112results about How to "Not easy to infect bacteria" patented technology

The invention discloses a water-culture method for the mouseearcress and comprises the procedures that 1) sprouting of the seed-the seed of the mouseearcress after the yarovization is cultivated in a cultivation room to be a young seedling; 2) a cultivation tool is made; 3) transplanting the seedling-the seedling obtained in the procedure 1) is transplanted to a cultivation pipe obtained in the procedure 2) in the cultivation room and the root of the young seedling contacts the moisture vermiculite; after the young seedling is cultivated for eight to nine days to be a small seedling; 4) culture of seedling-the vermiculite inside the cultivation pipe is primarily removed; Hoagland nutrition solution with one fourth concentration is arranged inside a water tank that is used as the water-culture device; then the cultivation pipe is vertically arranged inside the water tank arranged inside the cultivation room, thereby the root of the small seedling is immersed in the Hoagland nutrition solution. Adopting the method of the invention to cultivate the mouseearcress can effectively improve the survival rate and is convenient and simple to operate.

The invention discloses a novel application of malt oligosaccharide based mycose synthetase and malt oligosaccharide based mycose hydrolase in mycose production. The malt oligosaccharide based mycose synthetase has an amino acid sequence as shown in SEQ ID NO:1; and the malt oligosaccharide based mycose hydrolase has the amino acid sequence as shown in SEQ ID NO:3. The malt oligosaccharide based mycose synthetase and malt oligosaccharide based mycose hydrolase disclosed in the invention also have higher most-suitable reaction temperature and thermal stabilities, and lower most-suitable pH values, so that contamination risks are lowered, production stability is improved, the mycose production efficiency by acting reducing glucidtemns through the malt oligosaccharide based mycose synthetase and malt oligosaccharide based mycose hydrolase and pullulanase in a combined manner is improved and the mycose cost is lowered, therefore, a solid foundation is laid up for the extensive application of the mycose in medicines, foods and cosmetics.

The invention discloses a probiotic agent containing Lactobacillus plantarum and Bifidobacterium lactis, and its preparing method, and belongs to the technical field of feed microbes. The probiotic agent having the advantages of special sour fragrance, high number of contained live bacteria and improved degradation rate of proteins in the soybean meal, wheat bran and corn flour is prepared through the solid-state fermentation of soybean meal, wheat bran and corn flour by adopting Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9; and the method has the characteristics of low production cost and simple operation. The adoption of mixed bacterium segment solid state fermentation can utilize the mutualism synergism of strains, and can improve the yield and the thallus concentration; and the probiotic agent utilizing the growth, propagation and metabolism of the Lactobacillus plantarum HM-10 in the early stage of fermentation can provide abundant nutrients and necessary anaerobic environment for the propagation of the Bifidobacterium lactis V9, so it is helpful for the growth of the Bifidobacterium lactis V9, and the number of Lactobacillus plantarum HM-10 live bacteria is increased.

The invention discloses recombinant human bone morphogenetic protein-2 (rhBMP-2)-loaded chitosan microspheres, a preparation method thereof and application thereof. A substrate of the rhBMP-2-loaded chitosan microspheres is chitosan microspheres, and a loaded medicament is rhBMP-2. Chitosan reacts with an ionic cross-linking agent, and a reaction product is processed by solution of sodium hydroxide to prepare the chitosan microspheres; and the rhBMP-2 is loaded by a passive loading mode, so that the rhBMP-2 is adsorbed on the chitosan microspheres, wherein a weight ratio of the rhBMP-2 to thechitosan is (1-30):100. The preparation method is simple and easy, has a mild preparation condition and is favorable for maintaining the bioactivity of the rhBMP-2. The rhBMP-2-loaded chitosan microspheres can control and release the rhBMP-2 for a long term, promote the formation of new bones, and can be used for preparing medicaments for treating bone coloboma, nonunion and delayed union.

The invention relates to a preparing method and application of a composite biological agent for agricultural root-knot nematodes. The biological agent is obtained through solid fermentation of paecilomyces lilacinus YT08 with the preservation number of CG MCC No.10026 and has a powerful inhibitory effect on the agricultural root-knot nematode disease. A preferred culture medium involved in solid fermentation is prepared from 17-30% of dried brewer grain, 4-8% of bran, 3-6% of smashed peanut shells, 2-5% of soy peptone, 2.55-7.5% of powder chitosan (with the deacetylation degree larger than or equal to 90%), 1-2.25% of crab shell meal, 0.2-0.5% of NaCl, 0.1-0.3% of CaCl2, 0.1-0.3% of MgSO4, 0.05-0.15% of FeSO4 and 40-70% of water. The preparing method has the advantages of being easy to operate and control and suitable for industrial production, the raw materials are low in cost, and the composite biological agent has the powerful effect.

The invention discloses a taxus chinensis cell strain with high yield of 10-DAB, is named taxuswallichianavar. mairei plant cell line N12#, and is preserved in CGMCC, and the preservation number is CGMCC No.10001. The invention further discloses screening and establishment of a 10-DAB high-yield cell line (10-DeacetylbaccatinIII, CASNO: 32981-86-5). A high-yield 10-DAB cell line is induced and screened from taxuswallichianavar. mairei explant. Experimental results prove that the cell line can highly produce taxane diterpenoids such as secondary metabolites 10-DAB and can be used for production of crude drug precursors of paclitaxel and docetaxel.

The invention discloses a high-temperature-resistant high-yield cellulase bacillus subtilis and application thereof. A cellulase production strain is separated from surface soil of a corn straw pile in a Jiangjin area of Chongqing, and the preservation number of the bacillus subtilis is CCTCC NO: M 2020002; a strain Z2 with the best enzyme production performance is finally obtained through enrichment culture, a CMC-Na plate experiment, a lignin plate experiment and an enzyme production experiment, and the obtained strain is subjected to 16S rRNA sequence comparison and identification to determine that the strain is bacillus subtilis Z2. By optimizing the culture medium and the culture conditions, the filter paper enzyme activity, the CMC enzyme activity and the beta-glucosidase activity under the optimal enzyme production conditions of 0.800 U / ml, 5.20 U / ml and 2.07 U / ml are obtained respectively. Cellulase produced by the strain has relatively high activity and stability under the conditions of a buffer solution with the pH value ranging from 4.0 to 10.0 and the temperature of 30 DEG C and 80 DEG C. Cellulase produced by the strain is mixed with commercial xylanase, then a saccharification experiment is carried out on pretreated straw stalks, and 84.27 mg / ml of total reducing sugar can be obtained.

The invention relates to a method and equipment for continuous solid-state fermentation and gas-stripping heat-pump coupling separation of a product. The method comprises the following steps: utilizing spiral conveyors to realize continuous charging and discharging; utilizing gas stripping solid-state fermentation to separate a fermentation product online; obtaining the fermentation product through the coupling separation of a heat pump and the gas stripping; and recycling the fermentation product entrained in an exhaust gas by an absorption system. The method has the advantages that: the continuous production of the solid-state fermentation is realized; the gas stripping fermentation eliminates product inhibition and improves the mass transfer and the heat transfer of a system; the fermentation, the gas stripping and the hot pump form a heat closed-loop cycle system, and the energy consumption of the system is low; and the system has low water consumption and no pollution to realize clean production, so the method and the equipment have wide adaptability and great promotion significance.

The invention relates to a method for producing alkali protease from highland bacillus. A strain of highland bacillus is used as an alkali protease fermenting strain, and the adopted culture medium has the formula that every liter of liquid nutrient medium contains 40-80g of bean pulp, 30-70g of sucrose, 0.3-0.7g of tween-80, 20g of glucose, 0.7g of calcium chloride, 0.4g of magnesium sulfate, and 0.3g of potassium chloride. The culture temperature is 37 DEG C, the initial pH value is 10.0, the stirring speed is 300rpm, the inflation speed is 1vvm, and the fermentation time is 3 days. The fermentation liquor is then filtered by a filter press, ultrafiltered and concentrated, then a stabilizing agent and a preservative are added, and the alkali protease is obtained after packaging. The highland bacillus is used as the alkali protease fermenting strain; alkaline-treated soybean meal, sucrose and tween-80 added in the culture medium formula play a pivotal role in improving the enzyme activity of the alkali protease in the fermentation liquor; and soybean peptide has the functional characteristics that the soybean peptide is convenient to digest and absorb by the human body, is antioxidant and antibacterial and the like. The alkali protease produced in the method disclosed by the invention is suitable for producing the soybean peptide.

The invention relates to the technical field of fermentation, and discloses a method for fermentation production of vitamin C precursor 2-keto-L-ulonic acid. The method is that the mixing of Go fungus and Kv fungus forms one-step coversion from D-sorbitol to 2-keto-L-ulonic acid, simplifies the production technology, shortens the fermentation cycle, and reduces the production cost. Meanwhile, the mixed culture of the Go fungus and the Kv fungus is simple, is not easy to cause microbiological contamination, is stable, can utilize an original fermentation tank to perform fermentation, and is easy for realizing industrialization. Furthermore, the method for fermentation production of precursor 2-keto-L-ulonic acid adopts gluconobacter oxydans, with shunt metabolism-related genes of sorbose / sorbitol being knocked out, to be matched with the Kv to subjected to mixed fermentation, prevents the Go fungus and the Kv fungus from competitively fighting for substrate sorbose to a certain degree, and improves the conversion efficiency.

The present invention discloses a Bacillus licheniformis-Bacillus subtilis-Lactobacillus casei composite probiotic preparation and a preparation method thereof, and belongs to the technical field of feed microorganisms, wherein soybean meal, wheat bran and corn powder are adopted as matrixes, and Bacillus licheniformis-Bacillus subtilis-Lactobacillus casei are adopted to carry out solid state fermentation to obtain the probiotic preparation. According to the present invention, the preparation method has characteristics of low production cost, economic benefit increase and simple operation, and can be widely used for the probiotic solid state fermentation technology in the microecology field; the bacterial mixing solid state fermentation is adopted, and the mutual-benefit symbiotic synergism effect among the strains is adopted, such that the yield and the bacterial concentration can be increased, the number of live bacteria in the obtained fermented product is high, and the proteins in the soybean meal and the corn powder are easily decomposed into small peptides and free amino acids so as to be easily digested and absorbed by intestine and stomach; and when the product is added to a feed, the feed utilization efficiency can be effectively increased by 13-25% when the product is fed to milk cow.

The invention belongs to the technical field of beverage processing and relates to a preparation method of a Gewasi beverage. The preparation method comprises the following steps of preparing a wort fermentation broth and a breadcrumb fermentation broth, carrying out isometric mixing of the wort fermentation broth and the breadcrumb fermentation broth, and carrying out filtration, disinfection, blending and carbon dioxide-filling low-temperature pressure-maintaining loading. The preparation method urilizes lactobacillus bulgaricus and beer yeast to ferment a barley malt saccharified liquid and a bread saccharified liquid. The lactobacillus bulgaricus and beer yeast ferment are respectively under optimal conditions so that a fermentation rate is fast and a mouthfeel is good. Compared with preparation methods of the traditional Russian Gewasi beverages, the preparation method provided by the invention has the advantages of stable batch product quality, difficult bacterial infection, low cost, convenient operation and industrial production easiness.

The invention discloses a method for screening cellulase producing strains and a method for producing cellulase by means of fermentation. The cellulase producing strains which are named as TP-02 are acquired from rotten wood in ecological forest in the Huangshang Mountain by means of primary screening, protoplast preparation, ultraviolet-irradiation mutation breeding and secondary screening. The methods have the advantages that the cellulase producing strains are high in cellulase producing capacity, bean pulp, ammonium sulfate, urea or ammonium chloride can be used as nitrogen sources, bran juice, lactose, starch sugar, rice straw or corn straw can be used as carbon sources, the nitrogen sources and the carbon sources are subjected to liquid-state fermentation to obtain the cellulase producing strains, the enzyme activity of filter paper can reach 16.98 IU / mL after the cellulase producing strains are cultivated for 72-84 hours, the enzyme activity of incision enzymes can reach 41.33 IU / mL, the enzyme activity of excision enzymes can reach 3.65 IU / mL, and the enzyme activity of beta-glucanase can reach 15.98 IU / mL.

The bioreactor includes a cylinder and a transmission mechanism and has top cover to seal the cylinder, cup-shaped outer magnetic tank connected to the motor shaft, coaxial inner magnetic tank, stirring shaft with one end fixed to the inner tank and the other end extending beyond the top cover, and positioning bearing for the stirring shaft and inside a bearing box fixed to the top cover. Both the outer and inner magnetic tanks are made of permanent magnetic material while the outer parts of non-magnetic material. Compared with available technology, the present invention has the advantages ofsealed cylinder, complete sterilization, easy bearing installation etc.

The invention relates to a male urinal. The male urinal comprises a urinal body, wherein two slots are formed in the upper end of the urinal body; a paper extraction box for holding clean tissues and a waste paper box for holding waste paper are arranged in the two slots, respectively; out-of-the-box springs are arranged in the slots; the paper extraction box and the waste paper box are pressed to cover the out-of-the-box springs, and the paper extraction box and the waste paper box can be pressed to rise or fall; when the paper extraction box or the waste paper box is flush with the upper end of the urinal body when going down; an in-the-box spring is further arranged inside the paper extraction box and used for storing clean tissues; when the tissues are full, the in-the-box spring is compressed, and as the clean tissues are reduced, the in-the-box spring rebounds gradually; the paper extraction box and the waste paper box both are made of a food grade stainless steel material. Compared with the prior art, the ale urinal has the advantages of simple structure, simple and elegant appearance, relatively low cost, wide applicability, convenience for use, long service life and the like.

The invention relates to the field of microbial fermentation, and in particular relates to a bacillus coagulans solid-state fermentation production method. The method comprises the following steps: (1) first-stage seed preparation; (2) second-stage solid-state seed inoculation; (3) third-stage solid-state seed inoculation; and (4) solid-state fermentation inoculation, wherein a formula of a solid-state fermentation material comprises the following components in percentage by mass: 70-99% of wheat bran, 1-30% of soybean meal, 0.1-10% of glucose, 0.1-10% of urea, 0.1-10% of calcium carbonate and 0.1-5% of manganese sulfate, and accessory materials are added corresponding to the mass of the wheat bran and the soybean meal. According to the method disclosed by the invention, the solid-state fermentation inoculation quantity can be reduced by 50-95% compared with that of the prior art, and a pH value is kept between 7 and 8 in a fermentation process; moreover, a high bacterium number can be achieved, dominant populations can be formed for fermentation, bacterial contamination cannot be easily caused, relatively high spore numbers and spore rates can be achieved, the spore number is 1*10<9>CFU / g to 1*10<11>CFU / g, and the maximum spore rate can reach 99%; and the method is easy in production and promotion.

The invention discloses an electrochemically active strain separating method and an electrochemical activity detecting method, relates to microorganism separating and detecting methods, and solves the problems that separating culture mediums of electrochemically active strains have simple nutrition, are unfavorable for the growth of the electrochemically active strains, and has low electrochemical activity and success ratio of the separated strains, complex detecting operations of electrochemical strain activity and long filtering period. The separating method comprises the steps of: (1) accumulation cultivation, (2) doubling dilution, (3) Hungate rolled tube separation and (4) electrochemically active strain purification. A circular voltammetry method is adopted for detecting electrochemical strain activity. A liquid culture medium of the electrochemically active strain separating method has abundant nutrition, favorable growth of the electrochemically active strains, very intuitive strain size and morpha observation and high separating success ratio; and the circular voltammetry method adopted for detecting the electrochemically active strains has simple operations and shortened filtering period.

The invention discloses bacillus subtilis engineering bacteria and application thereof. Whole genome sequencing comparative analysis shows that SFA-H43 and a D-ribose high-producing strain SFR-4 are higher in homology, and the nucleotide sequences of transketolase gene sequences except mutation sites are totally the same. According to the bacillus subtilis engineering bacteria disclosed by the invention, by adopting the way of homologous recombination, a transketolase gene mutation sequence of an SFR-4 strain is shifted into an SFA-43 strain so as to replace a normal sequence of transketolase genes of the SFA-43 strain, so that a transketolase mutant engineering strain SFR-43T is obtained; the transketolase mutant engineering strain SFR-43T not only maintains good fermentation properties (good stress resistance, fast glucose consumption speed, vigorous growth and difficulty in bacterial infection) of the SFA-43 strain, but also is capable of accumulating D-ribose, and the fermentation stress resistance is obviously increased.

The invention relates to golden cordyceps and lotus seed thick liquor. The golden cordyceps and lotus seed thick liquor is prepared through the method of peeling and washing fresh lotus seeds, cooking the lotus seeds at normal temperature, conducting cooling, putting the lotus seeds in a plastic tank with an interlayer to be compacted, sealed and subjected to heat preservation, adding water to the lotus seeds, grinding the mixture into lotus seed pulp, adding amylase, cellulose and hemicellulase for heat preservation and enzymolysis, conducting plate-frame pressure filtration to obtain lotus seed juice, adding nutrients such as glucose and yeast extract to the lotus seed juice, conducting sterilization, inoculating a cordyceps taishanensis seed solution, conducting cultivation for several days to obtain cordyceps mycelium fermentation liquor, adding white granulated sugar to regulate the sugar degree, adding lactic acid bacterium seed liquid and high-activity dry yeast, conducting filtration and clarification after anaerobic fermentation, adding konjac oligosaccharides, conducting mixing, and conducting sterilization through filter mycoderm to obtain the cordyceps and lotus seed thick liquor. The golden cordyceps and lotus seed thick liquor is reasonable in formula, golden yellow in color, sticky and smooth in taste and strong in ester fragrance and has the functions of improving human immunocompetence and the like. In the thick liquor, the content of cordyceps polysaccharide is larger than 0.4 g / 100 mL, the content of cordycepin is larger than 0.05 g / 100 mL, and viscosity is larger than 200 mPa.s.

The invention relates to a snow pear cough-relieving syrup and a preparation method, which belongs to the composite traditional Chinese medicine technology field. The snow pear cough-relieving syrup solves the problem that the current drugs and transfusion has poor therapeutic effect for rapidly treating cough. The snow pear cough-relieving syrup comprises the following raw materials: 5-15 parts of balloonflower root, 5-15 parts of bitter apricot kernel (fried), 5-15 parts of radix peucedani, 10-25 parts of radix asteris (roasted), 10-25 parts of Common Coltsfoot Flower, 10-30 parts of loquatleaf and 100-300 parts of pear paste. The method comprises the following steps: taking white pear or pyrus ussuriensis, squeezing to obtain the juice, decompressing and concentrating to obtain a stiff paste, employing an ethanol cold leaching method on the other medicinal materials for extracting an effective ingredient, preparing to obtain the stiff paste, adding 700g of cane sugar and other auxiliary materials to obtain the syrup. The snow pear cough-relieving syrup has the advantages of good effect for cough-relieving, good mouthfeel, accurate administration amount and difficult contamination. The snow pear cough-relieving syrup is used for moistening lung, relieving cough and reducing phlegm, and has obvious effect and good security for treating cough, expectoration and bronchitis.

The invention discloses a microbial immobilization method, comprising immobilization for lactic acid bacteria and a continuous production process for Nisin. The microbial immobilization method comprises the following steps of: firstly, growing thalli in a reactor and adsorbing the thalli onto carrier materials; secondly, releasing the free thalli and adding fresh culture solution, and controlling a flow speed to enable the amount of the product Nisin to be the highest when fermentation solution just arrives at the bottom of the reactor and flows out by combining with the height-diameter ratio of the reactor; and finally, enabling the product to flow in a product collection tank, wherein the carriers used are reusable polyurethane foam carrier materials; the whole process is simple to operate; and because two restrictive factors influencing the growth and the production of lactic acid bacteria, namely, nutritional ingredient reduction and low pH caused by the product, are eliminated, continuous production for lactic acid bacteria under a high Nisin yield can be realized.

The invention discloses a preparation method and an application of an immobilized lactobacillus. The preparation method includes the steps: S1 lactobacillus concentrated solution preparation: centrifugally concentrating lactobacillus suspension after enrichment culture to obtain lactobacillus concentrated solution; S2 lactobacillus immobilization: 1) swelling sericin protein by water to obtain sericin protein solution, uniformly mixing the sericin protein solution and the lactobacillus concentrated solution to obtain liquid A; 2) dripping a cross-linking agent into the liquid A acquired in thestep 1) to obtain sericin protein hydrogel containing lactobacilli; 3) dissolving cellulose powder in water to obtain swelling cellulose solution, adding the swelled cellulose solution into the sericin protein hydrogel acquired in the step 2), uniformly mixing mixture, and crosslinking the mixture; 4) slowly dripping calcium chloride solution into crosslinked solution acquired in the step 3) through a constant-flow pump, filtering mixture, washing the mixture by deionized water to obtain the immobilized lactobacillus, and storing the immobilized lactobacillus in a refrigerator with the temperature of 4 DEG C. The immobilized lactobacillus is used for preparing gamma-aminobutyric acid in a fermented manner.

The invention provides a ginkgo leaf capsule. The ginkgo leaf capsule comprises, by weight, 30 to 50 parts of a ginkgo leaf extract, 120 to 180 parts of beta-cyclodextrin, 7 to 12 parts of talcum powder and 0.5 to 1.5 parts of magnesium stearate. A granule is prepared from the above-mentioned raw materials and then fills a hard capsule shell so as to prepare the ginkgo leaf capsule. The ginkgo leaf capsule prepared in the invention has more stable performance and a better clinical curative effect. The invention further provides a preparation method for the ginkgo leaf capsule. The method comprises the following steps: preparing spraying slurry from beta-cyclodextrin and ethanol; subjecting the raw materials to one-step drying and granulation by using a fluid bed granulating and drying machine; and filling the obtained granule to prepare the capsule. The method can overcome disadvantages of the prior art and substantially improves bioavailability, solubility and chemical and physical stability of a drug.

The invention discloses a hyaluronic acid-containing vola repairing and moisturizing spray and a preparation method thereof. The hyaluronic acid-containing vola repairing and moisturizing spray is prepared from super-active sodium hyaluronate, medium and low molecular weight sodium hyaluronate, zinc hyaluronate, low molecular weight zinc hyaluronate, centella asiatica extract, vitamins, amino acids, a preservative and purified water. The preparation method of the spray comprises the following steps: weighing the components, dissolving, filtering and filling into a spray bottle to prepare the water-soluble spray. The spray can be used for foot chapped symptoms commonly existing in life, and has the effects of foot moisturizing, bacteriostasis, inflammation diminishing and repairing. Super-active sodium hyaluronate, medium and low molecular weight sodium hyaluronate, zinc hyaluronate, low molecular weight zinc hyaluronate and centella asiatica are combined according to a certain mass ratio and can interact with one another, so that a synergistic effect is achieved, and the antibacterial and anti-inflammatory effects of the hyaluronic acid-containing vola repairing and moisturizing spray are remarkably improved.

The invention belongs to the technical field of medicines, health-care products and feed processing, and particularly relates to a method for co-producing ellagic acid and a biological feed by fermenting pomegranate rind through microbial communities. According to the method, the pomegranate rind is pretreated by adopting an anaerobic solid-state fermentation technology, cellulose and hemicellulose are degraded, the compact structure of the pomegranate rind is opened to enrich and extract the ellagic acid, the extraction rate of the ellagic acid is improved, and the method has the advantages of low cost, simple process and no pollution in the preparation process; meanwhile, the pomegranate rind filter cake is dried at low temperature to obtain the biological feed, so that the obtained biological feed has relatively strong oxidation resistance on one hand, is beneficial to digestion, absorption and utilization of animals after being subjected to microbial fermentation on the other hand, and can generate lactic acid flavor substances, so that the biological feed becomes sour and fragrant, the palatability of the animals is enhanced, and the additional value of agricultural products is improved; and certain economic benefits are achieved.

The invention discloses a method for preparing cellulase preparation by vinasse of white spirit. The method comprises the following steps of: grafting microorganisms in a fermented material consisting of fresh vinasse of white spirit and brans, and fermenting, drying and smashing to obtain the cellulase preparation, wherein the mass ratio of fresh vinasse of white spirit to brans is 7:3, the grafting amount of microorganisms is 1-2% of the fermented material by weight, and the microorganisms are obtained by mixing cellulase producing bacteria and brewer yeast by weight at a ratio of 4:1. The fermenting temperature is 28-35 DEG C, and the fermenting time is 72-108 hours. Drying is natural drying or drying at temperature lower than 40 DEG C, and the moisture is smaller than or equal to 14%. The mixture is smashed to 20-25 meshes. According to the invention, waste of vinasse of white spirit is effectively utilized by microorganism fermenting technology, so that the environmental pollution is reduced, and the technical support to sustainable production and cleaned production of brewing white spirit is provided.

The invention discloses an agar solution, which comprises agar and alkali, wherein the concentration of agar is 2-3%(w / v), the concentration of alkali is no less than 0.8mol / L. The invention also provides a detection kit including the above mentioned agar solution and a detection method of the kit. The kit is employed for detecting the antibacterial peptide activity, the detection result is accurate and reliable, and the kit has low requirement for the experiment condition, the operation is simple, ordinary people can use the kit, and the practicality is strong.

The invention discloses a preparation method of an acid-resistant high-temperature-resistant alpha-amylase. The preparation method includes selecting genetically-modified bacillus subtilis and subjecting the genetically-modified bacillus subtilis to plate culture, shake-flask culture, seed tank fermentation, fermentation tank fermentation and enzyme extraction so as to obtain the acid-resistant high-temperature-resistant alpha-amylase. The preparation method has the advantages that the genetically-modified bacillus subtilis cannot produce spores and is high in adaptability, less prone to contamination, high in activity and short in period; by adjusting air capacity control and culture medium ratio and effectively controlling a pH value in a preparation process, the prepared acid-resistant high-temperature-resistant alpha-amylase with the optimum reaction temperature of 90-100 DEG C, the pH value of 4.0-5.5, the enzyme activity of 40,000 U / mL and a fermentation period only lasting for 120 hours is a current domestic acid-resistant amylase maximum in enzyme activity within the shortest fermentation period.