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Tissue culture method for phalaenopsis amabilis

A technique of tissue culture and Phalaenopsis, which is applied in the field of tissue culture of Phalaenopsis, can solve the problems of slow germination and low number of seedlings, and achieve the effects of faster germination, increased number of seedlings, and leaf growth promotion

Inactive Publication Date: 2016-11-16
ANHUI MEILAN LANDSCAPE ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current tissue culture method of Phalaenopsis has the problems of slow germination and low number of seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for tissue culture of Phalaenopsis, comprising the steps of:

[0026] (1) Seed collection

[0027] First select the Phalaenopsis fruit pods 95 days after pollination, the selected fruit pods are smooth and full outside, and the color turns from green to yellow;

[0028] (2) Inoculation

[0029] First scrub the surface of the capsule with a diluted detergent solution, then rinse it, then soak it in 8% NaClO for 10 minutes, then disinfect it with 75% alcohol for 1 minute on the ultra-clean workbench, and disinfect it with 0.05% mercury liter for 3 minutes, then wash it with sterile water Rinse twice, take out the capsules and put them in a sterile petri dish, cut the capsules and move the seeds into a culture bottle with sterile water, shake gently to disperse them evenly, and the inoculation density is 200 grains / m 2 ;

[0030] (3) germination

[0031] Seeds are germinated using seed sterile germination medium. After 10 days of inoculation, the embryos germi...

Embodiment 2

[0039] A method for tissue culture of Phalaenopsis, comprising the steps of:

[0040] (1) Seed collection

[0041] First select the Phalaenopsis pods 100 days after pollination, the selected pods are smooth and full outside, and the color turns from green to yellow;

[0042] (2) Inoculation

[0043] First scrub the surface of the capsule with a diluted detergent solution, then rinse it, then soak it in 10% NaClO for 15 minutes, then disinfect it with 75% alcohol for 2 minutes on the ultra-clean workbench, and disinfect it with 0.10% mercury liter for 3 minutes. Rinse 3 times, take out the capsules and put them in a sterile petri dish, cut the capsules and move the seeds into a culture bottle with sterile water, shake gently to disperse them evenly, and the inoculation density is 250 grains / m 2 ;

[0044] (3) germination

[0045] Seeds are germinated using seed sterile germination medium. After 15 days of inoculation, the embryos germinate, and after 20 days, they are trans...

Embodiment 3

[0053] A method for tissue culture of Phalaenopsis, comprising the steps of:

[0054] (1) Seed collection

[0055] First select the Phalaenopsis pods 105 days after pollination, the selected pods are smooth and full outside, and the color turns from green to yellow;

[0056] (2) Inoculation

[0057] First scrub the surface of the capsule with a diluted detergent solution, then rinse it, then soak it in 12% NaClO for 20 minutes, then disinfect it with 75% alcohol for 3 minutes on the ultra-clean workbench, and disinfect it with 0.15% mercury liter for 3 minutes. Rinse 4 times, take out the capsule and put it in a sterile petri dish, cut the capsule and move the seeds into a culture bottle with sterile water, shake gently to disperse them evenly, and the inoculation density is 300 grains / m 2 ;

[0058] (3) germination

[0059] Seeds are germinated using seed sterile germination medium. After 1020 days of inoculation, the embryos germinate, and after 20 days, they are transfe...

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PUM

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Abstract

The invention discloses a tissue culture method for phalaenopsis amabilis; in the steps, the inoculation density and the sowing density can produce a 'quorum sensing', so that the seed germination becomes faster; a sterile germination culture medium includes tryptone, a banana juice, active carbon and agar and the ratio and can provide nutrition needed for seed growth, and the germination of the seeds is promoted; hormones are added during germination, the adult seedling number can be allowed to be increased; an organic additive is added during germination, the organic additive is saccharose, the saccharose concentration is 2-4%, and the organic additive has a promoting effect on phalaenopsis amabilis seed germination, protocorm formation and leaf growth. The phalaenopsis amabilis cultured by the tissue culture method has the advantages of high germination rate, excellent growth, huge market potential and broad prospects.

Description

technical field [0001] The invention belongs to the field of flower cultivation, and more specifically, the invention relates to a tissue culture method of Phalaenopsis. Background technique [0002] Phalaenopsis, orchidaceae Phalaenopsis, is a single-stem tropical epiphytic orchid, distributed in tropical and subtropical regions of Asia and Oceania, and mostly grows on tree trunks and wet stones in damp, foggy and high-temperature forests. Showy, with a long flowering period, it has become one of the most popular flowers in the international and domestic flower markets. It is known as the "Queen of Orchids". Through sterile seeding and tissue culture, a large number of excellent plants can be obtained, which can maintain excellent quality characteristics, and is the key to large-scale commercial production and new variety breeding. The current tissue culture method of Phalaenopsis has the problems of slow germination and low seedling quantity. Contents of the invention ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 周克斌
Owner ANHUI MEILAN LANDSCAPE ENG CO LTD
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