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Water-planting method for arabidopsis thaliana

A kind of Arabidopsis thaliana and hydroponics technology, applied in the field of cultivating Arabidopsis thaliana, can solve the problems of easy to infect bacteria, dry up, etc., and achieve the effect of high survival rate, not easy to infect bacteria, and low price

Inactive Publication Date: 2008-03-19
ZHEJIANG UNIV
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Problems solved by technology

Among them, hydroponics is a common cultivation method in the laboratory; however, there are certain deficiencies in the hydroponics methods reported at home and abroad, and its biggest disadvantage is that it is easy to infect bacteria or dry out

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  • Water-planting method for arabidopsis thaliana

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Embodiment Construction

[0020] A hydroponic method of Arabidopsis thaliana, the following steps are carried out in sequence:

[0021] 1), germination of seeds:

[0022] The gained seeds of Arabidopsis thaliana after vernalization were sterilized with 75% alcohol for 10 min, washed 3 times with sterile water, and then sowed on the sterilized MS medium located in the petri dish (the MS medium without sucrose, See Table 1), adjust the pH to 5.7, and the agarose concentration is 0.55%. After sowing, wrap the Petri dish with Parafilm sealing film to prevent water from evaporating and avoid bacterial contamination. Then place it in the cultivation room for 4 to 5 days, and when the root length is about 1 cm, it becomes a seedling 4, which can be used for transplanting seedlings. The culture conditions are temperature 20-28°C, humidified with an indoor humidifier until the humidity is 60%-80%; the light source adopts a biological effect lamp composed of white light and red light intervals, and the turn-on...

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Abstract

The invention discloses a water-culture method for the mouseearcress and comprises the procedures that 1) sprouting of the seed-the seed of the mouseearcress after the yarovization is cultivated in a cultivation room to be a young seedling; 2) a cultivation tool is made; 3) transplanting the seedling-the seedling obtained in the procedure 1) is transplanted to a cultivation pipe obtained in the procedure 2) in the cultivation room and the root of the young seedling contacts the moisture vermiculite; after the young seedling is cultivated for eight to nine days to be a small seedling; 4) culture of seedling-the vermiculite inside the cultivation pipe is primarily removed; Hoagland nutrition solution with one fourth concentration is arranged inside a water tank that is used as the water-culture device; then the cultivation pipe is vertically arranged inside the water tank arranged inside the cultivation room, thereby the root of the small seedling is immersed in the Hoagland nutrition solution. Adopting the method of the invention to cultivate the mouseearcress can effectively improve the survival rate and is convenient and simple to operate.

Description

technical field [0001] The invention relates to a method for cultivating Arabidopsis, in particular to a hydroponic method for Arabidopsis. Background technique [0002] Arabidopsis is the first higher plant in the world to complete the sequencing of its entire genome, and the use of Arabidopsis as a model plant for plant functional genomics research is in full swing in countries all over the world. Using Arabidopsis thaliana to carry out botany research can provide basic experimental materials for mining information on various metabolic pathways in plants, discovering and cloning important genes. At present, the culture methods of Arabidopsis mainly include the mixed nutrient medium of peat and vermiculite and the culture of nutrient solution. Among them, hydroponics is a common cultivation method in the laboratory; however, there are certain deficiencies in the hydroponics methods reported at home and abroad, and its biggest disadvantage is that it is easy to infect bacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G31/02
CPCY02P60/21
Inventor 都韶婷章永松林咸永
Owner ZHEJIANG UNIV
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