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Rapid propagation method of phalaenopsis amabilis flower stalk stem sections

A technology for stem segments and orchids, which is applied in the field of rapid propagation of Phalaenopsis pedicels and stem segments, can solve the problems of death, easy browning and deterioration of explants, etc., so as to reduce the infection rate, improve the reproduction rate, and prevent browning and deterioration. Effect

Inactive Publication Date: 2020-03-24
安徽惠泽园苗木花卉种植有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many researches on the tissue culture of Phalaenopsis, there are still many problems in the tissue culture of Phalaenopsis that have not been improved. In the process of tissue culture, the explants are easy to brown and deteriorate and then die, and prevent the explants from Bacteria contamination is also a relatively common problem during the induction process. In order to overcome the problems in the tissue culture of Phalaenopsis, this application mainly starts from the aspects of tissue culture medium, explant preparation and post-inoculation management to improve the quality of Phalaenopsis tissue culture. Reproductive rate, improve the quality of tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]A method for rapid propagation of phalaenopsis pedicels, comprising the steps of:

[0034] (1) Preparation of medium:

[0035] a. First weigh 13.5 parts of wax gourd rind, 8.5 parts of watermelon rind, 5.5 parts of amaranth stalk, 11 parts of cucumber rind, and 13 parts of pumpkin vine in corresponding weight parts and place them on the wooden sieve together, and then steam fumigate under the wooden sieve. After 32 minutes, take out the mixed raw materials for later use;

[0036] b. Put the mixed raw material after fumigation in operation a into a cryogenic pulverizer for pulverization, after pulverization for 24 minutes, pass through a 50-mesh sieve for later use;

[0037] c. Put the powder sieved in operation b and sterile water into the micro-jet high-pressure homogenizer according to the weight-to-volume ratio of 1g:4.2mL, and then perform homogenization treatment. After homogenization treatment for 11 minutes, use 3 layers of Gauze is filtered to obtain filtrate a...

Embodiment 2

[0058] A method for rapid propagation of phalaenopsis pedicels, comprising the steps of:

[0059] (1) Preparation of medium:

[0060] a. First weigh 14 parts of wax gourd rind, 9 parts of watermelon rind, 6 parts of amaranth stalk, 11.5 parts of cucumber rind, and 13.5 parts of pumpkin vine in corresponding weight parts and place them on a wooden sieve, and then carry out steam fumigation under the wooden sieve. After 35 minutes, take out the mixed raw materials for later use;

[0061] b. Put the mixed raw materials after fumigation in operation a into a cryogenic pulverizer for pulverization, after pulverization for 26 minutes, pass through a 60-mesh sieve for later use;

[0062] c. Put the sieved powder and sterile water in operation b into the micro-jet high-pressure homogenizer according to the weight-to-volume ratio of 1g:4.5mL, and then carry out homogenization treatment. After homogenization treatment for 12 minutes, use 4 layers of Gauze is filtered to obtain filtrat...

Embodiment 3

[0083] A method for rapid propagation of phalaenopsis pedicels, comprising the steps of:

[0084] (1) Preparation of medium:

[0085] a. First weigh 14.5 parts of wax gourd rind, 9.5 parts of watermelon rind, 6.5 parts of amaranth stalk, 12 parts of cucumber rind, and 14 parts of pumpkin vine in corresponding weight parts and put them on a wooden sieve, and then steam fumigate under the wooden sieve, and fumigate After 38 minutes, take out the mixed raw materials for later use;

[0086] b. Put the mixed raw materials after fumigation in operation a into a cryogenic pulverizer for pulverization, after pulverization for 28 minutes, pass through a 70-mesh sieve for later use;

[0087] c. Put the powder sieved in operation b and sterile water into the micro-jet high-pressure homogenizer at a weight-to-volume ratio of 1g:4.8mL, and then perform homogenization treatment. After homogenization treatment for 13 minutes, use 5 layers of Gauze is filtered to obtain filtrate and filter ca...

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PUM

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Abstract

The invention discloses a rapid propagation method of phalaenopsis amabilis flower stalk stem sections. The method comprises the following steps of (1) preparing a culture medium; (2) preparing explants; (3) performing inoculation; and (4) performing management after inoculation. The rapid propagation method of the phalaenopsis amabilis flower stalk stem sections provided by the invention has theadvantages that the browning and deterioration of callus tissues are effectively prevented; the bacterial infection rate of the tissue culture of phalaenopsis amabilis is also greatly reduced; the callus tissue division, differentiation and proliferation capacity is improved; the propagation rate of the tissue culture of the phalaenopsis amabilis is further improved; and a high market popularization value is realized.

Description

technical field [0001] The invention belongs to the technical field of flower planting, and in particular relates to a method for rapid propagation of phalaenopsis pedicels. Background technique [0002] Phalaenopsis( Phalaenopsis amabilis ), also known as Phalaenopsis, is a tropical aerial orchid, a perennial herb. Phalaenopsis is a famous cut flower species and one of the four most commercially valuable ornamental tropical orchids in the world. Phalaenopsis has the reputation of "Queen of Orchids". With the increase of domestic market demand, the production prospect of Phalaenopsis will be better and better, and it has extremely high economic value. Because Phalaenopsis belongs to the uniaxial aerial orchid, the seeds are small, and the embryo and endosperm are not fully developed, so it is not suitable for sowing by traditional methods. Mass propagation of Phalaenopsis seedlings by tissue culture is a fast, convenient and effective way not limited by time. Although th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 吴勇
Owner 安徽惠泽园苗木花卉种植有限公司
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