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Method for preparing cellulase preparation by vinasse of white spirit

A technology of cellulase and liquor, which is applied in the field of preparation of cellulase preparations, can solve the problems of high input cost, easy corruption, and low utilization rate of liquor waste, and achieve low investment and operation costs, fast microbial growth, and easy The effect of infection

Inactive Publication Date: 2013-06-12
JIANGSU YANGHE BREWERY JOINT STOCK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has the disadvantages of easy corruption, inconvenient storage, and unsuitable for long-distance transportation.
The current distiller's grains are generally used as feed for livestock, or some high value-added products are extracted; as feed, the utilization rate of distiller's grains is low and it is easy to cause secondary environmental pollution; and the extraction of high value-added products, although the waste grains are effectively used , but the initial investment cost is too high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: Weigh 700 kg of fresh liquor, 300 kg of bran, 16 kg of cellulase-producing bacteria, and 4 kg of Saccharomyces cerevisiae. After the materials are fully and uniformly mixed, they are placed on a curtain for fermentation. The stacking height is 20 cm, the fermentation temperature is 28 ° C, and the fermentation time After 108 hours, after the fermentation, it was dried naturally, the water content was ≤14%, and it was pulverized into 20 meshes to obtain the cellulase preparation.

Embodiment 2

[0010] Example 2: Weigh 700 kg of fresh distilled grains of liquor, 300 kg of bran, 8 kg of cellulase-producing bacteria, and 2 kg of Saccharomyces cerevisiae. After the materials are fully and uniformly mixed, they are placed on a solid fermentation rack for fermentation. The stacking height is 25 cm, and the fermentation temperature is 35 ° C. The fermentation time is 72 hours, and after the fermentation is finished, it is dried naturally, the water content is ≤14%, and it is crushed to 25 meshes to obtain the cellulase preparation.

Embodiment 3

[0011] Example 3: Weigh 700 kg of fresh liquor, 300 kg of wheat bran, 12 kg of cellulase-producing bacteria, and 3 kg of Saccharomyces cerevisiae. After the materials are fully and evenly mixed, they are placed in a disc koji making machine for fermentation. The stacking height is 22 cm and the fermentation temperature is 32 °C. , the fermentation time is 90h, after the fermentation is finished, the dryer is dried below 40°C, the water content is ≤14%, and the cellulase preparation is obtained by crushing 22 meshes.

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PUM

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Abstract

The invention discloses a method for preparing cellulase preparation by vinasse of white spirit. The method comprises the following steps of: grafting microorganisms in a fermented material consisting of fresh vinasse of white spirit and brans, and fermenting, drying and smashing to obtain the cellulase preparation, wherein the mass ratio of fresh vinasse of white spirit to brans is 7:3, the grafting amount of microorganisms is 1-2% of the fermented material by weight, and the microorganisms are obtained by mixing cellulase producing bacteria and brewer yeast by weight at a ratio of 4:1. The fermenting temperature is 28-35 DEG C, and the fermenting time is 72-108 hours. Drying is natural drying or drying at temperature lower than 40 DEG C, and the moisture is smaller than or equal to 14%. The mixture is smashed to 20-25 meshes. According to the invention, waste of vinasse of white spirit is effectively utilized by microorganism fermenting technology, so that the environmental pollution is reduced, and the technical support to sustainable production and cleaned production of brewing white spirit is provided.

Description

technical field [0001] The invention relates to a method for preparing cellulase preparations, in particular to a method for preparing cellulase preparations by discarding distiller's grains. Background technique [0002] Liquor waste, as the waste of Chinese liquor brewing, produces about 35 million tons every year. It has the disadvantages of easy corruption, inconvenient storage, and unsuitable for long-distance transportation. The current distiller's grains are generally used as feed for livestock, or some high value-added products are extracted; as feed, the utilization rate of distiller's grains is low and it is easy to cause secondary environmental pollution; and the extraction of high value-added products, although the waste grains are effectively used , but the initial investment cost is too high. Contents of the invention [0003] The object of the present invention is to: provide a method for preparing cellulase preparation by discarding distilled grains, util...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12R1/865
Inventor 张龙云
Owner JIANGSU YANGHE BREWERY JOINT STOCK
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