and cultivating a new phalaenopsis variety which is large in flower size, bright in color and high in ornamental value.

The invention discloses a pedicel bud cloning culture medium formula of phalaenopsis amabilis. The culture medium formula comprises an MS culture medium, 20-40 g / L of sugar, 120-150 ml / L of coconut juice, 5-12 mg / L of NAA, 20-25 mg / L of 6Ba, 4-6 g / L of citric acid and 5-10 g / L of agar. The invention further discloses a culture medium filling device. On the basis of an MS culture medium, substancessuch as coconut juice, NAA and 6Ba are added, the propagation coefficient in the rapid propagation process of phalaenopsis amabilis can be increased, grown plantlets are promoted to be neat and robust, citric acid is added to serve as a polyphenol oxidase inhibitor, and the technical problems that commercial plantlets produced through an existing method are low in yield and inconsistent in flowering phase are solved; the culture medium filling device can stably convey a culture medium to the discharging opening through the material conveying screw, conical flasks are placed in the limiting clamping grooves, rotary filling is achieved, on one hand, the filling efficiency is improved, and on the other hand, stable conveying of the culture medium can be guaranteed.

The invention discloses a tissue culture medium for phalaenopsis. The culture medium comprises an induction culture medium, a proliferation culture medium, a seedling strengthening culture medium and a rooting culture medium, the invention relates to a tissue culture propagation method of phalaenopsis amabilis, which comprises the following steps: 1) material disinfection, 2) explant inoculation, 3) propagation culture of tissue culture seedlings, 4) strong seedling culture, 5) rooting culture, 6) acclimatization and domestication, and 7) transplanting. The method has the advantages that the multiplication culture period is short, the propagation coefficient is high, the transplanting survival rate can reach 99% or above, and the method can efficiently and rapidly provide high-quality phalaenopsis seedlings. By the adoption of the method, a large number of seedlings capable of being planted out of bottles can be obtained, the method is not affected by factors such as seasons, high-quality phalaenopsis seedlings can be efficiently and rapidly provided, and the good variety popularization speed can be increased.

The utility model discloses a Phalaenopsis aphrodite flower forcing greenhouse, which relates to the technical field of greenhouses, and comprises a greenhouse main body and two sunshade nets, the greenhouse main body is provided with a moving mechanism for transverse movement, the moving mechanism is provided with a rolling mechanism for rolling and unrolling the sunshade nets, the sunshade nets are arranged on the rolling mechanism, and the sunshade nets are arranged on the rolling mechanism. The greenhouse comprises a greenhouse body, two ventilation openings are formed in the greenhouse body in a penetrating mode, fixing frames are arranged in the two ventilation openings, insect-proof nets are arranged on the two fixing frames, two sets of adjusting mechanisms used for shielding and opening the ventilation openings are arranged on the two fixing frames, and the adjusting mechanisms are arranged on the greenhouse body. The moving mechanism comprises two one-way lead screws, the two one-way lead screws are rotationally installed on the greenhouse body, and moving rods are installed on the outer walls of the two one-way lead screws in a threaded mode, so that the sunshade net can be conveniently unfolded and rolled, the ventilation opening can be conveniently shielded and opened, operation is easy, and practicability is high.

The invention discloses a method for cultivating phalaenopsis amabilis with good growth vigor, and relates to the technical field of plant cultivation. According to the method, raw materials of a culture medium are fiber raw materials such as bark, tree fern roots and coconut shells; the smashed fiber raw materials such as the bark, the tree fern roots and the coconut shells are evenly mixed withfine broken stone blocks according to the volume ratio of 1:1 to form base fertilizer, and the base fertilizer not only contains a nutrient medium for growth and development of the phalaenopsis amabilis, but also is loose and ventilated, so that the root periphery of the planted phalaenopsis amabilis has enough oxygen content, and aerial roots of the phalaenopsis amabilis can absorb oxygen and nutrition from the surroundings more easily; and a flowerpot is formed by weaving bamboo canes, the flowerpot formed by weaving the bamboo canes has air permeability, the base fertilizer in the flowerpotcan exchange air with the outside atmosphere, air exchange between the air in the base fertilizer and the outside atmosphere is increased, oxygen in the base fertilizer is replaced, and growth and development of the aerial roots of the phalaenopsis amabilis are facilitated.

The invention discloses a soil conditioner for planting golden camellia. The soil conditioner is prepared from the following raw materials: rice straw powder, zoysia japonica, broom grass, phalaenopsis amabilis, a complex microbial inoculant, sodium humate, triptolide, mono-ammonium phosphate, potassium sulfate, compound sodium nitro-phenolate, diatomite, hydrochloric acid, fatty alcohol-polyoxyethylene ether ammonium sulfate, sodium carbonate and croscarmellose sodium through the steps of mixing, modifying, granulating, drying and the like. After soil improvement, the soil conditioner is suitable for planting teaks and golden camellia; the teak is high in adaptability to soil and can well grow in acid soil, the golden camellia and the teak like to grow in a wet environment, thus the golden camellia is suitable for being planted in a teak forest; the output of golden camellia is high, and for each mu, the output of golden camellia can reach 47.23 kg or more in the fourth year.

The invention discloses a method for preventing and controlling phalaenopsis amabilis brown spot. The method is characterized by comprising the following steps of: mixing turfy soil and perlite according to a volume ratio of 4:1-5:1 to form a culture substrate, wherein the temperature is between 25 and 28 DEG C in the daytime and is between 18 and 20 DEG C in the evening, the humidity is between 70 and 80 percent in the daytime and is between 75 and 85 percent in the evening, and the illumination is between 10,000 and 12,000Lux; spraying solution formed by dissolving thiophanate methyl wettable powder in 800 to 1,000 times water once every month after seedlings are introduced; continuously spraying for 5 months; and irrigating with water or fertilizer in the following way during the spraying: irrigating with clear water when emission concentration (EC) value of soil is more than 0.4; and irrigating with aqueous solution which is formed by mixing 0.3 to 0.4g of peters product 20-10-200, 0.05 to 0.07g of peters product 15-20-25 and 0.16 to 0.18g of MgSO4 in every liter of water and aqueous solution which is formed by dissolving 0.8 to 0.9g of peters product 20-20-20 in every liter of water in turn when the EC value of the soil is less than 0.4.

The utility model discloses a butterfly orchid combined potting flowerpot. The flowerpot comprises a placing shell; the flowerpot fixing plate is assembled on the placing shell and is used for fixing a flowerpot; the breathable flowerpot device is assembled in the flowerpot fixing plate and is used for planting orchid; according to the device, the auxiliary dehumidification device is mounted on one side of the placement shell, and the dehumidification plate is detachably mounted on the inner side through the mounting groove. Then, a ventilation and dehumidification device is arranged, the ventilation and dehumidification device comprises a water guide slope fixed in the containing shell, a fan is fixed to one side of the containing shell, a water outlet is formed in the other side of the containing shell, and meanwhile a water storage tank is fixed to the other side of the containing shell; therefore, when the fan works, air flow can be guided to discharge moisture through the water outlet, meanwhile, the water storage tank collects water which may overflow, and the water guide slope helps water flow to smoothly flow to the water outlet. The whole system works cooperatively and provides appropriate humidity and ventilation conditions for phalaenopsis amabilis.

The invention discloses a composition with anti-wrinkle and firming effects. The composition is prepared from the following components: a hemerocallis fulva flower extract, a lilium davidii linn / leaf extract, a fructus chebulae bark extract, a magnolia liliiflora bud extract, a phalaenopsis aphrodite extract, butanediol, hexanediol and water. The composition is safe, stable, efficient and mild, formed wrinkles and relaxation problems can be remarkably improved, new wrinkles can be prevented, through the synergistic effect of plant components, the irritation is greatly reduced while the effect is guaranteed, and sensitive skin can also safely use efficient anti-aging products.

The invention discloses a phalaenopsis amabilis summer forcing culture method. The method is characterized by mainly including the following steps: step1, transplanting large mature phalaenopsis amabilis seedlings into a greenhouse 4-5 mouths before expected blooming, keeping the greenhouse at the temperature of 24-32 DEG C in the daytime and 18-26 DEG C at night, and carrying out fertilization regularly, step2, keeping the greenhouse at the temperature of 24-26 DEG C in the daytime and 18-20 DEG C at night after January, carrying out fertilization regularly, and spraying 3-7ppm of multi-effect triazole (pp333) on leaves 2-4 times, and step3, keeping the greenhouse at the temperature of 25-28 DEG C in the daytime and 20-22 DEG C at night 2-3 mouths before expected blooming, and carrying out fertilization regularly till phalaenopsis amabilis blooms. The phalaenopsis amabilis summer forcing culture method has the advantages that summer flower forcing peduncle growth rates are high, the number of flowers of a single plant after the phalaenopsis amabilis blooms is large, flower color is bright, summer flower forcing cost is low, and a large amount of phalaenopsis amabilis can bloom and appear on the market in summer and autumn in the North China.

The invention discloses a rapid propagation method of phalaenopsis amabilis flower stalk stem sections. The method comprises the following steps of (1) preparing a culture medium; (2) preparing explants; (3) performing inoculation; and (4) performing management after inoculation. The rapid propagation method of the phalaenopsis amabilis flower stalk stem sections provided by the invention has theadvantages that the browning and deterioration of callus tissues are effectively prevented; the bacterial infection rate of the tissue culture of phalaenopsis amabilis is also greatly reduced; the callus tissue division, differentiation and proliferation capacity is improved; the propagation rate of the tissue culture of the phalaenopsis amabilis is further improved; and a high market popularization value is realized.

The invention discloses a method for preparing a rapid reproduction culture medium for tissue culture of phalaenopsis amabilis. The method comprises the following steps: (1) performing fumigation treatment; (2) performing crushing treatment; (3) performing homogenization treatment; (4) weighing raw materials; and (5) preparing a finished product. By adopting the culture medium finally prepared bythe method, rich nutrient elements can be provided for splitting and differentiation of phalaenopsis amabilis tissue, callus browning deterioration can be effectively prevented, the contamination rateof phalaenopsis amabilis tissue culture can be also greatly reduced, the callus splitting and differentiation capability can be improved, furthermore, the breeding rate of phalaenopsis amabilis tissue culture can be increased, and very high market popularization value can be made.

The invention discloses and provides a method for identifying soft rot resistance of phalaenopsis amabilis. The method comprises the steps: scissoring middle-lower leaves of a phalaenopsis amabilis variety, wiping leaves and leaf stalk wounds with alcohol once, and carrying out natural air-drying; inoculating a NA culture solution with pathogenetic strains, and preparing a bacterial suspension byusing sterile water; densely and gently puncturing centers of the leaves with sterile pins, dripping the bacterial suspension to punctured positions, placing the leaves in magnetic discs for moisturized sealing and dark culture; observing and recording onset conditions of the leaves when water-spot-shaped lesions emerge from the leaves; and carrying out grading by average control efficiency, and evaluating infection resisting characteristics of the variety.