Method for producing hirsutella sinensis

A technology of Mortierella chinensis and production method, which is applied in the production field of Mortierella chinensis by liquid submerged fermentation, can solve the problems of poor quality of Cordyceps sinensis, long production cycle, low nutrient content, etc., to reduce the infection rate, The effect of reducing incubation time

Inactive Publication Date: 2017-05-31
青海珠峰冬虫夏草工程技术研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cordyceps sinensis anamorphic strain Hirsutella sinensis (Hirsutella sinensis Lu, Guo, Yu et Zeng), many individuals and units in China are currently researching and publishing Cordyceps sinensis culture medium and fermentation production methods, however, these technologies mostly use common culture medium for microbial fermentation, Basically, eggs, milk, and potatoes are used as food sources for fermentation. Although the selection of medium components is b

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] Embodiment 1, the method contains the steps:

[0016] (1) Shaker culture

[0017] The medium by weight percentage is: 1% of silkworm chrysalis, 1% of corn, 1% of bran, 0.1% of peptone, 1% of glucose, 0.01% of magnesium sulfate, 0.01% of potassium dihydrogen phosphate, a trace amount of defoamer, and the rest is water; The pH value of sodium hydroxide is adjusted to 7.0-7.5, the pressure of sterile air is 0.11-0.15MPa, and the ventilation ratio is 0.5VVm; cultured at 20°C for 8 days;

[0018] (2) Fermentation seed tank cultivation

[0019] The primary and secondary fermentation media are: glucose 1%, yeast extract 1%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.01%, a trace amount of antifoaming agent, and the rest are water; the medium content is a fermenter 70% of the total volume, adjust the pH value to 7.0-7.5 with sodium hydroxide; the primary inoculation volume is 5%, (the proportion of the seed liquid to the medium) the secondary inoculation volume...

Example Embodiment

[0024] Embodiment 2, the method contains the steps:

[0025] (1) Shaker culture

[0026] The medium by weight percentage is: silkworm chrysalis 3%, corn 3%, bran 3%, peptone 1%, glucose 2%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%, a trace amount of defoamer, and the rest is water; The pH value of sodium hydroxide is adjusted to 7.0-7.5, the pressure of sterile air is 0.11-0.15MPa, and the ventilation ratio is 1.5VVm; cultured at 15°C for 9 days;

[0027] (2) Fermentation seed tank cultivation

[0028] The primary and secondary fermentation media are: glucose 3%, yeast extract 3%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%, a trace amount of defoamer, and the rest are water; the medium content is a fermenter 80% of the total volume, adjust the pH value to 7.0-7.5 with sodium hydroxide; the primary inoculation volume is 10%, (the proportion of the seed liquid to the medium) the secondary inoculation volume is 15%, and the pressure of th...

Example Embodiment

[0033] Embodiment 3, the method contains the steps:

[0034] (1) Shaker culture

[0035] The medium by weight percentage is: silkworm chrysalis 2%, corn 2%, bran 2%, peptone 0.5%, glucose 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.03%, a trace amount of defoamer, and the rest is water; The pH value is adjusted to 7.0-7.5 by sodium hydroxide, the pressure of sterile air is 0.11-0.15MPa, and the ventilation ratio is 1.0VVm; cultured at 18°C ​​for 10 days;

[0036] (2) Fermentation seed tank cultivation

[0037] The primary and secondary fermentation media are: glucose 2%, yeast extract 2%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.03%, a trace amount of antifoaming agent, and the rest are water; the medium content is a fermenter 75% of the total volume, adjust the PH value to 7.0-7.5 with sodium hydroxide; the primary inoculation volume is 7%, (the proportion of the seed liquid to the medium) the secondary inoculation volume is 11%, and the...

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PUM

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Abstract

The invention provides a method for producing hirsutella sinensis, belonging to the field of biological fermentation. The method is applied to production of cordyceps sinensis, and comprises the following steps: (1) culturing a shaker; (2) culturing a fermentation seed tank; (3) carrying out third-stage and fourth-stage fermenting culture, supplementing materials until the total volume of the tank is 70-80 percent after 3-5 days of culturing; and (4) discharging, and filtering to obtain the hirsutella sinensis mycelium. The method has the beneficial effects that the fermenting cycle is shortened from existing total cycle of 38-42 days to 30-35 days, the risk of bacterial contamination is reduced since the general fermenting cycle is shortened, the consumption is reduced, the product yield is increased from existing 7% to 9%, and each quality index of the product completely reaches the requirement of pharmacopeia standards.

Description

technical field [0001] The invention relates to a production method of Trichospora sinensis, in particular to a production method of Trichospora sinensis by liquid submerged fermentation. Background technique [0002] Cordyceps sinensis is a second-class protected species in my country and a precious treasure of Chinese medicinal materials in China. It has been popularized and applied in my country for more than 2,000 years. Cordyceps sinensis is not only a medicinal fungus with good health care function, but also a high-grade nourishing edible fungus with high nutritional value. With the improvement of people's living standards and quality of life, the demand for functional health care products is also expanding. Due to the narrow distribution area of ​​wild Cordyceps sinensis, low natural parasitic rate and harsh living environment conditions, its own resources are relatively limited. In recent years, the ecological environment of the main producing areas of Cordyceps sin...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 王玉花王辉
Owner 青海珠峰冬虫夏草工程技术研究有限公司
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