34 results about "Pyrus bretschneideri" patented technology

The invention discloses a method for shortening the juvenile period of directive breeding hybrid seedlings of Pyrus bretschneideri Rehd. The method adopts sowing and greenhouse seedling raising in the same year, and comprises the following steps: removing seed coat, carrying out gibberellin treatment, spraying GA3 in the growing period of the hybrid seedlings, conducting inversed ring-bark grafting on the hybrid seedlings sprayed with GA3, carrying out hybrid seedling terminal bud grafting, dwarfing stock, spraying chlormequat chloride (CCC), and various other technical measures, so as to shorten the juvenile period of the directive breeding hybrid seedlings of Pyrus bretschneideri Rehd. According to the method, the Pyrus bretschneideri Rehd. hybrid seedlings can bloom and fruit in the third year after being planted, and the fruiting plant rate can be 30 percent, while the conventional Pyrus bretschneideri Rehd. hybrid seedlings bloom and fruit 7 to 8 years after being planted. Being treated with the six measures, the Pyrus bretschneideri Rehd. directive breeding hybrid seedlings can fruit in the third year after being planted, that is, the juvenile period is shortened by 4 to 5 years. Therefore, the Pyrus bretschneideri Rehd. directive breeding efficiency is greatly improved; the Pyrus bretschneideri Rehd. directive breeding period is shortened; a new way and a new method are provided for breeding of new Pyrus bretschneideri Rehd. directive breeding varieties; the application value is high.

The invention discloses a method for efficiently and rapidly regenerating adventitious buds from pear leaves. The method comprises steps as follows: acquiring sterile tissue culture seedlings, generating adventitious buds by inducing the leaves and performing primary culture and subculture on the adventitious buds, wherein an inducing medium is a combination of a basic culture medium NN69, auxin IBA and cytokinin TDZ, the leaf age is about 20-50 d, dark culture is performed for 21 d, the regeneration effect of leaves of Pyrus bretschneideri Rehd. is remarkable, the regeneration rate can reach 70.83%, the average number of regeneration buds of each leaf is 2.06, the callus occurrence rate reaches 100%, the browning rate is very low and is even 0, and demands for germplasm preservation of pear varieties and requirements for the regeneration rate of the Pyrus bretschneideri Rehd. serving as a genetic transformation material are met. Requirements for the leaves are low, the material sources are enriched, and the problem of high browning rate in the woody plant tissue regeneration process is solved. The problem that the regeneration rate of the oriental pear varieties is generally low is effectively solved, an effective material is expected to be provided for genetic transformation of the oriental pear varieties, and breakthrough of genetic transformation of pears is realized.

The invention discloses a molecular identification method of HMGR gene mRNA in pear stock-scion transfer. The molecular identification method comprises the following steps: (1) by taking pyrus bretschneideri rehd as scion and pyrus betulifolia bunge as stock, carrying out micro grafting of test-tube seedlings; (2) extracting total RNA of pyrus bretschneideri rehd, pyrus betulifolia bunge and grafted stock and scion, carrying out reverse transcription to obtain cDNA, carrying out PCR amplification on the total length of the HMGR gene, and carrying out second-time PCR by adopting the amplification result as a template, thus obtaining respective HMGR gene fragments, wherein the sequences of upstream and downstream primers obtained through two times of PCR are respectively shown as SEQ ID NO.1-4; and (3) carrying out enzyme digestion on the PCR product obtained for the second time by adopting restriction endonuclease, and comparing enzyme digestion spectrograms before and after grafting for identifying the transfer condition. The method provided by the invention is rapid and sensitive, is high in accuracy, is simple and convenient, and can be applied to other pear plants.

The invention provides a compost for auricularia auricular, and belongs to the technical field of compost for edible fungi cultivation. The compost is mainly prepared by fermenting main raw materials such as pear tree branches and leaves, persimmon tree branches and leaves, rosebay branches and leaves, bombax cotton branches and leaves, yam bean vines and traditional Chinese medicine dregs, and auxiliary raw materials such as wheat bran, gypsum powder, quicklime powder, potassium dihydrogen phosphate, white sugar and vitamin C. The compost is comprehensive and rich in nutrition and high in quality, also has certain anti-bacterial capability and sterilization capability, is capable of effectively restraining infection of infectious microbes, reducing production of disease and insect damage, and promoting realization of high and stable-yield planting of the auricularia auricula, and is free from public hazards, pollution and residues.

The invention discloses a method for reinforcing trunks of trees in a labor-saving manner for cultivating the pyrus bretschneideri rehd trees. The method is characterized by comprising land selecting and preparing, variety selecting, field planting, grafting, tree management and training and pruning. Compared with the prior art, the method has the advantages that trunk merits of the trees can be controlled, tree crowns can be shaped fast, the trees are simple in structure and convenient to cultivate, field management can be facilitated, fruit growers can easily master the method, existing tree structures can be effectively changed, the stress resistance of the pyrus bretschneideri rehd trees can be improved, the method has important popularization value, and the like.

The invention provides wheat bran and fruit biscuits and a processing method thereof. The processing method comprises the following steps: pulverizing wheat bran, enabling the pulverized wheat bran to be sieved by a sieve with the meshes of 30, enabling the sieved wheat bran to be mixed with hawthorn powder and medicated leaven powder uniformly to obtain a mixture, puffing and drying the mixture, and pulverizing the mixture to obtain puffed fine powder with meshes of 160-200 for subsequent use; adding water in liquorice, boiling the water and then decocting the liquorice on soft fire, filtering decoction, and retaining soup for subsequent use; mixing the puffed fine powder, the soup, starch, sugar, konjak powder, banana powder, mulberry powder, jujube powder, fructus lycii powder, fructus cannabis powder, grape powder, pyrus bretschneideri powder, the liquorice, salt, baking soda and stevioside, stirring uniformly, processing into a dough, fermenting the dough, then processing into biscuit flans, and baking the biscuit flans in an oven to obtain the wheat bran and fruit biscuits. The wheat bran and fruit biscuits have the advantages that the content of dietary fibers is quite high, the nutrition is abundant, the wheat bran and fruit biscuits taste sweet, homology of medicine and food is implemented, the health-care function is fulfilled, and particularly, the wheat bran and fruit biscuits have quite good treatment effect on intractable constipation.

The invention discloses a malic acid transporter gene PbrTDT1 from pyrus bretschneideri and application of the malic acid transporter gene PbrTDT1, and belongs to the technical field of plant geneticengineering. The novel malic acid transporter gene PbrTDT1 is obtained by cloning from pyrus bretschneideri fruits, a nucleotide sequence of the malic acid transporter gene PbrTDT1 is shown as SEQ IDNO: 1 in a sequence listing, and a coded amino acid sequence of the malic acid transporter gene PbrTDT1 is shown as SEQ ID NO: 2 in the sequence listing. The gene is transformed into tomatoes throughagrobacterium-mediated genetic transformation to obtain transgenic plants, and biological function verification shows that the malic acid content in fruits of the transgenic tomatoes is obviously increased, which indicates that the cloned PbrTDT1 gene has the function of regulating and controlling the malic acid content of fruit pulp. The TDT gene PbrTDT1 from the pyrus bretschneideri can be applied to fruit acidity character improvement or high-acid transgenic fruit construction, and provides a new gene resource for molecular breeding for improving the quality of plant fruits.

The invention discloses preserved dragon blood chicken and a preparation method thereof, and belongs to the technical field of foods. The preserved dragon blood chicken is prepared by taking chicken as a raw material, and taking five spice seasoning, preserved dragon blood, vegetable oil and salt as auxiliary materials. The preserved dragon blood chicken is prepared from, in parts by weight, 95-105 parts of chicken, 0.5-1.5 parts of five spice seasoning, 0.6-1 parts of dragon blood, 8-10 parts of preserved pyrus bretschneideri, 8-10 parts of preserved hawthorn, 8-10 parts of preserved litchi, 8-10 parts of preserved apple, 8-10 parts of preserved yellow peach, 8-10 parts of preserved mango, 10-20 parts of vegetable oil and 1-2 parts of salt. The preserved dragon blood chicken disclosed by the invention combines chicken with the preserved dragon blood, and is unique in flavour, short in production period, high in efficiency, good in product taste, non-greasy, and especially suitable for being eaten by the female.

The invention discloses an organic planting method for pyrus bretschneideri and relates to the technical field of pear tree planting. The method comprises the following steps of soil improvement, planting, fertilizer and water management and pest and disease control. Soil is improved first, base fertilizer is applied twice, and leguminous plants are overturned and pressed to serve as green manure,so that fertilization is promoted, the soil fertility is improved, the micro-ecological environment of an orchard is improved, the yield of fruit trees is increased, and the quality of the fruit trees is improved; the bottoms of planting pits are backfilled with the organic base fertilizer, so that the permeability of roots of fruit tree seedlings is improved, and absorption of nutrients by the roots is strengthened; a combination of plant-derived insecticidal paste and a plant-derived insecticide is adopted so that green leafhoppers, pear psylla, oriental fruit moths, black spots and powderymildew can be effectively prevented and treated, and less pollutants are caused to the environment. The organic planting method can effectively reduce environmental pollution and is conductive to restoring of ecological balance, and planted pyrus bretschneideri is good in quality, high in yield, juicy in pulp, delicate in texture, less in stone cell and residue and sweet, sour and delicious in taste.

The invention relates to the field of food processing, in particular to a canned Gonggan making method. Canned Gonggan is prepared from, by weight, 40-45% of Gonggan, 20-25% of pyrus bretschneideri and 30-40% of slurry. The making method comprises the steps of material preparation, rinsing, steam treatment, cooling, alkalization treatment, canning and sterilization. By the adoption of the canned Gonggan making method, the crisp, tender, fresh and sweet taste of Gonggan pulp can be maintained, rich nutrients are contained, various vitamins are contained, nutrients and water required by the human body can be effectively provided, and the canned Gonggan can generate greater economic benefits than Gonggan fruits.

The invention relates to a Beijing pyrus bretschneideri rehd wine, and belongs to the technical field of food, wherein the Beijing pyrus bretschneideri rehd wine comprises, by weight, 1000 g of Beijing pyrus bretschneideri rehd flesh, 1-10 g of pectinase, 60-80 g of a concentrated pear juice, 50-80 g of pear paste, 20-50 g of honey, 1-10 g of a yeast nutrient, 2-10 g of dried yeast powder, and 1000 g of purified water. According to the present invention, the Beijing pyrus bretschneideri rehd wine has characteristics of sweet, sour and delicious taste and mellow taste, can provide effects of beautifying, physical fitness strengthening, lung nourishing and lung moisturizing, does not has any additives and preservatives, and has no side effect on the human body.

The invention discloses an edible coating agent, and a preparation method and a use method thereof, and belongs to the technical field of fruit fresh-keeping. The coating agent comprises 120-180 parts of calcium chloride, 180-320 parts of starch, 150-230 parts of Arabic gum powder, 0.5-2 parts of garlicin and 30000 parts of water. The use method comprises the following steps: completely immersing picked Pyrus bretschneideri Rehd. fruits with no mechanical damages and no pest diseases in the edible liquid coating agent for 5-10 s, taking out the fruits, airing the fruits in a shady place, processing the aired fruits according to routine packaging and cartonning operation rules, moving the cartoned fruits to a cold store, and refrigerating the fruits according to routine refrigeration processing rules to realize prolongation of the fresh-keeping period of the Pyrus bretschneideri Rehd. fruits and the improvement of the storage quality.

The invention relates to Pyrus bretschneideri Rehd.cv. Tse-Li syrup as well as a preparation method and applications of the Pyrus bretschneideri Rehd.cv. Tse-Li syrup in removing hepatic calculus. The Pyrus bretschneideri Rehd.cv. Tse-Li syrup is prepared by heating and concentrating 7-14 jin of Pyrus bretschneideri Rehd.cv. Tse-Li juice and one part of a traditional Chinese medicine decoction. The Pyrus bretschneideri Rehd.cv. Tse-Li syrup is characterized in that the one part of traditional Chinese medicine decoction is decocted from the following raw materials in parts by weight: 5-20 g of bulbus fritillariae cirrhosae, 5-15 g of buckwheat, 5-20 g of fructus ziziphi jujubae, 3-10 g of dried rehmannia root, 5-10 g of radix puerariae, 5-30 g of Raphanus sativus L., 3-15 g of radix ophiopogonis, 10-30 g of radix glehniae, and 2000-4000g of water. Good-quality syrup is decocted from good-quality pears, and good medicines are prepared from good-quality syrup. Compared with the prior art, the Pyrus bretschneideri Rehd.cv. Tse-Li syrup has the efficacies of moistening lung to arrest cough, clearing the throat, decreasing internal heat and generating body fluid, harmonizing stomach and reducing phlegm, maintaining beauty and keeping young, and eliminating toxins, and also has the efficacy of naturally removing hepatic calculus without appetite regulation.

The invention provides a manually assisted hand-held triangular block variable-diameter branch clamping pear girdler, including a handle, a spring, a rotating shaft, a triangular clamping block, and a fixed circle. A spring is fixedly installed on the handle, and the spring is a tension spring. At the same time, the force of the spring firmly fixes the triangular clamping block on the branch of the fragrant pear to prevent it from rotating with the girdling knife. The handle is divided into two parts, the fixed circle is installed on the handle, and the two parts of the fixed circle are fastened together. Together they form a complete circle, the handle opens and closes, and the fixed circle also opens and closes accordingly. There is a groove on the fixed circle, the roller is embedded in the groove and rolls in the groove, and the link runs through the center hole of the roller , the roller rotates in a circle, the connecting rod connects the two fragrant pear girders together, the girdling handle is fixed on the connecting rod, one end of the girdling handle is provided with a girdling knife, and the other end is provided with a connecting plate. Next, the girdling knife takes the fixed point of the handle and the link as the center of the circle, and uses the fixed point to the tip of the girdling knife as the radius to make a circular motion to gird the fragrant pears.

The invention provides a Chinese herbal medicine with an immunity enhancing function and a preparing method thereof. The Chinese herbal medicine comprises, by weight, 3-7 parts of bur, 10-20 parts ofhoneysuckle, 5-15 parts of paris polyphylla, 7-12 parts of fritillaria, 8-15 parts of coptidis rhizoma, 7-12 parts of cortex phellodendri, 8-13 parts of radix scutellariae, 12-17 parts of radix ophiopogonis, 10-15 parts of common selfheal fruit-spike, 10-17 parts of radix angelicae sinensis, 7-13 parts of brucea javanica, 10-18 parts of artemsia indica, 4-9 parts of licorice root, 12-18 parts of dried radix rehmanniae, 10-15 parts of dried tangerine peel, 2-7 parts of cordyceps sinensis, 10-20 parts of endothelium corneum gigeriae galli, 7-12 parts of frankincense, 8-13 parts of myrrh, 12-18 parts of hive, 12-18 parts of dendrobe, 9-15 parts of fuligo e herbis, 10-17 parts of lucid ganoderma, 3-8 parts of musk, 2-6 parts of calculus bovis, 4-7 parts of pyrus bretschneideri, 1-5 parts of white radish, 1-4 parts of honey and 45-55 parts of lard stock. The obtained Chinese herbal medicine has quite good effects of resisting bacteria and viruses, relieving heat, resisting inflammation, protecting the liver, stopping bleeding and resisting oxidization and can effectively improve the human body blood circulation, remove free radicals in human bodies and enhance the human body immunity.

The invention discloses a brewage method of Pyrus bretschneideri Rehd fruit wine. The brewage method of Pyrus bretschneideri Rehd fruit wine comprise following steps including: raw material selecting;raw material washing; raw material smashing; clarification; ingredient primary adjusting; alcoholic fermentation; ingredient secondary adjusting; fermentation termination; filtering and sterilizing;aging; and filling. The obtained Pyrus bretschneideri Rehd fruit wine is clear and transparent, is moderate in sour sweet taste, possesses rose fragrance; the original nutrients in Pyrus bretschneideri Rehd can be maintained; the additional value of Pyrus bretschneideri Rehd is increased; a novel fruit wine is invented; the Pyrus bretschneideri Rehd fruit wine is welcomed because of the unique flavor; and excellent social benefit and economical benefit are achieved.

The invention discloses a biological preservative and fresh-keeping method based on food safety. The method comprises the steps as follows: 1, before storage, selecting fresh and full vegetables and fruit without mechanical damage; 2, changing the gas composition around; 3, adopting a temperature as low as possible under the precondition that cold damage is avoided; 4, keeping certain humidity to reduce the moisture loss of vegetables and fruit to maintain the freshness and quality of vegetables and fruit; 5, using a biological preservative and fresh-keeping agent prepared from linaria vulgaris extract, pomegranate bark extract, loranthus parasiticus extract, polygala japonica extract, equisetum hyemale extract, chitosan and polyoxyethylene sorbitan fatty acid esters. Experiments carried out on fruit and vegetables like pyrus bretschneideri, oranges and green pepper prove that the method is good in preservative and fresh-keeping effect.

The invention discloses a method for culturing young embryos of Pyrus bretschneideri and relates to Pyrus bretschneideri. In-vitro embryos are cultured by a plant cell culture technology to break dormancy of Pyrus bretschneideri seeds and realize a higher seed germination rate. The method takes unmatured young embryos or mature young embryos of the Pyrus bretschneideri as explants, a culture technology system for in-vitro young embryos of the Pyrus bretschneideri is established by explant sterilization, young embryo culture, rooting culture, acclimatization and transplantation in order to laya foundation for establishing a regeneration system of the Pyrus bretschneideri and improving Pyrus bretschneideri varieties by genetic engineering and cell engineering. The method can significantly shorten the breeding process, improve breeding efficiency, and open up a new breeding way.

The invention relates to a whitening spot-fading composition and an application thereof, and provides a whitening spot-fading facial mask with a remarkable effect. The composition with the whitening spot-fading effect provided by the invention includes the following components: 0.001-0.008% of frozen pear extract and 0.001-0.008% of orychophragmus violaceus extract. Through research, the applicants find that the frozen pear extract has better tyrosinase inhibitory activity than an unfrozen pyrus bretschneideri extract. The frozen pear extract and the orychophragmus violaceus extract have a significant synergistic effect after being compounded; and nutrient ingredients are added to a facial mask, so that playing of the whitening spot-fading effect of the frozen pear extract and the orychophragmus violaceus extract can be effectively promoted, and the whitening spot-fading effect of the facial mask can be obviously improved.