Capsicum annuum L. anther tissue culture method

Abstract

The invention provides a capsicum annuum L. anther tissue culture method. The method comprises steps as follows: A), capsicum annuum L. flower buds are collected, and calyx sepals in the position 1 / 4-3 / 4 away from the top of each calyx are removed; B), the flower buds are disinfected; C), anther in the flower buds is inoculated to a culture medium; and D), the anther in the step C) is cultivated. The invention further provides a capsicum annuum L. anther tissue culture method which comprises steps as follows: a), capsicum annuum L. flower buds are collected; b), the flower buds are disinfected; c), anther in the flower buds is inoculated to a culture medium, wherein the culture medium contains one or more of a capsicum annuum L. flower bud crude extract, a capsicum annuum L. anther crude extract, a capsicum annuum L. sterile seedling leaf crude extract, a capsicum annuum L. seed crude extract, a garlic crude extract and a bitter gourd polypeptide crude extract, and preferably, the culture medium contains a mixed solution of the capsicum annuum L. anther crude extract and the bitter gourd polypeptide crude extract; and d), the anther in the step c) is cultivated.

Description

technical field [0001] The invention relates to the field of tissue culture, in particular to a method for tissue culture of capsicum anther. Background technique [0002] Pepper (Capsicum annuum L.) anther culture is an important assisted breeding technology in the creation of pepper germplasm resources. This technology can not only shorten the time required for conventional hybrid breeding, improve the efficiency of seed selection, but also fully express various gametes. The combined genotype type has great potential in breeding and genetic engineering research. [0003] Pepper anther culture research started earlier, and many breakthroughs have been made so far. In 1973, Wang Yuying, Kuo and George reported that haploid plants of pepper were obtained by anther culture. Subsequent scholars have done a lot of research on the material genotype, the composition of the medium, and the culture conditions, and have made some progress. In China, anther culture has been applied...

AI Technical Summary

Problems solved by technology

Especially in southern China, due to the high temperature and rainy summer, various fungi breed in large quantities. Although the pepper anthers are most suitable for cultivation to produce embryoids at this time, the method of disinfecting the surface of the flower buds cannot kill the inner fungus contained in the flower buds at all. Bacteria, the high pollution rate has led to a significant increase in the cost of anther culture, resulting in a great waste of manpower, material and financial resources

There are also experimenters who add antibiotics to the culture medium to inhibit anther growth. However, although antibiotics can reduce the rate of anther infection, even low concentrations of antibiotics can easily cause anther browning and death, and ultimately fail to produce embryoid bodies.

The method adopted is to add vitamin C, silver nitrate, activated carbon, etc. in the culture medium, but the effect is not ideal.

Images