Method for producing hirsutella sinensis
A technology of Mortierella chinensis and production method, which is applied in the production field of Mortierella chinensis by liquid submerged fermentation, can solve the problems of poor quality of Cordyceps sinensis, long production cycle, low nutrient content, etc., to reduce the infection rate, The effect of reducing incubation time
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Embodiment 1
[0015] Embodiment 1, the method contains the steps:
[0016] (1) Shaker culture
[0017] The medium by weight percentage is: 1% of silkworm chrysalis, 1% of corn, 1% of bran, 0.1% of peptone, 1% of glucose, 0.01% of magnesium sulfate, 0.01% of potassium dihydrogen phosphate, a trace amount of defoamer, and the rest is water; The pH value of sodium hydroxide is adjusted to 7.0-7.5, the pressure of sterile air is 0.11-0.15MPa, and the ventilation ratio is 0.5VVm; cultured at 20°C for 8 days;
[0018] (2) Fermentation seed tank cultivation
[0019] The primary and secondary fermentation media are: glucose 1%, yeast extract 1%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.01%, a trace amount of antifoaming agent, and the rest are water; the medium content is a fermenter 70% of the total volume, adjust the pH value to 7.0-7.5 with sodium hydroxide; the primary inoculation volume is 5%, (the proportion of the seed liquid to the medium) the secondary inoculation volume...
Embodiment 2
[0024] Embodiment 2, the method contains the steps:
[0025] (1) Shaker culture
[0026] The medium by weight percentage is: silkworm chrysalis 3%, corn 3%, bran 3%, peptone 1%, glucose 2%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%, a trace amount of defoamer, and the rest is water; The pH value of sodium hydroxide is adjusted to 7.0-7.5, the pressure of sterile air is 0.11-0.15MPa, and the ventilation ratio is 1.5VVm; cultured at 15°C for 9 days;
[0027] (2) Fermentation seed tank cultivation
[0028] The primary and secondary fermentation media are: glucose 3%, yeast extract 3%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%, a trace amount of defoamer, and the rest are water; the medium content is a fermenter 80% of the total volume, adjust the pH value to 7.0-7.5 with sodium hydroxide; the primary inoculation volume is 10%, (the proportion of the seed liquid to the medium) the secondary inoculation volume is 15%, and the pressure of th...
Embodiment 3
[0033] Embodiment 3, the method contains the steps:
[0034] (1) Shaker culture
[0035] The medium by weight percentage is: silkworm chrysalis 2%, corn 2%, bran 2%, peptone 0.5%, glucose 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.03%, a trace amount of defoamer, and the rest is water; The pH value is adjusted to 7.0-7.5 by sodium hydroxide, the pressure of sterile air is 0.11-0.15MPa, and the ventilation ratio is 1.0VVm; cultured at 18°C for 10 days;
[0036] (2) Fermentation seed tank cultivation
[0037] The primary and secondary fermentation media are: glucose 2%, yeast extract 2%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.03%, a trace amount of antifoaming agent, and the rest are water; the medium content is a fermenter 75% of the total volume, adjust the PH value to 7.0-7.5 with sodium hydroxide; the primary inoculation volume is 7%, (the proportion of the seed liquid to the medium) the secondary inoculation volume is 11%, and the...
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