Pedicel bud cloning culture medium formula of phalaenopsis amabilis

Abstract

The invention discloses a pedicel bud cloning culture medium formula of phalaenopsis amabilis. The culture medium formula comprises an MS culture medium, 20-40 g / L of sugar, 120-150 ml / L of coconut juice, 5-12 mg / L of NAA, 20-25 mg / L of 6Ba, 4-6 g / L of citric acid and 5-10 g / L of agar. The invention further discloses a culture medium filling device. On the basis of an MS culture medium, substancessuch as coconut juice, NAA and 6Ba are added, the propagation coefficient in the rapid propagation process of phalaenopsis amabilis can be increased, grown plantlets are promoted to be neat and robust, citric acid is added to serve as a polyphenol oxidase inhibitor, and the technical problems that commercial plantlets produced through an existing method are low in yield and inconsistent in flowering phase are solved; the culture medium filling device can stably convey a culture medium to the discharging opening through the material conveying screw, conical flasks are placed in the limiting clamping grooves, rotary filling is achieved, on one hand, the filling efficiency is improved, and on the other hand, stable conveying of the culture medium can be guaranteed.

Description

technical field

[0001] The invention belongs to the technical field of phalaenopsis cultivation, in particular to a formula of a phalaenopsis pedicel bud cloning medium. Background technique

[0002] Phalaenopsis belongs to the Orchidaceae plant and has the reputation of "Queen of Orchids". Its flowers are graceful and elegant, and the flowering period is as long as 3 to 4 months. It has high ornamental value and economic value, and is favored by consumers. Under natural conditions, Phalaenopsis mainly reproduces by ramets and seeds, and the reproductive rate is low. Moreover, due to long-term sexual reproduction, the number of virus-carrying strains increases, which gradually reduces the species and affects growth. Nowadays, tissue culture has become the most commonly used method for asexual reproduction of Phalaenopsis. It can not only maintain the excellent traits of the female parent, avoid the generation of mutant plants, but also adapt to it with the advantages of rapi...

Examples

Embodiment 1

[0036] A phalaenopsis pedicel bud cloning medium formula, the medium formula is as follows: MS medium, sugar 20g / L, coconut milk 120ml / L, NAA 5mg / L, 6Ba 20mg / L, citric acid 4g / L, agar 5g / L;

[0037] The pedicel bud cloning culture medium of this Phalaenopsis is made by following method:

[0038] Step S1, mix MS medium and agar, heat to dissolve, then add sugar, coconut milk, NAA, 6Ba and citric acid according to the formula ratio, stir evenly at a speed of 150r / min, and then adjust the pH to 5.3;

[0039] Step S2, dispensing in Erlenmeyer flasks through the medium filling device, the specific steps are as follows:

[0040] Transfer to the filling box, turn on the first motor, the first motor drives the drive shaft to rotate, and then drives the rotating turntable to rotate, and the rotation of the rotating turntable drives the rotating disk to rotate through the limit column, and then drives the feeding tray to rotate, and the culture medium is added to the tank. During pack...

Embodiment 2

[0043] A phalaenopsis pedicel bud cloning medium formula, the medium formula is as follows: MS medium, sugar 30g / L, coconut milk 130ml / L, NAA 10mg / L, 6Ba 22mg / L, citric acid 5g / L, agar 8g / L;

[0044] The pedicel bud cloning culture medium of this Phalaenopsis is made by following method:

[0045]Step S1, mix MS medium and agar, heat to dissolve, then add sugar, coconut milk, NAA, 6Ba and citric acid according to the formula ratio, stir evenly at a speed of 150r / min, and then adjust the pH to 5.3;

[0046] Step S2, dispensing in Erlenmeyer flasks through the medium filling device, the specific steps are as follows:

[0047] Transfer to the filling box, turn on the first motor, the first motor drives the drive shaft to rotate, and then drives the rotating turntable to rotate, and the rotation of the rotating turntable drives the rotating disk to rotate through the limit column, and then drives the feeding tray to rotate, and the culture medium is added to the tank. During pack...

Embodiment 3

[0050] A phalaenopsis pedicel bud cloning medium formula, the medium formula is as follows: MS medium, sugar 40g / L, coconut milk 150ml / L, NAA 12mg / L, 6Ba 25mg / L, citric acid 6g / L, agar 10g / L;

[0051] The pedicel bud cloning culture medium of this Phalaenopsis is made by following method:

[0052] Step S1, mix MS medium and agar, heat to dissolve, then add sugar, coconut milk, NAA, 6Ba and citric acid according to the formula ratio, stir evenly at a speed of 150r / min, and then adjust the pH to 5.3;

[0053] Step S2, dispensing in Erlenmeyer flasks through the medium filling device, the specific steps are as follows:

[0054] Transfer to the filling box, turn on the first motor, the first motor drives the drive shaft to rotate, and then drives the rotating turntable to rotate, and the rotation of the rotating turntable drives the rotating disk to rotate through the limit column, and then drives the feeding tray to rotate, and the culture medium is added to the tank. During pa...

technical field

[0001] The invention belongs to the technical field of phalaenopsis cultivation, in particular to a formula of a phalaenopsis pedicel bud cloning medium. Background technique

[0002] Phalaenopsis belongs to the Orchidaceae plant and has the reputation of "Queen of Orchids". Its flowers are graceful and elegant, and the flowering period is as long as 3 to 4 months. It has high ornamental value and economic value, and is favored by consumers. Under natural conditions, Phalaenopsis mainly reproduces by ramets and seeds, and the reproductive rate is low. Moreover, due to long-term sexual reproduction, the number of virus-carrying strains increases, which gradually reduces the species and affects growth. Nowadays, tissue culture has become the most commonly used method for asexual reproduction of Phalaenopsis. It can not only maintain the excellent traits of the female parent, avoid the generation of mutant plants, but also adapt to it with the advantages of rapi...

Examples

Embodiment 1

[0036] A phalaenopsis pedicel bud cloning medium formula, the medium formula is as follows: MS medium, sugar 20g / L, coconut milk 120ml / L, NAA 5mg / L, 6Ba 20mg / L, citric acid 4g / L, agar 5g / L;

[0037] The pedicel bud cloning culture medium of this Phalaenopsis is made by following method:

[0038] Step S1, mix MS medium and agar, heat to dissolve, then add sugar, coconut milk, NAA, 6Ba and citric acid according to the formula ratio, stir evenly at a speed of 150r / min, and then adjust the pH to 5.3;

[0039] Step S2, dispensing in Erlenmeyer flasks through the medium filling device, the specific steps are as follows:

[0040] Transfer to the filling box, turn on the first motor, the first motor drives the drive shaft to rotate, and then drives the rotating turntable to rotate, and the rotation of the rotating turntable drives the rotating disk to rotate through the limit column, and then drives the feeding tray to rotate, and the culture medium is added to the tank. During pack...

Embodiment 2

[0043] A phalaenopsis pedicel bud cloning medium formula, the medium formula is as follows: MS medium, sugar 30g / L, coconut milk 130ml / L, NAA 10mg / L, 6Ba 22mg / L, citric acid 5g / L, agar 8g / L;

[0044] The pedicel bud cloning culture medium of this Phalaenopsis is made by following method:

[0045]Step S1, mix MS medium and agar, heat to dissolve, then add sugar, coconut milk, NAA, 6Ba and citric acid according to the formula ratio, stir evenly at a speed of 150r / min, and then adjust the pH to 5.3;

[0046] Step S2, dispensing in Erlenmeyer flasks through the medium filling device, the specific steps are as follows:

[0047] Transfer to the filling box, turn on the first motor, the first motor drives the drive shaft to rotate, and then drives the rotating turntable to rotate, and the rotation of the rotating turntable drives the rotating disk to rotate through the limit column, and then drives the feeding tray to rotate, and the culture medium is added to the tank. During pack...

Embodiment 3

[0050] A phalaenopsis pedicel bud cloning medium formula, the medium formula is as follows: MS medium, sugar 40g / L, coconut milk 150ml / L, NAA 12mg / L, 6Ba 25mg / L, citric acid 6g / L, agar 10g / L;

[0051] The pedicel bud cloning culture medium of this Phalaenopsis is made by following method:

[0052] Step S1, mix MS medium and agar, heat to dissolve, then add sugar, coconut milk, NAA, 6Ba and citric acid according to the formula ratio, stir evenly at a speed of 150r / min, and then adjust the pH to 5.3;

[0053] Step S2, dispensing in Erlenmeyer flasks through the medium filling device, the specific steps are as follows:

[0054] Transfer to the filling box, turn on the first motor, the first motor drives the drive shaft to rotate, and then drives the rotating turntable to rotate, and the rotation of the rotating turntable drives the rotating disk to rotate through the limit column, and then drives the feeding tray to rotate, and the culture medium is added to the tank. During pa...

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