109results about How to "Reduce the rate of contamination" patented technology

The invention relates to a preparation method by using Chinese medicine residue as a cultivation material of edible mushroom pleurotus ostreatus, which uses the Chinese medicine residue, banana peels, bran, sawdust and lime as raw materials, and is prepared by crushing, insolating, proportioning the materials and sterilizing; specific steps are as follows: respectively crushing the banana peels, the bran and the sawdust into granules; placing the bran, the sawdust and the Chinese medicine residue outdoors to expose to the sun for sterilizing and dewatering; mixing the insolated bran, sawdust and Chinese medicine residue with the crushed banana peels, and then adding water and mixing with the lime sufficiently and uniformly, sterilizing at high temperature to obtain the cultivation material. The preparation method has advantages as follows: the waste Chinese medicine residue from medicine factories and the banana peels are used for culturing high-quality edible mushrooms, the waste is changed into things of value, the production cost is saved, the cultivation period is shortened, the yield is increased, the mushroom contamination rate is reduced with no poison or harm, the eating is safe, the waste treatment difficulty is resolved without causing pollution, both the environmental purpose and the economic purpose are achieved, the operation is simple, and the industrial production is easy to achieve.

The invention discloses a water-culture method for the mouseearcress and comprises the procedures that 1) sprouting of the seed-the seed of the mouseearcress after the yarovization is cultivated in a cultivation room to be a young seedling; 2) a cultivation tool is made; 3) transplanting the seedling-the seedling obtained in the procedure 1) is transplanted to a cultivation pipe obtained in the procedure 2) in the cultivation room and the root of the young seedling contacts the moisture vermiculite; after the young seedling is cultivated for eight to nine days to be a small seedling; 4) culture of seedling-the vermiculite inside the cultivation pipe is primarily removed; Hoagland nutrition solution with one fourth concentration is arranged inside a water tank that is used as the water-culture device; then the cultivation pipe is vertically arranged inside the water tank arranged inside the cultivation room, thereby the root of the small seedling is immersed in the Hoagland nutrition solution. Adopting the method of the invention to cultivate the mouseearcress can effectively improve the survival rate and is convenient and simple to operate.

The present invention discloses coupled deep and shallow layer static fermentation process of producing high fiber coconut fruit. The process includes inoculating pyracetic bacillus to the fermenting culture medium for deep layer static fermentation; and subpacking fermented liquid with wet bacillus concentration of 2.0-3.0 g / l in sterilized shallow containers for shallow layer static fermentation, so as to obtain high fiber coconut fruit with gel film in the required thickness. The production process has shortened shallow layer fermentation period, high production efficiency and less hetero bacterial contamination, and is suitable for industrial production.

The invention relates to pseudomonas aeruginosa and an application thereof. The pseudomonas aeruginosa is named as Pseudomonas aeruginosa YM4 according to bacterial strain classification, and has a preservation number of CCTCC NO:M 2017494. The bacterial strain can produce rhamnolipid in a high yield from hydrophilic carbon sources, hydrophilic carbon sources and composite carbon sources, and therhamnolipid has a substrate conversion rate reach 65-80%. In a rhamnolipid product of the YM4 bacterial strain, di-rhamnolipid and single rhamnolipid are main products, and the amount of di-rhamnolipid and single rhamnolipid takes 80-90% of the total amount of glycolipid. According to the carbon source metabolic characteristic of the bacterial strain, a foam-free seed medium formula suitable for industrial application is developed, and the contamination rate of a seed tank is greatly reduced. The bacterial strain is high in rhamnolipid yield, conversion rate and ratio of a diglycolipid component, and has high industrial application value.

The invention provides a processing method of a starch raw material, which comprises the following steps that: (1) starch raw material powder is mixed with water to obtain starch slurry, and the starch slurry is mixed with amylase; (2) the secondary injection is carried out; (3) by flash vaporization and cooling, a liquefied liquid is obtained after the flash vaporization; (4) the liquefied fluid is in contact with a protein flocculant, and a mixture obtained after the contact keeps stand and is settled and separated to obtain a first liquefied serum layer and a flocculation layer; and (5) the flocculation layer is filter-pressed to obtain a second liquefied serum layer and residues. The invention also provides application of the method in the fermentation preparation of citric acid. The processing method provided by the invention has the advantages of reducing the consumption of energy and equipment of liquefied serum, reducing the production cost and improving the production efficiency.

The invention belongs to the field of genetic engineering of plants and discloses a method for building a high-efficiency regenerating and transforming system of Oryza sativaL. subsp. japonica 11 through cultivation of induction of embryogenic callus, subculture multiplication of embryogenic callus, transformation mediated by agrobacterium tumefaciens, cocultivation of embryogenic callus and agrobacterium tumefaciens, screening and cultivation of resistant callus and seedlings differentiation of resistant callus. The method has the advantages of simple technological processes, low production cost, and capabilities of obviously increasing the inductivity of the embryogenic callus, the subculture multiplication rate and the differentiation and regeneration rate of the embryogenic callus of the Oryza sativaL. subsp. japonica 11 and simultaneously shortening the period of obtaining regenerative rice plants; and on the basis, an improved agrobacterium tumefaciens-mediated method can be used for remarkably increasing the efficiency of obtaining the transgenosis rice plants and shortening the period of obtaining regenerative rice plants.

The invention discloses recombined streptomyces nodosus capable of producing amphotericin B and application thereof. The recombined streptomyces nodosus is obtained by introducing a kanamycin resistance gene sequence showed in SEQ ID NO.1 into streptomyces nodosus ZJB2016050; by introducing the kanamycin resistance gene, produced strains which have no resistance previously have kanamycin resistance, the purity and quality of the strains of seed culture mediums are improved, and strain contamination phenomena can be prevented from occurring during seed cultivation in a lab. By expressing a function gene, namely vitreoscilla hemoglobin (vhb), the yield of AmB is increased by 15%; by expressing a function gene, namely the S- adenosylmethionine synthetase gene (metk), the yield of AmB is increased by about 40%.

The present invention discloses an emulsifying-type aerobic fermentation tank. According to the aerobic fermentation tank in the prior art, increase of compressed air consumption and improvement of stirring motor power can not significantly increase specific yield, and can cause energy consumption increasing, product cost improving, equipment cost increasing and operating environment deterioration. The emulsifying-type aerobic fermentation tank of the present invention comprises a tank body and a gas-liquid mixing circulation apparatus, wherein the gas-liquid mixing circulation apparatus is located inside the tank body, and has at least two stages of gas-liquid mixers. The gas-liquid mixing circulation apparatus is arranged vertically. The tank body is provided with a tank body gas inlet pipe and a fermented liquid inlet pipe. The tank is characterized in that: a self-suction air ejection emulsifying supercharging device is arranged between the gas-liquid mixing circulation apparatus and the inner wall of the tank body. With the tank of the present invention, oxygen utilization rate, mass transfer rate, dissolved oxygen coefficient and specific yield are improved, energy is substantially saved, the equipment is simplified, the microbiological contamination rate is reduced, the equipment cost and the maintenance cost are reduced, and the service life of the fermentation tank isprolonged.

The invention discloses construction and an application of high-yield L-valine engineering bacteria and provides a method for preparing recombinant bacteria. The method comprises steps as follows: an acetohydroxyacid synthase mutant coding DNA module, an acetohydroxyacid isomerism reductase coding DNA module, a branched chain amino acid aminotransferase coding DNA module and a dihydroxy acid dehydrase DNA molecule are introduced into target bacteria and then the recombination bacteria are obtained. An experiment proves that when Brevibacterium flavum MDV-07 is used for fermenting and producing L-valine, the yield of L-valine and a conversion rate can be greatly increased, and great production application value is realized.

The invention relates to a culture utensil, in particular to a culture utensil for large-scale cultivation of cordyceps militaris. The culture utensil for large-scale cultivation of cordyceps militaris comprises a box body and a box lid which is matched with the box body and can integrally seal the box body. The culture utensil is characterized in that the culture utensil is provided with ventilation holes communicating the inside of the culture utensil with the outside, and a seal body which seals the ventilation holes, only allows air to freely pass in and out and performs microfiltration onthe air is also arranged on the culture utensil. The culture utensil solves the problem that as round glass bottles, round plastic bottles and small round plastic boxes cannot replenish oxygen neededduring the growth and development of the cordyceps militaris or discharge redundant moisture in culture mediums or nutrient solution because of impermeability, the air purification of the cordyceps militaris during growth in the culture utensil cannot be guaranteed, resulting in low cordyceps biotransformation rate and germination rate. The invention further improves the shape and material of theculture utensil.

The invention discloses a method for producing stock seeds of enoki mushrooms. The method includes preparing culture media comprising 50-60% of sawdust, 34-45% of rice bran, 4-10% of cottonseed hulls and 0.5-2% of light calcium carbonate and enabling the moisture content of the culture media to be 62-64%; bottling, coring and covering the culture media in an environment with the room temperature lower than 30 DEG C, sterilizing the culture media at the temperature of 110+ / -2 DEG C under the pressure of 0.1-0.12Mpa for 60 minutes, keeping the culture media at the temperature of 121+ / -2 DEG C under the pressure of 0.12-0.13Mpa for 60-90 minutes, and feeding sterilized strain bottles into a cooling chamber to realize forced cooling for the strain bottles until the temperature of each strain bottle ranges from 16 DEG C to 20 DEG C; selecting second-level strains, inoculating the stock seeds of the second-level strains via a solid strain machine; culturing the stock seeds under conditions that the temperature ranges from 17 DEG C 20 DEG C, the humidity ranges from 65% to 70% and the concentration of carbon dioxide ranges from 1000ppm to 2000ppm after the stock seeds are inoculated; and selecting the stock seeds thrice in a culture process and culturing the stock seeds for 16-19 days before the stock seeds can be used. The inoculation amount of the stock seeds is proper so that a material surface can be covered. The method has the advantages that mycelia of the stock seeds are healthy, strong, white and vigorous, and are high in activity, and the strains are not easy to degenerate and are short in growth cycle; the enoki mushrooms can germinate quickly after inoculation and are low in contamination rate and high in yield; and the manufacturing cost is low, and technological conditions are easy to control.

The invention relates to a rotational flow mixer, at least comprising a reaction container for filling liquid, wherein an air inlet is arranged on the container. The rotational flow mixer is characterized in that inner shells are arranged above the air inlet, a gas phase rotational flow passage is formed between the inner shells and the inner wall of the container or between the inner shells, outer shells are arranged above the inner shell, a liquid inlet is arranged in the middle of each outer shell, a fluid rotational flow passage is formed between the outer shells and the inner shells, theouter end parts of the gas phase rotational flow passage and the fluid rotational flow passage are converged into a gas-liquid mixed flow rotational flow passage, and the gas-liquid mixed flow rotational flow passage is located between the outer shells and the inner wall of the container or between the outer shells so that the gas-liquid mixed effect is favourable, a gas-liquid contact area is big, and the rotational flow mixer can be also used as a pneumatic stirrer.

The invention discloses a process for preparing oxytetracycline single colony frozen bacteria, which comprises the following steps of: preparing a plating medium from 3.5 to 4.5 percent of whole meal flour, 0.1 to 0.3 percent of yeast extract powder, 0.1 to 0.3 percent of monopotassium phosphate, 1.5 to 2.5 percent of agar and the balance of water in a certain proportion; cultivating single colony; preparing single colony frozen bacteria from the cultivated single colony; and performing shaking preliminary screening and shaking secondary screening to select the single colony frozen bacteria with strong production capacity to finally prepare freeze-drying tube strains for production. The plating medium can cultivate the single colony with large colony diameter and large numbers of conidium. Single colony spore suspension is directly used for preparing the single colony frozen bacteria. The process of the invention reduces passage times of the strain in the rejuvenation breeding process and the preparation process, prevents strain degeneration, improves the preservation effect and finally fulfills the aim of improving the working efficiency, increasing the yield and reducing the consumption.

The invention discloses a method for high-yield of bottle-cultivated golden mushroom and belongs to the technical field of edible mushrooms. The method comprises steps of (1) bacterial strain selection, (2) ingredient preparation, (3) material packing, disinfection (4), cooling (5), inoculation (6), mycelium culture (7), fruiting management (8) and harvest (9). By the use of the cultivation method, golden mushroom output and quality can be improved, production period can be shortened; golden mushroom marketability can be enhanced; and golden mushroom production efficiency and economic benefits can be improved.

The invention discloses a method for high-efficiency genetic transformation and plant regeneration of pepper, which belongs to the technical field of plant cell engineering, and specifically relates to a method for genetic transformation and plant regeneration mediated by explants of pepper Flamingo bill. The invention improves the culture medium , infection mode, explant cutting mode, delayed selection and improved rooting mode, under the mediation of Agrobacterium, transform Flamingo bill explant, induce, differentiate and plant the callus at the wound of Flamingo bill explant Regeneration and other ways to obtain sustainable growth of genetically transformed regenerated plants, and effectively improve the genetic transformation rate of pepper.

The invention provides pinus koraiensis needle essential oil as well as an extraction method and application thereof in a bacteriostatic culture medium, which belongs to the technical field of plant essential oil extraction. The pinus koraiensis needle essential oil comprises free amino acid and a compound, wherein the content of the free amino acid is 244.44 mg / kg; and calculating at the mass ofthe pinus koraiensis needle essential oil as 100 percent, the compound accounts for 97.92 percent, wherein olefins account for 87.97 percent, alcohols account for 3.7 percent, esters account for 5.77percent, oxides account for 0.24 percent, ketones account for 0.14 percent, and the diterpenes compound accounts for 0.1 percent. The pinus koraiensis needle essential oil obtained by adopting the extraction method contains 46 compounds, so that compared with the existing report, the types of the compounds in the pinus koraiensis needle essential oil can be greatly increased. The pinus koraiensisneedle essential oil is used as a sterilizing agent to be added into the culture medium, so that the bacteria infection rate of the culture medium can be reduced.

The invention discloses a transparent in-situ three-dimensional plant cultivation device and application thereof in plant root system observation. The outside of the device is a transparent cylinder,and the top of a container is sealed by a breathable film; a transparent gel culture medium is contained in the container; a certain space is formed between the transparent gel culture medium and thetop breathable film; the transparent gel culture medium is divided into an upper layer and a lower layer, a layered cultivation mode is adopted, the upper layer has an antibacterial effect, and PPM with the concentration range of 0.005-0.05% in volume percentage concentration which has small influence on root system growth under the condition of ensuring the antibacterial effect is selected, so that the contamination rate of the whole culture medium is inhibited while the root growth is not influenced, the definition of in-situ root system observation can be obviously improved, and ideal results are obtained for dynamic real-time observation and related functional characters obtained after plants are harvested.

The invention relates to a preparation method of an exenatide microsphere preparation. The method comprises the steps of: (1) weighing 5-50wt% of exenatide and 95-50wt% of polyester, which is one of or a mixture of PLGA and PLA at an ester group terminal or carboxyl terminal, with the molecular weight of PLGA being 3.0*10<3>-7*10<4> Dalton and the molecular weight of PLA being 4.0*10<3>-7*10<4> Dalton; (2) dissolving the polyester material in an organic solvent completely to obtain a clear solution, then putting exenatide into the clear solution, and conducting mixing and stirring evenly to obtain a homogeneous oil solution; and (3) supplying the homogeneous oil solution into a high speed rotating disc of an ultra-particle preparation system's (UPPS) droplet generation device by a peristaltic pump to form droplets, and curing the droplets to obtain the microsphere preparation. The microsphere preparation prepared by the method has excellent sustained release performance, a drug loading rate of 35%, an encapsulation rate of above 95%, low burst release rate, a sustained release period of more than 2 weeks, and no release lag period.

The invention discloses a special walnut boiling process. The process comprises the following steps: dispersing 2.8 to 3.0 parts of auxiliary aniseeds, 2.2 to 2.4 parts of pricklyash peels, 2.8 to 3.0 parts of fennel, 1.0 to 1.2 parts of chili pepper, 0.5 to 0.7 part of ginger and 25 to 27 parts of table salt by utilizing hot water to obtain a mixed solution, re-boiling the mixed solution, soaking 282 to 284 parts of walnuts in the mixed solution, finally spinning the water of the walnuts, draining the water of the walnuts to obtain the weird-flavor walnuts, carrying out vacuum packaging on the walnuts, sterilizing the walnuts at high temperature and high pressure, storing the packed walnuts at normal temperature, wherein the shelf life can reach 180 days. By adopting the weird boiling process, the dry and rough taste of the original-flavor walnut can be overcome, the nutrients of the original-flavor walnut can be reserved, and the edible value is high.

The invention provides an aseptic sampling device for a biological sample. The aseptic sampling device comprises a hollow fiber membrane module, an air supply unit and a sampling tool, wherein an air inlet pipe and an air outlet are formed in the hollow fiber membrane module; the sampling tool is fixed on the air outlet; the air supply unit is connected with the air inlet pipe; the hollow fiber membrane module comprises a hollow fiber hydrophobic membrane; the inner diameter of the hollow fiber hydrophobic membrane is 0.8+ / -0.5mm; the thickness of a membrane fiber is 0.15mm; the porosity is 85%; and the aperture is 0.1-0.16mu m. The aseptic sampling device has the beneficial effects that the air inlet pipe in the device can be externally connected with air supply equipment, so that the functions of extremophile bacteria in isolated air during the period of sampling the biological sample are achieved.

The invention provides a capsicum annuum L. anther tissue culture method. The method comprises steps as follows: A), capsicum annuum L. flower buds are collected, and calyx sepals in the position 1 / 4-3 / 4 away from the top of each calyx are removed; B), the flower buds are disinfected; C), anther in the flower buds is inoculated to a culture medium; and D), the anther in the step C) is cultivated. The invention further provides a capsicum annuum L. anther tissue culture method which comprises steps as follows: a), capsicum annuum L. flower buds are collected; b), the flower buds are disinfected; c), anther in the flower buds is inoculated to a culture medium, wherein the culture medium contains one or more of a capsicum annuum L. flower bud crude extract, a capsicum annuum L. anther crude extract, a capsicum annuum L. sterile seedling leaf crude extract, a capsicum annuum L. seed crude extract, a garlic crude extract and a bitter gourd polypeptide crude extract, and preferably, the culture medium contains a mixed solution of the capsicum annuum L. anther crude extract and the bitter gourd polypeptide crude extract; and d), the anther in the step c) is cultivated.

The invention relates to an organic large flower mushroom cultivation method which includes the steps: batching; filling of a bag with mixing materials; sterilization; deep hole inoculation; fruitingmanagement; storage management. According to the method, deep hole inoculation is implemented, so that cylindrical hyphae are formed in holes, fungi are stereoscopically conveyed, spawn running is accelerated, inoculation openings are less, contamination rate is reduced, and fungus bag finished product ratio is increased. Compared with a traditional flower mushroom with the maximum umbrella coverdiameter of about 8cm, the umbrella cover diameter of an organic large flower mushroom can reach 12cm, 2 organic large flower mushrooms can be grown in a batch, labor management cost is reduced, and the organic large flower mushrooms are high in quality and yield. Characteristic cultivation and production of the large flower mushrooms can achieve leisure picking economy, and peasant household income can be improved.

A swirl mixer, at least including a liquid-filled reaction container, the container is provided with an air inlet, characterized in that: an inner shell is provided above the air inlet, the inner shell and the inner wall of the container or the inner shell A gas phase swirl channel is formed between the body and the inner shell, an outer shell is arranged above the inner shell, a liquid inlet is opened in the middle of the outer shell, a fluid swirl channel is formed between the outer shell and the inner shell, and the gas phase swirl flow The outer end of the channel and the fluid swirl channel merges into a gas-liquid mixed flow swirl channel, and the gas-liquid mixed flow swirl channel is located between the outer shell and the inner wall of the container or between the outer shell and the outer shell, so that the gas-liquid mixing effect is good , The gas-liquid contact area is large, and the swirl mixer can also be used as a pneumatic agitator.

The invention provides a method of physically assisting and facilitating plant regeneration of aleurites fordii stem segments with buds. The method includes the following steps of S1, conducting primary culture, wherein after being disinfected, aleurites fordii seed embryos are used for inoculating a primary culture medium untill the seed embryos bud, and the primary culture medium comprises 1 / 2 of MS and 0.5-1.0 mg / L of GA3, or 1 / 2 of MS and 0.5 mg / L of IAA; S2, inducing the stem segments with the buds to bud, wherein the stem segments with the buds on the aleurites fordii seed embryos in step 1 are cut off and then used for inoculating an induction medium to be induced to bud, and the induction medium comprises MS, 3 mg / L of 6-BA and 0.1 mg / L of IBA; S3, conducting rooting treatment, wherein the stem segments with new buds are transferred into a rooting medium, and the rooting medium comprises 1 / 2 of MS, 0.2 mg / L of LIBA, 0.5 g / L of activated carbon and medical stone which accounts for 1 / 10 of the volume of a container. According to the method of conducting in-vitro rapid propagation on the aleurites fordii stem segments with the buds, by obtaining the stem segments with the budsafter the seed embryos bud aseptically, the bacterium infection rate is greatly reduced, and the budding rate is increased; during activation and proliferation of the stem segments with the buds, thesame culture medium is adopted, and by carrying out light quality control on proliferation indexes, the workload of preparing different culture media is reduced; by adding additives into the rootingmedium, the rooting rate is greatly increased.

The invention discloses a preparation method for an aspergillus niger spore suspension liquid. The method comprises preparing the aspergillus niger spore suspension liquid from aspergillus niger spore, and the pH value of the aspergillus niger spore suspension liquid is 1-2.5. The invention also discloses a storage method for aspergillus niger spore. The method comprises preparing the aspergillus niger spore suspension liquid from aspergillus niger spore, and the preparation method of the aspergillus niger spore suspension liquid is the method provided by the invention. By employing the methods disclosed by the invention, the bacterium-infection rate of aspergillus niger spore in the storage stage can be effectively reduced, so that the quality of a aspergillus niger seed liquid can be improved, and the fermentation level of citric acid can be improved.

The invention discloses a genetic transformation method of larch embryonic cells. The method sequentially comprises the following steps: (a1) taking larch embryonic cells, and suspending in a recombinant agrobacterium infection solution to perform infection; (a2) after completing the step (a1), collecting the larch embryonic cellsa, and carrying out recovery culture; and (a3) after completing the step (a2), taking the larch embryonic cells, and removing bacteria by a vacuum-filtration bacterium remover. The method disclosed by the invention can be used for carrying out rapid oriented genetic improvement on larch by gene engineering means, and provides a technical platform for researching the control on expression of exogenous genes in larch, and functional verification and embryonic development.

The invention relates to a preparation method of coenzyme Q10, in particular to a method for producing coenzyme Q10 by industrial fermentation. A strain used in the method is Rhodobacter sphaeroides.The method includes the following steps: plate culture of the strain, inclined plane culture of the strain, 20 L seed culture, primary seed tank culture, secondary seed culture, tertiary fermentationculture, seed-pouring culture and fermentation. The method can solve problems that a culture medium in fermentation liquor is incompletely consumed and the fermentation liquor has high impurity content, low effective units, and accordingly low in refining yield, the quality of industrial salt is not easy to control, low-unit hyphae causes low yield and high sewage discharge, and unused organic matter in emissions causes excessive COD emissions, thereby increasing environmental protection costs. The method has simple process flow and low bacteria contamination risk, saves fermentation cost, improves fermentation output, increases process reserves, and increases production scheduling.

The invention discloses a monellin protein mutant and a preparation method thereof. Site-specific mutagenesis is performed on a single-chain monellin, a thermostable mutant is obtained and successfully expressed in pichia pastoris, and the technical problem that sweet protein is easy to degrade in the production and transportation process is solved; meanwhile, protein sweetness is improved, the good sweet effect can be achieved on the condition of a small amount of monellin protein mutant, and a solid foundation is laid for large-scale industrial marketization of the sweet protein.

The invention provides a method for producing hirsutella sinensis, belonging to the field of biological fermentation. The method is applied to production of cordyceps sinensis, and comprises the following steps: (1) culturing a shaker; (2) culturing a fermentation seed tank; (3) carrying out third-stage and fourth-stage fermenting culture, supplementing materials until the total volume of the tank is 70-80 percent after 3-5 days of culturing; and (4) discharging, and filtering to obtain the hirsutella sinensis mycelium. The method has the beneficial effects that the fermenting cycle is shortened from existing total cycle of 38-42 days to 30-35 days, the risk of bacterial contamination is reduced since the general fermenting cycle is shortened, the consumption is reduced, the product yield is increased from existing 7% to 9%, and each quality index of the product completely reaches the requirement of pharmacopeia standards.