This invention discloses a method for establishing a high-efficiency regeneration
system for *Pyrus pyrifolia* leaves, belonging to the field of
plant tissue culture technology. The method includes: spreading a layer of
sorbitol solution on the surface of a culture medium; inoculating *Pyrus pyrifolia* seedlings into the medium and placing them in a dark environment for
etiolation treatment; taking etiolated leaves from the *Pyrus pyrifolia* seedlings and inoculating them in a regeneration medium for dark culture, then transferring them to light for further culture to produce adventitious buds; after the adventitious buds reach the target length,
cutting off their lateral buds and placing them on a subculture medium to obtain regenerated seedlings. This invention, by adding
sorbitol solution before dark treatment, can significantly shorten the time to obtain etiolated material to 7 days, and the etiolated plants are in better condition after three weeks; furthermore, by placing *Pyrus pyrifolia*
tissue culture seedlings in a dark environment for dark culture, the physiological state of the
tissue culture seedlings is altered, and the obtained etiolated leaves are inoculated into the regeneration medium, improving the regeneration efficiency, and the material acquisition is not affected by the season.