Method for producing alkali protease from highland bacillus

Abstract

The invention relates to a method for producing alkali protease from highland bacillus. A strain of highland bacillus is used as an alkali protease fermenting strain, and the adopted culture medium has the formula that every liter of liquid nutrient medium contains 40-80g of bean pulp, 30-70g of sucrose, 0.3-0.7g of tween-80, 20g of glucose, 0.7g of calcium chloride, 0.4g of magnesium sulfate, and 0.3g of potassium chloride. The culture temperature is 37 DEG C, the initial pH value is 10.0, the stirring speed is 300rpm, the inflation speed is 1vvm, and the fermentation time is 3 days. The fermentation liquor is then filtered by a filter press, ultrafiltered and concentrated, then a stabilizing agent and a preservative are added, and the alkali protease is obtained after packaging. The highland bacillus is used as the alkali protease fermenting strain; alkaline-treated soybean meal, sucrose and tween-80 added in the culture medium formula play a pivotal role in improving the enzyme activity of the alkali protease in the fermentation liquor; and soybean peptide has the functional characteristics that the soybean peptide is convenient to digest and absorb by the human body, is antioxidant and antibacterial and the like. The alkali protease produced in the method disclosed by the invention is suitable for producing the soybean peptide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing alkaline protease by using bacillus highterus. Background technique [0002] Protease is the most widely used and most used enzyme in industrial biological enzyme preparations. Its current market usage accounts for about 60% of the total biological enzymes, of which alkaline protease accounts for 25%. In addition to being widely used in the detergent industry, protease is also widely used in many industries such as food processing, chemical industry, medicine and light industry, showing huge market application value, and its application range is still expanding, especially in soybean Production of functional food additives such as peptides, etc. [0003] Since the 1950s, my country began to screen alkaline protease-producing strains. After continuous selection and breeding, there has been great progress in the research and production of alkaline p...

AI Technical Summary

Problems solved by technology

In recent years, the domestic demand for soybean peptides has grown rapidly, but due to insufficient investment in protease research and production related to the application of soybean peptides in my country, there are problems such as low unit enzyme activity and unsatisfactory hydrolyzed products.

However, the price of related protease products of foreign companies has been high, resulting in an increase in the cost of enzyme use, which has become a bottleneck restricting the development of my country's soybean peptide industry.

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