The invention provides a method of physically assisting and facilitating plant regeneration of aleurites fordii stem segments with buds. The method includes the following steps of S1, conducting primary culture, wherein after being disinfected, aleurites fordii seed embryos are used for inoculating a primary culture medium untill the seed embryos bud, and the primary culture medium comprises 1/2 of MS and 0.5-1.0 mg/L of GA3, or 1/2 of MS and 0.5 mg/L of IAA; S2, inducing the stem segments with the buds to bud, wherein the stem segments with the buds on the aleurites fordii seed embryos in step 1 are cut off and then used for inoculating an induction medium to be induced to bud, and the induction medium comprises MS, 3 mg/L of 6-BA and 0.1 mg/L of IBA; S3, conducting rooting treatment, wherein the stem segments with new buds are transferred into a rooting medium, and the rooting medium comprises 1/2 of MS, 0.2 mg/L of LIBA, 0.5 g/L of activated carbon and medical stone which accounts for 1/10 of the volume of a container. According to the method of conducting in-vitro rapid propagation on the aleurites fordii stem segments with the buds, by obtaining the stem segments with the budsafter the seed embryos bud aseptically, the bacterium infection rate is greatly reduced, and the budding rate is increased; during activation and proliferation of the stem segments with the buds, thesame culture medium is adopted, and by carrying out light quality control on proliferation indexes, the workload of preparing different culture media is reduced; by adding additives into the rootingmedium, the rooting rate is greatly increased.