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2 results about "Tree fern" patented technology
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The tree ferns are the ferns that grow with a trunk elevating the fronds above ground level. Most tree ferns are members of the "core tree ferns", belonging to the families Dicksoniaceae, Metaxyaceae, and Cibotiaceae in the order Cyatheales. This order is the third group of ferns known to have given rise to tree-like forms. The two others are the Marattiales, a eusporangiate order that the extinct Psaronius evolved from, and the order Polypodiales where the extinct genus Tempskya belongs.
Aspidium adiantum aspiMYB5 gene and application in regulating aspidium adiantum phenol biosynthesis
PendingCN122256377AFermentationVector-based foreign material introductionBiotechnologyPromoter activity
This invention discloses tree fern. AspiMYB5 The application of genes in regulating the biosynthesis of tree fern phenols belongs to the field of genetic engineering technology, which involves regulating the biosynthesis of tree fern phenols within plants. AspiMYB5 The expression level of the gene, thereby regulating the key enzyme gene of tree fern phenol. AspiPKS6 The expression of [a specific enzyme] and the synthesis of tree fern phenol. This invention uses a yeast library screening method to screen genes affecting key enzymes. AspiPKS6 Expressed transcription factors AspiMYB5 The results were verified using yeast single-hybrid technology (Y1H) and electrophoretic mobility variation analysis (EMSA). AspiMYB5 and AspiPKS6 The promoter exhibits direct physical binding in vitro. Dual-luciferase reporter gene assays further demonstrate that... AspiMYB5 and AspiPKS6 When the promoter is co-expressed, the fluorescence signal intensity is significantly reduced, further confirming... AspiMYB5 It can inhibit AspiPKS6 AspiMYB5 Promoter activity. This invention not only improves the theoretical research on the biosynthetic pathway of tree fern phenols, but also provides key target genes and technical support for regulating the content of secondary metabolites in rare plants through genetic improvement.
Owner:SICHUAN AGRI UNIV +2
A method for preserving and commercialized rapid propagation of green flowered tree fern germplasm
The present application relates to the technical field of plant tissue culture, and discloses a kind of green flowered rhododendron spathulatum germplasm preservation and commercialized rapid propagation method, comprising the following steps: by step S1: with mature leaf as explant, and embryogenic callus is induced to obtain;Induced embryogenic callus proliferation coefficient reaches 7.50;Step S2: after cutting the callus-embryo mixture obtained in step S1, it is inoculated in callus-embryo mixture proliferation medium and is proliferated to obtain cluster bud, and proliferation coefficient reaches 11.65;Step S3: after being divided into single plant, it is connected into blank MS medium and is transition cultured;Through separation cluster bud single culture, seedling coefficient is promoted from 3.1 to 12.5.Step S4: acclimatization transplanting is carried out.The total proliferation coefficient can reach more than 70.0 using the rapid propagation method of the application, and the survival rate reaches 100% after acclimatization transplanting, with stable ornamental traits.
Owner:YUNNAN FENGHE SHULI BIOTECHNOLOGY CO LTD