Method for producing coenzyme Q10 by industrial fermentation

Abstract

The invention relates to a preparation method of coenzyme Q10, in particular to a method for producing coenzyme Q10 by industrial fermentation. A strain used in the method is Rhodobacter sphaeroides.The method includes the following steps: plate culture of the strain, inclined plane culture of the strain, 20 L seed culture, primary seed tank culture, secondary seed culture, tertiary fermentationculture, seed-pouring culture and fermentation. The method can solve problems that a culture medium in fermentation liquor is incompletely consumed and the fermentation liquor has high impurity content, low effective units, and accordingly low in refining yield, the quality of industrial salt is not easy to control, low-unit hyphae causes low yield and high sewage discharge, and unused organic matter in emissions causes excessive COD emissions, thereby increasing environmental protection costs. The method has simple process flow and low bacteria contamination risk, saves fermentation cost, improves fermentation output, increases process reserves, and increases production scheduling.

Description

technical field

[0001] The invention relates to a preparation method of coenzyme Q10, in particular to a method for producing coenzyme Q10 by industrial fermentation. Background technique

[0002] Coenzyme Q10 (CoenzymeQ10, CoQ10), also known as ubiquinone, is a vitamin-like substance that exists in the cells of animals, plants, microorganisms, etc. at very low levels. Because coenzyme Q10 is a natural antioxidant and cell metabolism activator synthesized by cells itself, it can protect proteins and DNA molecules from free radical-induced oxidation, so it is widely used in pharmaceuticals, cosmetics, food and other industries. At present, coenzyme Q10 is a good clinical biochemical drug in medicine, and it is often used in the comprehensive treatment of liver disease, cardiovascular disease, cancer and improving the body's immunity. In the field of food, as a new type of food nutritional additive, it can enhance physical fitness and improve immunity. At the same time, coen...

Examples

Embodiment 1

[0048] Embodiment 1 "One Belt Two" Planting Process Method

[0049] Plate culture of S1 strain: After preparing the medium according to the formula of medium A and sterilizing, pour the plate under aseptic conditions. Pipette 1 mL of glycerol bacterial suspension into a test tube filled with 9 mL of sterile water with a pipette, shake on a vortex shaker, disperse and mix, and then use a pipette to double-dilute. Use a dilution of 10 -5 Inoculate the plate with 200-300 μL of bacterial suspension in each plate and spread evenly with a sterilized spreader. Put the evenly coated plate into an incubator with a temperature of 32.0±0.5°C and a humidity of 35%-45% for 5-7 days.

[0050] Slope culture of strain S2: After preparing the medium according to the formula of medium A and sterilizing it, pour the slope under aseptic conditions. From the mature mother plate, use an inoculation shovel to select a few full, raised, and uniformly sized single colonies, transfer them into a tes...

Embodiment 2

[0058] Example 2 Implementation of "two-band three" seeding process

[0059] In the early stage, the fermentation process control of "flat seeds-slanted seeds-20 L seeds- primary seeds- secondary seeds" was basically the same. In the "two with three" inverted seed re-cultivation process, the fermenter that has been fermented for 45-65 hours and tested to meet the pure-bred culture standard is selected as the fermentation liquid supply tank, and the fermentation liquid in it is equally divided into another sterilization completed. continued cultivation in the receiving tank. by attaching image 3 It can be seen that the potency of the "two belt three" pouring process is slightly higher than that of the original process, mainly because the volume of the fermentation broth is reduced after the two belts are put in the tank to effectively improve the fermenter's filling coefficient and increase the fermentation dissolved oxygen. level, thereby promoting the improvement of the po...

technical field

[0001] The invention relates to a preparation method of coenzyme Q10, in particular to a method for producing coenzyme Q10 by industrial fermentation. Background technique

[0002] Coenzyme Q10 (CoenzymeQ10, CoQ10), also known as ubiquinone, is a vitamin-like substance that exists in the cells of animals, plants, microorganisms, etc. at very low levels. Because coenzyme Q10 is a natural antioxidant and cell metabolism activator synthesized by cells itself, it can protect proteins and DNA molecules from free radical-induced oxidation, so it is widely used in pharmaceuticals, cosmetics, food and other industries. At present, coenzyme Q10 is a good clinical biochemical drug in medicine, and it is often used in the comprehensive treatment of liver disease, cardiovascular disease, cancer and improving the body's immunity. In the field of food, as a new type of food nutritional additive, it can enhance physical fitness and improve immunity. At the same time, coen...

Examples

Embodiment 1

[0048] Embodiment 1 "One Belt Two" Planting Process Method

[0049] Plate culture of S1 strain: After preparing the medium according to the formula of medium A and sterilizing, pour the plate under aseptic conditions. Pipette 1 mL of glycerol bacterial suspension into a test tube filled with 9 mL of sterile water with a pipette, shake on a vortex shaker, disperse and mix, and then use a pipette to double-dilute. Use a dilution of 10 -5 Inoculate the plate with 200-300 μL of bacterial suspension in each plate and spread evenly with a sterilized spreader. Put the evenly coated plate into an incubator with a temperature of 32.0±0.5°C and a humidity of 35%-45% for 5-7 days.

[0050] Slope culture of strain S2: After preparing the medium according to the formula of medium A and sterilizing it, pour the slope under aseptic conditions. From the mature mother plate, use an inoculation shovel to select a few full, raised, and uniformly sized single colonies, transfer them into a tes...

Embodiment 2

[0058] Example 2 Implementation of "two-band three" seeding process

[0059] In the early stage, the fermentation process control of "flat seeds-slanted seeds-20 L seeds- primary seeds- secondary seeds" was basically the same. In the "two with three" inverted seed re-cultivation process, the fermenter that has been fermented for 45-65 hours and tested to meet the pure-bred culture standard is selected as the fermentation liquid supply tank, and the fermentation liquid in it is equally divided into another sterilization completed. continued cultivation in the receiving tank. by attaching image 3 It can be seen that the potency of the "two belt three" pouring process is slightly higher than that of the original process, mainly because the volume of the fermentation broth is reduced after the two belts are put in the tank to effectively improve the fermenter's filling coefficient and increase the fermentation dissolved oxygen. level, thereby promoting the improvement of the po...

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