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Preparation and preparation of Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum

A technology of Bacillus subtilis and Bacillus licheniformis, applied in the direction of bacteria, microorganisms, animal feed, etc., can solve the problems of large environmental pollution, difficult post-processing, cumbersome operation of machinery and equipment, etc., achieve simple equipment structure, increase production rate and bacteria body concentration, simplified fermentation equipment, and easy-to-control effects

Active Publication Date: 2016-06-29
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment

Method used

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  • Preparation and preparation of Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum
  • Preparation and preparation of Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum
  • Preparation and preparation of Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The probiotic preparation prepared by Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum comprises strain cultivation and solid-state fermentation cultivation thereof.

[0039] The solid-state fermentation culture method is as follows: on the solid-state fermentation medium, first inoculate the seed liquid of Bacillus licheniformis BL-09 and Bacillus subtilis HM-66, each inoculation amount is 3.0% (v / w), and the fermentation temperature is controlled at 35° C., humidity 75%, mixed bacteria culture for 12 hours; then add Lactobacillus plantarum HM-10 seed liquid, the inoculation amount is 1.0% (v / w), and other conditions remain unchanged, and then ferment for 12 hours, and the total fermentation time is 24 hours. At the end of the fermentation, the dry culture was obtained by fluidized bed drying method.

[0040] The solid-state fermentation medium is composed of soybean meal, bran and corn flour. The mass ratio of the soybean meal, bran and corn flour...

Embodiment 2

[0043] The probiotic preparation prepared by Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum comprises strain cultivation and solid-state fermentation cultivation thereof.

[0044] The solid-state fermentation culture method is as follows: the solid-state fermentation medium is first inoculated with Bacillus licheniformis BL-09 and Bacillus subtilis HM-66 seed solution, the inoculum amount is 3.0% (v / w) respectively, and the fermentation temperature is controlled at 37°C and the humidity 75%, mixed bacteria culture for 12h; then add Lactobacillus plantarum HM-10 seed liquid, the inoculation amount is 1.0% (v / w), other conditions remain unchanged, and then ferment for 24h, after the end of the fermentation, dry it by fluidized bed drying method Cultures.

[0045] The solid-state fermentation medium is composed of soybean meal, bran and corn flour. The mass ratio of the soybean meal, bran and corn flour is: soybean meal, bran and corn flour=10:9:1, and the ...

Embodiment 3

[0048] The probiotic preparation prepared by Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum comprises strain cultivation and solid-state fermentation cultivation thereof.

[0049] The solid-state fermentation culture method is as follows: on the solid-state fermentation medium, first inoculate the seed liquid of Bacillus licheniformis BL-09 and Bacillus subtilis HM-66, each inoculation amount is 5.0% (v / w), and the fermentation temperature is controlled at 30° C., and the humidity is 75%, mixed bacteria culture for 24 hours; then add Lactobacillus plantarum HM-10 seed liquid, the inoculation amount is 3.0% (v / w), other conditions remain unchanged, and then ferment for 24 hours, after the fermentation is completed, dry Cultures.

[0050] The solid-state fermentation medium is composed of soybean meal, bran and corn flour. The mass ratio of the soybean meal, bran and corn flour is: soybean meal, bran and corn flour=10:9:0.8, and the water content is contro...

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Abstract

The invention belongs to the micro biotechnical field of feed and relates to a probiotics preparation which is prepared by solid fermentation of probiotics bacillus licheniformis BL-09, bacillus subtilis HM-66 and lactobacillus plantarum HM-10 which are mixed in stages by taking bean pulp, bran and corn flour as a matrix. According to the invention, the yield and cell concentration can be improved by adopting mixed bacteria solid state fermentation. The ability of multiple strains to yield enzymes, acids and nutritional substances and the capacity of decomposition to the matrix and the like are remarkably superior to single bacterial fermentation. The product which cannot be obtained by pure fermentation can be obtained, the preparation has the advantages of fast growing speed, high primer utilization ratio, conversion in multiple stages, stable fermented microbial population, labor and energy saving, simplified process and fermentation equipment, easiness in control and the like, and is not easy to contaminate. By fermenting soybean meal, bran and corn flour by the three bacterial strains, proteins in the soybean meal and corn flour can be decomposed to small peptides and free amino acids to facilitate digestive absorption by intestines and stomach.

Description

technical field [0001] The invention belongs to the technical field of feed microorganisms, and relates to a kind of probiotics Bacillus licheniformis BL-09 (Bacillus licheniformis BL-09), Bacillus subtilis HM-66 (Bacillus subtilis HM-66) and plant The probiotic preparation prepared by solid-state fermentation of Lactobacillus plantarum HM-10 (Lactobacillus plantarum HM-10) and the preparation method thereof can be widely used in aspects such as animal microecology. Background technique [0002] At present, most probiotic products are fermented in liquid state, and the probiotic cells are collected directly to make probiotic powder, tablet or capsule. The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment. How to obtain strains suitable for solid-state fermentation and prepare probiotic preparations with a high number of viable ba...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/10C12R1/125C12R1/25
Inventor 赵树平张善亭姚国强
Owner INNER MONGOLIA SCI PLUS BIOTECH
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